Classification and application of metal-based nanoantioxidants in medicine and healthcare

• Nguyen Nhat Nam,
• Nguyen Khoi Song Tran,
• Tan Tai Nguyen,
• Nguyen Ngoc Trai,
• Nguyen Phuong Thuy,
• Hoang Dang Khoa Do,
• Nhu Hoa Thi Tran and
• Kieu The Loan Trinh

Beilstein J. Nanotechnol. 2024, 15, 396–415, doi:10.3762/bjnano.15.36

• than copper content and size. The enzyme activity of natural CAT highly depends on the three-dimensional (3D) structure of the protein. A significant decrease or even loss of CAT activity can be caused already by small changes in the protein conformation and structure. As a matter of fact, cellular

• is caused by protein denaturation at high temperatures. Impressively, Co3O4 nanomaterials with different morphologies (nanoplates, nanorods, and nanocubes) exhibited the highest relative activity at very high pH (pH 9) and temperature (90 °C) [39]. Similar results were also reported for platinum

• lipoprotein receptor-related protein overexpressed on cells that comprise the BBB. Figure 3 illustrates the biodistribution and ROS scavenging activity of edaravone-encapsulated nanospherical albumin (EeNA) [91]. Alzheimer’s disease is a neurological disease that slowly destroys thinking skills and memory

Nanomedicines against Chagas disease: a critical review

• Maria Jose Morilla,
• Kajal Ghosal and
• Eder Lilia Romero

Beilstein J. Nanotechnol. 2024, 15, 333–349, doi:10.3762/bjnano.15.30

• , resulting in its fast killing. Lately, it was suggested that the major metabolic impact of BNZ is on the glutathione (and trypanothione) pathway so that covalent binding of BNZ with low-molecular-weight thiols and with protein thiols is the drug’s primary mode of action against T. cruzi [15]. In mammalian

• Loncastuximab tesirine, launched in 2021 to treat B-cell lymphoma [84]. Nearly 10% are polymer–drug/protein conjugates such as polyethylene glycol-ʟ-asparaginase (Calaspargase pegol, Asparlas), launched in 2019 in the USA to treat acute lymphoblastic leukemia [85]. Another 10% are protein-based nanoparticles

• including Abraxane, the first formulation based on protein nanotechnology launched in 2005 [86]. Nearly 10% are inorganic nanoparticles such as the radiosensitizer Hensify, which in 2019 obtained CE Mark approval in the European Union for the treatment of locally advanced soft tissue sarcoma. This category

Exploring the relationships between physiochemical properties of nanoparticles and cell damage to combat cancer growth using simple periodic table-based descriptors

• Joyita Roy and
• Kunal Roy

Beilstein J. Nanotechnol. 2024, 15, 297–309, doi:10.3762/bjnano.15.27

• protein corona. The formation of a protein corona on the surface of NPs, which influences the interaction with cell membranes or proteins, is also associated with zeta potential and surface charge. Very limited studies have reported the influence of zeta potential, surface charge, hydrophobicity, and

• hydroxyl radicals, resulting in oxidative damage to proteins. Moreover, they can bind non-specifically to amino acid residues and replace existing metal ions at active sites of enzymes, leading to abnormal protein folding. Protein aggregation diseases are a type of neurodegenerative diseases that occur

Multiscale modelling of biomolecular corona formation on metallic surfaces

• Parinaz Mosaddeghi Amini,
• Ian Rouse,
• Julia Subbotina and
• Vladimir Lobaskin

Beilstein J. Nanotechnol. 2024, 15, 215–229, doi:10.3762/bjnano.15.21

• modelling of the interaction between various surfaces, that is (100), (110), and (111), of fcc aluminum with the most abundant milk proteins and lactose. Our approach combines atomistic molecular dynamics, a coarse-grained model of protein adsorption, and kinetic Monte Carlo simulations to predict the

