pH-mediated control over the mesostructure of ordered mesoporous materials templated by polyion complex micelles

• Emilie Molina,
• Mélody Mathonnat,
• Jason Richard,
• Patrick Lacroix-Desmazes,
• Martin In,
• Philippe Dieudonné,
• Thomas Cacciaguerra,
• Corine Gérardin and
• Nathalie Marcotte

Beilstein J. Nanotechnol. 2019, 10, 144–156, doi:10.3762/bjnano.10.14

• -PAA) or poly(ethylene oxide)-b-poly(methacrylic acid) (PEO-b-PMAA) copolymers, are able to form polyion complex (PIC) micelles upon interaction with weak polybases such as oligochitosan (OC) [25], poly-L-lysine (PLL) [26][27] and aminoglycoside antibiotics [28]. PIC micelles present a core–corona

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• , Karlsruhe, Germany. 0.01% poly(L-lysine) solution (PLL, Mr = 150,000–300,000, sterile-filtered, and cell-culture tested) was purchased from Sigma-Aldrich Chemie Gmbh, Munich, Germany. Sample support microchips with a central silicon nitride (SiN) membrane window of a dimension of 150 × 400 μm and a

Template-controlled piezoactivity of ZnO thin films grown via a bioinspired approach

• Nina J. Blumenstein,
• Fabian Streb,
• Stefan Walheim,
• Thomas Schimmel,
• Zaklina Burghard and
• Joachim Bill

Beilstein J. Nanotechnol. 2017, 8, 296–303, doi:10.3762/bjnano.8.32

• according to Lipowsky et al. with a dipping robot DR 3 from Riegler & Kirstein, Germany [26]. Solutions of poly(styrene sulfonate) (PSS, Sigma-Aldrich, M ≈ 70,000 g mol−1), poly-L-glutamic acid (PLGA, Sigma-Aldrich, M = 15,000–50,000 g mol−1) and poly-L-lysine hydrobromide (PLL, Sigma-Aldrich, M = 15,000

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

• Alexandra M. Greiner,
• Adria Sales,
• Hao Chen,
• Sarah A. Biela,
• Dieter Kaufmann and
• Ralf Kemkemer

Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155

• molecules is disadvantegous too. Using more advanced chemistry for immobilizing the molecules of interest in a controlled fashion [145][146][147][148]. Typical molecules used for non-specific surface coatings are poly-L-Lysine (PLL) and poly(lactic acid) (PLA), both interact unspecifically with cells

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

• Igor M. Pongrac,
• Marina Dobrivojević,
• Lada Brkić Ahmed,
• Michal Babič,
• Miroslav Šlouf,
• Daniel Horák and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84

• . Their properties can be modified by coating with different biocompatible polymers. To test if a coating polymer, poly(L-lysine), can improve the biocompatibility of nanoparticles applied to neural stem cells, poly(L-lysine)-coated maghemite nanoparticles were prepared and characterized. We evaluated

• intracellular uptake of iron oxide in neural stem cells. The methyl thiazolyl tetrazolium assay and the calcein acetoxymethyl ester/propidium iodide assay demonstrated that poly(L-lysine)-coated maghemite nanoparticles scored better than nanomag®-D-spio in cell labeling efficiency, viability and proliferation

• labeling makes poly(L-lysine)-coated maghemite nanoparticles appropriate candidates for future neural stem cell in vivo tracking studies. Keywords: dextran; maghemite; nanoparticles; neural stem cells; poly(L-lysine); Introduction Stem cell-based therapy is a developing area of regenerative medicine with

Surface coating affects behavior of metallic nanoparticles in a biological environment

• Darija Domazet Jurašin,
• Marija Ćurlin,
• Ivona Capjak,
• Tea Crnković,
• Marija Lovrić,
• Michal Babič,
• Daniel Horák,
• Ivana Vinković Vrček and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 246–262, doi:10.3762/bjnano.7.23

