Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• checked using gel electrophoresis. The microarray experiment was performed using oligonucleotide microarrays (Genechip - Primeview human, Affymetrix, USA) that can detect the expression of 49,372 genes. Typically, 500 ng of RNA was converted to double-stranded cDNA. The biotin-labeled complementary RNA

Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP)

• Seyed Mohammad Mahdi Dadfar,
• Sylwia Sekula-Neuner,
• Vanessa Trouillet,
• Hui-Yu Liu,
• Ravi Kumar,
• Annie K. Powell and
• Michael Hirtz

Beilstein J. Nanotechnol. 2019, 10, 2505–2515, doi:10.3762/bjnano.10.241

• spotted onto the surfaces via µCS. The microarrays were incubated for performing antigen–antibody interaction for different times (10 to 60 min) and at different temperatures (room temperature of 25 °C and elevated physiological temperature of 37 °C). Then, the samples were washed and the fluorescence

• signal of the spot array was quantified (Figure 4). As a general trend, higher fluorescence intensities are observed at an elevated temperature of 37 °C for all microarrays at the same incubation time. For room temperature incubation, the observed fluorescence intensity increases with incubation time for

• (bottom) and the epoxy-terminated (top) glasses, h) the epoxy-terminated glass (bottom) and the sample of route 5 (top), i) the epoxy-terminated glass (bottom) and the sample of route 6 (top). Schematic of the microarrays prepared for AFP detection. a) Incubating biotinylated surfaces with streptavidin

High-throughput micro-nanostructuring by microdroplet inkjet printing

• Hendrikje R. Neumann and
• Christine Selhuber-Unkel

Beilstein J. Nanotechnol. 2018, 9, 2372–2380, doi:10.3762/bjnano.9.222

• BCML can intrinsically only coat surfaces with nanoparticle arrays, many biosensor applications also require well-defined structures and concentrations of ligands in microarrays [17]. Particularly the generation of cell arrays is a highly challenging task [18][19]. A feasible strategy would therefore

• also proteins [23][24][25][26][27]. In this way, even flexible materials can easily be patterned [28]. Therefore, with this method, we have the combined benefits of BCML with the advantages of inkjet printing to achieve nanoparticle structures in defined microarrays. Such micro-nanostructures have been

Strategy to discover full-length amyloid-beta peptide ligands using high-efficiency microarray technology

• Clelia Galati,
• Natalia Spinella,
• Lucio Renna,
• Danilo Milardi,
• Francesco Attanasio,
• Michele Francesco Maria Sciacca and
• Corrado Bongiorno

Beilstein J. Nanotechnol. 2017, 8, 2446–2453, doi:10.3762/bjnano.8.243

• Aβ and blocking the early steps of amyloid aggregation. Here, we show the combination of high-efficiency slides (HESs) with peptide microarrays as a promising tool for identifying small peptides that bind Aβ monomers. To this aim, HESs with two immobilized reference peptides, (i.e., KLVFF and Semax

• ) with opposite behavior, were investigated for binding to fluorescently labeled Aβ peptide. Transmission electron microscopy was used to demonstrate Aβ fibrillar aggregates missing. The use of HESs was critical to ensure convenient output of the fluorescent microarrays. The resulting sensitivity, as

• well as the low sample consumption and the high potential for miniaturization, suggests that the proposed combination of peptide microarrays and highly efficient slides would be a very effective technology for molecule profiling in AD drug discovery. Keywords: Alzheimer's disease; florescence; high

Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• variety of bio-applications. Keywords: microcontact cantilever spotting; phospholipids; polymeric porous support; polymethacrylate; Introduction Starting with the creation of high density peptide microarrays in the 1990s [1] the development of arrays with DNA molecules and antibodies has produced a

Anticancer efficacy of a supramolecular complex of a 2-diethylaminoethyl–dextran–MMA graft copolymer and paclitaxel used as an artificial enzyme

• Yasuhiko Onishi,
• Yuki Eshita,
• Rui-Cheng Ji,
• Masayasu Onishi,
• Takashi Kobayashi,
• Masaaki Mizuno,
• Jun Yoshida and
• Naoji Kubota

Beilstein J. Nanotechnol. 2014, 5, 2293–2307, doi:10.3762/bjnano.5.238

• have also been analyzed in melanoma cells over time. These findings, which were obtained from dynamic Bayesian analysis in combination with non-linear regression, were confirmed by using data from DNA microarrays [36]. The survival of PTX-resistant melanoma cells has been analyzed extensively by using

• DNA microarrays and dynamic Bayesian analyses. A mathematical model was generated by dynamic Bayesian networks and nonparametric regression analysis using a supercomputer with 1024 cores. One hour after the administration of PTX, the RBM23 gene, which is a known target of PTX, acted as a hub and

Effect of channel length on the electrical response of carbon nanotube field-effect transistors to deoxyribonucleic acid hybridization

• Hari Krishna Salila Vijayalal Mohan,
• Jianing An,
• Yani Zhang,
• Chee How Wong and
• Lianxi Zheng

Beilstein J. Nanotechnol. 2014, 5, 2081–2091, doi:10.3762/bjnano.5.217

• the field of biomedical and life science research [1][2]. Currently, the detection methods for nucleic acids include northern blotting analysis [3], in situ hybridization [4], real-time fluorescence quantitative polymerase chain reaction (RT-PCR) [5] and microarrays [6]. These detection methods suffer

Porous polymer coatings as substrates for the formation of high-fidelity micropatterns by quill-like pens

• Michael Hirtz,
• Marcus Lyon,
• Wenqian Feng,
• Andrea E. Holmes,
• Harald Fuchs and
• Pavel A. Levkin

Beilstein J. Nanotechnol. 2013, 4, 377–384, doi:10.3762/bjnano.4.44

• the porous HEMA substrates can improve the fidelity and quality of microarrays prepared by using the quill-like microcantilevers. The presented method sets the stage for further studies using chemical and biochemical recognition elements, along with colorimetric and fluorometric sensors that can be

• spotted by this method onto flat porous polymer substrates. Keywords: microarrays; microscale printing; microspotting; polymeric porous support; polymethacrylate; quill-like pens; Introduction Microarrays are of immense importance in many fields of biological research (e.g., genomics, proteomics, and

• cell analysis) and medical applications in diagnostics such as the detection of pathogens or antibodies. Nitrocellulose films and nylon membranes are widely used as carriers for microarrays, usually with spot sizes in the range of 100 to 500 µm, easily accessible for current inkjet and spotting

Distance dependence of near-field fluorescence enhancement and quenching of single quantum dots

• Volker Walhorn,
• Jan Paskarbeit,
• Heinrich Gotthard Frey,
• Alexander Harder and
• Dario Anselmetti

Beilstein J. Nanotechnol. 2011, 2, 645–652, doi:10.3762/bjnano.2.68

• labelled individual molecules. Furthermore, the MDP approach is very well suited for qualitative ad hoc validation of experimental data. The significance of dipolar coupling in single molecule manipulation assays was demonstrated. Conceivable applications range from microarrays to controlled manipulation