Amine-linked diglycosides: Synthesis facilitated by the enhanced reactivity of allylic electrophiles, and glycosidase inhibition assays

Diglycose derivatives, consisting of two monosaccharides linked at non-anomeric positions by a bridging nitrogen atom, have been synthesised. Conversion of one of the precursor monosaccharide coupling components into an unsaturated derivative enhances its electrophilicity at the allylic position, facilitating coupling reactions. Mitsunobu coupling between nosylamides and 2,3-unsaturated-4-alcohols gave the 4-amino-pseudodisaccharides with inversion of configuration as single regio- and diastereoisomers. A palladium-catalysed coupling between an amine and a 2,3-unsaturated 4-trichloroacetimidate gave a 2-amino-pseudodisaccharide as the major product, along with other minor products. Derivatisation of the C=C double bond in pseudodisaccharides allowed the formation of Man(N4–6)Glc and Man(N4–6)Man diglycosides. The amine-linked diglycosides were found to show weak glycosidase inhibitory activity.


Introduction
We have been interested in synthesising molecules consisting of two monosaccharides linked by formal condensation without using the anomeric position [1]. Such molecules, termed diglycoses (or neodisaccharides), are linked by ether, amine, thioether, selenoether, etc., bridges, and so are presumably more stable to hydrolysis by acid or glycosidases than (glycosidic) disaccharides. Diglycoses have many features in common with disaccharides, with a similar general appearance, size, and functional group pattern, i.e., polyhydroxylation with potentially hydrophobic areas above and below the ring plane. Synthetic structures of this type have been known since the 1960s [2,3], and have attracted sporadic attention since then, often in the context of proposed carbohydrate or disaccharide mimicry. Last year, we reported that neutral ether-and thioether-linked diglycose derivatives can interact with lectins with affinities similar to those of strongly binding disaccharide ligands [4]. In the related carbasugar series, Ogawa et al. have shown that pseudodisaccharides with a bridging nitrogen atom bind more strongly to glycosidases than do the corresponding ether or thioether derivatives [5]. It follows that amine-linked diglycose derivatives may act as glycosidase inhibitors. We set about the synthesis of some compounds of this type to test this hypothesis.
In our initial investigations into this area [6] we found that the installation of amine linkages between primary-primary carbons of monosaccharides was relatively straightforward; this was achieved by Mitsunobu coupling between carbohydrate C-6 alcohols and carbohydrate C-6 sulfonamides. Primary-secondary linkages were more difficult to synthesise; attempted Mitsunobu coupling failed, but we synthesised such amines by epoxide-opening. We did not achieve the formation of sec-sec linked secondary amines. The synthesis of a sec-sec aminelinked diglycoside structure by epoxide-opening was reported by Coxon [7], its formation being achieved in low yield (25%) under rather harsh conditions (autoclave, 140 °C), while a similar epoxide-opening reaction starting from a rather more complex substrate has been used to synthesise a pseudohexasaccharide in very low yield (12%) [8]. Kroutil et al. reported the synthesis of a number of aminated amine-linked sec-sec structures by aziridine-opening reactions (ionic liquid, 120 °C) [9]. Primary-sec amine-linked structures have been synthesised by Thiem et al., through a reductive amination strategy [10,11].
In contrast, related structures containing an amine linkage between a carbohydrate ring carbon (i.e., a secondary position) and C-1 of either a (C-5a methylene) carbasugar or a C5=C5a unsaturated carbasugar, are relatively common [12]. There are two possible contributing factors to this: First, these classes of carbasugar or valienamine structures have been more widely studied, possibly due to their presence in natural products and well known biological activities as glycosidase inhibitors [13]; second, electrophiles that are either lacking a bulky and electron-withdrawing substituent at one beta position [14] (making them less carbohydrate-like), or that are allylic [15], would tend to be intrinsically more reactive than similar carbohydrate electrophiles, thus facilitating the coupling reaction.
