New tridecapeptides of the theonellapeptolide family from the Indonesian sponge Theonella swinhoei

Summary Chemical analysis of the organic extract of Theonella swinhoei yielded two new tridecadepsipeptides of the theonellapeptolide family, namely sulfinyltheonellapeptolide, characterized by a methylsulfinylacetyl group at the N-terminus, and theonellapeptolide If, the first member of this class of compounds to show four valine residues. The structures of the compounds, isolated along with the known theonellapeptolide Id, were determined by extensive 2D NMR and MS/MS analyses followed by application of Marfey’s method. The isolated peptides exhibited moderate antiproliferative activity against HepG2 cells, a hepatic carcinoma cell line.


Introduction
Three decades of extensive chemical investigation [1] have clearly evidenced that marine sponges of the genus Theonella (Lithistida, Theonellidae) are treasure troves of secondary metabolites. The chemical diversity of the isolated compounds ranges from unusual steroids (exemplified by the 4-methylene sterol theonellasterol [2,3] and truncated side-chain sulfated steroids [4]), to complex macrocyclic polyketides (as the wellknown swinholide A, now a reference compound in the class of actin interacting cell growth inhibitors [5]), polyene derivatives (as aurantosides [6]), and polypeptides/depsipeptides. The biosynthesis of several secondary metabolites of Theonella has been ascribed to symbiotic microorganisms, as in the case of the polyketide onnamide [7] and the polypeptide polytheonamide [8]. It is, however, not unreasonable to presume that a symbiotic role in the production of secondary metabolites could be crucial in many other cases. Probably the most distinctive class of secondary metabolites of Theonella is given by complex polypeptides whose uncommon amino acids have been postulated to have either nonribosomal (NR) or post-translationally modified ribosomal (PMR) origin [8]. Several classes of Theonella polypeptides have been isolated to date, and they often show peculiar features such as largely rearranged amino acidic units, either D-or L-configurations at the α-carbons, and the formation of macrocycles through amide or ester bonds. To categorize the plethora of Theonella polypeptides we could identify at least eight structural types: theonellamides (glycosylated imidazole-containing macrocycles) [9], keramamides (including oxazole or thiazole rings) [10], papuamides (HIV inhibitory macrocyclic depsipeptides) [11], polytheonamides (cytotoxic linear polypeptides) [12], cyclotheonamides (thrombin and serine protease inhibitors) [13], perthamides (anti-inflammatory cyclopeptides) [14,15], solomonamides [16] and theonellapeptolides (cyclic tridecapeptides including several N-methylated and D-amino acids) [17].
In the context of our long-standing interest for bioactive secondary metabolites from marine invertebrates, we have recently investigated a specimen of T. swinhoei collected off the coasts of Manado (North Sulawesi, Indonesia), which proved to be rich in aurantosides [6] and 4-methylene steroids [18], while polyketide macrolides and peptide-based derivatives were extremely rare if not absent. Remarkably, the chemical analysis of a different specimen of T. swinhoei, collected in the same area as the previous one, exhibited an extremely different secondary metabolite composition. In particular, this second specimen has been found to be rich in polypeptides and, from the CHCl 3 phase of the organic extract, we have identified the major member of this class as theonellapeptolide Id (1) (Figure 1). In this paper we report the results of the chemical analysis of the polypeptide-containing fraction, which resulted in the isolation of two new theonellapeptolides, namely sulfinyltheonellapeptolide (2) and theonellapeptolide If (3) (Figure 2). We describe the structural elucidation of these new metabolites and the results of the preliminary pharmacological evaluation of the isolated compounds on HepG2 cells (hepatocarcinoma cell line).

