Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• ). Reversible addition–fragmentation chain transfer (RAFT) copolymerization of HPMA with MMAA and preparation of hydrazide derivative of P(HPMA-MMAA) In a 50 mL Erlenmeyer flask, CTPA (4 mg), AIBN (5 mg), MMAA (0.1 mL), and HPMA (0.9 g) were dissolved in ethanol (9 mL) under Ar atmosphere, 5 M HCl (50 μL) was

• washed with diethyl ether, and dried; yield: 0.6 g. To transform the polymer to its hydrazide derivative, P(HPMA-MMAA) (0.6 g) was dissolved in ethanol (2 mL) in a 25 mL beaker, hydrazine hydrate (50 μL) was added, and the reaction continued at RT for 16 h with stirring (6 rpm). The viscous P(HPMA-MMAA

• ][20]. In contrast, MMAA introduces to the copolymer the methoxy functional group, which can be subsequently easily transformed to the hydrazide required for conjugation with compounds containing reactive carbonyl groups (aldehyde or ketone), such as Dox. To prepare the MMAA monomer, the reactive

Micro- and nano-surface structures based on vapor-deposited polymers

• Hsien-Yeh Chen

Beilstein J. Nanotechnol. 2017, 8, 1366–1374, doi:10.3762/bjnano.8.138

• resulting multifunctional patterned surface could spatially direct a combination of Huisgen cycloaddition and carbonyl–hydrazide coupling in a sequentially devised immobilization procedure [52]. A similar sequential immobilization of molecules on defined areas was also performed on a layered coating of

Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies

• Xuefeng Wang,
• Zhong Mei,
• Yanyan Wang and
• Liang Tang

Beilstein J. Nanotechnol. 2017, 8, 372–380, doi:10.3762/bjnano.8.39

• dithiolaromatic PEG6-CONHNH2 by specifically reacting with the carbohydrate moiety in the Fc portion of the antibody can direct the conjugating orientation. The compound has a hydrazide and dithiol group at opposite ends, which react with aldylated antibodies by NaIO4 modification and gold nanoparticles via Au–S

Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA)

• Paula Ciaurriz,
• Fátima Fernández,
• Edurne Tellechea,
• Jose F. Moran and
• Aaron C. Asensio

Beilstein J. Nanotechnol. 2017, 8, 244–253, doi:10.3762/bjnano.8.27

• interactions or directional binding by means of a linker (Figure 1a,b). For the first one (adsorption), a protocol was set up regarding previous work on the matter [8][26]. In the case of the directional strategy, a previously described protocol was followed [20]. A hetero-bifunctional linker, hydrazide

• -polyethylene glycol-dithiol, was used to control the orientation of the molecules on the surface of the nanoparticle. Hydrazide is able to react with aldehyde groups that can be generated by oxidizing the carbohydrates of glycosylated proteins, such as antibodies [20]. For this purpose, antibody and HRP

• where 300 µL were used. A curve log(agonist)–response is adjusted to obtained data y = min + (max − min)/(1 + 10log(EC50 − X)). Materials The BSA fraction VI for blocking was purchased from Merck. ELISA Maxisorb plates were acquired from Nunc. The linker PEG6-hydrazide aromatic dialkanedithiol was used

Preparation of alginate–chitosan–cyclodextrin micro- and nanoparticles loaded with anti-tuberculosis compounds

• Albert Ivancic,
• Fliur Macaev,
• Fatma Aksakal,
• Veaceslav Boldescu,
• Serghei Pogrebnoi and
• Gheorghe Duca

Beilstein J. Nanotechnol. 2016, 7, 1208–1218, doi:10.3762/bjnano.7.112

• -pyridinecarboxylic acid hydrazide, Figure 1, compound 1) is a white crystalline powder soluble in water, slightly soluble in ethanol, chloroform and hardly soluble in ether. INH has antimycobacterial properties and is a first-line agent in the treatment of pulmonary and extrapulmonary tuberculosis [19]. ISN (1-[2

Comparison of the interactions of daunorubicin in a free form and attached to single-walled carbon nanotubes with model lipid membranes

• Dorota Matyszewska

Beilstein J. Nanotechnol. 2016, 7, 524–532, doi:10.3762/bjnano.7.46

• commercial, oxidized nanotubes SWCNT(s)-COOH (CheapTubes, Brattleboro, USA) with the diameter of 1–2 nm and length of 5–30 µm. The modification at the ends of the nanotubes as well as in the defect sites was obtained by the formation of SWCNTs-end hydrazide, which was then mixed with daunorubicin to form

Dimer/tetramer motifs determine amphiphilic hydrazine fibril structures on graphite

• Loji K. Thomas,
• Nadine Diek,
• Uwe Beginn and
• Michael Reichling

Beilstein J. Nanotechnol. 2012, 3, 658–666, doi:10.3762/bjnano.3.75

• alkyl chains. The nanoscale morphology is a consequence of the basic molecular geometry, where it follows that a closure to form a fibril is not always likely for the doubly substituted hydrazine. Therefore, we also observe crystallite formation. Keywords: fibrils; graphite; hydrazide; hydrazine