A versatile and efficient approach for the synthesis of chiral 1,3-nitroamines and 1,3-diamines via conjugate addition to new (S,E)-γ-aminated nitroalkenes derived from L-α-amino acids

Summary New chiral (S,E)-γ-N,N-dibenzylated nitroalkenes 2a–c were synthesized from natural L-(α)-amino acids in five steps with overall yields of 68–88%. The conjugate addition of hydride, methoxide, nitronate and azide nucleophiles to 2a–c led to the corresponding chiral 1,3-nitroamines in 74–90% yield. The conjugate addition of cyanide anion to 2a,b was followed by HNO2 elimination affording chiral aminated acrylonitriles (73–98%). On the other hand, the azide anion reacted with 2a, in acetonitrile, via a [3 + 2]-cycloaddition in which HNO2 was lost, providing the corresponding 1,2,3-triazole derivative. Direct reduction of 1,3-nitroamine derivatives 9a,b produced the corresponding 1,3-diamines in good yields.


Results and discussion
The preparation of 2a-c (Scheme 1) was initiated with the synthesis of Reetz's α-aminoaldehydes [69,70].In this manner, the α-amino acids 1a-c were perbenzylated using a slight modification in the experimental procedures described by Beaulieu [71] and Warren [72] leading to tribenzylated esters 3a-c in 90-99% isolated yield.Next, reduction of 3a-c with LiAlH 4 provided the desired alcohols 4a-c (87-95% yield), which were oxidized under Swern conditions to aldehydes 5a-c in 96-99% yield.These were submitted to a Henry reaction, according to the modified conditions developed by Hanessian [73].Thus, 5a-c were reacted with nitromethane, at room temperature (instead of 0 °C) in the presence of 0.2 equivalents of TBAF•3H 2 O (instead of 1 equivalent) as base, furnishing an anti:syn-nitroalcohol mixture 6a-c in 91-95% yield and 68-86% d.e.The activation of the hydroxy groups of 6a-c by transformation into the corresponding mesylates was followed by smooth elimination at −78 °C, leading to the desired nitroalkenes 2a-c in 95-99% yield, as single E-diastereoisomers.It is worth mentioning that the nitroalkenes 2a-c are obtained in high chemical and stereochemical purity and can be stored in a freezer or even at room temperature for several months without decomposition occurring.
In order to verify the enantiomeric purity of the new nitroalkenes 2a-c and to confirm their stereochemical stability, a chiral HPLC analysis was conducted.Racemic (+/−)-2b was synthesized from (D/L)-phenylalanine by the same route employed for (−)-2b and utilized as standard (Figure 1).The racemic nitroalkene (+/−)-2b showed a very good separation factor in the chromatograph chiral column used.Figure 1 shows that (−)-2b prepared from L-phenylalanine was enantiomerically pure (enantiomeric excess > 99%).HPLC analysis showed that the stereochemical integrity of 2 was preserved throughout the synthetic process.Next, the reactivity of the new chiral nitroalkenes was investigated with respect to the conjugate addition of hydride, nitronate,  methoxide, cyanide, and azide anions in addition to benzylamine aiming to obtain directly 1,3-nitroamine derivatives.All the experiments were carried out at room temperature and the results are summarized in Table 1.
The selective conjugate addition of hydride anion to 2b produced the chiral 1,3-nitroamine 7b in 85% yield [74] (Table 1, entry 1).Similarly, the addition of nitromethane to 2a,b led to the corresponding 2-nitromethyl-1,3-nitroamine derivatives 8a,b in good yields (Table 1, entries 2 and 3).Other nitronate anions also reacted adequately.On the other hand, a literature search showed that the addition of an oxygenated nucleophile to nitroalkenes has been scarcely studied due to the low reactivity of neutral nucleophiles associated with the reaction reversibility [46,75,76].Addition of methoxide anion to 2a-c led to the corresponding chiral 2-methoxy-1,3-nitroamine derivatives 9a-c in 70-90% yield (Table 1, entries 4-8).A high d.r. was obtained when shorter reaction times were employed (Table 1, entries 4 and 5).On the other hand, a severe decrease in the diastereoselectivity occurred with longer durations, presumably due to an equilibration by a retro-conjugate addition [77] (Table 1, entries 6-8).Next, the conjugate cyanide addition to 2a,b promoted by TMSCN 1 equiv/TBAF•3H 2 O 1 equiv led to 12a,b in 98 and 73% respectively, instead of 2-cyano-1,3-nitroamines derivatives (Table 1, entries 9 and 10).Products 12a,b were likely formed from the conjugate addition of cyanide to 2a,b followed by HNO 2 elimination.It is worthy of note that the Michael addition of "HCN" equivalent to nitroalkenes is rare in the literature [78][79][80][81][82][83].Paulsen [78] and Sakakibara [79] were the first authors to report the cyanide addition to α,β-unsaturated nitroalkenes.In agreement with our results, Sakakibara [79] and Spanevello [80] observed a total or partial HNO 2 elimination from vicinal nitronitrile adducts.This behavior was not observed in other findings reported [78,[81][82][83] and the corresponding vicinal nitronitriles were the sole products.It is important to note that 12a,b can in theory also be obtained by an aza-Baylis-Hillman reaction [15][16][17][18]68].Thus, this cyanide Michael addition to 2a,b constitutes a synthetic alternative to the cases where N-protected aza-Baylis-Hillman adducts need to be obtained.Interestingly, the addition of "HN 3 " promoted by TMSN 3 1 equiv/TBAF•3H 2 O 1 equiv furnished either the 2-azido-1,3-nitroamine-derivatives 10b,c (Table 1, entries 11 and 12) or the triazole derivative 11a (Table 1, entry 13) when THF or CH 3 CN were used as solvents, respectively.The formation of chiral 1,2,3-triazole derivative 11a was proposed to be by way of a [3 + 2]-cycloaddition with the azide group acting as 1,3-dipolarophile, followed by HNO 2 elimination leading to aromatization.There are a few reports in the literature where the azide anion is added to α,β-unsaturated nitroalkenes [8,[19][20][21][22] forming 1,4-adducts [8] or 1,2,3-triazole derivatives [31][32][33].Apparently, the reaction course depends on the temperature and concentration of the azide reagent used.In our case, we observed a dependence on the nature of the solvent.However, an influence of the structure of the nitroalkenes has not been independently investigated.Further experiments must be performed for a better understanding of the reaction course.On the other hand, the neat benzylamine addition, or in the presence of DBU (0.5 equiv) as promoter [84], to 2a,b was unsuccessful (Table 1, entries 14 and 15).This nonreactivity can be explained on the basis of the high tendency toward reversibility presented in conjugate additions of amines to reactive nitroalkenes [15,85,86].As a way of testing the stereochemical stability of the synthesized nitroalkenes in reaction media, 2b was recovered before the reaction was completed (Table 1, entry 3) and the magnitude of the optical rotation of 2b was measured.No loss in enantiomeric purity of 2b was observed in the reaction medium essayed.

Conclusion
An efficient, reproducible and versatile approach for the synthesis of chiral nonracemic 2-substituted-1,3-nitroamines was developed.The high chemical and stereochemical stability of the new chiral nitroalkenes 2a-c and the variety of 1,3nitroamines synthesized provide robustness and generality to the methodology.Since the nitroamines 9a,b were efficiently reduced to the corresponding chiral 2-substituted-1,3-diamines 14a,b, the present route constitutes a versatile access to these important classes of substances in high yields.

Table 1 :
Reactivity of 2a-c with different nucleophiles.
a Diastereoisomeric ratio.b Solution 1 M in MeOH.c No reaction observed.