Photorelease of phosphates: Mild methods for protecting phosphate derivatives

  1. § ,
  2. and
Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, U.S.A
  1. Corresponding author email
§ Present address: Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, U.S.A.
¶ Present address: Department of Chemistry, Sweet Briar College, Sweet Briar, VA 24595, U.S.A.
Guest Editor: P. R. Hanson
Beilstein J. Org. Chem. 2014, 10, 2038–2054. https://doi.org/10.3762/bjoc.10.212
Received 22 May 2014, Accepted 07 Aug 2014, Published 29 Aug 2014
Full Research Paper
cc by logo

Abstract

We have developed a new photoremovable protecting group for caging phosphates in the near UV. Diethyl 2-(4-hydroxy-1-naphthyl)-2-oxoethyl phosphate (14a) quantitatively releases diethyl phosphate upon irradiation in aq MeOH or aq MeCN at 350 nm, with quantum efficiencies ranging from 0.021 to 0.067 depending on the solvent composition. The deprotection reactions originate from the triplet excited state, are robust under ambient conditions and can be carried on to 100% conversion. Similar results were found with diethyl 2-(4-methoxy-1-naphthyl)-2-oxoethyl phosphate (14b), although it was significantly less efficient compared with 14a. A key step in the deprotection reaction in aq MeOH is considered to be a Favorskii rearrangement of the naphthyl ketone motif of 14a,b to naphthylacetate esters 25 and 26. Disruption of the ketone-naphthyl ring conjugation significantly shifts the photoproduct absorption away from the effective incident wavelength for decaging of 14, driving the reaction to completion. The Favorskii rearrangement does not occur in aqueous acetonitrile although diethyl phosphate is released. Other substitution patterns on the naphthyl or quinolin-5-yl core, such as the 2,6-naphthyl 10 or 8-benzyloxyquinolin-5-yl 24 platforms, also do not rearrange by aryl migration upon photolysis and, therefore, do not proceed to completion. The 2,6-naphthyl ketone platform instead remains intact whereas the quinolin-5-yl ketone fragments to a much more complex, highly absorbing reaction mixture that competes for the incident light.

Introduction

Phosphates have long held an important formative position in the development of organic photochemistry beginning with the seminal report by Havinga [1] of the unusual substituent effects in the photosolvolysis of aryl phosphates that showed unexpectedly pronounced meta activation of substituted aryl phosphates. A strong ‘meta effect’ resulting in enhanced reaction efficiency by electron withdrawing, meta substituents is contrary to their ground state effects on solvolysis reactions which, instead, display enhanced reactivity for para substituents. This contrasting substituent reactivity is a consequence of two different potential energy surface (PES) contours that control the reactivity, an observation first rationalized by Zimmerman through a comparison of the change in PES electron distribution in the ground (HOMO) and excited states (LUMO) using Hückel molecular orbital theory [2,3], subsequently attributed by him to the positioning of conical intersections between the HOMO–LUMO PE surfaces [4,5]. He termed this phenomenon the “meta effect”, and generalized it for photochemical solvolysis reactions. Several additional photosolvolysis studies substantiated the generality of the meta effect for phosphate leaving groups in heterolytic photoreactions.

Over the past four decades, additional examples of phosphate esters attached to reactive chromophores other than phenyl and benzyl [6-11] have been investigated for their propensity to undergo heterolytic photosolvolysis reactions, most notably o-nitrobenzyl (o-NB) [12], benzyl phenyl ketone (benzoin) [13,14], coumarin-4-ylmethyl [15], and, more recently, p-hydroxyphenacyl (pHP) [15] phosphate esters (Figure 1). While these chromophores exhibit a range of photophysical properties, all share a conjugated aromatic structural motif that facilitates UV–vis absorption and serves as a traceless reagents, orthogonal to common ground state deprotection processes and essentially independent of pH effects. Thus, the chromophores are particularly useful at neutral pH and no added reagents are required for protecting group release.

[1860-5397-10-212-1]

Figure 1: Common photoremovable protecting groups (PPGs) for phosphates depicted as diethyl phosphate (DEP) esters.

Many photosolvolysis studies have targeted key biological substrates [6,8-12] for mechanistic and phenomenological studies. The culmination of these studies has resulted in the development of an entirely new branch of photochemistry with application in both organic chemistry and biochemistry. The reagents are generally described as “caged” compounds or photoremovable protecting groups (PPGs) in which the attached chromophore masks the biological activity of a substrate. Irradiation releases the substrate, allowing it to return to its normal bioactivity.

The desirable properties of any new PPG candidate are 1) absorption at wavelengths near or above 400 nm, 2) enhanced chemical and photochemical quantum yields and 3) improved rate of release, ideally in the range of ps to ns time constants; all three are useful properties for applications in organic chemistry and biochemistry, but they are particularly important for mechanistic studies in biochemistry and biophysics [6,8-11]. Most PPG candidates require sufficiently high energy excited states for heterolysis of a carbon–oxygen or carbon–heteroatom bond that binds the chromophore to a substrate. This limits the useful absorption range to ca. 350 to 400 nm for heterolysis by a primary photochemical pathway.

Phosphates hold a central historic position in caged photochemistry through their cross-disciplinary significance in both biology and chemistry. Nucleotides (especially ATP, cAMP, and GTP) were among the first to be covalently bound to chromophores in a cage or PPG format that were demonstrably released upon photolysis. Benzoin caged cAMP and o-nitrophenethyl caged ATP, seminal examples of caged phosphates, are often the two classic caged biochemical substrates cited [6-13,15-17]. Subsequent interest in and application of PPGs has exploded largely because they provide researchers in interdisciplinary fields with a tool for initiating biological [18] and chemical processes [6,8,10,11] by remote control with light as the activator. Spatial, temporal and concentration parameters for releasing substrates are controlled by adjusting the focus, time resolution, and intensity of the light source in conjunction with other variables such as the photochemical reactivity and molecular reaction pathway. Since photolysis reactions are generally considered to be kinetically much faster than ground state processes (most photorelease rates have sub μsec time constants), the use of time resolved lasers to activate PPGs offers much greater temporal resolution and more precise spatial control of the reaction variables in chemical processes, increasingly important features for biophysics and biochemistry.

Our studies on photosolvolysis reactions have uncovered several new chromophores with superior PPG properties amenable to photodeprotection. Earlier work defined the advantages and limitations of p-hydroxyphenacyl (pHP, 4), a PPG that is now finding application in biochemistry and chemistry [15]. We now report new pHP analogs with a fused aromatic or heteroaromatic extension of pHP. Our intent was to impose the pHP motif on the naphthyl and indolin-5-yl platforms (maintaining the critical substituent pattern) in order to foster aryl ketone migration by a photo-Favorskii rearrangement concomitant with ligand release. The combination of these features shifts and extends the chromophore, exhibiting a more intense, broader absorption band closer to the visible region, making the PPG more accessible for photodeprotection. Thus, elaborating the two motifs by optimally appending a hydroxy and carbonyl positioned for excited state interaction, in accord with the pHP motif 4a, invited serious examination.

Results

Synthesis of phosphate esters

A series of diethyl phosphate (DEP) esters caged with extended 2-(6-hydroxynaphthalen-2-yl)-2-oxoethyl (2,6-HNA, 10), 2-(4-hydroxynaphthalen-1-yl)-2-oxoethyl (1,4-HNA, 14a) and its methoxy ether (1,4-MNA, 14b), and 8-(benzyloxy)quinolin-5-yl)-2-oxoethyl (5,8-BQA, 24), each modeled after the p-hydroxyphenacyl (pHP, 4) chromophore were synthesized as illustrated in Scheme 1 and Scheme 2.

[1860-5397-10-212-i1]

Scheme 1: Synthesis of 2,6-HNA DEP (10), 1,4-HNA DEP (14a), and 1,4-MNA DEP (14b) DEP esters. Reagents and conditions: a) PhSH, K2CO3, N-methyl-2-pyrrolidinone, 194 °C, 45 min; b) TBSCl, Et3N, DBU, CH2Cl2, rt, 28 h; c) CuBr2, 1:1 CHCl3/EtOAc; d) KHSO4, MeOH (aq), CH2Cl2, rt, 43 h; e) tetramethylammonium diethyl phosphate, DMF, 55 °C, 2–3 h; f) Ac2O, AlCl3, CH2Cl2, 0 °C to rt over 3 h then stirred at rt.

