TY - JOUR A1 - Pyle, Joseph R. A1 - Chen, Jixin T1 - Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging JF - Beilstein Journal of Nanotechnology PY - 2017/// VL - 8 SP - 2296 EP - 2306 SN - 2190-4286 DO - 10.3762/bjnano.8.229 PB - Beilstein-Institut JA - Beilstein J. Nanotechnol. UR - https://doi.org/10.3762/bjnano.8.229 KW - diffusion KW - PAINT KW - single-molecule photophysics KW - super-resolution imaging N2 - Super-resolution imaging of single DNA molecules via point accumulation for imaging in nanoscale topography (PAINT) has great potential to visualize fine DNA structures with nanometer resolution. In a typical PAINT video acquisition, dye molecules (YOYO-1) in solution sparsely bind to the target surfaces (DNA) whose locations can be mathematically determined by fitting their fluorescent point spread function. Many YOYO-1 molecules intercalate into DNA and remain there during imaging, and most of them have to be temporarily or permanently fluorescently bleached, often stochastically, to allow for the visualization of a few fluorescent events per DNA per frame of the video. Thus, controlling the fluorescence on–off rate is important in PAINT. In this paper, we study the photobleaching of YOYO-1 and its correlation with the quality of the PAINT images. At a low excitation laser power density, the photobleaching of YOYO-1 is too slow and a minimum required power density was identified, which can be theoretically predicted with the proposed method in this report. ER -