TY - JOUR A1 - Sabek, Jad A1 - Díaz-Fernández, Francisco Javier A1 - Torrijos-Morán, Luis A1 - Díaz-Betancor, Zeneida A1 - Maquieira, Ángel A1 - Bañuls, María-José A1 - Pinilla-Cienfuegos, Elena A1 - García-Rupérez, Jaime T1 - Experimental study of an evanescent-field biosensor based on 1D photonic bandgap structures JF - Beilstein Journal of Nanotechnology PY - 2019/// VL - 10 SP - 967 EP - 974 SN - 2190-4286 DO - 10.3762/bjnano.10.97 PB - Beilstein-Institut JA - Beilstein J. Nanotechnol. UR - https://doi.org/10.3762/bjnano.10.97 KW - evanescent field KW - half-antibodies KW - light-assisted immobilization KW - photonic bandgap sensor KW - SNOM characterization N2 - A photonic bandgap (PBG) biosensor has been developed for the label-free detection of proteins. As the sensing in this type of structures is governed by the interaction between the evanescent field going into the cladding and the target analytes, scanning near-field optical microscopy has been used to characterize the profile of that evanescent field. The study confirms the strong exponential decrease of the signal as it goes into the cladding. This means that biorecognition events must occur as close to the PBG structure surface as possible in order to obtain the maximum sensing response. Within this context, the PBG biosensor has been biofunctionalized with half-antibodies specific to bovine serum albumin (BSA) using a UV-induced immobilization procedure. The use of half-antibodies allows one to reduce the thickness of the biorecognition volume down to ca. 2.5 nm, thus leading to a higher interaction with the evanescent field, as well as a proper orientation of their binding sites towards the target sample. 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