Synthesis of the extended 2,6-HNA phosphate ester 10 was accomplished by first demethylating 2-acetyl-6-methoxynaphthalene (5) with thiophenol and 0.1 mol percent potassium carbonate in N-methyl-2-pyrrolidinone at 194 °C [19] to generate the hydroxynaphthalene 6 (72%) followed by protecting the hydroxy group with TBSCl to give 7 (89%, Scheme 1A). α-Bromination with copper(II) bromide [20] gave 8 (96%, direct bromination of 6 gave poor yields). The TBS group was removed in 30% aqueous methanol containing 10–20% methylene chloride and several molar equivalents of potassium hydrogen sulfate to provide hydroxy bromo ketone 9 (yield, 75%) [21], which was reacted with tetramethylammonium diethyl phosphate under anhydrous conditions to afford diethyl (2-(6-hydroxynaphthalen-2-yl)-2-oxoethyl) phosphate (2,6-HNA DEP, 10, 62%) [22].

Diethyl (2-(4-methoxynaphthalen-1-yl)-2-oxoethyl) phosphate (1,4-MNA DEP, 14b) was obtained, as shown in Scheme 1B, beginning with Friedel–Crafts acylation of 1-methoxynaphthalene (11) yielding 1-acetyl-4-methoxynaphthalene (12, 73%). α-Bromination with copper(II) bromide gave 13 (44%) which was converted to the phosphate ester using tetramethylammonium diethyl phosphate in dimethoxyethane (DME) at room temperature to give diethyl (2-(4-methoxynaphthalen-1-yl)-2-oxoethyl) phosphate (1,4-MNA DEP, 14b, 92%).

The hydroxy analog (1,4-HNA DEP, 14a, Scheme 1C), was synthesized from methoxynaphthone 12 by demethylation with thiophenol giving acetylnaphthol 15 in moderate yield (42%). Protection with TBSCl gave 16 (86%) followed by α-bromination to 17 (89%). TBS deprotection gave 2-bromo-1-(4-hydroxy-1-naphthyl)ethanone, (18, 59%) which was converted to diethyl (2-(4-hydroxynaphthalen-1-yl)-2-oxoethyl) phosphate (1,4-HNA DEP, 14a, 31%) with tetramethylammonium diethyl phosphate.

Benzyl protected diethyl 2-(8-hydroxyquinolin-5-yl)-2-oxoethyl phosphate (24) was synthesized from 8-hydroxyquinoline (19) by first performing acylation [23] with acetyl chloride and then quantitatively protecting the hydroxy group as its benzyl ether [24] followed by installation of ketal protection with iodobenzene diacetate in alkaline methanol [25] to give 22 (90%, Scheme 2). Deprotection of 22 with 50% acetic acid gave the α-hydroxy ketone 23 (98%) which was esterified with diethyl phosphoryl chloride in pyridine [26] to afford 2-(8-(benzyloxy)quinolin-5-yl)-2-oxoethyl diethyl phosphate (5,8-BQA DEP, 24, 30%).

[1860-5397-10-212-i2]

Scheme 2: Synthesis of diethyl 8-(benzyloxy)quinolin-5-yl)-2-oxoethyl phosphate (5,8-BQA DEP, 24). Reagents and conditions: (a) CH3COCl, AlCl3, nitrobenzene, 14 h, 40%; (b) PhCH2Br, K2CO3, DMF, rt, 24 h, 100%; (c) PhI(OAc)2, MeOH, KOH, 0 °C to rt, 24 h, 90%; (d) 50% CH3COOH, rt, 12 h, 98%; (e) P(O)Cl(OEt)2, pyridine, −5 °C to rt, 24 h, 30%.

The UV–vis spectra of 1,4-substituted esters 14a,bmax range from 319 to 325 nm) are substantially red-shifted relative to the pHP ester 4amax at 287 nm) and exhibit molar extinction coefficients (ε) of ca. 104 M−1cm−1 in aq MeCN (Figure 2). For the 2,6-HNA DEP (10) and 2,6-HNA GABA, the λmax appears at 325 nm whereas the λmax for the 5,8-BQA phosphate 24 occurs at 321 nm in 10% aq MeCN. For the 2,6-HNA series, a strong fluorescence emission is observed at 470 nm as shown here for the more aqueous soluble GABA ester derivative. The 2,6-HNA GABA had better aqueous solubility for fluorescence studies. (See Supporting Information File 1 for synthetic and spectral details).

[1860-5397-10-212-2]

Figure 2: A. UV–vis spectrum of 14a (1,4-HNA DEP) in 1% aq MeCN. B. Fluorescence emission/excitation spectra of 2,6-HNA GABA (0.042 mM) in pH 7.3 TRIS buffer containing 1% MeCN.

Photolysis of phosphate esters

Irradiation of 14a at 350 nm in Pyrex vessels under ambient conditions in 1% aq CD3OD resulted in the release of diethyl phosphate as confirmed by NMR (Scheme 3, Table 1). The protecting group underwent a photo-Favorskii rearrangement (Scheme 4), yielding predominantly methyl 4-hydroxy-1-naphthylacetate (25) with a trace amount of reduction product 15 (Scheme 3). In anhydrous methanol, the ratio of 25/15 was approximately 3:1. When the photolysis was performed in 10% aq MeCN, 15 was the only assigned structure from the 1H NMR spectrum of a complex product mixture.

[1860-5397-10-212-i3]

Scheme 3: Photolysis of 1,4-HNA and 1,4-MNA diethyl phosphates 14a and 14b in aq MeOH.

Table 1: Photolysis efficiencies and product yields for 1,4-HNA DEP (14a) and 1,4-MNA DEP (14b).

ester % conversiona deprotection
% yieldb
Φdisc
14a OH 98 100 0.028
14b OMe 40 90 0.0076

aOne hour photolysis in CD3OD at 350 nm, determined by 1H NMR using DMF as an internal standard, bdiethyl phosphate, corrected for conversion, cdetermined by RP-HPLC; photolysis in 1% aqueous MeOH.

[1860-5397-10-212-i4]

Scheme 4: The photo-Favorskii rearrangement of 14a.

Photolysis of the methoxy analog 14b under similar conditions either in CD3OD or 1% aq MeCN resulted in the release of DEP and formation of 12 as confirmed by reversed-phase HPLC (RP-HPLC) and NMR (Scheme 3, Table 1) but the conversion was not complete and the reaction was lethargic due to competitive absorption by the unrearranged product chromophore 12. In 1% aq MeOH, however, the major product from the protecting group was methyl 4-methoxy-1-naphthylacetate (26) which was characterized by the signals at δ 4.02 and 3.68 ppm and confirmed by FAB–MS (m/z 230) and comparison with an independently synthesized sample obtained from Friedel–Crafts alkylation of 1-methoxynaphthalene (11) with methyl bromoacetate and ferric oxide [27].

Quantum yields for disappearance and percentage conversions for 14a are higher than that of 14b as indicated in Table 1. After one hour of photolysis at 350 nm in CD3OD, 14a reached 98% conversion whereas 14b managed to attain only 40% conversion. The disappearance quantum efficiency (Φdis) for 14a in 1% aq CD3OD was 0.028, more than three times that for 14b under the same conditions. However, the deprotection yields for both esters were excellent, resulting in quantitative release of DEP based on conversion.

The quantitative conversion and apparent good efficiency of the 1,4-derivative 14a prompted further investigation. The quantum yield for disappearance of 14a in 1% aq MeCN (Φdis = 0.021) is comparable to that observed in aq MeOH (Φdis = 0.028, Table 1 and Table 2) but the product mixture was more complex (vide infra). Interestingly, quantum yields for the disappearance for 14a are enhanced three-fold (Φdis = 0.067) when the photolysis solution was purged free of oxygen (Table 2). The quantum yield of 14a for the triplet sensitized photolysis in the presence of benzophenone (BP), a well-established triplet sensitizer, at 254 nm under O2-free conditions was Φdis = 0.022. These combined results demonstrate that the reactive excited state is a triplet that is partially quenched by O2 under ambient conditions.

Table 2: Photolysis of 1,4-HNA diethyl phosphate (14a) in the presence and absence of O2 and benzophenone in 1% aq MeCN.