• protein corona composition in the deposited milk layer on aluminum surfaces. We consider a simplified model of milk, which is composed of the six most abundant milk proteins found in natural cow milk and lactose, which is the most abundant sugar found in dairy. Through our study, we ranked selected

• provide valuable insights into the mechanisms of lactose and protein deposition on aluminum surfaces, which can aid in the general understanding of protein corona formation. Keywords: all atomistic; aluminum; bionano interface; coarse grained model; lactose; milk protein; multiscale modelling; protein

Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics

• Phuong-Thao Dang-Luong,
• Hong-Phuc Nguyen,
• Loc Le-Tuan,
• Xuan-Thang Cao,
• Vy Tran-Anh and
• Hieu Vu Quang

Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17

• and target efficacy. To increase the blood half-life, stealth materials have been attached to the nanoparticle surface to prevent protein adsorption and immune cell phagocytosis [4]. Most sheath materials are hydrophilic polymers such as poly(vinyl alcohol), poly(ethylene glycol), and poly(ethylene

• cytoskeleton and chlorambucil (CHL) inhibits DNA synthesis. These drugs can be encapsulated inside nanoparticles for administration to increase the stability of the medication in circulation and therapeutic efficacy. For example, doxorubicin can be inserted into liposomes and paclitaxel attaches to the protein

• NPs also helps to maintain its stability. Poly(ethylene glycol) (PEG) on the surface of NPs would serve as a brush to inhibit serum protein adsorption [4]. The PEO block of F127 shares the same core structure as PEG; hence, the emergence of a form of PEG would likewise improve the pharmacokinetics of

Assessing phytotoxicity and tolerance levels of ZnO nanoparticles on Raphanus sativus: implications for widespread adoptions

• Pathirannahalage Sahan Samuditha,
• Nadeesh Madusanka Adassooriya and
• Nazeera Salim

Beilstein J. Nanotechnol. 2024, 15, 115–125, doi:10.3762/bjnano.15.11

• light scattering (DLS), and scanning electron microscopy (SEM). The effect of ZnO NPs (70 nm) on R. sativus grown in coir was evaluated. The application of 1,000 mg/L of ZnO NPs resulted in a significant increase (p < 0.05) in soluble protein content, carbohydrates, chlorophyll a (Chl-a), chlorophyll b

• (p < 0.05) in soluble protein content by 23.1%, accompanied by a notable increase in IAA by 31.1%, indicating potential toxicity. The use of atomic absorption spectroscopy confirmed the internalization of zinc in seedlings, with a statistically significant increase (p < 0.05). In control plants

• at higher doses was investigated against R. sativus to determine its tolerance. Further, this study also attempted to assess the accumulation of Zn in R. sativus seedlings by determining the effect of ZnO NPs on the soluble protein content, indole acetic acid (IAA) content, total carbohydrate content

Study of the reusability and stability of nylon nanofibres as an antibody immobilisation surface

• Inés Peraile,
• Matilde Gil-García,
• Laura González-López,
• Nushin A. Dabbagh-Escalante,
• Juan C. Cabria-Ramos and
• Paloma Lorenzo-Lozano

Beilstein J. Nanotechnol. 2024, 15, 83–94, doi:10.3762/bjnano.15.8

• nanofibres with well-oriented antibodies anchored by protein A/G. Our study shows that stripping with glycine buffer pH 2.5 allows the nanofibres to be reused as long as protein A/G has been previously anchored, leaving both nanofibre and protein A/G unchanged. On the other hand, we investigated the

• stability of the nylon nanofibres. To achieve this, we analysed any loss of immunocapture ability of well-oriented antibodies anchored both to the nylon nanofibres and to a specialised surface with high protein binding capacity. The nanofibre immunocapture system maintained an unchanged immunocapture

• reusability study”. Ricin has been chosen as a representative biotoxin because it has been used in biological warfare attacks because of its high toxicity, stability, and availability. It belongs to the ribosome-inactivating protein family and causes cell death by disrupting protein synthesis [20]. Results

