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Beilstein Organic Chemistry Symposium 2014

 
 

Studies toward bivalent κ opioids derived from salvinorin A: heteromethylation of the furan ring reduces affinity

Thomas A. Munro1,2, Wei Xu3, Douglas M. Ho4, Lee-Yuan Liu-Chen3 and Bruce M. Cohen1
1McLean Hospital, Belmont, MA 02478, USA and Department of Psychiatry, Harvard Medical School, Boston, MA 02215, USA
2School of Chemistry and Bio21 Institute, University of Melbourne, Parkville 3010, Australia
3Center for Substance Abuse Research and Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
4Department of Chemistry and Chemical Biology, Harvard University, Cambridge MA 02138, USA
Email of corresponding author Corresponding author email     
This article is part of the Thematic Series "Natural products in synthesis and biosynthesis".
Guest Editor: J. S. Dickschat
Beilstein J. Org. Chem. 2013, 9, 2916–2924.
doi:10.3762/bjoc.9.328
 
 
 
 

Abstract

The recent crystal structure of the κ-opioid receptor (κ-OR) revealed, unexpectedly, that the antagonist JDTic is a bivalent ligand: in addition to the orthosteric pocket occupied by morphinans, JDTic also occupies a distinct (allotopic) pocket. Mutagenesis data suggest that salvinorin A (1) also binds to this allotopic pocket, adjacent to the aspartate residue that anchors the basic nitrogen atom of classical opiates (Asp138). It has been suggested that an H-bond donor appended to 1 might interact with Asp138, increasing affinity. Such a bivalent ligand might also possess altered functional selectivity. Based on modeling and known N-furanylmethyl opioid antagonists, we appended H-bond donors to the furan ring of 1. (Dimethylamino)methyl groups at C-15 or C-16 abolished affinity for κ-OR. Hydroxymethylation at C-16 was tolerated, but 15,16-bis-hydroxymethylation was not. Since allosteric modulators may go undetected in binding assays, we also tested these and other low-affinity derivatives of 1 for allosteric modulation of dynorphin A in the [35S]GTPγS assay. No modulation was detected. As an alternative attachment point for bivalent derivatives, we prepared the 2-(hydroxyethoxy)methyl ether, which retained high affinity for κ-OR. We discuss alternative design strategies for linked, fused or merged bivalent derivatives of 1.

Keywords: allotopic; bivalent ligand; designed multiple ligand; JDTic; κ-opioid receptor; natural products; Salvinorin A

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Introduction

The structure–activity relationships of salvinorin A (1), a potent and selective κ (kappa) opioid, have been extensively investigated [1]. Modifications at C-2 have yielded compounds with increased potency and duration of action [2,3], and other compounds with unprecedented functional selectivity at the μ (mu) opioid receptor (μ-OR) [4]. However, modifications at other positions substantially reduce or eliminate affinity for opioid receptors [1]. In particular, most modifications of the furan ring tested to date dramatically reduce affinity for κ-OR [1], although small substituents at C-16 have little effect (Figure 1). Unexpectedly, epimerization at C-12, inverting the configuration of the furan ring, reduces efficacy in G-protein activation [5], but not in arrestin recruitment, resulting in functional selectivity (biased agonism) [6].

[1860-5397-9-328-1]
Figure 1: Binding affinities of salvinorin A (1) and furan derivatives for κ-OR [5].