Φdisa conditions
0.067b degassed with Ar
0.022 BP sensitizedc
0.021 saturated with Air

aDetermined with RP-HPLC; bEstimated value (see experimental section); cBenzophenone (11 mM) as the sensitizer and 14a (0.59 mM) in 1% aq MeCN at 254 nm and purged with Ar.

Irradiation of 2,6-HNA DEP (10) at 350 nm in 1% aq MeCN under conditions comparable to those employed with 14a,b also released phosphate. The disappearance quantum yield for 10dis = 0.031) was nearly the same as that obtained for 14a but the conversion after a 10 min irradiation was only 67%. Unlike 14a,b, however, the chromophore did not undergo the photo-Favorskii rearrangement, forming only the reduction product 2-acetyl-6-hydroxynaphthalene (6) in addition to other unidentified photoproducts (Scheme 5). These results paralleled our observations for 14a,b in aq MeCN.

[1860-5397-10-212-i5]

Scheme 5: Photolysis of 2,6-HNA DEP (10) in 1% aq MeCN.

Finally, photolysis of 5,8-BQA diethyl phosphate (24) in 10% aq MeCN at 300 or at 350 nm under degassed conditions released DEP at only 30% conversion, even after 18 hours as confirmed by both 1H and 31P NMR analyses (Scheme 6). The quantum yields for disappearance and product appearance (Φdis = Φapp = 0.00024) were exceptionally low (Table 3), considerably less than any of the naphthyl (10 and 14) or phenacyl (4a) analogs (see Table 4, Discussion section). A complex product mixture of byproducts of the indolinyl chromophore was obtained which was not investigated further.

[1860-5397-10-212-i6]

Scheme 6: Photolysis of 5,8-BQA diethyl phosphate (24).

Table 3: Quantum yields and conversions of 24 with and without Ar purging.a

ester % conversionb
ambient O2
% conversionb
Ar purged
Φdis (× 103)c
ambient O2
Φapp (× 103)d
ambient O2
24 32 30 0.24 0.24

aSolvent was 10% H2O in MeCN. Photolyses were conducted with 300 or 350 nm lamps. bDetermined using 1H NMR in 10% D2O in CD3CN; cDetermined with 1H NMR for the disappearance of the starting material at 350 nm with air; dDetermined with 1H NMR for the appearance of diethyl phosphate at 350 nm with air.

Discussion

In our design of new PPG chromophores, we have attempted to circumvent or avoid the inherent deficiencies of the classical o-NB, benzoin, and coumaryl analogs (see Figure 1). A noisome limitation of o-NB PPGs that mitigate their general use in biological and mechanistic studies are their inherently slow reaction rates. o-NB rate constants are decidedly among the slowest for a photochemical heterolysis (e.g., usually not larger than μsec time constants) due to mechanistic constraints imposed by the rate determining step of a ground state hemiacetal hydrolysis, generated photochemically, that releases the substrate. Furthermore, the o-NB PPG chromophore, itself, is converted to an o-nitroso aldehyde (or ketone) that reacts with endogenous nucleophiles such as amines, thiols, etc. either present on the substrate, on target proteins or in the media. Poignant evidence of these limitations surfaced during mechanistic investigations of GAP protein GTPases and enzymatic studies of ATPase [28-34].

We [15,28,29,35] and others [6-11] have successfully offered alternatives to the o-NB PPG series, initially with benzoin phosphate 2 [13,14] and coumarin-4-ylmethyl phosphate 3. However, the chromophores for these candidates remain intact or rearrange to more strongly absorbing chromophores which compete for the effective radiation wavelengths, often resulting in incomplete conversions that compromise their synthetic utility. These chromophores and especially their photoproducts are also highly fluorescent, which frequently prove to be disadvantageous.

The recent candidates are based on a pHP motif. Phosphate esters of 10, 14, 24, and acetate 27 [19] each possess a hydroxy donor coupled with an acetyl function bearing the leaving group (Figure 3). One of these, the 5-hydroxy-1-naphthylacetyl motif as its acetate ester 27, has already been reported to be photochemically inert in 1:1 aq MeCN by Wan [19]. Accordingly, we did not pursue the study of 27 (vide infra). Extending the aromatic portion of the chromophore with either an added benzo or pyridyl group using the pHP model red-shifted the chromophore absorption for the remaining three, 10, 14, and 24 (e.g., the maximum of 4a at 280 nm shifted to 325 nm for 14a tailing nearly to 400 nm, see Figure 2).

[1860-5397-10-212-3]

Figure 3: Naphthyl and quinolin-5-yl caged phosphate esters 10, 14, 24 and 27 (acetate ester).

Similar extensions of the benzyl PPG expanding it to naphthylmethyl [35,36] (28 and 29, Figure 4) or modifications by attaching substituents to phenacyl (e.g., 4b,c) or benzyl (30bd) moieties, and naphthylacetyl [37] reportedly lowered the singlet and triplet state energies only modestly [10,11] and were unsuitable because these chromophores remained intact and in competition for incident radiation.

[1860-5397-10-212-4]

Figure 4: Previously studied caged diethyl phosphate PPGs possessing aromatic (benzyl, phenacyl, and naphthylmethyl) phosphates.

Photolysis of 1,4-HNA DEP (14a) in 1% aq MeOH released DEP with modest efficiency, Φ = 0.028. Similarly, the methoxy analog, 1,4-MNA DEP (14b), released DEP, but with a much lower efficiency (Φ = 0.0078). Both quantitatively produced DEP and, pleasingly, underwent photo-Favorskii rearrangements converting the aryl ketones to methyl naphthylacetates, blue-shifting the byproduct chromophore absorptions.

Both 14a and 14b now become the first new examples of photo-Favorskii rearrangements, here on a naphthyl platform. Previous examples are limited to phenacyl frameworks [6-9,15,38,39]. It is interesting to note, however, that replacement of the OH with OMe in the 1,4-HNA series results in both lower efficiency and decreased yield, a further analogy to that found with p-methoxy vs p-hydroxyphenacyl DEPs 4a,b (Table 4) [6,13,15,40] and with other leaving groups.

In contrast to the results in 1% aq MeOH, photolysis of 14a,b in anhydrous media or changing the co-solvent to MeCN resulted in a decrease in conversion (40–60%) and more complex reaction mixtures. The only products identified were DEP (quantitative) and reduction products 12 or 15, signaling a change in mechanism from heterolysis to homolysis. The low conversions are primarily due to competition for incident radiation by the unrearranged byproduct chromophore 12 or 15.

The role of H2O and hydroxylic solvents observed in this series is in complete accord with solvent effects on the parent pHP photoreactions [6,13,15,40-46]. The photo-Favorskii reaction (Scheme 7) is favored by aqueous-based solvents that serve both as a proton donor and an acceptor to the conjugate base generated from the chromophore’s triplet state heterolytic cleavage of the leaving group. The dual behavior of H2O is manifest in accepting a proton from the naphthol OH while simultaneously solvating the developing anionic charge on the departing nucleofuge. The resulting biradical 31, which must be a triplet by Wigner’s spin rule [47] is formed from the chromophore while on the excited state PES. Intersystem crossing (isc) to a diradicaloid ground state intermediate 32 (vide infra) is followed by cyclization to form the ‘Favorskii intermediate’ 33 [6,8,15,26,38,39,48].

[1860-5397-10-212-i7]

Scheme 7: Photo-Favorskii mechanism based on pHP DEP 4a photochemistry as applied to 1,4-HNA DEP (14a).

In contrast to the 1,4-naphthyl design, neither 10 nor 24 underwent Favorskii rearrangements. While both gave stoichiometric release of DEP, the chromophores either remain intact (for 10) as also reported for other 2-naphthylacetyl analogs [49] or are consumed by oxidation and fragmentation as in the case of 24. Thus, the conversion and chemical yields were low and the photolysates were complex mixtures of byproducts. In the case of 24, although the reaction efficiency was insensitive to O2 (implying singlet reactivity, not quenchable by ambient O2), the product mixture included several oxidation byproducts of the chromophore. Thus, photoreactions of 24 with ambient O2 were a complication not encountered with 10 or 14a,b.

