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Search for "enrichment" in Full Text gives 70 result(s) in Beilstein Journal of Organic Chemistry.
Switchable molecular tweezers: design and applications
Beilstein J. Org. Chem. 2024, 20, 504–539, doi:10.3762/bjoc.20.45
- achieved by chemical (hydroxide/proton) or electrochemical (reduction/oxidation) stimuli allowing the enrichment of perylene from a mixture of polycyclic aromatic hydrocarbons (PAHs) in phase-transfer experiments into a perfluorocarbon phase. Metal cation responsive tweezers In the early 2000s, Lehn and co
Elucidating the glycan-binding specificity and structure of Cucumis melo agglutinin, a new R-type lectin
Beilstein J. Org. Chem. 2024, 20, 306–320, doi:10.3762/bjoc.20.31
- assigned binding motif is shown with added transparency. Full array data are available in Supporting Information File 1, tables “cfg” and “imperial”. (c) Enrichment analysis of glycan array data. For both NCFG and ICL array data, we used the get_pvals_motif function from glycowork [24] (version 0.8.1) with
Synthesis of ether lipids: natural compounds and analogues
Beilstein J. Org. Chem. 2023, 19, 1299–1369, doi:10.3762/bjoc.19.96
Non-peptide compounds from Kronopolites svenhedini (Verhoeff) and their antitumor and iNOS inhibitory activities
Beilstein J. Org. Chem. 2023, 19, 789–799, doi:10.3762/bjoc.19.59
- digestion solution, ground, and centrifuged to produce a cell suspension. Lymphocytes were isolated from the cell suspension using the Percoll method and were further enriched (via negative selection) to obtain naive CD8+ T cells. The enrichment effect and phenotype of the CD8+ cells were detected by flow
Chemistry of polyhalogenated nitrobutadienes, 17: Efficient synthesis of persubstituted chloroquinolinyl-1H-pyrazoles and evaluation of their antimalarial, anti-SARS-CoV-2, antibacterial, and cytotoxic activities
Beilstein J. Org. Chem. 2022, 18, 524–532, doi:10.3762/bjoc.18.54
- test systems, show EC50 values in the lower nM range [36][37][38][39]. Thus, the newly reported compounds are a valuable enrichment from the chemical perspective and have promising potential for further optimization or combination with other compounds. The structures of chloroquine, hydroxychloroquine
Enantioselective PCCP Brønsted acid-catalyzed aminalization of aldehydes
Beilstein J. Org. Chem. 2021, 17, 2433–2440, doi:10.3762/bjoc.17.160
- substituent on the aromatic ring was investigated. The position of bromine on the aromatic ring had a dramatic effect on the enantiomeric purity of the formed products 3k–n. When a bromine substituent is introduced in the “3” position of anthranilamide, the enantiomeric enrichment of aminal 3k reached only 30
Halides as versatile anions in asymmetric anion-binding organocatalysis
Beilstein J. Org. Chem. 2021, 17, 2270–2286, doi:10.3762/bjoc.17.145
- complex or active nucleophiles. In 2014, Jacobsen et al. developed a highly enantioselective selenocyclization reaction of olefins 26, using the chiral squaramide 28 as a dual hydrogen bond donor (Scheme 6) [16]. Although early-stage enantio-enrichment during the introduction of selenium is hard to
A systems-based framework to computationally describe putative transcription factors and signaling pathways regulating glycan biosynthesis
Beilstein J. Org. Chem. 2021, 17, 1712–1724, doi:10.3762/bjoc.17.119
- additional plots provided for additional cancer types in Supporting Information File 1 for luminal breast cancer. CREB3L4 and PRDM1 disproportionately affect the type I and II LacNAc pathway in luminal breast cancer: Our analysis suggests that CREB3L4 (enrichment p-value = 0.036) and PRDM1 (enrichment p
- directly impacted by TGF-β signaling, and thus increasing the metastatic potential of cancer [28]. MEF2C was found to be inhibited by MECP2 based on Reactome pathway enrichment. Since the glycosaminoglycan elongation pathways positively correlate to MEFC2 expression and MEFC2 is amplified in cancer, it is
- sulfate chain elongation: The MECP2 (enrichment p-value = 0.037) was found to positively regulate heparan sulfate elongation. MECP2 regulates gene expression by binding to methylated promoters and then by recruiting chromatin remodeling proteins to condense DNA and repress gene expression [29][30]. MECP2
Volatile emission and biosynthesis in endophytic fungi colonizing black poplar leaves
Beilstein J. Org. Chem. 2021, 17, 1698–1711, doi:10.3762/bjoc.17.118
- library was prepared, and PolyA enrichment was performed before sequencing on an IlluminaHiSeq 3000 sequencer (Max Planck Genome Centre, Cologne, Germany) with 25 Mio reads, 150 base pairs, paired end. Trimming of the obtained Illumina reads and de novo assembly were both performed with the program CLC
Chemical approaches to discover the full potential of peptide nucleic acids in biomedical applications
