Synthesis and post-functionalization of alternate-linked-meta-para-[2n.1n]thiacyclophanes

In recent decades, considerable research attention has been devoted to new synthetic procedures for thiacyclophanes. Thiacyclophanes are widely used as host molecules for the molecular recognition of organic compounds as well as metals. Herein, we report the selective and high-yielding synthesis of novel alternate-linked-meta-para-thiacyclophanes. These novel thiacyclophanes are selectively synthesized in high-yielding procedures. Furthermore, post-functionalization of the phenolic moieties was successfully performed. The 3D structure of the alternate-linked-meta-para-[22.12]thiacyclophane was further elucidated via X-ray crystallographic analysis.


Introduction
The ability of cyclophanes to form three-dimensional cavities is interesting for various potential applications, e.g., as supramolecular hosts. Synthetic procedures towards novel cyclophanes have attracted much interest in the scientific community [1][2][3][4].

Results and Discussion
Macrocyclization Macrocyclization and post-functionalization of cyclophanes is of high interest for the development of various applications in molecular recognition. Based on our previous one-pot proce-dure towards homothiacalixarenes [12], we now report the development of an alternate meta-para-thiacyclophane which could be post-functionalized.
The optimization study was further conducted under diluted conditions and shorter reaction time ( Table 1, entries 2-5). As summarized in Table 1, the reaction is strongly affected by time, base and solvent. At higher concentrations, a decreased yield of 6 is observed. A more significant effect on the selectivity towards 7 was observed by screening different bases (Supporting Information File 1, Table S1, entries 2, 3, 9, and 10). Interestingly, stronger bases let to the almost exclusive formation of the [3 + 3] adduct 7 (Table 1, entry 3) over [2 + 2] adduct 6. A higher proportion of macrocycle 6 was obtained using K 2 CO 3 as the base and toluene as the solvent, the yield rose from 14% (Table 1, entry 2) to 21% (Table 1, entry 4). Working at low concentrations (4 mM) and under O 2 free conditions gave rise to a significant increase in the formation of 6 ( Table 1, entry 5). The [3 + 3] adduct 7 is significantly disfavoured under these conditions. On the basis of these data it could be hypothesized that the [2 + 2] adduct 6 is the more thermodynamically favoured product and the [3 + 3] adduct 7 is the kinetic product. When the reaction was carried out at higher temperatures, an increase in the formation of open chain oligomers was observed (Supporting Information File 1, Table  S1, entries 13 and 14). A longer reaction time at low concentration resulted in a higher yield of the [2 + 2] adduct 6. After   entry 7). Filtration of the precipitate followed by successively washing with water and methanol resulted in an isolated yield of 91%. The 3D structure of macrocycle 6 was confirmed by single crystal X-ray diffraction and shows approximate twofold rotational symmetry (point group C 2 , Figure 1). The dihedral angles between the aromatic rings are given in Table S2 (Supporting Information File 1, ring numbering as shown in Figure 1). The conformation in the solid state is stabilised by intramolecular O-H···S hydrogen bonds (O···S distances 3.2081 (16) and 3.4179 (17) Å) and shows no central void.
The formation of the [3 + 3] product is favoured by stronger bases and shorter reaction times. Therefore, in entry 8 of Table 1, we report our method of choice to selectively synthe-size 7. Purification of [3 + 3] adduct 7 was successful via precipitation (CHCl 3 /MeOH) in good yield (64%).

Proposed mechanism and stability
In Scheme 3 a reaction mechanism for the formation of the macrocycles is proposed. It is believed that after deprotonation of the OH group and subsequent chloride loss of 4 an o-quinoid structure 9 is formed in situ, that quickly reacts with the deprotonated thiol 10 via Michael addition (Scheme 3, route A) [28][29][30]. However, as aromatic thiols at the benzylic position are good leaving groups, conversely β-elimination (route B) can easily occur, leading to reversal of the addition reactions.
Changing the biselectrophile precursor to anisole derivative 13, for which the ortho-quinoid formation is not possible, did not led to the formation of macrocycle 14 or 15 under the optimized conditions, supporting the proposed mechanism (Scheme 4). Mainly starting material was observed in the reaction mixture.
The syntheses of various phenolic thiamacrocycles are reported under basic conditions [31][32][33][34]. To the best of our knowledge, reactions of thiamacrocycles under acidic conditions have not been reported in contrast to homooxacalix[n]arenes, for example [35,36]. A reaction under acidic conditions with precursor 16 was investigated based on similar conditions as reported by Cragg et al. [35]. The reaction in the presence of p-toluenesulfonic acid (0.05 equiv) led mainly to oligomerization, although in the 1 H NMR spectrum traces of macrocycles 6 and 7 were observed (Scheme 5).

Post-functionalization
As the macrocycles are not stable in solution or in basic medium, most of the initial attempts to post-functionalization with ethyl bromoacetate (17) resulted in complex reaction mixtures.
Reactions at room temperature or higher temperature (25-60 °C) mainly resulted in the transformation of the [2 + 2] adduct 6 to the functionalized [3 + 3] adduct 19. Traces of unidentified oligomers and functionalized [2 + 2] macrocycle were also observed in the reaction mixture. It can be argued that, due to steric hindrance, the alkylation of macrocycle 6 is slow. The less sterically hindered cyclic trimer 7 and the linear oligomers are therefore faster alkylated and removed from the equilibrium between macrocycle 6 and 7. Macrocyclization under the optimal conditions (Table 1) in a one-pot procedure also led to a shift towards the functionalized [3 + 3] adduct 19. Purification of these complex reaction mixtures was not successful. Therefore, in order to prevent β-elimination, lower temperatures were applied. Cooling of the reaction mixtures to −20 °C had a positive effect on the stability of the macrocycles. Despite this, no full conversion was obtained, even with a strong base (NaH). Further exploration of the reaction conditions towards the functionalized macrocycles 18 and 19 indicated that the reaction proceeds best at 0 °C, using NaH as a base combined with a large excess of the appropriate electrophile 17 (

Conclusion
In conclusion, a selective procedure was developed towards alternate-linked-meta-para-thiacyclophanes. Starting from readily available materials, the [2 + 2] adduct 6 was synthesized in a high-yielding protocol. A major benefit of the procedure is the simple work-up as the product precipitates from the reaction mixture. Furthermore, it was also possible to selectively synthesize the [3 + 3] adduct 7 in good yield, while avoiding chromatography. The unfunctionalized macrocycles are labile in neutral solution or basic medium. However, postfunctionalization of the macrocycles was successfully realized at low temperatures and with a large excess of the electrophile. Functionalization of the [2 + 2] macrocycle 6 towards an amide derivative 20 and acid derivative 21 was performed with good overall yields (three steps), 80% and 54%. In the near future, the binding properties of these interesting alternate-linked-metapara-thiacyclophanes will be investigated.

Supporting Information
Supporting Information File 1 Experimental part.