The failure to deconjugate the carbonyl of either chromophore by rearrangement once again impinged on the overall conversion to released substrate. The formation of competing chromophoric byproducts limits the conversion and yields [6,10,11,15]. For example, changing the leaving group for 10, i.e., photolysis of 6-hydroxy-2-naphthylacetyl GABA ester (Figure 2B) inefficiently released 4-aminobutyrate (GABA) in low yield and is absent of any chromophore rearrangement.

It is surprising that 2,6-HNA DEP (10) and Wan’s 1,5-HNA acetate 27 [19] do not undergo a photo-Favorskii rearrangement. In the case of 27, Wan suggested that the lack of reactivity may have arisen from factors such as a lower excited state energy of the naphthyl ketone platform or the disruption of both aromatic rings for the 1,5 substituted analog 27 whereas only one ring is involved for the 1,4 substituted derivative [19].

Both of the contributing components of the 2,6 pattern, 2-naphthol (ET = 60.2 kcal/mol) and 2-acetylnaphthalene (ET = 59.5 kcal/mol) are higher energy contributors than the same two components of the 1,4 and 1,5 patterns, 1-naphthol (ET = 58.6 kcal/mol) and 1-acetylnaphthalene (ET = 56.4 kcal/mol) [10,11]. Of the two motifs, the 1,4 arrangement has the lower energy triplet. Yet the 2,6 pattern with the higher triplet energy is the less reactive and fails to participate in the photo-Favorskii rearrangement.

Wan’s work clearly demonstrates, however, that the excited state acidity of 1-naphthols is much greater than 2-naphthols and that excited-state intramolecular proton transfer (ESIPT) for 1-naphthols occurs at both the 5- and 8-positions by H–D exchange [19]. Furthermore, dehydration of 5-(1-hydroxyethyl)-1-naphthol (34) is very efficient, leading to quinone methide 35 which is trapped by solvent MeOH (Scheme 8). The corresponding 4-(1-hydroxyethyl)-1-naphthol, however, is unreactive. Comparisons of the two photoreactions, photodehydration and photo-Favorskii [48,50], and Wan’s results [19] for H–D exchange reactions demonstrate the importance of the relative location of the two functional groups and the role of ESIPT for both reactions. The hydroxy group, as Wan has elegantly determined, is the source of the acidic proton (especially for singlet state reactions). The carbonyl appendage, however, must be responsible for the enhanced heterolysis efficiency [28,29,38] and may influence the formation of the triplet excited state through intersystem crossing (isc) [26] which is the origin of the heterolysis and rearrangement processes for pHP.

[1860-5397-10-212-i8]

Scheme 8: Photodehydration and substitution of 5-(1-hydroxyethyl)-1-naphthol 34 [19].

Wan’s alternative suggestion that a disruption of both aromatic rings' π-conjugation would be less favorable than only one ring’s disruption has merit. For this to be a determining factor, the disruption would have to occur prior to or during the product determining formation of the ‘Favorskii’ intermediate 33 [38,48,50]. The putative key intermediates for 2,6-HNA and 1,5-HNA photo-Favorskii rearrangements (Scheme 9) illustrate the stage at which disruption in the π-network takes place. As in the case for the 1,4-HNA rearrangement, neither triplet biradical 337 nor 340 experience a change in connectivity within the aromatic nucleus. The formation of the triplet biradical is irreversible so that once generated, it must proceed on to a final product. Since no rearrangement products are formed for either the 2,6-HNA or 1,5-HNA, it is tempting to conclude that the decay to a ground state biradicaloid or zwitterion is the product determining step. In both cases, the aromaticity of both rings is lost upon formation of the Favorskii cyclopropanone intermediates 38 and 41.

[1860-5397-10-212-i9]

Scheme 9: Putative rearrangement intermediates for 1,5- and 2,6- HNA chromophores.

However, this rationale also falls short because the reactions of the similar biradicaloid or zwitterion are known to undergo nucleophilic substitution and not reduction to the methyl ketone products 6, 12 or 15 [38,39]. Substitution by solvent would trap the intermediate biradicaloid (or zwitterion) to form, in the case of 2,6-HNA, the α-methoxy ketone 42, which was not detected among the photoproducts.

The controlling feature leading to the change in mechanism for these two examples is more reasonably imbedded in the route to the reactive triplet excited state configuration [10,11]. For pHP, the reactive excited state is a π,π* triplet that is favored only in hydroxylic solvents, especially H2O [15,45,46,48]. In non-hydroxylic solvents, the (n,π*)3 dominates which leads to the classical α- and β-homolytic cleavage and H-abstraction reactions. For 2,6-HNA and 5,8-BQA, the reduction product, a methyl ketone, probably results from homolysis. The lower excited state acidity and the poor intersystem crossing in the 2,6-HNA platform and strong fluorescence of the 2,6-HNA chromophore (Figure 2) also diminishes formation of the triplet and, thus, its participation in a photo-Favorskii rearrangement. Finally, an indication of the photostability of the 2,6-HNA derivatives had earlier precedence in the lack of product formation for 2-hydroxy-6-trifluoromethylnaphthylene photo-dehydrofluorination reported by Seiler and Wirz [51]. For the 1,5-HNA acetate, the less reactive (higher pKa) of the leaving OAc group of 27 vs DEP, fluorescence decay and reversible ESIPT processes are major factors disfavoring photorelease.

A summary of the most frequently encountered examples of caged phosphates is given in Table 4. The data are reported for DEP leaving groups, when available, since DEP has proven to be a commonly employed test leaving group for PPG comparisons. It is generally found that the DEP model is reliable when extended to more complex phosphate leaving groups including nucleotides such as ATP and GTP and tyrosyl phosphates and thiophosphates [52-55]. The key comparisons for practical applications of photochemical deprotection are the maximum conversion which controls the chemical yield, the quantum yield (or photochemical efficiency) and the complexity of the photochemical products and byproducts in the photolyzate mixture. Solvents and excitation wavelengths are also given in Table 4 as a guide on selecting a PPG using these reaction parameters.

Table 4: A comparison of photorelease from cages for diethyl phosphates. Chemical yields, quantum yields, efficiencies and complexity based on solvents and chromophores.

PPG chromophore derivatives solventa λexc nm Φdis and (conversion
yield/%)
S1 or T1 chromo-
phore fateb
Ref
o-nitrobenzyl
[Graphic 1]
1a X=1 H2O 254, 308 N/Ac (28d)   mixture [56]
1b X=2 H2O 308, 355 N/A (90d) S1 N/A [56]
benzyl
[Graphic 2]
30a (R, X, Y = H) MeOH, n-BuOH,
t-BuOH
254 0.39 (50–60) S1 intact [35,57]
S(−)-30b (X, Y = H, R = Me) MeOH
t-BuOH
254 0.21–0.9e (77/28 rac) S1 intact [35,58]
30c (R, Y = H, X = OMe) n-BuOH, t-BuOH 254 0.42 (47) S1 intact [58]
30d (R, X = H, Y = OMe) t-BuOH 254 0.18 (28) S1 intact [58]
phenacyl
[Graphic 3]
4a (R = H, X = OH) MeOH 300 0.77 (87–96) T1 Favorskii [6,8,15,26,28,29]
4b (R = H, X = OMe) H2O, MeOH 300, 355 0.42f (27d/90f) S1 mixture [28,36,57]
4c (R = Me, X = H) MeOH 313 0.71 (94) nr intact [59]
benzoin
[Graphic 4]
2a (X = H) benzene 350 0.28 (72–79) T1 benzofuran [35,56]
2a (X = H) H2O/MeCN 350 0.37
(50d, 100)
T1 N/Ad, benzofuran [56,58,60]
2b (X = OMe) H2O 308, 350 N/A (55, 100)   benzofuran [8,56,61]
coumarin-4-ylmethyl
[Graphic 5]
3a
(X = OH)
MeOH 350 0.038 (<50) S1 intact [62,63]
3b
(X = OMe)
H2O/CH3CN, MeOH 333–350 0.04–0.08 S1 intact [58,60]
3c
(X = NMe2)
H2O 389 0.0041 (N/A) S1 intact [62,64]
naphthylmethyl 28 α MeOH 300 0.08 (N/A) S1 intact [35,58]
[Graphic 6] 29 β MeOH 300 0.3 (N/A) S1 intact [35,58]
naphthylacetyl
[Graphic 7]
10 (2,6)
(X = OH, R, Y = H)
1% aq MeCN 350 0.031 (67) T1 intact this work
14a (1,4)
(X, Y = H, R = OH)
1% aq MeOH 350 0.028 (98) T1 Favorskii this work
14b (1,4)
(X ,Y = H, R = OMe)
1% aq MeOH 350 0.0076 (40) T1 Favorskii this work
27 (1,5)
(X, R = H, Y = OH)
MeCN/H2O 300 nr (nrg)   intact [19]
hydroxyindolinyl
[Graphic 8]
24 MeCN/H2O 350 24 × 10−5 (30–44) S1 mixture this work

aThe ratio of H2O/MeCN was 1:1 unless stated otherwise. bWhen the chromophore is unchanged upon photolysis, fate of the chromophore is noted as “intact”. When the chromophore rearranges by the photo-Favorskii migration or closure to a benzofuran group, the fate is noted as Favorskii or benzofuran, respectively. When complex photoreactions occur with the chromophore, it is noted as “mixture”. cN/A = not available. dInorganic phosphate released in H2O ([56]), eWhen internal return is included. fIn MeOH, gnr = no reaction (leaving group was acetate).