Bearing in mind the limitations discovered in our earlier work, we planned to enhance the reactivity of the electrophilic component in our coupling reactions by converting a carbohydrate into an unsaturated derivative with an allylic alcohol as leaving group [16]. After the coupling reaction, dihydroxylation of the C=C double bond would restore the carbohydrate structure (Scheme 1) [17]. As well as Mitsunobu chemistry [18], the allylic nature of the electrophile opens up the way for transitionmetal-catalysed allylic amination reactions [19][20][21] as a possible coupling method. We report our investigations into this area in this paper. There is extensive coverage in the literature of the derivatisation of primary carbohydrate alcohols by Mitsunobu type reactions [22]. Reports of Mitsunobu reactions of secondary alcohols in fully functionalized carbohydrates are much scarcer. Rather, reports exist of failed attempts at Mitsunobu reactions of secondary carbohydrate alcohols [23] or the selective functionalisation of primary carbohydrate alcohols in the presence of secondary alcohols [24]. Some examples of successful reactions do exist, though, for oxygen, nitrogen and sulfur nucleophiles [25][26][27][28]. Unsaturated carbohydrates, similar to those described here, have also been reported to undergo Mitsunobu reaction with simple, non-carbohydrate nucleophiles [29,30]. The palladium-catalysed allylic amination reaction on unsaturated pyranose rings was pioneered thirty years ago by Hanna and Baer [19,20], and has more recently been reinvestigated with rather simple nitrogen nucleophiles [30,31]. Carbohydrate amines have been used as nucleophiles in allylic amination by Shing to form valienamine pseudodisaccharides [21].

Results and Discussion
The synthesis of the 2,3-unsaturated erythro carbohydrate derivative 1 was carried out from triacetyl glucal essentially according to the literature procedure [30]. The threo alcohol 2 has been previously synthesised by Mitsunobu inversion of the erythro alcohol 1 [30]. We synthesised 2 from triacetyl galactal 3 by an analogous route to that used for the erythro alcohol 1 (Scheme 2). We found that the choice of Lewis acid used in the Ferrier reaction of 3 with ethanol was critical for a satisfactory yield of the unsaturated glycoside 4 to be achieved; phosphomolybdic acid [32] gave the product (α:β, 8:1) in 63% yield. Deacetylation of 4 and regioselective silylation of the primary alcohol gave the threo allylic alcohol 2. The sulfonamide nucleophiles 6, 7 and 9 were prepared from the corresponding amines 5 [33] and 8 [34], as described previously [6], with only one equivalent of the sulfonylating agent so as to avoid bissulfonamide formation.
Mixing equimolar equivalents of the erythro allylic alcohol 1 and the glucose-6-nosylamide 6 with DIAD and triphenylphosphine resulted in a smooth coupling reaction to give the pseudodisaccharide 10 in high yield (Scheme 3). The alcohol 1 also underwent similar coupling with the glucose-6-triflamide 7 and the mannose-6-nosylamide 9 under the same Mitsunobu conditions to give the respective diglycoside precursors 11 and 12 in excellent yields. These were the same reaction conditions that had failed to give coupling reactions between a primary alcohol and a sulfonamide at a secondary carbohydrate position, or a secondary alcohol and a sulfonamide at a primary carbohydrate position, in our earlier study [6]. Coupling of this threo alcohol 2 with sulfonamide nucleophiles (6, 7 and 9) again gave the protected secondary amines (13)(14)(15) in excellent yield. No difference in the efficiency of the coupling reactions of the nosylamide 6 and the triflamide 7 was seen with either of the alcohols 1 or 2.
