A chiral analog of the bicyclic guanidine TBD: synthesis, structure and Brønsted base catalysis

Starting from (S)-β-phenylalanine, easily accessible by lipase-catalyzed kinetic resolution, a chiral triamine was assembled by a reductive amination and finally cyclized to form the title compound 10. In the crystals of the guanidinium benzoate salt the six membered rings of 10 adopt conformations close to an envelope with the phenyl substituents in pseudo-axial positions. The unprotonated guanidine 10 catalyzes Diels–Alder reactions of anthrones and maleimides (25–30% ee). It also promotes as a strong Brønsted base the retro-aldol reaction of some cycloadducts with kinetic resolution of the enantiomers. In three cases, the retro-aldol products (48–83% ee) could be recrystallized to high enantiopurity (≥95% ee). The absolute configuration of several compounds is supported by anomalous X-ray diffraction and by chemical correlation.


Introduction
In guanidinium ions charge delocalization is an important factor to stabilize the protonated form. As a result, guanidines are exceptionally strong nitrogen bases. As part of the amino acid arginine, they play an important role in biochemistry, mainly by forming ion pairs. In addition, numerous guanidine derivatives with complex cyclic structures can be found in natural products [1]. Simple guanidines such as tetramethylguanidine have been used as strong Brønsted bases in countless applications [2,3].
Compared to guanidine 7 ring expansion into structure 10 would shift the stereogenic phenyl groups into closer proximity to a hydrogen-bonded guest molecule and thereby might improve the enantioselective control exerted by 10 when used as a catalyst. In this article we describe the synthesis of guanidine 10 together with some initial applications as a chiral Brønsted base.