Table 4 reveals that most chromophores either form complex mixtures of photoproducts complicating the isolation of pure, unprotected phosphates or they remain essentially unchanged (intact) and continue to absorb incident radiation, thus compromising the conversion to products and lengthening exposure of the sample to radiation. Three chromophores, benzoin, pHP, and now 1,4-hydroxynaphthylacetyl substrates cleanly undergo rearrangements that shift the absorption chromophore. Benzoins form benzofurans that, in fact, exacerbate the problem of light competition and fluorescence. Only pHP and 1,4-substituted naphthylacetyl 14 generate a less competitive chromophore allowing maximum conversion and quantitative chemical yield.

While pHP is highly efficient as a PPG (Φ’s approach 1.0) [15] the lower efficiency of 1,4-HNA (0.028) is competitive with many currently available PPGs. A measure often applied in biological decaging studies is the product of absorptivity and efficiency (εmax × Φ) which is 232 for photolysis of 1,4-HNA DEP at 325 nm, competitive with PPGs listed in Table 4. By comparison, although its absorptivity is very low at 325 nm, pHP DEP has a much larger εmax at 280 nm that gives a value of 6000 for excitation at 280 nm, an excitation wavelength that is biologically harmful [6,8,9].

Conclusion

We have designed and tested several new candidates for near-UV absorbing photoprotecting groups. 1,4-HNA DEP (14a) and 1,4-MNA DEP (14b) upon irradiation at 350 nm in 1% aq MeOH quantitatively release diethyl phosphate concomitant with a photo-Favorskii rearrangement with quantum yields of 0.028 for 1,4-HNA DEP and 0.0076 for 1,4-MNA DEP. The lower quantum efficiency of 1,4-MNA in MeOH is fully in accord with a similar methylation of the hydroxy group of pHP DEP, emphasizing the role of the phenolic OH on the reaction and rearrangement [6,15,46,48]. In anhydrous, non hydroxylic media, the mechanism of the reaction changes from heterolysis and reverts to the more traditional photochemically induced homolysis mechanism, leading to more complicated product mixtures and poor conversions.

The heterolysis mechanism of the photo-Favorskii rearrangement and release of DEP is initiated at the triplet state of 14 and proceeds according to the more extensively characterized rearrangement for pHP DEP. Due to a more favorable excitation wavelength of 350 nm for 1,4-HNA (vs 280 nm for pHP), it is anticipated that the 4-hydroxy-1-acylnaphthyl PPG will find more favorable applications as a PPG not only for phosphates but many other acidic functional groups. Additional studies on 2PE activation of 1,4-HNA DEP at 650 nm are also in progress.

2,6-HNA DEP and 5,8-BQA DEP also release DEP but do not undergo the beneficial aryl ketone deconjugative rearrangement, instead follow a homolytic pathway. This results in lower yields, poorer efficiencies, and incomplete conversion for 2,6-HNA, and intractable mixtures of chromophoric byproducts and secondary oxidation reactions with O2, for 5,8-BQA. Neither chromophore is suitable for application as a PPG.

Experimental

Synthesis

The details on the synthesis of all new compounds can be found in the Supporting Information File 1. The synthetic procedures and spectral data for all new compounds are reported in Supporting Information File 1.

Photochemistry

General methods

Photolyses were performed in a Rayonet RPR-100 photochemical reactor fitted with a merry-go-round apparatus and 16 × 350 nm (RPR 3500 Å) or 16 × 300 nm (RPR 3000 Å) lamps. The Rayonet reactor was turned on to warm up the lamps for 15 minutes prior to the irradiation. Samples were irradiated in NMR tubes or Pyrex test tubes at 40–45 °C in the presence of air unless indicated otherwise. The tubes were placed in a merry-go-round apparatus and the time of irradiation was recorded. Light output for the determination of quantum efficiencies was measured by using the potassium ferrioxalate method [65] under identical conditions used for the photolysis of the esters. Photolysis samples were analyzed for product formation and ester disappearance using 1H NMR and RP-HPLC methods. Dimethylformamide (DMF, 2 μL, 2.6 × 10−2 mmol) was used as an internal standard in 1H NMR analyses. For HPLC analyses the solvent was 70% CH3CN and 30% H2O unless otherwise noted. The detection wavelength varied depending on the ester analyzed as noted and the flow rate was 1.0 mL/min. Sample injections were performed in triplicate.

Photolysis of diethyl (2-(4-methoxynaphthalen-1-yl)-2-oxoethyl) phosphate (14b)

An NMR tube was charged with 14b (16 mg, 0.045 mmol) along with CD3OD (0.95 mL) and DMF (2 µL) as an internal standard. The sample was irradiated at 350 nm and 1H NMR scans were collected after 0, 30, and 60 min of photolysis. The depletion of 14b and the appearance of released diethyl phosphate were quantified from the integrations of the signals at δ 5.4 and 4.1 ppm, respectively. Results are shown in Table 1.

1. Quantum efficiency determination. A Pyrex tube was charged with 14b (6.2 mg, 0.018 mmol) in methanol (5 mL) and water (50 µL) and the contents were mixed thoroughly. The sample was irradiated at 350 nm and sample aliquots (150 µL) were taken after 0, 15, 30, and 45 min. The aliquots were analyzed by HPLC with a mobile phase of 90% acetonitrile and 10% water and a detection wavelength of 319 nm. The depletion of 14b was quantified from the peak areas of the signal at 3.5 min corresponding to the phosphate ester. The quantum efficiency was determined as described above. Results are shown in Table 1.

2. Photoproduct analysis in aqueous methanol. The photolysis mixture of 14b from the previous experiment was concentrated under reduced pressure and the residue washed with water and extracted with ethyl acetate. The organic extract was concentrated under vacuum and the residue analyzed with 1H NMR (CD3OD) and FAB–MS. The data for the major product matched that of an authentic sample of methyl (4-methoxy-1-naphthyl)acetate (26).

3. Photoproduct analysis in aqueous acetonitrile. Diethyl (2-(4-methoxynaphthalen-1-yl)-2-oxoethyl) phosphate (14b, 6 mg, 0.017 mmol) was dissolved in acetonitrile (5 mL) containing water (50 µL) in a Pyrex tube and irradiated at 350 nm with 9–3500 Å bulbs. Sample aliquots were taken after 0, 45, and 90 min of photolysis and analyzed by HPLC. The detection wavelength was 319 nm. The phosphate ester had a retention time of 5.6 min. A new peak emerged at 6.3 min that grew in intensity throughout the photolysis. Co-injection with an authentic sample of 1-acetyl-4-methoxynaphthalene (12) confirmed the ketone as a photoproduct from the reaction.

4. Diethyl (2-(4-methoxynaphthalen-1-yl)-2-oxoethyl) phosphate (14b): Dark reaction control experiment. An NMR tube was charged with 14b (ca. 5 mg), which was dissolved in methanol-d4 (<1 mL). The sample was heated to 46 °C in a warm water bath in the dark for 1 h. 1H NMR scans were taken before and after heating; no significant difference was observed in the NMR spectra. Also, 14a was shown to be stable for 30 h in 10% aq MeCN at rt in the dark.