Nanotechnological approaches in the treatment of schistosomiasis: an overview

• Lucas Carvalho,
• Michelle Sarcinelli and
• Beatriz Patrício

Beilstein J. Nanotechnol. 2024, 15, 13–25, doi:10.3762/bjnano.15.2

• , Adekiya et al. [40] produced PZQ encapsulated in nanoliposomes whose surface was modified with an antibody against calpain, a protein found in the tegument of the parasite and is upregulated in the regions where host–parasite interaction occurs [41]. The modified nanoparticles orally administered two or

Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells

• Eloïse Equy,
• Jordana Hirtzel,
• Sophie Hellé,
• Béatrice Heurtault,
• Eric Mathieu,
• Morgane Rabineau,
• Vincent Ball and
• Lydie Ploux

Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100

• applications, especially because of their biocompatibility. We synthesized and characterized fluorescent PDA NPs of 10–25 nm diameter based on a protein containing a lysine–glutamate diad (bovine serum albumin, BSA) and determined whether they can penetrate and accumulate in bacterial cells to serve as a

• additive plays a crucial role in the control of the NP size. Specifically, Bergtold et al. demonstrated that a protein (e.g. chromofungin) containing a diad of lysine (K) and glutamate (E) (Figure 1a,b) in its sequence allows for the control of the formation of PDA NPs, in contrast to an additive without a

• aromatic ring of dopamine (Figure 1c). For this, K and E residues must be next to each other. Even a single glycine residue (G) located between K and E can destabilize the aggregates [13]. Among such possible additives, the albumin protein is an interesting candidate since it contains one KE diad and is

Hierarchically patterned polyurethane microgrooves featuring nanopillars or nanoholes for neurite elongation and alignment

• Lester Uy Vinzons,
• Guo-Chung Dong and
• Shu-Ping Lin

Beilstein J. Nanotechnol. 2023, 14, 1157–1168, doi:10.3762/bjnano.14.96

• had a rounded bottom. The space between the pillars and holes were around 860 nm and 330 nm, respectively. The wettability of a surface is a good predictor of protein adsorption and bioactivity [20]. For the extracellular matrix protein laminin, good adsorption and cell growth have been found on

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• stability and solubility [9]. The complexation occurs mainly through hydrophobic interactions in protein cavities [10][11]. In a recent study, zein nanoparticles loaded with CUR have been studied for their potential in treating brain tumors, and the results have demonstrated a reduction in the proliferation

• 1644 and 1546 cm−1 indicated the vibration adsorption of amide I (C=O stretching) and amide II (C–N stretching and N–H bending vibrations), respectively. These are the main vibrational bands in the albumin backbone that formed the secondary structure of the protein. It is also seen that the absorption

• peaks of HSA-MPs (orange line spectrum) have almost the same characteristic peaks of HSA, including amide I and amide II. Interestingly, the similarity between the absorption peaks of HSA-MPs and CUR-HSA-MPs (red line spectrum) suggests that CUR encapsulation does not significantly alter the protein

Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one

• Jonathan-Gabriel Nimz,
• Pichayut Rerkshanandana,
• Chiraphat Kloypan,
• Ulrich Kalus,
• Saranya Chaiwaree,
• Axel Pruß,
• Radostina Georgieva,
• Yu Xiong and
• Hans Bäumler

Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85

• occur in the liver [24][25][26]. The question of the mechanisms by which HBOCs are sequestered remains partly unclear though. Possible degradation pathways include haptoglobin (Hp), which, depending on the size and surface properties of HBOCs, could bind its physiological target protein hemoglobin [16

• site within the Hb α chain is freely accessible (exclusively β-crosslinked Hb vs α-crosslinked Hb) [27]. Intermolecular modifications changing the molecular or polymer size of HBOCs [16][19][30] are relevant for protein binding as well. Hemoglobin sub-micron particles (HbMPs) obtained via

