Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis

• Shijie Ye,
• Allison Ann Berger,
• Dominique Petzold,
• Oliver Reimann,
• Benjamin Matt and
• Beate Koksch

Beilstein J. Org. Chem. 2010, 6, No. 40, doi:10.3762/bjoc.6.40

• protein environment and to enable the design of fluorinated proteins with arbitrary desired properties. Keywords: chemical aminoacylation; DfeGly; fluorinated amino acids; site-specific protein mutagenesis; TfeGly; TfmAla; Introduction Over the past two decades, the interest in engineering proteins

• -pentenoyl chloride. We investigated the reaction both in pyridine and DMF as the solvent, in the case of DMF, 4-dimethylaminopyridine (DMAP) was added as base. Although, pyridine also behaves as a base (pKa 5.21), higher yields were achieved with DMAP in DMF. The synthesis of N-(4-pentenoyl)-TfmAla