Photolysis of diethyl (2-(4-hydroxynaphthalen-1-yl)-2-oxoethyl) phosphate (14a)

An NMR tube was charged with 14a (17 mg, 0.05 mmol) along with CD3OD (0.95 mL) and DMF (2 µL). The sample was irradiated at 350 nm and the percent conversion and deprotection % yield were determined as described above for 14b. Results are shown in Table 1.

1. Quantum efficiency determination in aqueous methanol. The same general procedure and HPLC conditions were employed as described for 14b. Amounts used: 14a (7.6 mg, 0.022 mmol), methanol (5 mL), water (50 µL). The detection wavelength was 323 nm. The phosphate ester had a retention time of 3.1 min. Results are shown in Table 1.

2. Quantum efficiency determination in aqueous acetonitrile. A Pyrex tube was charged with 14a (4.7 mg, 0.014 mmol), acetonitrile (5 mL) and water (50 µL). The sample was irradiated at 350 nm; aliquots (200 µL) were taken after 0, 2, 5, 7, and 10 min of photolysis and analyzed by HPLC on a C18 analytical column. The mobile phase contained 70% acetonitrile and 30% water, and the detection wavelength was 323 nm. The phosphate ester had a retention time of 3.5 min. The depletion of 14a was accompanied by the appearance of four new peaks at 1.7, 2.3, 2.6, and 3.9 min, which were not identified. Results are shown in Table 2.

3. Quantum efficiency determination in aqueous acetonitrile (degassed). A Pyrex tube was charged with 14a (5 mg, 0.015 mmol) along with acetonitrile (5 mL) and water (50 µL). The tube was fitted with a rubber septum and sparged with argon for 15 min. Sample aliquots (0.2 mL) were taken after 0, 2, 5, 7, and 10 min of photolysis using a needle syringe to avoid the introduction of air into the sample during irradiation. The aliquots were analyzed as described above. The percent conversion was 44% after 2 min of photolysis, thus the first two data points from a plot of mmol 14a vs time were used to estimate the slope of the regression line for depletion of 14a which led to an approximate value of 0.067 for the disappearance quantum efficiency (Table 2).

4. Triplet sensitization experiment. A quartz tube was charged with 14a (2.0 mg, 0.0059 mmol) and benzophenone (20 mg, 0.11 mmol), and the contents were dissolved in acetonitrile (10 mL) to which was added water (100 µL) to make a 1% aqueous solution. The solution was degassed with argon and irradiated with 6-RPR 2540 Å bulbs. Sample aliquots (0.2 mL) were taken after 0, 2, 5, 7, and 10 min of photolysis and analyzed by HPLC on a C18 analytical column with a mobile phase containing 70% acetonitrile and 30% water. The detection wavelength was 323 nm. The benzophenone had a retention time of 6.5 min and remained constant throughout the photolysis. The phosphate ester had a retention time of 3.6 min and its depletion was accompanied by the appearance of three new peaks at 1.6, 1.9, and 4.4 min, which were not assigned. The quantum efficiency was determined as described above. Results are shown in Table 2.

5. Photoproduct analysis in aqueous methanol. A Pyrex tube was charged with 14a (5 mg, 0.015 mmol), methanol (5 mL) and water (50 µL). The sample was irradiated at 350 nm for 15 min. After photolysis the solution changed from colorless to light yellow in appearance. The solvent was removed under reduced pressure and the remaining residue was dissolved in methylene chloride and washed with water. The organic extract was collected and the solvent concentrated to afford ca. 3 mg of an orange-colored residue. The residue was analyzed with 1H NMR in methanol-d4. Two signals were observed in the spectrum at δ 4.00 and 3.68 ppm, in a ratio of ca. 1:1.5, which were assigned to the methylene and methyl protons of methyl (4-hydroxy-1-naphthyl)acetate (25), based on the similar chemical shifts observed for methyl (4-methoxy-1-naphthyl)acetate (26).

6. Photoproduct analysis in aqueous acetonitrile. An NMR tube containing 14a (ca. 5 mg) was charged with CD3CN (1 mL) and D2O (0.1 mL). The solution was mixed thoroughly and irradiated at 350 nm for 30 min. The 1H NMR spectrum contained a singlet at δ 2.66 ppm that was assigned to the methyl protons of 1-acetyl-4-hydroxynaphthalene (15). The presence of 15 was confirmed upon spiking with an authentic sample.

Photolysis of diethyl (2-(6-hydroxynaphthalen-2-yl)-2-oxoethyl) phosphate (10)

Quantum efficiency determination. A Pyrex tube was charged with 10 (2.2 mg, 0.0065 mmol) along with acetonitrile (5 mL) and water (50 µL). The solution was irradiated at 350 nm and sample aliquots (200 µL) were taken after 0, 2, 5, 7, and 10 min of photolysis. The aliquots were analyzed by HPLC. The detection wavelength was 315 nm. The phosphate ester had a retention time of 3.4 min and its depletion was accompanied by the appearance of signals at 1.8 and 4.0 min. The quantum efficiency was determined as described above and displayed in Scheme 5dis= 0.031).

Photolysis of 4-[2-(6-hydroxy-2-naphthyl)-2-oxoethoxy]-4-oxobutan-1-aminium trifluoroacetate (2,6-HNA GABA)

Photoproduct analysis. A Pyrex tube was charged with 2,6-HNA GABA (19.5 mg, 0.068 mmol) along with acetonitrile (1.0 mL) and 50 mM TRIS buffer (9.0 mL, pH 7.3). The solution was photolyzed at 350 nm for 1 h, and sample aliquots of 50 µL were taken after 0, 30, and 60 min of photolysis. The aliquots were diluted with 150 µL of TRIS buffer and injected into an Econosphere C18 analytical column. The mobile phase contained 90% CH3CN with 0.1% TFA and 10% H2O. The flow rate was 1.0 mL/min and the detection wavelength was 244 nm. The depletion of 2,6-HNA GABA (retention time ca. 6 min) was accompanied by an increase in a peak with a retention time of ca. 3 min corresponding to the photoproduct(s). At the end of the photolysis, the solution contained an orange-colored precipitate. The organic soluble components were extracted with ethyl acetate, and the organic extract was washed with water, dried over magnesium sulfate, and the solvent removed under reduced pressure to afford ca. 6 mg of a yellow residue. The residue was dissolved in acetonitrile and injected into the HPLC column, resulting in a peak with a retention time of ca. 3 min. Co-injection of an authentic sample of 2-acetyl-6-hydroxynaphthalene (6) also produced the same peak. The residue was analyzed with 1H NMR (CDCl3) and found to contain the characteristic methyl singlet at δ 2.7 ppm corresponding to 6, in addition to other unassignable peaks further downfield in the spectrum. TLC analysis with 2:1 hexane/ethyl acetate further suggested the presence of 6 as a photoproduct from the reaction.

Fluorescence measurements. A solution of 2,6-HNA GABA (0.042 mM in pH 7.3 TRIS buffer containing 1% acetonitrile) was placed in a quartz cell in a Carey Eclipse fluorescence spectrometer. Measurements were made under ambient conditions.

Photolysis of 2-(8-(benzyloxy)quinolin-5-yl)-2-oxoethyl diethyl phosphate (24)

An NMR tube was charged with 24 (5.0 mg, 0.01 mmol) dissolved in CD3CN (900 μL), D2O (100 μL), and DMF (2 μL, 2.6 × 10−2 mmol). The sample was irradiated without degassing with 16 × 300 nm or 16 × 350 nm lamps and 1H NMR spectra (16 scans) were collected after 0, 30, 60, 90, and 120 min of photolysis. The depletion of 24 and the appearance of released diethyl phosphate were quantified from the integrations of the methylene signals at δ 5.30 ppm and 3.89 ppm, respectively.

1. Photolysis of 24 in the absence of oxygen. Using the same general procedure, the photolysis was repeated under degassed conditions. In these experiments, photolysis samples were purged with argon for 30 min before photolysis. The results are shown in Table 3.