Beilstein J. Org. Chem. 2021, 17, 1641–1688, doi:10.3762/bjoc.17.116
Photoinduced post-modification of graphitic carbon nitride-embedded hydrogels: synthesis of 'hydrophobic hydrogels' and pore substructuring
Beilstein J. Org. Chem. 2021, 17, 1323–1334, doi:10.3762/bjoc.17.92
- ). After the polymerization was completed, polymer networks were purified as delineated in the experimental section. Altered pore morphology was investigated by SEM, and functional group analysis was achieved by FTIR. Enrichment of the hydrogel network with subsequent radical polymerization using various
Enhanced target cell specificity and uptake of lipid nanoparticles using RNA aptamers and peptides
Beilstein J. Org. Chem. 2021, 17, 891–907, doi:10.3762/bjoc.17.75
- exponential enrichment (SELEX) [30]. SELEX is an iterative process that begins with a large oligonucleotide library that, through a process of negative and positive selections, ends with a few candidates that are specific for a particular protein [30][31]. Using HIV-1 as our model, we explored the use of two
α,γ-Dioxygenated amides via tandem Brook rearrangement/radical oxygenation reactions and their application to syntheses of γ-lactams
Beilstein J. Org. Chem. 2021, 17, 688–704, doi:10.3762/bjoc.17.58
- did not improve the results, indicating that the reactions are at thermodynamic equilibrium after 24 h. Surprisingly, for N-methyl-substituted lactam 12e the diastereomeric enrichment remained rather moderate despite variations of temperature and time (Table 4, entry 5), whereas the diastereomeric
Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification
Beilstein J. Org. Chem. 2020, 16, 3038–3051, doi:10.3762/bjoc.16.253
- abundance of the individual proteins. For addressing these ambiguities, a more selective sample preparation is required, for example, using different enrichment strategies or proteases [23]. Interestingly, an additional allotype of the main IgG3 glycosylation site (EEQYNSTFR) was assigned in four out of
- Ultra system (Veolia Water Technologies Netherlands B.V., Ede, Netherlands). Sequencing-grade trypsin was obtained from Promega (Madison, WI). Sample preparation A detailed description of the methods for the immunoaffinity enrichment of the immunoglobulins and the glycopeptide preparation can be found
Dirhamnolipid ester – formation of reverse wormlike micelles in a binary (primerless) system
Beilstein J. Org. Chem. 2020, 16, 2820–2830, doi:10.3762/bjoc.16.232
- ingredients in a lot of technical applications. They can act as a flotation agent in the enrichment of ores [1], emulsifier and stabilizer for emulsions [2], or as additives for self-cleaning surfaces (artificial lotus effect) [3][4]. Rhamnolipids (RL, Figure 1) are biosurfactants, that are produced by
Synthesis of purines and adenines containing the hexafluoroisopropyl group
Beilstein J. Org. Chem. 2020, 16, 2739–2748, doi:10.3762/bjoc.16.224
- equivalent of elemental sulfur (Scheme 1). A crude mixture of 2a and 2b (ratio 70:30) was isolated in 78% yield. Since the minor isomer 2b had a significantly higher solubility in hexane, washing of the crude mixture with hexane resulted in an enrichment of the major isomer 2a (ratio 2a/2b 95:5 after washing
GlypNirO: An automated workflow for quantitative N- and O-linked glycoproteomic data analysis
Beilstein J. Org. Chem. 2020, 16, 2127–2135, doi:10.3762/bjoc.16.180
- proteomic sample preparation and protease digestion, coupled with depletion of abundant proteins or enrichment of glycopeptides to enable their measurement. There have also been several advances in glycopeptide quantification strategies including chemical labelling, label-free and data-independent
4-Hydroxy-3-methyl-2(1H)-quinolone, originally discovered from a Brassicaceae plant, produced by a soil bacterium of the genus Burkholderia sp.: determination of a preferred tautomer and antioxidant activity
Beilstein J. Org. Chem. 2020, 16, 1489–1494, doi:10.3762/bjoc.16.124
- providing sufficient evidence to support the structure, was isolated from a fermentation extract of Burkholderia sp. 3Y-MMP isolated from a soil by a Zn2+ enrichment culture. Detailed spectroscopic analyses by MS and NMR, combined with 13C chemical shift comparison with literature values of the related
- in the host organisms. Keywords: antioxidant; Burkholderia sp; quinolone; soil bacterium; Zn2+ enrichment culture; Findings 4-Hydroxy-2(1H)-quinolone (3) is a unique structural motif mostly found in alkaloids from rutaceous plants (family Rutaceae) [1][2]. This motif has several tautomeric forms
- secondary metabolism [15]. In the course of our continuing studies on bioactive metabolites of less studied bacterial taxa [16], Burkholderia sp. 3Y-MMP, isolated from soil by an exhaustive enrichment culture under Zn2+-load, was selected for a detailed chemical study, which resulted in the isolation of 4