• steric effects on the stabilization of NPs. Thus, AgNPs were produced with the following coatings (Figure 1): trisodium citrate (CITAgNP), sodium bis(2-ethylhexyl) sulfosuccinate (AOTAgNP), cetyltrimethylammonium bromide (CTAAgNP), poly(vinylpyrrolidone) (PVPAgNP), poly(L-lysine) (PLLAgNP), bovine serum

• albumin (BSAAgNPs), Brij 35 (BrijAgNP) and Tween 20 (TweenAgNP). The SPIONs were prepared as uncoated γ-Fe2O3 NPs (UNSPIONs), and coated with D-mannose (MANSPIONs) or poly(L-lysine) (PLLSPIONs). Three media for NP dispersion were investigated: ultrapure water (UW), biological cell culture medium without

• bromide (CTA), poly(vinylpyrrolidone) (PVP), poly(L-lysine) (PLL), bovine serum albumin (BSA), Brij 35 (Brij), Tween 20 (Tween) and D-mannose (MAN). Silver nanoparticles coated with sodium bis(2-ethylhexyl) sulfosuccinate (AOTAgNP), cetyltrimethylammonium bromide (CTAAgNP), poly(vinylpyrrolidone) (PVPAgNP

Fabrication and characterization of novel multilayered structures by stereocomplexion of poly(D-lactic acid)/poly(L-lactic acid) and self-assembly of polyelectrolytes

• Elena Dellacasa,
• Li Zhao,
• Gesheng Yang,
• Laura Pastorino and
• Gleb B. Sukhorukov

Beilstein J. Nanotechnol. 2016, 7, 81–90, doi:10.3762/bjnano.7.10

• ) (PLLA) were alternately adsorbed directly on calcium carbonate (CaCO3) templates and on poly(styrene sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) multilayer precursors in order to fabricate a novel layer-by-layer (LBL) assembly. A single layer of poly(L-lysine) (PLL) was used as a linker

• . Experimental Materials Sodium carbonate, calcium chloride, poly(styrene sulfonate) (PSS, MW 70,000), poly(allylamine hydrochloride) (PAH, MW 58,000), poly(L-lysine-hydrobomide) (MW = 30,000–70,000), L-lactide, D-lactide, tannous octoate, ethylenediaminetetraacetic acid (EDTA) were obtained from Sigma-Aldrich

Tattoo ink nanoparticles in skin tissue and fibroblasts

• Colin A. Grant,
• Peter C. Twigg,
• Richard Baker and
• Desmond J. Tobin

Beilstein J. Nanotechnol. 2015, 6, 1183–1191, doi:10.3762/bjnano.6.120

• particle size testing was then deposited on to a poly-L-lysine-coated glass slide for 60 s then washed off and dried in a steady stream of nitrogen. AFM imaging was carried out to examine the tattoo ink particles that were used in the particle size distribution testing. (a) Particle size distribution of

Self-assembled anchor layers/polysaccharide coatings on titanium surfaces: a study of functionalization and stability

• Ognen Pop-Georgievski,
• Dana Kubies,
• Josef Zemek,
• Neda Neykova,
• Roman Demianchuk,
• Eliška Mázl Chánová,
• Miroslav Šlouf,
• Milan Houska and
• František Rypáček

Beilstein J. Nanotechnol. 2015, 6, 617–631, doi:10.3762/bjnano.6.63

• ]. Chitosan/alginate, multilayered, 3D networks prepared by the layer-by-layer method enabled encapsulation of bone marrow stromal cells on the surface of dental or joint implants [18]. Polyelectrolyte (chitosan, poly(L-glutamic acid), and poly-L-lysine) coatings increased the surface ionic nature and

Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces

• Miao Yu,
• Nico Strohmeyer,
• Jinghe Wang,
• Daniel J. Müller and
• Jonne Helenius

Beilstein J. Nanotechnol. 2015, 6, 157–166, doi:10.3762/bjnano.6.15

• SCFS, a single cell is attached to a cantilever (Figure 1A,B), commonly facilitated by an adhesive coating (e.g., concanavalin A, poly-L-lysine or CellTak) [17][18][19][20][21][22]. The attached cell is lowered (approach) onto a substrate (Figure 1A(i)), which is a protein-coated surface, another cell