2. Photoproduct analysis by 1H NMR. An NMR tube was charged with 24 (10 mg, 0.02 mmol) which was dissolved in CD3CN (900 μL) and D2O (100 μL). The contents were mixed thoroughly and the sample irradiated with air or under Ar purged conditions with 16 × 300 or 350 nm lamps. 1H NMR spectra were collected after 60 min of photolysis and used to calculate the percent conversion. The depletion of 24 and the appearance of released diethyl phosphoric acid were determined from the NMR signals for the methylene protons of the phosphate ester and methylene hydrogens of diethyl phosphoric acid at δ 5.30 ppm and 3.88 ppm, respectively. The photolysis sample was spiked with an authentic sample of diethyl phosphoric acid synthesized from diethyl chlorophosphate [66]. A dramatic increase in the intensities of the new methylene and methyl signals at 3.88 and 1.19 ppm, respectively, confirmed the presence of diethyl phosphate.

3. Control experiments with 24; stability in the absence of irradiation. An NMR tube was charged with 24 (10.0 mg, 0.02 mmol) along with 900 μL of CD3CN and 100 μL of D2O. The contents were mixed thoroughly and warmed in a water bath at 40 °C in the dark for 2 h. 1H NMR analysis before and after the heating showed no significant change in the NMR spectrum. No significant change was observed when the sample was re-examined 48 h later.

Supporting Information

Supporting Information File 1: Synthetic procedures and spectral data for all new compounds.
Format: PDF Size: 391.3 KB Download

Acknowledgements

Support of this work was provided by NIH grant R01 GM72910 (RSG) and by the University of Kansas.