Extensive receptor mutagenesis data are available on the interactions of κ-OR with 1 and derivatives [1,7-9]. In Figure 2A, the crystal structure of κ-OR in complex with the selective antagonist JDTic is shown. Essential residues for high affinity binding of 1, where mutation reproducibly reduces affinity ≥10-fold, are shown with thick bonds for emphasis [7-9]. These key residues are all located in transmembrane helices (TMs) 2 and 7. The surfaces of these residues are contiguous, forming one face of a deep pocket between TMs 2, 3, and 7. Mutations of many other residues outside this pocket (TMs 1, 3, 5, 6, 7, and extracellular loop 2) failed to substantially reduce affinity for 1 [1,7-9]. Collectively, these results provide compelling evidence that 1 binds to this face of the binding pocket. The plausibility of this proposal is strengthened by the position of the hydroxyphenylpiperidine moiety of JDTic in the crystal structure (HPP, light blue). The HPP moiety and its N-substituent interact with five of the seven key residues for binding of 1: Val1082.53, Gln1152.60, Val1182.63, Ile3167.39, and Tyr3207.43 (superscripts refer to Ballesteros–Weinstein numbering) [7]. Intriguingly, 1 contains a substructure very similar to HPP (light blue in Figure 2B). Given that HPP is a near substructure of 1 and binds to these same key residues, it is tempting to speculate that the binding pose of 1 may be similar.

[1860-5397-9-328-2]
Figure 2: Crystal structure of κ-OR in complex with JDTic compared to naltrindole’s binding pose in δ-OR. A: Cross-eyed stereoview of the crystal structure of κ-OR (PDB 4DJH). All residues known to be required for high-affinity binding of 1 (mutation reproducibly reduces affinity ≥10-fold [7-9]) are shown with thick bonds and water-accessible surfaces. JDTic is shown in green with the hydroxyphenylpiperidine substructure (HPP) in light blue. Ionic H-bonds from JDTic to Asp1383.32 are shown in red. The binding pose of naltrindole (NTI, pink) to δ-OR is also superimposed (PDB 4EJ4). B: Structural similarities: atoms in 1 common to the HPP substructure of JDTic are shown in light blue; superimposable portions of naltrindole and JDTic are shown in pink.

By contrast, the morphinan naltrindole (shown in pink) binds to a different pocket of δ-OR among TMs 3, 6, and 7 [10]; β-funaltrexamine adopts a near-identical pose in complex with μ-OR (not shown) [11]. Interestingly, JDTic occupies this morphinan pocket as well. The tetrahydroisoquinoline moiety of JDTic adopts a pose almost identical to that of naltrindole; the superimposable atoms are shown in pink in Figure 2B [10]. JDTic is thus a bivalent ligand, containing two linked pharmacophores [12], also known as a linked multiple ligand [13]. Bivalent ligands can exhibit greatly increased potency and unusual functional selectivity [12]. Consistent with this, JDTic is extremely potent (Ke < 40 pM) [14]; also, despite acting as an antagonist toward other κ-OR-mediated signaling pathways [7], JDTic reportedly activates JNK1 (MAPK8), causing extremely prolonged desensitization of κ-OR [15].

These two binding pockets are almost entirely separate, but overlap around Tyr3207.43. This residue interacts with JDTic [7], and the corresponding residue (Tyr7.43) also interacts with naltrindole in δ-OR [10] and β-funaltrexamine in μ-OR [11].

Interestingly, Tyr3207.43 and two adjacent residues (Val1082.53 and Ile3167.39) are also key residues for the binding of 1 [7]. Together, they surround the bottom of the binding pocket, strongly suggesting that an extremity of 1 occupies this space, overlapping with both the morphinan and hydroxyphenylpiperidine binding sites.

Immediately adjacent to these residues is Asp1383.32, a critical residue for binding of morphinans and related opioids. Asp3.32 forms strong ionic H-bonds (‘salt bridges’) to the basic nitrogen atoms found in almost all opioids, as in the crystal structures of JDTic (red in Figure 2A), naltrindole [10], β-funaltrexamine [11], and the NOP antagonist C-24 [16]. Beyond opioids, ionic H-bonds to Asp3.32 are conserved across biogenic amine receptors; indeed, this residue3.32 interacts with the ligand in almost all GPCR crystal structures reported to date [17].

In summary, 1 appears to bind adjacent to Asp1383.32, but not to interact with it strongly: mutation of this residue either has little effect or actually increases affinity [7-9]. Based on this proximity, Kane proposed appending a positively-charged moiety to 1, thereby creating an ionic H bond to Asp1383.32 that should increase affinity [18]. Like JDTic, this ligand would be bivalent. Kane also proposed linking to fragments of other κ-opioids such as arylacetamides, creating additional interactions. By this strategy, “designed multiple ligands” of several kinds could be obtained: linked, fused or merged [13].

