Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

• Zahra Nabizadeh,
• Mahmoud Nasrollahzadeh,
• Hamed Daemi,
• Mohamadreza Baghaban Eslaminejad,
• Ali Akbar Shabani,
• Mehdi Dadashpour,
• Majid Mirmohammadkhani and
• Davood Nasrabadi

Beilstein J. Nanotechnol. 2022, 13, 363–389, doi:10.3762/bjnano.13.31

• cardiomyocyte-incorporated CNT–GelMA hydrogel for TE of the cardiovascular system [132]. To fabricate CNT–GelMA nanocomposites, they added MWCNTs into GelMA to improve the electrophysiological and mechanical properties. The electrical conductivity of CNTs allowed the researchers to form nanocomposite 3D

Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension

• Khosro Adibkia,
• Ali Ehsani,
• Asma Jodaei,
• Ezzatollah Fathi,
• Raheleh Farahzadi and
• Mohammad Barzegar-Jalali

Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62

• that induce cardiomyocyte cell cycle arrest and cellular senescence. Hence, finding solutions to increase the TL of cardiac cells could be helpful. We hypothesize that Ag-NPs can increase the cardiomyogenic differentiation potential of BM-MSCs. Also, it seems that Ag-NPs could change the cardiac

• %) and CD34 (0.25%) and positive for CD44 (92.6%) and CD90 (89.4%). Cardiomyogenic differentiation confirmation of BM-MSCs According to previous studies, MSCs can differentiate into cardiomyocyte cells if they are cultured in a specific cardiomyocyte differentiation medium [18][19]. In this section, the

• cardiomyogenic differentiation was confirmed via immunocytochemistry (ICC). Briefly, as shown in Figure 3A–D, when the BM-MSCs were cultured in the cardiomyocyte differentiation medium for a period of 14 days, they exhibited the cardiac markers of α-actinin and desmin. Cell proliferation assay Cardiomyogenically

Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system

• Hiroaki Komuro,
• Masahiro Yamazoe,
• Kosuke Nozaki,
• Akiko Nagai and
• Tetsuo Sasano

Beilstein J. Nanotechnol. 2020, 11, 1685–1692, doi:10.3762/bjnano.11.150

• vector for transfecting cardiomyocyte-derived HL-1 cells. HAp exhibited particles on the nanoscale and with a low cytotoxicity in HL-1 cells. The transfection assay performed with several endocytosis inhibitors suggested that the HAp/pDNA complexes were internalized by HL-1 cells through macropinocytosis

• . Furthermore, this HL-1 cell uptake was generated in response to HAp stimulation. Thus, HAp is a positive regulator of macropinocytosis in HL-1 cells and a good system for gene delivery in cardiomyocytes. Keywords: cardiomyocyte; endocytosis; gene delivery system; hydroxyapatite nanoparticles

• HAp. The HL-1 cells were used as the in vitro model of cardiomyocytes in this study [23]. Since HL-1 cells are derived from mouse atrial myocytes they have an adult cardiomyocyte phenotype with the expression of cardiac-specific functional receptors. Results Characterization of HAp nanoparticles The