Flexible synthetic routes to poison-frog alkaloids of the 5,8-disubstituted indolizidine-class I: synthesis of common lactam chiral building blocks and application to the synthesis of (-)-203A, (-)-205A, and (-)-219F

Background The 5,8-disubstituted indolizidines are the largest class of poison-frog alkaloids found in anuran skin, and are of considerable interest because of their inhibitory effects on the neuronal nicotinic acetylcholine receptors. Many synthetic strategies for the construction of this nucleus have been reported: however, a flexible route has not been reported to date. Results Synthesis of lactam chiral building blocks for the flexible synthesis of the title alkaloids has been achieved using a Michael-type conjugate addition reaction to a chiral cyclic enamine ester as the key step in constructing the trisubstituted piperidine ring system. To demonstrate the usefulness of these chiral building blocks, syntheses of (-)-203A, (-)-205A from 1, and (-)-219F from 2 have been achieved. Conclusion The total synthesis of (-)-203A, (-)-205A, and (-)-219F was achieved, and the absolute stereochemistry of natural 203A was determined to be 5S, 8R, 9S. In addition, the relative stereochemistry of natural 219F was determined.


Introduction
The indolizidine ring system has been widely found in microbial, plant, and animal sources, and many natural products containing this ring system show interesting biological activities. [1] The skin extracts of poison-frogs are a rich source of indolizidines. [2] There are about 20 examples of 3,5-disubstituted indolizidines and about 80 of the 5,8-disubstituted indolizidines. Furthermore, many of such poison-frog alkaloids show significant activities, for example with nicotinic acetylcholine receptors (nAChRs) of the central nervous system. [3] Our syntheses and then biological evaluations of poison-frog alkaloids, [4][5][6][7][8][9][10] revealed that the 5,8-disubstituted indolizidine (-)-235B', exhibited selective and potent blockade of α4β2-nAChRs. [11] Alkaloids of this class with various substituents at the 5-and 8-positions that have been synthesized are shown in Figure 1. All side-chain double bonds in these synthetic compounds have the cis (Z) configuration.
Our flexible synthetic strategy provides a powerful tool for the synthesis of 5,8-disubstituted indolizidines, permitting detailed investigation of structure activity relationships for blockade of nAChRs by this class of alkaloids.
In this contribution, we describe the synthesis of the common lactam chiral building blocks that permit the flexible synthesis of 5,8-disubstituted indolizidines. Their application to the synthesis of (-)-203A, (-)-205A, and (-)-219F illustrates in detail the synthetic procedures employed. [12]

Results and Discussion
To realize the versatile synthesis of the 5,8-disubstituted indolizidine class of poison-frog alkaloids, we designed two lactam chiral building blocks (1,2). The substituent at the 8-position is stereoselectively created by our original Michael-type conjugate addition reaction. [13] Various substituents at the 5-position would be introduced using the protected hydroxymethyl side-chain ( Figure 2). The synthesis began with the known piperidone 3, [14] which was treated with n-BuLi and then CbzCl to provide the Cbz-urethane 4. Treatment of 4 with LiHMDS followed by 2-[N,N-bis(trifluoromethylsulfonyl)amino]-5chloropyridine (Comins' reagent) [15] gave the enoltriflate 5 in good yield. The palladium-catalyzed carbon monoxide insertion reaction [16] in the presence of MeOH afforded the enaminoester 6. The key Michael-type conjugate addition reaction of 6 with lithium dimethylcuprate or divinylcuprate proceeded smoothly to provide the trisubstituted piperidines (7,8) as single stereoisomers in excellent yields.
The stereochemical course of the above addition reaction can be rationalized as follows: The enamine ester 6 would adopt conformation A owing to A (1,3) strain [17] between the benzyloxycarbonyl group on nitrogen and the substituent at the α-position, The methyl or vinyl anion then attacks from the α-orientation controlled by a stereoelectronic effect [18] producing the desired trisubstituted piperidine as a single isomer. This argument can also be explained by the Cieplak's hypothesis [19] as shown in Figure 3. Reduction of the ester moiety in 7 and 8 with Super-Hydride gave the corresponding alcohols (9, 10) in good yield. Swern oxidation of 9 or 10 followed by Horner-Emmons reaction of the resulting aldehydes afforded the α,β-unsaturated esters (11, 12) each in 97% yield. Hydrogenation of the double bond in 11 or 12 over 20% Pd(OH) 2 and then treatment of the resulting deblocked amino alcohols with trimethylaluminum under Weinreb's conditions [20] gave rise to the lactams 1 and 2 in 71% and 68% overall yields, respectively. To demonstrate the utility of the chiral lactam building blocks, we conducted the total synthesis of indolizidines (-)-203A [21] and (-)-205A [22]   In addition, (-)-219F, a 5,8-disubstituted indolizidine with an ethyl group at C-8, [2] was synthesized from 2 (Scheme 4). The lactam 2 was converted to the homologated alcohol 20 via 19 as used with 1 in the synthesis of (-)-205A, which was then transformed into (-)-219F using the Seyferth-Gilbert reaction after Swern oxidation of 20.
Although the direct comparison of the NMR spectra of the synthetic alkaloid with the natural product was not possible due to the scarcity of natural product, the GC-MS and GC-FTIR spectra of the synthetic material were identical with those of natural product detected in the Madagascan mantellid frog, Mantella betsileo. Thus, the relative stereochemistry of natural 219F was established.
In conclusion, we succeeded in the construction of chiral lactam building blocks (1, 2) for the synthesis of three representative poison-frog alkaloids of the 5,8-disubstituted indolizidine class; these were alkaloids (-)-203A, (-)-205A, and (-)-219F (experimental details can be found in Additional File 1). This flexible synthetic route starting from 1 or 2 will be amenable to any side-chain at the 5position of these alkaloids. Such indolizidines are expected to show inhibitory effects on the nAChRs, and the biological results will be reported in due course.