A suitable attachment point and linker for this second moiety would also be required. Docking 1 to an early rhodopsin-based homology model of κ-OR, Kane proposed a binding pose qualitatively similar to that of the HPP moiety of JDTic, placing the furan ring near Tyr3207.43 [8,18]. Indeed, at the time we commenced our work, all published mutagenesis-based models placed the furan ring in contact with Tyr3207.43 [1]. This interaction is plausible in light of the structure–activity relationships of opioid antagonists. In JDTic, the N-substituent of the HPP moiety interacts with Tyr3207.43 [7], and N-furanylalkyl substitution of this scaffold yields potent opioid antagonists (Figure 3) [19]. Likewise, the N-substituents of naltrindole and β-funaltrexamine also interact with Tyr7.43 [10,11], and N-furanylmethyl substitution of morphinans and related scaffolds consistently yields potent opioid antagonists [20]. These convergent results firmly establish that residues adjacent to Asp3.32, including Tyr7.43, interact favorably with furan substituents; thus, models placing the furan ring of 1 in this region are plausible. The region appears to be quite tolerant to the position, orientation and substitution of the furan ring [20].

[1860-5397-9-328-3]
Figure 3: Previously reported N-furanylalkyl opioid antagonists [19,20].

We therefore selected the furan ring as an attachment point for initial explorations of Kane’s bivalent ligand concept. Given the tolerance of 1 to modification at C-16 noted above, we chose to append H-bond donors (amino and hydroxy groups) at this position. Based on previous high-affinity ligands with a furanylmethyl substituent (Figure 3), we decided to use a short linker.

As an alternative approach, bivalent ligands with much longer linkers can bind simultaneously to both protomers in a receptor dimer, allowing selective targeting of specific receptor oligomers [21]. We also sought to provide a suitable attachment point for such linkers based on our previous studies of C-2 alkoxymethyl ethers [22,23], which have higher tolerance to alkyl chain extension [24] than other derivatives of 1 [1].

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Results

Synthesis

Initial attempts to form the 16-dimethylaminomethyl derivative 2 by treatment of 1 with dimethylamine and paraformaldehyde in acetic acid [25] gave no desired product. However, N,N-dimethylmethyleneiminium chloride [26] in dimethylformamide gave 2 and the 15-regioisomer 3 in good total yield (Scheme 1). Treatment of 2 with HCl in Et2O gave the hydrochloride salt, but the same treatment of 3 gave a cloudy suspension, with decomposition products evident in the 1H NMR spectrum.

[1860-5397-9-328-i1]
Scheme 1: Syntheses of heteromethylated derivatives of 1. b.r.s.m. = based on recovered starting material.

We next targeted hydroxymethyl substituents. Treatment of 1 with aqueous formaldehyde (40% w/v) and Amberlyst-15® resin at room temperature [27] gave complex mixtures of products. However, heating 1 with paraformaldehyde in acetic acid [28] gave 4 and 5, albeit in low yields based on recovered starting material (b.r.s.m.) (Scheme 1). Prolonging the reaction or raising temperatures did not increase yields. No 15-hydroxymethyl derivative was isolated. Excess paraformaldehyde tended to precipitate during chromatography, blocking the column. This was therefore extracted from the crude product with hot water. Our results conflict with a previous report that heating 1 in acetic acid led to deacetylation and epimerization [29]. In our hands, 1 was freely soluble in glacial acetic acid and was recovered unchanged after heating overnight at reflux.

As an alternative linker attachment point, (2-hydroxyethoxy)methyl ether 6 was prepared from the known methylthiomethyl ether 7 using a slight modification of previous conditions (Scheme 2) [24].

[1860-5397-9-328-i2]
Scheme 2: Synthesis of (2-hydroxyethoxy)methyl ether 6.

