TY - JOUR A1 - Arriortua, Oihane K. A1 - Garaio, Eneko A1 - Herrero de la Parte, Borja A1 - Insausti, Maite A1 - Lezama, Luis A1 - Plazaola, Fernando A1 - García, Jose Angel A1 - Aizpurua, Jesús M. A1 - Sagartzazu, Maialen A1 - Irazola, Mireia A1 - Etxebarria, Nestor A1 - García-Alonso, Ignacio A1 - Saiz-López, Alberto A1 - Echevarria-Uraga, José Javier T1 - Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases JF - Beilstein Journal of Nanotechnology PY - 2016/// VL - 7 SP - 1532 EP - 1542 SN - 2190-4286 DO - 10.3762/bjnano.7.147 PB - Beilstein-Institut JA - Beilstein J. Nanotechnol. UR - https://doi.org/10.3762/bjnano.7.147 KW - magnetite nanoparticles KW - magnetic hyperthermia KW - RGD functionalization KW - tumor targeting N2 - This work reports important advances in the study of magnetic nanoparticles (MNPs) related to their application in different research fields such as magnetic hyperthermia. Nanotherapy based on targeted nanoparticles could become an attractive alternative to conventional oncologic treatments as it allows a local heating in tumoral surroundings without damage to healthy tissue. RGD-peptide-conjugated MNPs have been designed to specifically target αVβ3 receptor-expressing cancer cells, being bound the RGD peptides by “click chemistry” due to its selectivity and applicability. The thermal decomposition of iron metallo-organic precursors yield homogeneous Fe3O4 nanoparticles that have been properly functionalized with RGD peptides, and the preparation of magnetic fluids has been achieved. The nanoparticles were characterized by transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), electron magnetic resonance (EMR) spectroscopy and magnetic hyperthermia. The nanoparticles present superparamagnetic behavior with very high magnetization values, which yield hyperthermia values above 500 W/g for magnetic fluids. These fluids have been administrated to rats, but instead of injecting MNP fluid directly into liver tumors, intravascular administration of MNPs in animals with induced colorectal tumors has been performed. Afterwards the animals were exposed to an alternating magnetic field in order to achieve hyperthermia. The evolution of an in vivo model has been described, resulting in a significant reduction in tumor viability. ER -