TY - JOUR A1 - Kishore Prasad, Pragati A1 - Lahav-Mankovski, Naama A1 - Motiei, Leila A1 - Margulies, David T1 - Encrypting messages with artificial bacterial receptors JF - Beilstein Journal of Organic Chemistry PY - 2020/// VL - 16 SP - 2749 EP - 2756 SN - 1860-5397 DO - 10.3762/bjoc.16.225 PB - Beilstein-Institut JA - Beilstein J. Org. Chem. UR - https://doi.org/10.3762/bjoc.16.225 KW - artificial receptors KW - cell surface modification KW - fluorescent probes KW - molecular cryptography N2 - A method for encrypting messages using engineered bacteria and different fluorescently labeled synthetic receptors is described. We show that the binding of DNA-based artificial receptors to E. coli expressing His-tagged outer membrane protein C (His-OmpC) induces a Förster resonance energy transfer (FRET) between the dyes, which results in the generation of a unique fluorescence fingerprint. Because the bacteria continuously divide, the emission pattern generated by the modified bacteria dynamically changes, enabling the system to produce encryption keys that change with time. Thus, this development indicates the potential contribution of live-cell-based encryption systems to the emerging area of information protection at the molecular level. ER -