TY - JOUR A1 - Tang, Xiaoqiong A1 - Zhang, Yan A1 - Mao, Jiangbing A1 - Wang, Yuhua A1 - Zhang, Zhenghong A1 - Wang, Zhengchao A1 - Yang, Hongqin T1 - Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy JF - Beilstein Journal of Nanotechnology PY - 2022/// VL - 13 SP - 560 EP - 569 SN - 2190-4286 DO - 10.3762/bjnano.13.47 PB - Beilstein-Institut JA - Beilstein J. Nanotechnol. UR - https://doi.org/10.3762/bjnano.13.47 KW - actin cytoskeleton KW - atomic force microscopy KW - migration KW - prostate cancer cells KW - substrate stiffness KW - viscoelasticity N2 - The stiffness of the extracellular matrix of tumour cells plays a key role in tumour cell metastasis. However, it is unclear how mechanical properties regulate the cellular response to the environmental matrix. In this study, atomic force microscopy (AFM) and laser confocal imaging were used to qualitatively evaluate the relationship between substrate stiffness and migration of prostate cancer (PCa) cells. Cells cultured on stiff substrates (35 kPa) undergone several interesting phenomena compared to those on soft substrates (3 kPa). Here, the stimulation generated by the stiff substrates triggered the F-actin skeleton to bundle its filaments, increasing the polarity index of the external contour of PCa cells. Analysis of AFM force–distance curves indicated that the elasticity of the cells cultured on 35 kPa substrates increased while the viscosity decreased. Wound-healing experiments showed that PCa cells cultured on 35 kPa substrates have higher migration potential. These phenomena suggested that the mechanical properties may be correlated with the migration of PCa cells. After actin depolymerisation, the elasticity of the PCa cells decreased while the viscosity increased, and the migration ability was correspondingly decreased. In conclusion, this study clearly demonstrated the relationship between substrate stiffness and the mechanical properties of cells in prostate tumour metastasis, providing a basis for understanding the changes in the biomechanical properties at a single-cell level. ER -