Assignment of the NMR spectra of the coupling products 10-15 by the usual 2D methods (COSY, HSQC) gave an unambiguous indication that, in all cases, the nitrogen nucleophile had attacked C-4, as judged by an upfield shifted 13 C signal, and that the unsaturated bond was between C-2 and C-3. Hence these reactions proceeded with essentially complete regioselectivity, with no S N 2' products arising from attack at C-2 being seen. The coupling reactions were stereospecific: The nucleophile 6 coupled with the threo alcohol 2 to give the coupling product as a single diastereomer 13; but 6 also coupled with the diastereomeric erythro alcohol 1 to give a different product 10, again as a single diastereomer. This is consistent with an S N 2 pathway with inversion of configuration, which is as expected for Mitsunobu reactions. The same was true of the other sulfonamide nucleophiles, 7 and 9. Moreover, the erythro configuration of coupling product 15 was proved by the large value of the J 4,5 coupling constant (9.8 Hz), which can only be explained by a trans relationship of the 4-H and 5-H protons and a O H 5 half-chair conformation. The J 4,5 coupling constants for the products assigned as threo were not always well resolved, but in 12, a smaller coupling constant (ca. 4 Hz) was seen.
Having achieved the coupling, we turned to the refunctionalisation of the C=C bond (Scheme 4). Osmium-catalysed dihydroxylation of the double bond in erythro configured pseudodisaccharide 13 proceeded to give a single diastereomer of product 16. The configuration of the dihydroxylated product 16 was most conveniently assigned after conversion to its diacetate 18, which resulted in a downfield shift of the 2-H and 3-H signals into a clear region of the 1 H NMR spectrum, allowing readout of the coupling constants without any signal overlap. The values of J 1,2 (1.8 Hz), J 2,3 (3.3 Hz), and J 3,4 (11.0 Hz) are consistent with the α-manno configuration, and inconsistent with the alternative α-allo configuration. Hence dihydroxylation proceeded from the opposite side to the two neighbouring substituents (at C-1 and C-4), as expected in a sterically controlled reaction, and consistent with previous results [35]. The protecting groups were then removed. First, the TBDMS ether was removed with acid to give the triol 19. Alternatively, 19 was formed directly in the dihydroxylation reaction by allowing a longer reaction time (20 h) after quenching with NaHSO 3 , leading to a similar overall yield (80% from 13). Attempted TBAF deprotection of the silyl ether in the dihydroxylation product 16 gave only a low yield of the triol 19. Subsequently, the benzyl ethers and nosylamide in 19 were cleaved under Birch reduction conditions to furnish the free Man(N4-6)Glc diglycoside 21. The other erythro configured pseudodisaccharide 15 behaved in a similar way, and we obtained the Man(N4-6)Man diglycoside 22 following the same reaction sequence (Scheme 4).
However, attempted functionalisation of the C=C double bond in the threo configured pseudodisaccharides 10 and 12 under the same conditions, or using stoichiometric K 2 OsO 4 with NMO, failed to give any reaction. A combination of RuCl 3 and NaIO 4 produced, after 4 days, a mixture of starting material and desilylated material but no oxidised product. Treatment with mCPBA failed to give an epoxide, and after a week only some decomposition of the starting material was seen. Presumably, the trans relationship between the flanking NNsR and OEt groups effectively crowds both faces of the olefin, blocking its reactivity.
We briefly examined palladium-catalysed allylic amination as a possible coupling procedure. The allylic alcohol in the threo derivative 2 was converted into its trichloroacetimidate 23 by treatment with trichloroacetonitrile and base. The trichloroacetimidate is a leaving group that has the advantage of being easily and stereospecifically synthesised from the alcohol under basic conditions. We recently used the trichloroacetimidate as leaving group in palladium catalysed synthesis of carbasugar pseudodisaccharides [36]. Treatment of the imidate 23 with an amine nucleophile 5 [33] and a palladium catalyst [21] resulted in the formation of four products, 24 (44%), 25 (15%), 26 (5%) and 27 (6%), which were assigned the structures given in Scheme 5.
