Novel acridone-modified MCM-41 type silica: Synthesis, characterization and fluorescence tuning

A Mobil Composition of Matter (MCM)-41 type mesoporous silica material containing N-propylacridone groups has been successfully prepared by co-condensation of an appropriate organic precursor with tetraethyl orthosilicate (TEOS) under alkaline sol–gel conditions. The resulting material was fully characterized by means of X-ray diffraction (XRD), N2-adsorption–desorption, transmission electron microscopy (TEM), IR and UV–vis spectroscopy, as well as 29Si and 13C CP-MAS NMR techniques. The material features a high inner surface area and a highly ordered two-dimensional hexagonal pore structure. The fluorescence properties of the organic chromophore can be tuned via complexation of its carbonyl group with scandium triflate, which makes the material a good candidate for solid state sensors and optics. The successful synthesis of highly ordered MCM materials through co-condensation was found to be dependent on the chemical interaction of the different precursors.


Introduction
Mesoporous silicates are widely used for a variety of applications such as gas storage and heterogeneous catalysis, e.g., the synthesis of ε-caprolactam [1], or the decomposition of nitrous oxides [2]. MCM-41, MCM-48 and other silica materials can normally be functionalized either by in situ post-sol-gel modification or by direct co-condensation of different types of organic Scheme 1: Synthesis of the sol-gel precursor 1.
precursors [3]. The latter method often leads to a more homogeneous distribution of the desired functionalization within the material. It also provides the possibility to characterize application-tailored sol-gel precursors prior to implementing them into the solid, thus increasing the depth of information compared to the data solely drawn from solid state measurements.
Focusing on the synthesis of novel inorganic-organic hybrid materials, we also investigated new ways to produce trialkoxysilanes bearing polycyclic aromatic compounds as terminal groups, Ar-(CH 2 ) n -Si(OR) 3 (n = 3, R = Me, Et), which may lead to interesting optical or electronic properties [4][5][6]. Acridone, being a well-known fluorophore used, e.g., for chemosensors [7], has previously been reported to be suitable for the pK a determination of lanthanide salts in aqueous solution, the fluorescence undergoing a bathochromic shift directly correlated to the acidity of the cation bound to its carbonyl group [8]. We therefore sought to create a micro-or mesoporous material containing covalently bound acridone units that would serve the same purpose, by choosing the amino function of acridone as the functional group to be modified. Trialkoxysilanes with a variety of functional groups have already been prepared by N-alkylation of amines using 3-iodopropyltrimethoxysilane (IPTMS) [9] or 3-bromopropyltrimethoxysilane (BPTMS) [10], by sulfamidation [11], by imide [12] or via imine forming reactions [13] using 3-aminopropyltriethoxysilane (APTES). In 2009, an N-alkylated acridone derivative bearing a (tri-isopropyloxysilyl)propyl group and its application as an anion-selective fluorescent probe were reported by Lin and Chen [14]. However, to the best of our knowledge, up to now, no MCM-like material featuring covalently bound acridone units has been described.