Results and Discussion
Theonella swinhoei was collected in the Bunaken Marine Park of Manado (North Sualwesi, Indonesia) in January 2010 and frozen immediately after collection. The frozen material was repeatedly extracted with methanol, and the crude extract was subjected to a modified Kupchan's partitioning procedure [19] to obtain n-hexane, CHCl 3 and n-BuOH extracts. The CHCl 3  Figure 2). The known compound was identified on the basis of the comparison of its spectroscopic data with those published in the literature [17].
Theonellapeptolides are tridecapeptide lactones characterized by the presence of aliphatic and non-polar amino acids including high ratio of D-amino acids, N-methyl amino acids, and β-amino acids. In particular, the structure of theonellapeptolide Id (1) includes three β-Ala, three D-Leu (one N-methylated), two L-Val (one N-methylated), one L-MeAla, one L-Thr, one L-MeIle, and two D-alloIle (one N-methylated) units.
Sulfinyltheonellapeptolide (  placed it into the theonellapeptolide class. Given this characterization of 2 the presence of the sulfur atom in the molecular formula appeared especially remarkable. In particular, the 1 13 C NMR chemical shift values of both the methyl and the methylene groups strongly suggested their linkage to a sulfoxide group, and the presence of a methylsulfinylacetyl (MeS(O)Ac) subunit was also in full agreement with the molecular formula. The amino acid sequence of 2 was then disclosed by careful analysis of the pattern of HMBC correlations, whose key cross-peaks are shown in Figure 3. The cross-peak of the Thr low-field shifted β-CH (δ H 5.19) with the Me-Ile carbonyl group revealed the presence of an ester linkage.
HMBC analysis also revealed that the amide carbonyl at δ C 166.5 assigned to the methylsulfinylacetyl subunit was correlated to the Hα of Val-1 residue, thus establishing the acetylation of the N-terminus in 2. Methanolysis of 2, followed by ESI (positive ion mode) MS/MS analysis of the obtained acyclic methyl ester derivative provided key fragment peaks, shown in Figure 3, giving definitive confirmation of the sulfinyltheonellapeptolide gross structure. In addition to the pseudomolecular ion at m/z 1454 [M + H] + , corresponding to the introduction of 32 mass units (MeOH) in the molecule, the ESIMS/MS spectrum provided several fragment ion peaks corresponding to N-terminus fragments due to the cleavage of the amide bond, and referred to as the b series in Roepstorff and Fohlman nomenclature [20]. In particular, the presence of the b2 fragmentation peak at m/z 331 supported, once again, the presence of the MeS(O)Ac unit. Similarly, it appeared reasonable to assume that, as in the co-occurring theonellapeptolide Id (and in all the theonellapeptolide found to date), the L-Val could be the N-terminal amino acid and thus, the D-Val should be the amino acid at positions 28-32. A D-Val residue at these positions has been found previ-  3). These data were easily rationalized with the replacement of the terminal methylsulfinylacetyl group with a methoxyacetyl group, a typical N-terminus acylating unit, already found in the theonellapeptolide family.
Careful inspection of COSY and TOCSY spectra revealed also that, in agreement with the molecular formula, one isoleucine residue of 2 is replaced by a valine residue in 3. A subsequent combined analysis of homonuclear and heteronuclear 2D NMR spectra disclosed the presence of the following residues: three β-Ala, three Leu (one N-methylated), one N-methyl-Ile, four Val (two N-methylated), one Thr, and one N-methylAla. As detailed for 2, the amino acid sequence of 3 was deduced by careful analysis of HMBC correlations and supported by the MS/MS spectrum of the methanolysis product mixture (see Supporting Information File 1). Thus, theonellapeptolide If (3) has been identified as a new member of the theonellapeptolide family differing from theonellapeptolide Id (1)  Theonellapeptolides are structurally similar to the widely used immunosuppressant drug cyclosporin A, sharing at least three important features: i) the exclusive presence of hydrophobic aliphatic amino acids; ii) the abundance of N-methylated residues and iii) the presence of a macrocycle made up by more than ten amino acids. Only a few theonellapeptolides have been tested for immunosuppressive activity and they revealed a moderate potency [25]. In general, the potential bioactivity of this class of unique cyclopeptides has not been investigated in great detail, and an inhibition of the development of fertilized eggs of sea urchin [26], an activity on the transport of Na + and K + ions [23], and a moderate cytotoxicity [23] have been reported.
The three theonellapeptolides isolated during the present investigation (1-3) have been evaluated for their antiproliferative activity against HepG2 cells, a hepatic carcinoma cell line. As reported in Figure 4, all tested compounds showed antiproliferative activity at low micromolar doses, with a similar pattern of potency. At the dose 10 μM, the proliferation rate of HepG2 cells was significantly reduced by theonallapeptolide Id (1), sulfinyltheonellapeptolide (2) and theonellapeptolide If (3) to about 50%, 30% and 50%, respectively ( Figure 4, panels A, B and C; * p<0.05 compared to untreated cells; n = 4). Only theonellapeptolide Id was able to significantly reduce the proliferation at 1 μM. At this dose, the proliferation reduction was equal to 20% (Figure 4, panel A; * p < 0.05 compared to untreated cells; n = 4). Computation of 50% inhibitory drug concentration (IC 50 ) revealed that compounds 1, 2 and 3 were characterized by very similar IC 50 values of about 1.5 μM for theonellapeptolide Id and 3 μM for sulfinyltheonellapeptolide and theonellapeptolide If.

Conclusion
In conclusion, the chemical analysis of the organic extract of an Indonesian Theonella swinhoei allowed the isolation of two new members of the theonellapeptolide family, namely sulfinyltheonellapeptolide, characterized by a methylsulfinylacetyl group at the N-terminus, and theonellapeptolide If, the first member of this class to show four valine residues. The structures of the compounds have been determined by extensive 2D NMR and MS/MS analyses followed by the application of Marfey's method. The position of D-and L-residues was also assigned on the basis of a comparison with known compounds.
A moderate antiproliferative activity on a hepatic carcinoma cell line has been disclosed for the isolated compounds. A more detailed evaluation of the pharmacological profile of this class of marine peptides is underway in our groups.  Kupchan's partitioning procedure as described in [26] Acid hydrolysis and Marfey's analysis: Peptide samples (600 μg) were subjected to acid hydrolysis and subsequent Marfey's analysis in a similar manner as described in [27]. Evaluation of antiproliferative activity: HepG2 cells were plated in a 24-wells plate at 3 × 10 4 cells/well, in Minimum Essential Medium with Earl's salts containing 10% fetal bovine serum (FBS), 1% L-glutamine and 1% penicillin/streptomycin. On day 2, cells were treated with increasing doses of theonellapeptolide Id (0.1, 1 and 10 μM), sulfinyltheonellapeptolide (0.1, 1 and 10 μM) and theonellapeptolide If (0.1, 1 and 10 μM) for 48 hours. On day 4, a MTT assay was assessed: 100 μL of MTT solution (5mg/mL) were added to each well and the cells were incubated at 37 °C for 4 hours. After the incubation, the culture medium was removed and 1 mL of DMSO was added to each well. The absorbance was read by using a spectrophotometer at 590 nM. The proliferation rate was reported as the delta of absorbance compared to untreated cells, and values are expressed as mean ± standard error. Experiments were performed in quadruplicate. For each compound the IC 50 value was evaluated as follows: The inhibition of proliferation was expressed as percentage compared to the inhibition of proliferation observed at the highest dose of any experimental setting (which was arbitrarily assigned a value of 100%).

Supporting Information
Supporting Information File 1 COSY, key HMBC correlations and MS/MS fragmentations of 3, and 1 H and COSY spectra for sulfinyltheonellapeptolide (2) and theonellapeptolide If (3).