Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization

• Lisa Landgraf,
• Ines Müller,
• Peter Ernst,
• Miriam Schäfer,
• Christina Rosman,
• Isabel Schick,
• Oskar Köhler,
• Hartmut Oehring,
• Vladimir V. Breus,
• Thomas Basché,
• Carsten Sönnichsen,
• Wolfgang Tremel and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2015, 6, 300–312, doi:10.3762/bjnano.6.28

• monolayers around the CdSe core of QDs showed that the lowest impact on cell metabolism (cellular dehydrogenase activities) was present when QDs were coated with two monolayers (Figure S1, Supporting Information File 1). Impedance measurements (electric cell–substrate impedance sensing, ECIS) revealed a

• carried out 24 h after seeding. All these experimental procedures occured during the electric cell–substrate impedance sensing (ECIS) recording and the 8-well electrode array was placed at 37 °C in the humidified 5% CO2 environment (incubator CO2Cell, Germany). Adherent cells spread on the surface of

• planar gold-film electrodes and increase the impedance of these electrodes [72]. A custom-built ECIS system was employed, consisting of a lock-in amplifier (SR830, SRS, Inc., CA) with an internal oscillator, a multiplexer with analogue switches for automatic, consecutive addressing of individual wells on

The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots

• Vladimir V. Breus,
• Anna Pietuch,
• Marco Tarantola,
• Thomas Basché and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 281–292, doi:10.3762/bjnano.6.26

• -particle tracking), was shown to compromise the integrity of the cytoskeletal and plasma membrane dynamics, as evidenced by electric cell–substrate impedance sensing. Keywords: biocompatibility; CdSe/ZnS; cytotoxicity; ECIS; quantum dots; single-particle tracking; Introduction Quantum dots (QDs) are

• , respectively. Electric cell–substrate impedance sensing (ECIS), which was first described by Giaever and Keese [22], offers a versatile and noninvasive means to monitor cellular adhesion and motility on a subsecond timescale [23][24]. After adhesion and spreading on a gold electrode surface, cells behave as

• ][27]. Furthermore, we combined ECIS with single-particle tracking, which was previously used to follow the intracellular pathway of Tat peptide-conjugated QDs in living cells [28], transport of QD-labelled monoclonal anti-HER2 antibody in mice [29], specific recognition of avidin-CD14 receptor by

Mechanical properties of MDCK II cells exposed to gold nanorods

• Anna Pietuch,
• Bastian Rouven Brückner,
• David Schneider,
• Marco Tarantola,
• Christina Rosman,
• Carsten Sönnichsen and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 223–231, doi:10.3762/bjnano.6.21

• that have been focussed on. Cytoxicity of nanomaterial has been assessed by common cytotoxicity assays targeting enzymatic activity such as LDH, MTT and ECIS. So far, however, less attention has been paid to the mechanical parameters of cells exposed to gold particles, which is an important reporter on

• enzymes. More advanced techniques, like electric cell-substrate impedance sensing (ECIS) or quartz crystal microbalance measurements monitor the vertical cell motility, i.e., dynamic changes of the cell-substrate distance, as a reporter for cell viability [1][10][11][12][13][14][15]. Mechanical properties

• ) and actin-filaments (Figure 1 A–C, IV) reveals that with increasing concentration and incubation time (data not shown) of CTAB-nanorods disassembly of the filaments occurs concomitant with an increase in viability loss. Cytotoxicity studies using ECIS and MTS tests show that at these concentrations

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• polymer induced a reduction by 30% and 40%, respectively, which is absent for the carboxy-terminated polymer. Furthermore, interface-sensitive impedance spectroscopy (electric cell–substrate impedance sensing, ECIS) was employed in order to investigate the micromotility of cells added to substrates

• properties of the coating agent and its reactive group. The impact on surfactant-induced cell behavior was investigated in more detail by interface-sensitive impedance spectroscopy (electric cell–substrate impedance sensing, ECIS). Studies on the uptake and influence on metabolic activity with respect to

• addition to live cell imaging, a biocompatibility test was performed based on detecting the cell shape fluctuations of subconfluent cells cultured on small gold electrodes of 250 μm diameter, the so-called micromotion assay [18][19]. Electric cell–substrate impedance sensing (ECIS) is an electrochemical

Direct monitoring of opto-mechanical switching of self-assembled monolayer films containing the azobenzene group

• Einat Tirosh,
• Enrico Benassi,
• Silvio Pipolo,
• Marcel Mayor,
• Michal Valášek,
• Veronica Frydman,
• Stefano Corni and
• Sidney R. Cohen

Beilstein J. Nanotechnol. 2011, 2, 834–844, doi:10.3762/bjnano.2.93

• ''-terphenyl)-4-yl]diazene (3-DA), such that the calculated Ecis/Etrans ratios are 2.33, 1.79 and 1.64, respectively. Clearly, the cis-configuration is stiffer than the trans for all the compounds studied. The MD model chosen to mimic the SAM is shown in Figure 6 and is fully described in the Methods section

• 1-, 2-, and 3-DA respectively. From these values, the Ecis/Etrans values reported in the main text are recovered. Finally, we also performed a test to evaluate the role of the Au–S–azobenzene bending angle in determining the stiffness ratio. In fact, in our QM model this bending is neglected. We

• problem by repeating the compressions four times, starting from four different initial conditions, and averaging the results. The ratio of the elastic moduli Ecis/Etrans is calculated considering a thickness ratio l0,cis/ l0,trans equal to 1.054 (estimated from simulations without the indenter). The