Synthesis of boron nitride nanotubes and their applications

• Saban Kalay,
• Zehra Yilmaz,
• Ozlem Sen,
• Melis Emanet,
• Emine Kazanc and
• Mustafa Çulha

Beilstein J. Nanotechnol. 2015, 6, 84–102, doi:10.3762/bjnano.6.9

• biomedical applications [78]. The GA–BNNTs were found to be nontoxic to MRC-5 cells, but the previous studies claimed that glucosamine prevented the cellular uptake of glucose by pancreatic beta cells and caused cell death [73]. Poly-L-lysine (PLL) is known for its cytocompatibility due to the presence of

Biopolymer colloids for controlling and templating inorganic synthesis

• Laura C. Preiss,
• Katharina Landfester and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2014, 5, 2129–2138, doi:10.3762/bjnano.5.222

• somehow odd, even more in the case of microgel particles, which are definitely not “hard”. However, in our classification we consider within this group all approaches in which a shell is formed on the surface of a particle. As an example, Boissière et al. [62] synthesized poly(L-lysine)/alginate

Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells

• Michal Babič,
• Daniel Horák,
• Lyubov L. Lukash,
• Tetiana A. Ruban,
• Yurii N. Kolomiets,
• Svitlana P. Shpylova and
• Oksana A. Grypych

Beilstein J. Nanotechnol. 2014, 5, 1732–1737, doi:10.3762/bjnano.5.183

• ., dextran [18][19] (in Feridex® and Endorem® developed as contrast agents for magnetic resonance imaging, MRI), poly(ethylene glycol) (PEG) [1], poly(N,N-dimethylacrylamide) (PDMAAm) [20], poly(L-lysine) [21][22], protamine sulfate [23], or layer-by-layer polyelectrolyte complexes [24]. The aim of this

Non-covalent and reversible functionalization of carbon nanotubes

• Antonello Di Crescenzo,
• Valeria Ettorre and
• Antonella Fontana

Beilstein J. Nanotechnol. 2014, 5, 1675–1690, doi:10.3762/bjnano.5.178

• secondary structure of DNA, a precipitation/redispersion sequence can be obtained upon simply lowering the pH below 4–5 and raising it to 7. The process is totally reversible, nondestructive and can be repeated several times [91]. Worth noting is also the pH response of poly-L-lysine (PLL)/SWCNTs complexes

The softening of human bladder cancer cells happens at an early stage of the malignancy process

• Jorge R. Ramos,
• Joanna Pabijan,
• Ricardo Garcia and
• Malgorzata Lekka

Beilstein J. Nanotechnol. 2014, 5, 447–457, doi:10.3762/bjnano.5.52

• grown previously on a glass coverslip covered with poly-L-lysine. In the present studies no glass surface modification was introduced. Thus, substrate chemical properties do not change the relationship between non-malignant HCV29 and cancerous T24 cells. The comparison performed in the previous studies

Growth behaviour and mechanical properties of PLL/HA multilayer films studied by AFM

• Cagri Üzüm,
• Johannes Hellwig,
• Narayanan Madaboosi,
• Dmitry Volodkin and
• Regine von Klitzing

Beilstein J. Nanotechnol. 2012, 3, 778–788, doi:10.3762/bjnano.3.87

• Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany 10.3762/bjnano.3.87 Abstract Scanning- and colloidal-probe atomic force microscopy were used to study the mechanical properties of poly(L-lysine)/hyaluronan (PLL/HA)n films as a function of indentation velocity and the

• relatively harder one [3][6][25]. Richert et al. showed that chondrosarcoma cells adhere much more strongly on chemically cross-linked poly(L-lysine)/hyaluronic acid PLL/HA films than on native PLL/HA films due to the elasticity difference between the two structures, the cross-linked surface being harder [6

• biological processes [3]. In this work, scanning- and colloidal-probe AFM were used to perform nanoindentation on poly (L-lysine)/hyaluronan (PLL/HA)n films with n = 12–96, in order to better understand their growth behaviour, apparent Young’s modulus, and viscoelastic properties. Results and Discussion