TY - JOUR A1 - Oliveira, Joana S. L. A1 - Brezesinski, Gerald A1 - Hill, Alexandra A1 - Gericke, Arne T1 - Influence of calcium on ceramide-1-phosphate monolayers JF - Beilstein Journal of Nanotechnology PY - 2016/// VL - 7 SP - 236 EP - 245 SN - 2190-4286 DO - 10.3762/bjnano.7.22 PB - Beilstein-Institut JA - Beilstein J. Nanotechnol. UR - https://doi.org/10.3762/bjnano.7.22 KW - calcium KW - ceramide-1-phosphate KW - Langmuir monolayers KW - phase behaviour KW - structural properties N2 - Ceramide-1-phosphate (C1P) plays an important role in several biological processes, being identified as a key regulator of many protein functions. For instance, it acts as a mediator of inflammatory responses. The mediation of the inflammation process happens due to the interaction of C1P with the C2 domain of cPLA2α, an effector protein that needs the presence of submicromolar concentrations of calcium ions. The aim of this study was to determine the phase behaviour and structural properties of C1P in the presence and absence of millimolar quantities of calcium in a well-defined pH environment. For that purpose, we used monomolecular films of C1P at the soft air/liquid interface with calcium ions in the subphase. The pH was varied to change the protonation degree of the C1P head group. We used surface pressure versus molecular area isotherms coupled with other monolayer techniques as Brewster angle microscopy (BAM), infrared reflection–absorption spectroscopy (IRRAS) and grazing incidence X-ray diffraction (GIXD). The isotherms indicate that C1P monolayers are in a condensed state in the presence of calcium ions, regardless of the pH. At higher pH without calcium ions, the monolayer is in a liquid-expanded state due to repulsion between the negatively charged phosphate groups of the C1P molecules. When divalent calcium ions are added, they are able to bridge the highly charged phosphate groups, enhancing the regular arrangement of the head groups. Similar solidification of the monolayer structure can be seen in the presence of a 150 times larger concentration of monovalent sodium ions. Therefore, calcium ions have clearly a strong affinity for the phosphomonoester of C1P. ER - TY - JOUR AU - Kolesnick, R. N. AU - Hemer, M. R. JF - J. Biol. Chem. PY - 1990/// VL - 265 SP - 18803 EP - 18808 ER - TY - JOUR AU - Pettus, B. J. AU - Bielawska, A. AU - Subramanian, P. AU - Wijesinghe, D. S. AU - Maceyka, M. AU - Leslie, C. C. AU - Evans, J. H. AU - Freiberg, J. AU - Roddy, P. AU - Hannun, Y. A. AU - Chalfant, C. E. JF - J. Biol. Chem. PY - 2004/// VL - 279 SP - 11320 EP - 11326 DO - 10.1074/jbc.M309262200 ER - TY - JOUR AU - Maceyka, M. AU - Spiegel, S. JF - Nature PY - 2014/// VL - 510 SP - 58 EP - 67 DO - 10.1038/nature13475 ER - TY - JOUR AU - Arana, L. AU - Gangoiti, P. AU - Ouro, A. AU - Trueba, M. AU - Gómez-Muñoz, A. JF - Lipids Health Dis. PY - 2010/// VL - 9 SP - 1 EP - 12 DO - 10.1186/1476-511X-9-15 ER - TY - JOUR AU - Chalfant, C. E. AU - Spiegel, S. JF - J. Cell Sci. PY - 2005/// VL - 118 SP - 4605 EP - 4612 DO - 10.1242/jcs.02637 ER - TY - JOUR AU - Subramanian, P. AU - Stahelin, R. V. AU - Szulc, Z. AU - Bielawska, A. AU - Cho, W. AU - Chalfant, C. E. JF - J. Biol. Chem. PY - 2005/// VL - 280 SP - 17601 EP - 17607 DO - 10.1074/jbc.M414173200 ER - TY - JOUR AU - Pettus, B. J. AU - Bielawska, A. AU - Spiegel, S. AU - Roddy, P. AU - Hannun, Y. A. AU - Chalfant, C. E. JF - J. Biol. Chem. PY - 2003/// VL - 278 SP - 38206 EP - 38213 DO - 10.1074/jbc.M304816200 ER - TY - JOUR AU - Kooijman, E. E. AU - Vaknin, D. AU - Bu, W. AU - Joshi, L. AU - Kang, S.