Navigating and expanding the roadmap of natural product genome mining tools

• Friederike Biermann,
• Sebastian L. Wenski and
• Eric J. N. Helfrich

Beilstein J. Org. Chem. 2022, 18, 1656–1671, doi:10.3762/bjoc.18.178

• , terpene cyclases generate the oftentimes multicyclic, hydrocarbon scaffold via a carbocation-mediated cascade reaction [30]. Terpene cyclases are obligatory components of canonical terpene pathways and are used to identify terpene BGCs (Figure 3B) [30][31]. RiPPs, on the other hand, lack genes that are

• mining tools Some NP BGCs contain solely family-specific features, and lack universal class-specific signature sequences. In these cases, only members of the same subfamily can be identified via pHMMs. An example of the latter are RiPPs that are the most rapidly expanding NP subclass. Eighteen new RiPP

• are limited when it comes to solving complex problems [72]. An example of an advanced combination of different approaches and methods for the identification of RiPPs is the Data-driven Exploratory Class-independent RiPP TrackER (decRiPPter) [65]. decRiPPter uses a support vector machine algorithm

Posttranslational isoprenylation of tryptophan in bacteria

• Masahiro Okada,
• Tomotoshi Sugita and
• Ikuro Abe

Beilstein J. Org. Chem. 2017, 13, 338–346, doi:10.3762/bjoc.13.37

• (RIPPs), a conserved recognition motif in the N-terminal leader region of the precursor peptide enables the enzymatic modification of the C-terminal core peptide, and then the leader amino acids are frequently cleaved [2]. However, there is no obvious sequence within the N-terminal region of the ComX

• has occurred at an internal tryptophan residue of the precursor peptide. Kawaguchipeptin A Apart from the ComX pheromones, post-translational dimethylallylations of the tyrosine, threonine, serine, and tryptophan residues of cyclic peptides from cyanobacteria were reported [49][50][51]. The RiPPs

Cyclisation mechanisms in the biosynthesis of ribosomally synthesised and post-translationally modified peptides

• Andrew W. Truman

Beilstein J. Org. Chem. 2016, 12, 1250–1268, doi:10.3762/bjoc.12.120

• Andrew W. Truman Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK 10.3762/bjoc.12.120 Abstract Ribosomally synthesised and post-translationally modified peptides (RiPPs) are a large class of natural products that are remarkably chemically diverse given an

• intrinsic requirement to be assembled from proteinogenic amino acids. The vast chemical space occupied by RiPPs means that they possess a wide variety of biological activities, and the class includes antibiotics, co-factors, signalling molecules, anticancer and anti-HIV compounds, and toxins. A considerable

• heterocyclisation to form thiazolines or oxazolines, and radical-mediated reactions between unactivated carbons. Future prospects for RiPP pathway discovery and characterisation will also be highlighted. Keywords: biosynthesis; cyclisation; enzymes; peptides; RiPPs; Introduction Nature employs a number of routes