References

  1. Havinga, E.; De Jongh, R. O. Bull. Soc. Chim. Belg. 1962, 71, 803–810. doi:10.1002/bscb.19620711131
    Return to citation in text: [1]
  2. Zimmerman, H. E.; Sandel, V. R. J. Am. Chem. Soc. 1963, 85, 915–922. doi:10.1021/ja00890a019
    Return to citation in text: [1]
  3. Zimmerman, H. E.; Somasekhara, S. J. Am. Chem. Soc. 1963, 85, 922–927. doi:10.1021/ja00890a020
    Return to citation in text: [1]
  4. Zimmerman, H. E. J. Am. Chem. Soc. 1995, 117, 8988–8991. doi:10.1021/ja00140a014
    Return to citation in text: [1]
  5. Buck, A. T.; Beck, C. L.; Winter, A. H. J. Am. Chem. Soc. 2014, 136, 8933–8940. doi:10.1021/ja501777r
    Return to citation in text: [1]
  6. Klán, P.; Šolomek, T.; Bochet, C. G.; Blanc, A.; Givens, R.; Rubina, M.; Popik, V.; Kostikov, V.; Wirz, J. Chem. Rev. 2013, 113, 119–191. doi:10.1021/cr300177k
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14]
  7. Bochet, C. G.; Blanc, A. In CRC Handbook of Organic Photochemistry and Photobiology, 3rd ed.; Griesbeck, A. G.; Oelgemöller, M.; Ghetti, F., Eds.; CRC Press: Boca Raton, Florida, 2012; pp 73–93.
    Return to citation in text: [1] [2] [3] [4]
  8. Goeldner, M.; Givens, R. S., Eds. Dynamic Studies in Biology; Wiley-VCH: Weinheim, Germany, 2005. doi:10.1002/3527605592
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
  9. Mayer, G.; Heckel, A. Angew. Chem., Int. Ed. 2006, 45, 4900–4921. doi:10.1002/anie.200600387
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7]
  10. Turro, N. J.; Ramamurthy, V.; Scaiano, J. C. Modern Molecular Photochemistry of Organic Molecules, 1st ed.; University Science Books: Sausalito, California, 2010.
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
  11. Klán, P.; Wirz, J. Photochemistry of Organic Compounds: From Concepts to Practice, 1st ed.; John Wiley & Sons: Chichester, 2009. doi:10.1002/9781444300017
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
  12. Mewes, J.-M.; Dreuw, A. Phys. Chem. Chem. Phys. 2013, 15, 6691–6698. doi:10.1039/c3cp44338h
    Return to citation in text: [1] [2] [3]
  13. Givens, R. S.; Kueper, L. W., III. Chem. Rev. 1993, 93, 55–66. doi:10.1021/cr00017a004
    Return to citation in text: [1] [2] [3] [4] [5]
  14. Pincock, J. A. In CRC Handbook of Organic Photochemistry and Photobiology, 2nd ed.; Horspool, W.; Lenci, F., Eds.; CRC Press: Boca Raton, Florida, 2010; Chapter 66.
    Return to citation in text: [1] [2]
  15. Givens, R. S.; Rubina, M.; Wirz, J. Photochem. Photobiol. Sci. 2012, 11, 472–488. doi:10.1039/c2pp05399c
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14]
  16. Engels, J.; Schlaeger, E.-J. J. Med. Chem. 1977, 20, 907–911. doi:10.1021/jm00217a008
    Return to citation in text: [1]
  17. Kaplan, J. H.; Forbush, B., III; Hoffman, J. F. Biochemistry 1978, 17, 1929–1935. doi:10.1021/bi00603a020
    Return to citation in text: [1]
  18. Givens, R. S.; Weber, J. F. W.; Jung, A. H.; Park, C.-H. In Methods in Enzymology; Marriott, G., Ed.; Academic Press: New York, 1998; Vol. 291, pp 1–29.
    Return to citation in text: [1]
  19. Lukeman, M.; Veale, D.; Wan, P.; Munasinghe, V. R. N.; Corrie, J. E. T. Can. J. Chem. 2004, 82, 240–253. doi:10.1139/v03-184
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7] [8] [9]
  20. Bauer, D. P.; Macomber, R. S. J. Org. Chem. 1975, 40, 1990–1992. doi:10.1021/jo00901a027
    Return to citation in text: [1]
  21. Arumugam, P.; Karthikeyan, G.; Perumal, P. T. Chromatographia 2004, 33, 1146–1147.
    Return to citation in text: [1]
  22. Zwierzak, A.; Kluba, M. Tetrahedron 1971, 27, 3163–3170. doi:10.1016/S0040-4020(01)98109-8
    Return to citation in text: [1]
  23. Matsumura, K. J. Am. Chem. Soc. 1930, 52, 4433–4436. doi:10.1021/ja01374a037
    Return to citation in text: [1]
  24. Iwakuma, T.; Tsunashima, A.; Ikezawa, K.; Takaiti, O. Novel carbostyril derivative and process for preparing same. Eur. Pat. Appl. EP0147719 A2, July 10, 1985.
    Return to citation in text: [1]
  25. Recuero, V.; de Gonzalo, G.; Brieva, R.; Gotor, V. Eur. J. Org. Chem. 2006, 18, 4224–4230. doi:10.1002/ejoc.200600385
    Return to citation in text: [1]
  26. Ma, C.; Kwok, W. M.; Chan, W. S.; Zuo, P.; Kan, J. T. W.; Toy, P. H.; Phillips, D. L. J. Am. Chem. Soc. 2005, 127, 1463–1472. doi:10.1021/ja0458524
    Return to citation in text: [1] [2] [3] [4]
  27. Gielen, H.; Alonso-Alija, C.; Hendrix, M.; Niewöhner, U.; Schauss, D. Tetrahedron Lett. 2002, 43, 419–421. doi:10.1016/S0040-4039(01)02162-1
    Return to citation in text: [1]
  28. Givens, R. S.; Park, C.-H. Tetrahedron Lett. 1996, 37, 6259–6262. doi:10.1016/0040-4039(96)01390-1
    Return to citation in text: [1] [2] [3] [4] [5]
  29. Park, C.-H.; Givens, R. S. J. Am. Chem. Soc. 1997, 119, 2453–2463. doi:10.1021/ja9635589
    Return to citation in text: [1] [2] [3] [4]
  30. Kötting, C.; Güldenhaupt, J.; Gerwert, K. Chem. Phys. 2012, 396, 72–83. doi:10.1016/j.chemphys.2011.08.007
    Return to citation in text: [1]
  31. Kötting, C.; Gerwert, K. Chem. Phys. 2004, 307, 227–232. doi:10.1016/j.chemphys.2004.06.051
    Return to citation in text: [1]
  32. Kötting, C.; Kallenbach, A.; Suveyzdis, Y.; Wittinghofer, A.; Gerwert, K. Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 6260–6265. doi:10.1073/pnas.0712095105
    Return to citation in text: [1]
  33. Kötting, C.; Kallenbach, A.; Suveyzdis, Y.; Eichholz, C.; Gerwert, K. ChemBioChem 2007, 8, 781–787. doi:10.1002/cbic.200600552
    Return to citation in text: [1]
  34. Du, X.; Frei, H.; Kim, S.-H. Biopolymers 2001, 62, 147–149. doi:10.1002/bip.1007
    Return to citation in text: [1]
  35. Givens, R. S.; Matuszewski, B. J. Am. Chem. Soc. 1984, 106, 6860–6861. doi:10.1021/ja00334a075
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7]
  36. Givens, R. S.; Athey, P. S.; Matuszewski, B.; Kueper, L. W., III; Xue, J.; Fister, T. J. Am. Chem. Soc. 1993, 115, 6001–6012. doi:10.1021/ja00067a015
    Return to citation in text: [1] [2]
  37. Khade, P. K.; Singh, A. K. Tetrahedron Lett. 2007, 48, 6920–6923. doi:10.1016/j.tetlet.2007.07.155
    Return to citation in text: [1]
  38. Šolomek, T.; Heger, D.; Ngoy, B. P.; Givens, R. S.; Klán, P. J. Am. Chem. Soc. 2013, 135, 15209–15215. doi:10.1021/ja407588p
    Return to citation in text: [1] [2] [3] [4] [5]
  39. Kammath, V. B.; Šolomek, T.; Ngoy, B. P.; Heger, D.; Klán, P.; Rubina, M.; Givens, R. S. J. Org. Chem. 2013, 78, 1718–1729. doi:10.1021/jo300850a
    Return to citation in text: [1] [2] [3]
  40. Epstein, W. W.; Garrossian, M. J. Chem. Soc., Chem. Commun. 1987, 532–533. doi:10.1039/c39870000532
    Return to citation in text: [1] [2]
  41. Ma, C.; Zuo, P.; Kwok, W. M.; Chan, W. S.; Kan, J. T. W.; Toy, P. H.; Phillips, D. L. J. Org. Chem. 2004, 69, 6641–6657. doi:10.1021/jo049331a
    Return to citation in text: [1]
  42. Chan, W. S.; Ma, C.; Kwok, W. M.; Phillips, D. L. J. Phys. Chem. A 2005, 109, 3454–3469. doi:10.1021/jp044546+
    Return to citation in text: [1]
  43. Ma, C.; Kwok, W. M.; Chan, W. S.; Du, Y.; Kan, J. T. W.; Toy, P. H.; Phillips, D. L. J. Am. Chem. Soc. 2006, 128, 2558–2570. doi:10.1021/ja0532032
    Return to citation in text: [1]
  44. Zuo, P.; Ma, C.; Kwok, W. M.; Chan, W. S.; Phillips, D. L. J. Org. Chem. 2005, 70, 8661–8675. doi:10.1021/jo050761q
    Return to citation in text: [1]
  45. Chen, X.; Ma, C.; Kwok, W. M.; Guan, X.; Du, Y.; Phillips, D. L. J. Phys. Chem. A 2006, 110, 12406–12413. doi:10.1021/jp064490e
    Return to citation in text: [1] [2]
  46. Phillips, D. L. In Hydrogen bonding and transfer in the excited state; Han, K.-L.; Zhao, G.-J., Eds.; John Wiley & Sons: Chichester, 2011; pp 287–312.
    Return to citation in text: [1] [2] [3]
  47. Calvert, J. G.; Pitts, J. N., Jr. Photochemistry; John Wiley & Sons: New York, 1966; p 88.
    Return to citation in text: [1]
  48. Givens, R. S.; Heger, D.; Hellrung, B.; Kamdzhilov, Y.; Mac, M.; Conrad, P. G., II; Cope, E.; Lee, J. I.; Mata-Segreda, J. F.; Schowen, R. L.; Wirz, J. J. Am. Chem. Soc. 2008, 130, 3307–3309. doi:10.1021/ja7109579
    Return to citation in text: [1] [2] [3] [4] [5]
  49. Suzuki, T.; Kaneko, Y.; Ikegami, M.; Arai, T. Bull. Chem. Soc. Jpn. 2004, 77, 801–806. doi:10.1246/bcsj.77.801
    Return to citation in text: [1]
  50. Rajesh, C. S.; Givens, R. S.; Wirz, J. J. Am. Chem. Soc. 2000, 122, 611–618. doi:10.1021/ja993070i
    Return to citation in text: [1] [2]
  51. Seiler, P.; Wirz, J. Tetrahedron Lett. 1971, 12, 1683–1686. doi:10.1016/S0040-4039(01)87434-7
    Return to citation in text: [1]
  52. Zou, K.; Miller, W. T.; Givens, R. S.; Bayley, H. Angew. Chem., Int. Ed. 2001, 40, 3049–3051. doi:10.1002/1521-3773(20010817)40:16<3049::AID-ANIE3049>3.0.CO;2-N
    Return to citation in text: [1]
  53. Kawakami, T.; Cheng, H.; Hashiro, S.; Nomura, Y.; Tsukiji, S.; Furuta, T.; Nagamune, T. ChemBioChem 2008, 9, 1583–1586. doi:10.1002/cbic.200800116
    Return to citation in text: [1]
  54. Aemissegger, A.; Carrigan, C. N.; Imperiali, B. Tetrahedron 2007, 63, 6185–6190. doi:10.1016/j.tet.2007.03.023
    Return to citation in text: [1]
  55. Rothman, D. M.; Shults, M. D.; Imperiali, B. Trends Cell Biol. 2005, 15, 502–510. doi:10.1016/j.tcb.2005.07.003
    Return to citation in text: [1]
  56. Baldwin, J. E.; McConnaughie, A. W.; Moloney, M. G.; Pratt, A. J.; Shin, S. B. Tetrahedron 1990, 46, 6879–6884. doi:10.1016/S0040-4020(01)87875-3
    Return to citation in text: [1] [2] [3] [4] [5] [6]
  57. Givens, R. S.; Athey, P. S.; Kueper, L. W., III; Matuszewski, B.; Xue, J. Y. J. Am. Chem. Soc. 1992, 114, 8708–8710. doi:10.1021/ja00048a059
    Return to citation in text: [1] [2]
  58. Givens, R. S.; Matuszewski, B.; Athey, P. S.; Stoner, M. R. J. Am. Chem. Soc. 1990, 112, 6016–6021. doi:10.1021/ja00172a016
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7]
  59. Klán, P.; Pelliccioli, A. P.; Pospišil, T.; Wirz, J. Photochem. Photobiol. Sci. 2002, 1, 920–923. doi:10.1039/B208171G
    Return to citation in text: [1]
  60. Schade, B.; Hagen, V.; Schmidt, R.; Herbrich, R.; Krause, E.; Eckardt, T.; Bendig, J. J. Org. Chem. 1999, 64, 9109–9117. doi:10.1021/jo9910233
    Return to citation in text: [1] [2]
  61. Corrie, J. E. T.; Katayama, Y.; Reid, G. P.; Anson, M.; Trentham, D. R.; Sweet, R. M.; Moffat, K. Philos. Trans. R. Soc., A 1992, 340, 233–244. doi:10.1098/rsta.1992.0063
    Return to citation in text: [1]
  62. Schmidt, R.; Geissler, D.; Hagen, V.; Bendig, J. J. Phys. Chem. A 2005, 109, 5000–5004. doi:10.1021/jp050581k
    Return to citation in text: [1] [2]
  63. Schmidt, R.; Geissler, D.; Hagen, V.; Bendig, J. J. Phys. Chem. A 2007, 111, 5768–5774. doi:10.1021/jp071521c
    Return to citation in text: [1]
  64. Pinheiro, A. V.; Parola, A. J.; Baptista, P. V.; Lima, J. C. J. Phys. Chem. A 2010, 114, 12795–12803. doi:10.1021/jp103045u
    Return to citation in text: [1]
  65. Hatchard, C. G.; Parker, C. A. Proc. R. Soc. London, Ser. A 1956, 235, 518–536. doi:10.1098/rspa.1956.0102
    Return to citation in text: [1]
  66. Schole, J.; Schole, C.; Eikemeyer, J.; Krebs, H. C. Tetrahedron 1994, 50, 1125–1128. doi:10.1016/S0040-4020(01)80823-1
    Return to citation in text: [1]
Other Beilstein-Institut Open Science Activities