A further characteristic feature that can be used to distinguish 2-amino-3,4-unsaturated-α-threo configured compounds from their α-erythro C-2 epimers is the J 1,2 coupling constant. Ferrier pointed out that α-threo diastereomers have very small J 1,2 coupling constants, i.e., 1-H usually appears as a singlet, whereas the α-erythro diastereomers have J 1,2 between 3.5-4.5 Hz [44]. In 24, 1-H appeared as a singlet, consistent with our assignment of α-threo stereochemistry. The C-4 stereochemistry of the two 4-amino derivatives 25 and 26 was assigned on the basis of the Hence, the major products 24 and 25 from the palladium-catalysed reaction are those with overall retention of configuration as expected from the double displacement mechanism. Under these unoptimised reaction conditions though, the palladiumcatalysed reaction was much less regioselective than the Mitsunobu couplings. Moreover, the major regioisomer in the palladium-catalysed reaction was the 2-amino sugar, whereas in the Mitsunobu reactions, the 4-amino sugars were formed exclusively. A small amount of stereochemical scrambling of an intermediate π-allyl palladium complex could explain the formation of 26.
Finally, we report the results of assays of the unprotected diglycosides Man(N4-6)Glc 21 and Man(N4-6)Man 22, along with those we had synthesised earlier [6], viz., Alt(N2-6)Glc 28, Alt(N2-6)Man 29, Alt(N3-6)Glc 30, Alt(N3-6)Man 31, Glc(N6-6)Glc 32, and Glc(N6-6)Man 33 (Figure 1), for inhibitory activity against glycosidases. Screening the compounds against twelve commercially available glycosidases (see Supporting Information File 1) [45,46], we found that none of the compounds had a high inhibitory activity against any of the glycosidases when tested at 1 mM inhibitor concentration. The results for those enzymes for which some inhibitory activity was seen arising from one or more of the diglycosides, are given in Table 1. These diglycosides showed weaker glycosidase inhibitory activity than the related N-substituted (monosaccharidic) altrosides recently reported by Jenkins et al. [47]. These results are in accordance with those reported for other disaccharide mimetics, such as aza-C-disaccharides, which showed no significant inhibition towards commercially available glycosidases [48].
We also investigated inhibition of α-glucosidase II using the oligosaccharide Glc 2 Man 7 GlcNAc 2 as substrate and using the free oligosaccharide (FOS) assay in cultured cells [49]. The Alt(N2-6)Man derivative 29 showed some inhibitory activity, with IC 50 ca. 500 μM. None of the other compounds tested inhibited the enzyme at 100 μM concentration.
Such amine-linked diglycosides have not been shown to have intrinsic biological activity before. However, the inhibitory activity, where it exists, is weak for these compounds, and the nature of the binding and the basis for inhibition is not clear. α-Glucosidase II cleaves two linkages in nature in the biosynthesis of N-linked glycoproteins: Glc(α1→3)Glc and Glc(α1→3)Man. The Alt(N2-6)Man structure does not bear an immediately obvious resemblance to either of these substructures. Moreover, it has been proposed that a mannose-binding lectin domain of the α-glucosidase II β-subunit is also important for its activity [50], and the possibility that the inhibitor is actually binding not to the enzyme active site, but to this lectin domain instead, is not ruled out at present.

Conclusion
Unsaturated carbohydrate derived electrophiles react readily with carbohydrate nitrogen nucleophiles to form amine-linked pseudodisaccharides. Triflamides and nosylamides were similarly effective as nucleophiles in Mitsunobu coupling reactions with allylic alcohols. The Mitsunobu coupling reactions proceeded with inversion of configuration, and S N 2' type reac-tions with C=C double bond migration were not observed. A palladium catalysed coupling reaction between an amine and an allylic trichloroacetimidate also gave a high yield of a mixture of amine-linked pseudodisaccharides, but under the conditions we used, much lower regioselectivity and diastereoselectivity were seen than in the Mitsunobu reactions. Completion of the diglycosides by refunctionalisation of the C=C double bond was successful for some diastereomers, but not for others. Glycosidase inhibitory activity of the compounds synthesised here, and also of structurally related compounds synthesised previously by us, was weak.