Results and Discussion
Previous syntheses of bicylic guanidines 2 and 6-9 started from enantiomerically pure α-amino acids [6,8,10,16,17] or from their reduction products, chiral α-amino alcohols [9,18]. In contrast, our approach used the racemic ester rac-12 of β-phenylalanine, easily accessible in a Knoevenagel-type condensation of benzaldehyde 11, ammonium acetate and malonic acid, followed by esterification (Scheme 1) [20,21]. A kinetic resolution of the enantiomers was achieved by enzymatic hydrolysis with Amano lipase PS from Burkholderia cepacia [22,23], a method already optimized for technical use [24]. The best results were obtained with methyl tert-butyl ether as a cosolvent [24]. By simple precipitation, batches larger than 15 g of the S-configurated acid 13 could be isolated in 90% yield (45% based on rac-12). In a two-step procedure 13 was converted into amino alcohol 14 without recrystallization in order to keep the enantiomeric excess unchanged. It was determined at this stage to be better than 99%. The S-configuration was assigned to 13 in accord with published data [22][23][24]. Intermediate 14 was converted into amine 16 by mesylation (79%), reaction with NaN 3 (96%) and hydrogenation (84%). Aldehyde 17, also accessible from 14 by oxidation (87%), then could be coupled with amine 16 in a reductive amination to form 18 (58%). After removal of the Boc protecting group (quant.), triamine 19 was reacted with dimethyl trithiocarbonate in refluxing nitromethane. The thiourea intermediate was activated in situ by S-alkylation with MeI. Upon further heating the final cyclization occured forming the iodide salt 10a of S,Sconfigurated guanidine 10 (67%). The free base could be isolated by extraction with CH 2 Cl 2 from 20 M aqueous NaOH (95%). For experimental details see Supporting Information File 1.
A crystal structure could be obtained from the benzoate salt of 10 ( Figure 2). The asymmetric unit contains two cations, two benzoate anions and ethyl acetate as a solvate molecule. Each cation is connected by two N-H···O hydrogen bonds to benzoate ions. The rings of cation 10 adopt a conformation close to an envelope with the phenyl substituents in pseudoaxial positions. The ion pairs and the solvate molecule are also connected by a number of very weak intermolecular C-H···π (phenyl) and C-H···O contacts (see Supporting Information File 2).
The chiral guanidine 10 was tested as a Brønsted base catalyst to promote the reaction of anthrones 20 or 21 with maleimides 22-24 (Scheme 2) [19,[25][26][27][28]. Depending on the substituents and the strength of the Brønsted base either Diels-Alder adducts or Michael products may be formed. Weaker bases such as ion-free bisoxazolines [28] or triethylamine are known to induce Diels-Alder adducts selectively whereas strongly basic guanidines such as 8 may also form the Michael products [19] which are the dominant products in polar solvents [25]. Previous mechanistic studies by Koerner and Rickborn [25] have collected strong arguments for a fast concerted [4 + 2]-cycloaddition of the deprotonated anthrone. Michael products were shown to be secondary products of a base-catalyzed retro-aldol reaction and they are not converted backwards into Diels-Alder adducts under such conditions [19,25,26]. Our results shown below give further support to this view. Riant, Kagan and Ricard have demonstrated for the first time that deprotonated anthrones may coordinate to chiral counterions in less polar solvents. Up to 61% ee could be obtained using cinchona alkaloids as catalysts [26]. The subsequent work of Tan and co-workers with guanidine catalyst 8 achieved enantioselectivities as high as 99% ee [19]. In recent years functionalized chiral amines have been successfully used as catalysts for anthrone maleimide cycloadditions [29][30][31][32][33][34][35]. In the presence of To determine the enantioselectivity of guanidine 10 we started with the structurally simplest case, the reaction of anthrone 20 and N-phenylmaleimide (22) which finally turned out to be the most complex one ( Table 1). The best but still low ee values for cycloadduct 25 were obtained in CHCl 3 (37% ee) and CH 2 Cl 2 (34% ee). Much better enantioselectivities of the Michael prod- uct 28 were found for reactions in solvents of increased polarity. Assuming that 28 is formed from 25 in a retro-aldol step, one might expect similar ee values for both compounds. This is clearly not the case. In THF the 83% ee for compound 28 strongly deviates from the low value for 25 (6% ee). Obviously, in the reaction of 20 and 22, a Diels-Alder step with low to moderate stereoselectivity is superimposed with a kinetic resolution of enantiomers in the subsequent retro-aldol reaction. Both are catalyzed by the chiral guanidine 10. As a result, the numbers shown in Table 1 are not constant but depend on the relative turnover of each reaction.
When rac-25 reacted with 0.1 equiv of 10 in THF at −15 °C, at 22% conversion ee values of 18% and 70% were found for compounds 25 and 28. The dominant isomer of Michael product 28 is shown below to be R configurated which corresponds to the slower running peak in Figure 3B. The R isomer of 28 is formed from the S,S isomer of 25 with retention of configuration (Scheme 2). The change from S to R is caused by a change in the CIP priorities of the substituents. Thus the faster running smaller peak in Figure 3A must correspond to the S,S enantiomer of 25. Assuming independent first order rate laws for the opening of the S,S and R,R enantiomers of 25, the best numerical fit is obtained for k S,S /k R,R ≈ 6.5. When both steps, Diels-Alder and Michael reaction, are catalyzed by guanidine 10 in THF (see Table 1), at 45% conversion the R isomer of 28 and the R,R isomer of 25 are the dominant species. However, due to the faster reaction of S,S-25 the numerical simulation shows that not the R,R but the S,S isomer is the preferred product of the Diels-Alder step with approximately 33% ee. This value comes close to the numbers obtained for the production of 26 (25% ee; 30% ee in THF) and 27 (28% ee) in CH 2 Cl 2 when no secondary conversion occurs. In contrast, when compound 21 reacts with 24 ring opening is so fast that no Diels-Alder product accumulates. Here the ee value of the Michael product 31 (21%) must correspond to the stereoselectivity of the Diels-Alder step. Due to low stereoselectivities and fast retroaldol reactions guanidine 10 is not optimal for the preparation of Diels-Alder adducts. We therefore focussed our efforts on the Michael products: Anthrone 20 and maleimide 22 reacted with 0.  [28] with bisoxazoline catalyst 33, we have assigned the S,S configuration to the major isomer of product 32. By hydrogenolysis, we could now convert such a sample (40% ee) into 25. The product S,S-25 was formed as the dominant product (35% ee) thus confirming our previous assignment. Under mild basic conditions, S,S-32 finally could be opened into R configurated Michael product 30 [25,26] (31% ee) to generate a stereochemically consistent view.

Conclusion
(S)-β-Phenylalanine (13), readily accessible by enantioselective hydrolysis of ester rac-12, is the starting material for an efficient synthesis of the chiral TBD analog 10. The cyclization steps in particular, which convert the triamine 19 into the final guanidine 10, give considerably higher yields compared to guanidines with five-membered rings such as compounds 6-9.
Apart from the better synthetic accessibility the formal ring expansion to the six-membered structure of 10 does not increase the stereoselectivity when it is used as a catalyst. We could not observe the near-perfect selectivities of the Tan catalyst 8 in the base-induced reaction of anthrones and maleimides. Nevertheless ee values of up to 83% for the formal Michael products allowed us to isolate 3 compounds in almost enantiopure form after a single recrystallization. The Tan guanidine 8 [19], the bisoxazoline 33 [28], and a stereochemically related bisamidine [27] consistently favor the S,S isomer of Diels-Alder adduct 25.
Although guanidine 10 has the opposite configuration, it also forms S,S-25 preferentially. Accordingly, transition-state structures in the reactions of guanidines 8 and 10 must differ from each other.