Activated carbon as catalyst support: precursors, preparation, modification and characterization
Beilstein J. Org. Chem. 2020, 16, 1188–1202, doi:10.3762/bjoc.16.104
- of oxygen surface groups depending on the oxidizing agent. Oxidative treatment with liquid agents (HNO3 or H2O2) increases the amount of carboxylic groups, while carbonyl and hydroxy surface groups result from modification with O2 or N2O [110]. Nitrogen-containing surface groups: Nitrogen-enrichment
- different elemental compositions of the bituminous coal based materials after carbonization, activation and ammoxidation for the enrichment of nitrogen. He found that the carbonization step causes an increase of the carbon content, a decrease of oxygen and hydrogen and the nitrogen amount stays almost
- constant. The activation process leads to an increase of oxygen, while all other elements decrease. Thus, the activation reagent (KOH) oxidizes the initial material during this step. As expected, the ammoxidation results in an enrichment of nitrogen [10]. Scanning electron microscopy with energy-dispersive
A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA
Beilstein J. Org. Chem. 2020, 16, 1135–1141, doi:10.3762/bjoc.16.100
- random sequence molecules, also known as SELEX (systematic evolution of ligands by exponential enrichment), and was a holistic harpin [22]. In further work MGA was separated into two strands, and nucleic acid binding arms were added to each strand, allowing MGA to target a sequence of interest
[1,3]/[1,4]-Sulfur atom migration in β-hydroxyalkylphosphine sulfides
Beilstein J. Org. Chem. 2020, 16, 88–105, doi:10.3762/bjoc.16.11
- ]-rearrangement proceeded with additional partial enrichment of one enantiomer. The latter may have occurred through the preferential acylation of one of two diastereoisomers under the reaction conditions where the second chirality center at the phosphorus atom influenced the ratio of the acylation reaction. With
Isolation and biosynthesis of an unsaturated fatty acid with unusual methylation pattern from a coral-associated bacterium Microbulbifer sp.
Beilstein J. Org. Chem. 2019, 15, 2327–2332, doi:10.3762/bjoc.15.225
- ) system of bacterial groups. Therefore, the biosynthetic origin of 1 was investigated by feeding experiments of 13C-labeled precursors (Table 2). Firstly, in order to verify the origin of the carbon backbone, [1-13C]acetate was fed to the culture which gave a high enrichment of the carbons at C1, C3, C5
- , C7, and C9 (Figure 4). A feeding experiment with ʟ-[methyl-13C]methionine was then carried out. The high level of enrichment was observed only for C11 in the 13C NMR spectrum, thereby confirming that the methyl group is derived from methionine via SAM. In addition to the fungal sphingolipids
Cyclopropene derivatives of aminosugars for metabolic glycoengineering
Beilstein J. Org. Chem. 2019, 15, 584–601, doi:10.3762/bjoc.15.54
- cultivated with Ac4ManNCp, Ac4GlcNCp, Ac4GalNCp, or Ac4GlcNCyoc for 48 h. Subsequently, cells were harvested, lysed and the lysate was cleared by centrifugation resulting in an enrichment of soluble proteins. After labeling with Tz-Cy3 the proteins were separated by gel electrophoresis and blotted. Equal
Repurposing the anticancer drug cisplatin with the aim of developing novel Pseudomonas aeruginosa infection control agents
Beilstein J. Org. Chem. 2018, 14, 3059–3069, doi:10.3762/bjoc.14.284
- conducted for two biological replicates of each sample. The rRNA-depleted RNA was fragmented to 150–200 bp fragments, then first and second strand cDNA were synthesized with a cDNA-synthesis kit (ThermoScientific), followed by end repair and adapter ligation. After 12 cycles of PCR enrichment, the quality
- and control cells, using the heatmap.2 [19] package of R. Function enrichment analysis was conducted based on PseudoCAP Function Class (http://www.pseudomonas.com), and a dot plots figure was generated using the ggplot2 [20] package of R. The RNA-Seq datasets are available at the NCBI Sequence Read
- 1, Table S2) in P. aeruginosa. The heat-map and function enrichment of the genes that were differently expressed between cisplatin-treated and control P. aeruginosa samples were illustrated in Figure 2 and Figure 3, respectively. The cisplatin treatment triggered the expression of a large fraction
Lanyamycin, a macrolide antibiotic from Sorangium cellulosum, strain Soce 481 (Myxobacteria)
Beilstein J. Org. Chem. 2018, 14, 1554–1562, doi:10.3762/bjoc.14.132
- rotary evaporator giving 15.4 g of an oily crude extract. Enrichment of the target compounds was achieved by eliminating the highly polar and the lipophilic compounds by consecutive partitioning with ethyl acetate/water. The ethyl acetate phase was dried and partitioned further in methanol/n-heptane
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