• since both proteins are specific for monocytes. CD163 was tested because of its direct affinity to Hb. Also, we tested the effect of blocking CD204 (scavenger receptor A/SR-A). SR-A is a membrane protein occurring in the monocyte/macrophage lineage. Playing an important role in host defense, it exhibits

Nanoarchitectonics of photothermal materials to enhance the sensitivity of lateral flow assays

• Elangovan Sarathkumar,
• Rajasekharan S. Anjana and
• Ramapurath S. Jayasree

Beilstein J. Nanotechnol. 2023, 14, 988–1003, doi:10.3762/bjnano.14.82

• 2017 to determine the impact of GNP size on LFA performance [25]. They compared three different sizes of GNPs (30, 60, and 100 nm) in order to determine both the colorimetric and photothermal effect on the LFA membrane (Figure 7A). The group considered C-reactive protein as a model antigen, and the

Antibody-conjugated nanoparticles for target-specific drug delivery of chemotherapeutics

• Mamta Kumari,
• Amitabha Acharya and
• Praveen Thaggikuppe Krishnamurthy

Beilstein J. Nanotechnol. 2023, 14, 912–926, doi:10.3762/bjnano.14.75

• chemotherapeutic agent in a controlled manner. Appropriately designed and synthesized ACNPs are essential to fully realize their therapeutic benefits. In blood stream, ACNPs instantly interact with biological molecules, and a protein corona is formed. Protein corona formation triggers an immune response and

• affects the targeting ability of the nanoformulation. In this review, we provide recent findings to highlight several antibody conjugation methods such as adsorption, covalent conjugation, and biotin–avidin interaction. This review also provides an overview of the many effects of the protein corona and

• fragment antigen-binding (Fab) region of mAbs are chemically conjugated to NP surfaces to recognize protein targets that are overexpressed on the surface of tumor cells. Conjugation of mAbs to NP surfaces improves targeting capacity, cellular uptake, and intracellular stability [12]. The mAb-functionalized

Biomimetics on the micro- and nanoscale – The 25th anniversary of the lotus effect

• Matthias Mail,
• Kerstin Koch,
• Thomas Speck,
• William M. Megill and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2023, 14, 850–856, doi:10.3762/bjnano.14.69

• paper “Bioselectivity of silk protein-based materials and their bio-inspired applications” the importance of tailoring bioselective, biologically active, and multifunctional materials for biomedical applications in biomaterial research. The review was focused on two major topics, the first one being

• biological processes and surface interactions involved in the bioselective adhesion of mammalian cells. The second topic of the review was on repellence of microbes on protein-based material surfaces, highlighting the importance of materials made of recombinant spider silk proteins. Biomaterials that

• the same time selectively enhance regeneration in host tissues. The authors point out that in this context, protein-based materials and especially silk materials are interesting candidates due to their natural origin, biological activity, and structural properties. These exciting recombinant

Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies

• Giuliana Muraca,
• María Esperanza Ruiz,
• Rocío C. Gambaro,
• Sebastián Scioli-Montoto,
• María Laura Sbaraglini,
• Gisel Padula,
• José Sebastián Cisneros,
• Cecilia Yamil Chain,
• Vera A. Álvarez,
• Cristián Huck-Iriart,
• Guillermo R. Castro,
• María Belén Piñero,
• Matias Ildebrando Marchetto,
• Catalina Alba Soto,
• Germán A. Islan and
• Alan Talevi

Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66

• intrinsic toxicity of our nanoscale vehicle on T. cruzi may be linked to a modification of glycosylphosphatidylinositols (GPIs). Glycosylphosphatidylinositols are the main anchor complexes used by protozoans to bind to cell surface proteins. It covalently attaches to the C terminus of a protein connecting

• it to the outer leaflet of the lipid bilayer [49]. Trypanosoma brucei predominant membrane protein variant surface glycoprotein (VSG), which is involved in parasite host immune system evasion, is anchored by a GPI that requires myristate for its synthesis. Analogs of myristate have shown toxicity