Results and Discussion
Compound 1 was prepared in a two-step sequence (Scheme 1): Following a published procedure, acridone was obtained from commercially available N-phenylanthranilic acid by an acid catalyzed ring closure reaction [15]. To attach the silyl functionalized linker, acridone was deprotonated by NaH and the resulting anion reacted with 3-iodopropyltrimethoxysilane (IPTMS).
Co-condensation of TEOS with organosilanes in the presence of an appropriate structure-directing template allows the direct and homogeneous incorporation of organic functionalities into a mesoporous material. Applying this method, we were recently able to introduce up to 30 wt % of redox-active phenothiazines into mesostructured silicas [4][5][6]. The resulting materials showed a continuous decrease of ordering with increasing bulk of the organic groups, although all exhibited very high specific surface areas. As the formation of micelles will strongly depend on the nature of the organic molecule and its concentration, it was decided to keep the amount of precursor 1 at 10 mol % with respect to TEOS in order to be sure that a highly ordered material would be obtained. The MCM-41 analogue was prepared by a modified synthesis previously reported by Pang et al. [15], in which aqueous ethylamine is used in order to adjust smoothly the pH-value of the solution. Given the fact that polycyclic compounds are rather bulky organic moieties which might behave differently when undergoing a sol-gel transformation, a slightly larger molecule (stearyltrimethylammonium bromide, C 18 TAB) was chosen as the templating agent.
However, obtaining materials with high surface areas has previously been shown to be also dependent on the boiling point of the sol-gel solution. Thus, the addition of larger amounts of volatile organic solvents such as THF to the aqueous phase often prevents the formation of highly ordered mesoporous structures [16]. On the other hand, the π-stacking of large aromatics generally causes them to be less readily dissolved and requires larger amounts of solvents, which are mostly immiscible with water. Therefore, pure TEOS was tested as a mediating agent for introduction into the sol-gel process. It turned out to be beneficial for the co-condensation process that precursor 1 could be mixed with TEOS rather easily, although it did not prove possible to obtain a homogeneous solution. Despite the fact that it was rather difficult to handle by syringe, a mixture of TEOS and 1 could be converted to the corresponding mesostructured silica MCM-ACR.
In contrast to 1, a similar compound prepared from pyrenesulfonyl chloride and APTES via sulfamidation immediately yielded a flocculent precipitate when brought in contact with TEOS. This observation might be explained by the combination of the pyrene moieties, forming strong π-bonding interactions, and the highly polar hydrogen bonding sulfonamide, causing the compound to become inhomogeneous in TEOS. As expected, the material obtained from the pyrene precursor showed a drastically decreased inner surface area of only 322 m 2 ·g −1 and no ordered material structure at all.
The CHNS analysis of MCM-ACR clearly indicates a slightly increased ratio between the dye and silica (1:7.5) compared to the initial ratio of the synthesis (1:9), probably resulting from different rates of hydrolysis of TEOS and the RSi(OMe) 3 groups. Converting the materials' molecular composition to the amount of N-propylacridone moieties per gram, we obtained a dye loading of approximately 240 mg·g −1 .
From the infrared spectra of 1 and both the unmodified as well as the modified MCM-ACR, the aromatic and aliphatic C-H vibrational bands around 3000 cm −1 showed reduced intensities in the solid and were mostly covered by the weak, yet broad, absorption bands of the silanol groups and bands from water trapped in the silica framework ( Figure 1). The spectra were also found to be in accordance with the interpretation of the infrared spectrum of free acridone reported earlier by Berezin et al. [17]. By comparing the region between = 1700 cm −1 and 1300 cm −1 , complex formation with the scandium(III) cation is clearly observed. The infrared absorption of the C=O vibrational band (precursor 1: 1630 cm −1 , N-methylacridone: 1630 cm −1 [18]) shifts slightly to lower wavenumbers ( Figure 2) after the immobilization, and also splits into two resonances (MCM-ACR: 1625 cm −1 , 1609 cm −1 ) indicating an interaction of the carbonyl group with Lewis or Brønsted acidic or with hydrogen-bond-donating surface sites, which has previously been described for fluorescent probes such as Michler's ketone [19]. This again changes after the reaction with Sc(OTf) 3 : A single C=O absorption emerges at 1613 cm −1 . The redshift of the absorption frequency is conclusive with respect to the weakened C=O double bond caused by the electron donation from the carbonyl unit to the scandium(III) cation, which is also evident in the UV-vis absorption of MCM-ACR (see below in Figure 9). According to the TEM analysis ( Figure 3) and BET measurements (Figure 4), the sol-gel process yielded a well ordered mesoporous material with a total surface area of up to 810 m 2 ·g −1 , exhibiting a characteristic pore size distribution with a sharp peak around 2.4 nm. The XRD spectrum reveals the expected peak patterns only for the (110) and (200) Miller indices as the (100) peak is not distinguishable from the primary beam ( Figure 5). BET data of an authentic MCM-41 sample previously prepared in our group corresponded to a total surface area of 1122 m 2 ·g −1 , so the experimental value for MCM-ACR is in accordance with our expectations. Table 1 summarizes the values obtained for the described MCM-41-sample as well as for both Sc(III)-free and Sc(III)-containing MCM-ACR.
The type IV BET isotherms of the material exhibit a large slope in the N 2 uptake only at lower p/p 0 ratios, and they show no significant sorption hysteresis. We may therefore conclude that the silica obtained has a very uniform structure and a homogeneous composition, thus proving that the co-condensation could be carried out in a controllable way, and that the formation of larger mesopores was prevented in the presence of the organic precursor in spite of its rather bulky nature.   The 13 C CP-MAS NMR spectrum of MCM-ACR ( Figure 6) shows the expected signals for the three methylene groups of the propyl chain as well as for the acridone moieties. Minor impurities in the spectrum of the precursor are mostly due to hydrolysis occurring during the measurement. The two prominent sharp alkyl peaks in the solid state spectrum may be attributed to the remaining free EtOH within the silicate. The 29 Si CP-MAS NMR data of MCM-ACR (Figure 7) prove that the material features the expected distribution of T-and Q-peaks. The minor T 2 -peak at −57 ppm can be ascribed to the R alkyl Si(OMe)(OSi) 2 unit resulting from the partially incomplete incorporation of the precursor's anchoring group into the framework [20].  When studying the fluorescence properties of the materials obtained before and after the post-sol-gel modification, an instantaneous shift in color and fluorescence was observed when MCM-ACR and the scandium(III) triflate solution were combined. Compound 1, as well as unmodified MCM-ACR, feature a clear blue fluorescence when excited at a wavelength of λ ex. = 366 nm, whereas the complex formation with the scandium cation results in an intensely yellow colored product showing a greenish fluorescence (Figure 8). This effect is also clearly evident in the UV-vis and fluorescence spectra ( Figure 9). Introduction of scandium(III) into the material yields a bathochromic effect in λ abs. and λ em. of about 20 nm and, more interestingly, a significantly increased absorption at around λ abs. = 328 nm. The appearance of this distinct absorption may hint towards an electron transfer (LMCT) from the molecular orbital located at the carbonyl oxygen to the empty 3d orbital residing at the Sc(III) cation, a process which would be similar to those that have been described for carboxylate complexes of Eu(III) [21]. The fluorescence spectrum of the modified silica shows a distinct shoulder at around λ em. ≈ 515 nm. This indicates a complex radiative relaxation of the formed transition metal species, the fluorescence originating from at least two different transitions between the excited electronic states and the ground state. A reason for this  phenomenon might be the energy splitting caused by the coupled CO stretching within the acridone-Sc 3+ complex [7]. The pure triflate is almost completely transparent in the UV-vis region and shows no fluorescence when measured both as a solid film and in solution, hence it is obvious that the optical properties of the material depend on the electronic structure of the acridone chromophore, the latter being significantly changed via formation of the complex as the scandium(III) ion strongly interacts with the oxygen atom of the C=O functional group. The complex itself seems to be very stable and inert to ligand exchange: Excessive washing with polar organic solvents and storing of an authentic sample for weeks, and even months, neither altered the appearance of the material nor decreased its fluorescence intensity.