-W. AU - Gericke, A. AU - Mann, E. K. AU - Kumar, S. JF - Biophys. J. PY - 2009/// VL - 96 SP - 2204 EP - 2215 DO - 10.1016/j.bpj.2008.11.062 ER - TY - JOUR AU - Kooijman, E. E. AU - Sot, J. AU - Montes, L.-R. AU - Alonso, A. AU - Gericke, A. AU - de Kruijff, B. AU - Kumar, S. AU - Goñi, F. M. JF - Biophys. J. PY - 2008/// VL - 94 SP - 4320 EP - 4330 DO - 10.1529/biophysj.107.121046 ER - TY - JOUR AU - Kooijman, E. E. AU - Burger, K. N. J. JF - Biochim. Biophys. Acta, Mol. Cell Biol. Lipids PY - 2009/// VL - 1791 SP - 881 EP - 888 DO - 10.1016/j.bbalip.2009.04.001 ER - TY - JOUR AU - Brezesinski, G. AU - Möhwald, H. JF - Adv. Colloid Interface Sci. PY - 2003/// VL - 100–102 SP - 563 EP - 584 DO - 10.1016/S0001-8686(02)00071-4 ER - TY - JOUR AU - Mendelsohn, R. AU - Moore, D. J. JF - Chem. Phys. Lipids PY - 1998/// VL - 96 SP - 141 EP - 157 DO - 10.1016/S0009-3084(98)00085-1 ER - TY - JOUR AU - Kooijman, E. E. AU - Carter, K. M. AU - van Laar, E. G. AU - Chupin, V. AU - Burger, K. N. J. AU - de Kruijff, B. JF - Biochemistry PY - 2005/// VL - 44 SP - 17007 EP - 17015 DO - 10.1021/bi0518794 ER - TY - JOUR AU - Laroche, G. AU - Dufourc, E. J. AU - Dufourcq, J. AU - Pézolet, M. JF - Biochemistry PY - 1991/// VL - 30 SP - 3105 EP - 3114 DO - 10.1021/bi00226a018 ER - TY - JOUR AU - Casares, J. J. G. AU - Camacho, L. AU - Martín-Romero, M. T. AU - Cascales, J. J. L. JF - ChemPhysChem PY - 2008/// VL - 9 SP - 2538 EP - 2543 DO - 10.1002/cphc.200800321 ER - TY - JOUR AU - Kjaer, K. JF - Physica B PY - 1994/// VL - 198 SP - 100 EP - 109 DO - 10.1016/0921-4526(94)90137-6 ER - TY - JOUR AU - Kaganer, V. M. AU - Möhwald, H. AU - Dutta, P. JF - Rev. Mod. Phys. PY - 1999/// VL - 71 SP - 779 EP - 819 DO - 10.1103/RevModPhys.71.779 ER - TY - JOUR AU - Stefaniu, C. AU - Brezesinski, G. JF - Curr. Opin. Colloid Interface Sci. PY - 2014/// VL - 19 SP - 216 EP - 227 DO - 10.1016/j.cocis.2014.01.004 ER - TY - JOUR AU - Bringezu, F. AU - Dobner, B. AU - Brezesinski, G. JF - Chem. – Eur. J. PY - 2002/// VL - 8 SP - 3203 EP - 3210 DO - 10.1002/1521-3765(20020715)8:14<3203::AID-CHEM3203>3.0.CO;2-6 ER - TY - JOUR AU - Antipina, M. N. AU - Schulze, I. AU - Dobner, B. AU - Langner, A. AU - Brezesinski, G. JF - Langmuir PY - 2007/// VL - 23 SP - 3919 EP - 3926 DO - 10.1021/la062840i ER - TY - JOUR AU - Wijesinghe, D. S. AU - Subramanian, P. AU - Lamour, N. F. AU - Gentile, L. B. AU - Granado, M. H. AU - Bielawska, A. AU - Szulc, Z. AU - Gomez-Munos, A. AU - Chalfant, C. E. JF - J. Lipid Res. PY - 2009/// VL - 50 SP - 1986 EP - 1995 DO - 10.1194/jlr.M800367-JLR200 ER - TY - JOUR AU - Li Destri, G. AU - Malfatti Gasperini, A. A. AU - Konovalov, O. JF - Langmuir PY - 2015/// VL - 31 SP - 8856 EP - 8864 DO - 10.1021/acs.langmuir.5b02054 ER - TY - JOUR AU - Smilgies, D.-M. AU - Boudet, N. AU - Struth, B. AU - Konovalov, O. JF - J. Synchrotron Radiat. PY - 2005/// VL - 12 SP - 329 EP - 339 DO - 10.1107/S0909049505000361 ER -