Suspension feeding in Copepoda (Crustacea) – a numerical model of setae acting in concert

• Alexander E. Filippov,
• Wencke Krings and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2023, 14, 603–615, doi:10.3762/bjnano.14.50

• cuticle’s mechanical properties revealed that the setae on maxillae 1 (long setae) and 2 (short setae) possess very soft bases full of the elastic protein resilin [55][56][57]. Additionally, the tips of the short setae on maxilla 2 exhibited a blue autofluorescence signal, which strongly indicated that

The steep road to nonviral nanomedicines: Frequent challenges and culprits in designing nanoparticles for gene therapy

• Yao Yao,
• Yeongun Ko,
• Grant Grasman,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2023, 14, 351–361, doi:10.3762/bjnano.14.30

• are assessed with the use of fluorescent-labeling carriers and the expression of fluorescent proteins (e.g., enhanced green fluorescent protein). Both of which are typically assessed by widefield fluorescent microscopy/confocal microscopy (referred to as “imaging”) and/or flow cytometry (Table 1

• pharmacological inhibitors. However, genetic alterations may also result in changes that share protein components or lead to compensatory mechanisms in the cell [37]. Despite their intrinsic specificity, validation still remains critical to avoid affecting multiple pathways. Hence, the inclusion of appropriate

• polymeric (e.g., core–shell micelles), oligomeric (e.g., lipid nanoparticles), or biomacromolecular (e.g., protein nanoparticles) components complicates matters only further by generating a higher-than-normal background through non-specific interactions with the assay media. In addition, a significant bias

Polymer nanoparticles from low-energy nanoemulsions for biomedical applications

• Santiago Grijalvo and
• Carlos Rodriguez-Abreu

Beilstein J. Nanotechnol. 2023, 14, 339–350, doi:10.3762/bjnano.14.29

•  5) with viabilities higher than 70% for HeLa cells are promising candidates for gene therapy (e.g., gene vaccines). Protein binding on PLGA nanoparticles prepared from nanoemulsions has also been studied [62]. After incubation with human serum, afamin was one of the specific proteins bound to PLGA

• encapsulated in PLGA nanoparticles derived from PIC nanoemulsions [63]. These antioxidant-loaded nanoparticles feature hydrodynamic sizes between 71 and 160 nm and encapsulation efficiencies higher than 64%. The colloidal stability of the nanoparticle dispersions was not significantly affected by protein

Overview of mechanism and consequences of endothelial leakiness caused by metal and polymeric nanoparticles

• Magdalena Lasak and
• Karol Ciepluch

Beilstein J. Nanotechnol. 2023, 14, 329–338, doi:10.3762/bjnano.14.28

• nutrient molecules to NPs could change the toxicity of NPs (NP protein corona), and the physiological conditions, such as blood flow and physiological stretch, will also play a role [37][38][39]. NanoEL mechanism Adherens junctions between endothelial cells are maintained by a complex set of proteins

• physicochemical properties of NPs, one must also understand the mechanism behind NanoEL. The crucial molecular target of NPs was confirmed to be VE-cad, a protein essential for maintaining the structural and functional integrity of the endothelium [40]. As reported by Setyawati et al., TiO2 NPs disrupt homophilic

Biocatalytic synthesis and ordered self-assembly of silica nanoparticles via a silica-binding peptide

• Mustafa Gungormus

Beilstein J. Nanotechnol. 2023, 14, 280–290, doi:10.3762/bjnano.14.25

• stabilizes the favorable orientation of histidine. Then a nucleophilic attack by serine on the Si–O bond of the precursor molecule results in a Ser–O–Si(OR)3 transitory complex. The hydrolysis is completed by the addition of water, separating the protein and the hydrolyzed precursor molecule, and the release

Recent progress in cancer cell membrane-based nanoparticles for biomedical applications