Conclusion
We have presented a novel fluorescent organosilane bearing an acridone fluorophore and its successful transformation into a MCM-41 type material via co-condensation with TEOS. As predicted, the hybrid material shows a change in its fluorescence properties when non-covalently modified through scandium complex formation. The miscibility of the organic precursor or its concentrated solution with an excess of the major silicon source has been determined to be crucial for the synthesis of the hybrid material given herein. Possibilities to vary the dye content by using different molar ratios of the precursors in the sol-gel process are to be investigated in the near future. Furthermore, efforts will be made to elucidate the optical properties of the materials after doping with lanthanides or heavy metal cations, e.g. Eu(III), Er(III) or Bi(III).

Experimental
General: All starting materials described herein were purchased from Sigma-Aldrich and used as received. Solvents for the organic syntheses were dried prior to use according to standard procedures [22]. Solid-state 1 H CP-MAS, 29 Si CP-MAS and 13 C CP-MAS NMR spectra were recorded on a Bruker DSX Avance NMR spectrometer at resonance frequencies of 400 MHz, 101 MHz and 80 MHz for 1 H, 13 C or 29 Si nuclei, respectively. Liquid phase 1 H and 13 C NMR spectra were recorded on Bruker Spectrospin DPX-400 and Avance 600 devices at resonance frequencies of 400 MHz or 151 MHz for 1 H or 13 C nuclei, respectively. These spectra are internally referenced to SiMe 4 . The infrared spectra with a resolution of ±2 cm −1 were recorded using a PerkinElmer FT-ATR-IR 1000 spectrometer containing a diamond coated ZnSe-window. MALDI-ToF measurements were conducted on a Bruker Daltonics Ultraflex spectrometer. Elemental analyses were determined on a CHNS vario Microcube elemental analyzer (Elementar). X-ray powder diffraction (PXRD) patterns of the silica samples were recorded on a Siemens D5005 instrument using Ni-filtered Cu Kα radiation (λ = 1.5404 Å), with a step size of 1 °/min. N 2 -Adsorption-desorption isotherms, pore size distributions as well as the textural properties of the hybrid materials were determined at 77 K by a Quantachrome Autosorb 1 sorption analyzer. Before analysis, the samples were activated at 120 °C overnight in the vacuum and then the adsorption-desorption procedure was conducted by passing nitrogen into the sample, which was kept under liquid nitrogen. The average pore size of the samples was estimated using the BJH approach based on the Kelvin equation while assuming a cylindrically shaped porous structure. The specific surface areas were calculated by means of the Brunauer-Emmett-Teller (BET) equation in the low relative pressure interval (<0.3) and the pore size distribution curves were analyzed with the adsorption branch by the BJH method.  N-propylacridone moiety).
Post-sol-gel modification of MCM-ACR: Introduction of the scandium salt into the material was realized by stirring MCM-ACR (500 mg) in a 0.01 M solution of scandium(III) triflate in ethanol or acetonitrile (25 mL) for 16 h. The modified materials were thoroughly washed with ethanol or acetonitrile (5 × 5 mL) and water (5 × 5 mL) prior to drying and characterization.