• Qixiong Lin,
• Yueyou Peng,
• Yanyan Wen,
• Xiaoqiong Li,
• Donglian Du,
• Weibin Dai,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2023, 14, 262–279, doi:10.3762/bjnano.14.24

• system (RES) or the mononuclear phagocytosis system (MPS) [4]. The subsequent rapid clearance from blood circulation by the liver and kidneys results in insufficient drug accumulation in the target tissue [5]. In addition, NPs can interact with proteins to form a protein corona, which affects the

• intended function of the NPs, resulting in changes of biological behavior and loss of function [6][7]. Moreover, the protein corona can accelerate RES/MPS uptake and interfere with the targeting ability of NPs [8]. The biomimetic technique of cell membrane coating, which employs naturally cell-derived

• interact with other cells. The biological effects of nanoformulations can be enhanced through the effective utilization of specific protein groups. A schematic diagram of surface proteins and functions of the cancer cell membrane is shown in Figure 2. 2.1 Homologous targeting Cancer cells usually exhibit

Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer

• Filip Gorachinov,
• Fatima Mraiche,
• Diala Alhaj Moustafa,
• Ola Hishari,
• Yomna Ismail,
• Jensa Joseph,
• Maja Simonoska Crcarevska,
• Marija Glavas Dodov,
• Nikola Geskovski and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23

• . Along with the well-established EGFR, Kirsten rat sarcoma viral oncogene homolog (KRAS) oncogene mutations and concurrent anaplastic lymphoma kinase (ALK) and proto-oncogene tyrosine-protein kinase (ROS1) rearrangements, other gene mutations in the context of NSCLC tumorigenesis biomarkers and targets

• for new clinical therapies include fusions of echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (EML4-ALK) and mutations of human epidermal growth factor receptor 2 (HER2), phosphatidylinositol 3-kinase (PIK3CA), protein kinase B (AKT), v-raf murine sarcoma viral oncogene

• homolog B1 (BRAF), mitogen-activated protein kinase 1 (MAP2K1), and mesenchymal-epithelial transition factor (MET). An improved understanding of EGFR driver mutations leads the way to the establishment of personalized clinical therapy based on genomic and proteomic testing, which is becoming a standard of

Cyclodextrins as eminent constituents in nanoarchitectonics for drug delivery systems

• Makoto Komiyama

Beilstein J. Nanotechnol. 2023, 14, 218–232, doi:10.3762/bjnano.14.21

• siRNA from the endosome. In another example, a supramolecular nanoparticle was prepared from a linear CyD-based polymer, hydrophilic polyethylene glycol bearing an adamantane at the end, and siRNA [64]. By attaching a human transferrin protein, this composite was steered to target cancer cells to

• transferrin (tumor-targeting protein) which bears poly(ʟ-lysine), mitochondrion-targeting peptide, poly(ethylene glycol), and arylazopyrazole (trans isomer) [89]. Under irradiation with NIR light (808 nm), the photothermal effect disrupted mitochondrial function, leading to inhibition of tumor growth. 6 Some

• also to increase the solubility of the protein through the binding to bulky and apolar side chains. The antiviral activity of modified CyDs towards various viruses has been previously reported as well [108]. 6.2 Molecular imprinting on CyD to bind large drugs It often happens that one CyD molecule is

Antimicrobial and mechanical properties of functionalized textile by nanoarchitectured photoinduced Ag@polymer coating

• Jessica Plé,
• Marine Dabert,
• Helene Lecoq,
• Sophie Hellé,
• Lydie Ploux and
• Lavinia Balan

Beilstein J. Nanotechnol. 2023, 14, 95–109, doi:10.3762/bjnano.14.11

• with compounds generating reactive oxygen species (ROS) or blocks DNA replication and protein action (enzymes), thereby leading to cell death. The increased growth inhibition observed for C. albicans compared to E. coli could be explained in part by the difference in kinetics of silver penetration into