Structure elucidation

The molecular formula of 2 and 3 was established by HRMS, confirming the addition of a dimethylaminomethyl group in each case. The structure of 2 was established by X-ray crystallography (Figure 4) after recrystallization from ethanol/hexanes.

[1860-5397-9-328-4]
Figure 4: Crystal structure of 2 with 50% probability thermal displacement ellipsoids (cross-eyed stereoview). For clarity, only one of the two observed orientations for the disordered dimethylamino group is shown. For the other orientation and partially-occupied ethanol solvate, see Supporting Information File 1, Figure S1. For coordinates, see Supporting Information File 3 or CCDC 970639.

Consistent with the crystal structure, comparison of the 1H NMR spectrum of 2 with the fully assigned spectrum of 1 [30,31] revealed new dimethylamino (δ 2.22, s, 6H) and aminomethylene (δ ~3.4, m, 2H) signals and the loss of one furan oxymethine. The remaining oxymethine signal (δ 7.31) was a doublet with a typical vicinal coupling constant (1.7 Hz) to H-14 (δ 6.28), confirming that they were adjacent. The multiple-bond 1H–13C (HMBC) correlations for 2 shown in Figure 5 confirm substitution at C-16.

[1860-5397-9-328-5]
Figure 5: Key 1H–13C HMBC correlations.

The structure of the other regioisomer, 3, was confirmed using NMR data. The oxymethine signal (δ 7.34) showed only long-range couplings (J = 0.8 Hz), confirming that it was isolated. The HMBC correlations (Figure 5) confirmed substitution at C-15.

The molecular formula of 4, established by HRMS, confirmed the addition of a hydroxymethyl group. Like the C-16-substituted 4, the two furan peaks in the 1H NMR spectrum were doublets with a typical vicinal coupling constant (1.9 Hz). The same HMBC correlations were observed as for 2 (Figure 5), confirming substitution at C-16. The molecular formula of 5, established by HRMS, indicated the addition of two hydroxymethyl groups. The remaining furan oxymethine signal seen in the 1H NMR spectrum of 4 was lost, leaving an H-14 singlet (δ 6.21), confirming substitution at C-15 and C-16. An oxymethylene triplet (δ 4.61, t, 2H) and doublet (δ 4.55, d, 2H) collapsed to a doublet and singlet, respectively, after washing with D2O.

The molecular formula of (2-hydroxyethoxy)methyl ether 6 was confirmed by HRMS. The structure was confirmed by comparison of 1H and 13C NMR spectra with the previously reported (2-methoxyethoxy)methyl ether [24].

Bioassays

We next determined the binding affinities of these compounds in blinded tests for the human κ-OR expressed in Chinese hamster ovary cells (CHO-κ-OR). In an initial screening of the furan derivatives at 3 µM, only 4 caused ≥50% inhibition of [3H]diprenorphine binding. The Ki of 4 was 110 nM (Table 1), a substantial reduction in affinity relative to 1. None of these compounds showed detectable affinity for µ- or δ-OR (Ki > 1 μM in all cases). Thus, 4 showed modest affinity and selectivity for κ-OR, while 2, 3 and 5 showed no affinity for any opioid receptor. Follow-up testing in the [35S]GTPγS functional assay demonstrated that 4 was an agonist less potent than 1 at κ-OR, but giving an equal maximal response (Table 1). (2-Hydroxyethoxy)methyl ether 6 showed high affinity for κ-OR, but not for µ- or δ-OR (Ki > 1 μM), similar to other alkoxymethyl ethers [23,24].

Table 1: Binding affinity, potency and maximal response at κ-OR.
 Ki ± SEMaEC50 ± SEMbEmaxc
 RnMnM%
[Graphic 1][Graphic 2]13.0d7.6 ± 0.3106
[Graphic 3]2>1,000  
[Graphic 4]3>1,000  
[Graphic 5]4110 ± 10345 ± 47103
[Graphic 6]5>1,000  
[Graphic 7] 612.7 ± 1.3  
 U50,488H 1.2 ± 0.26.8 ± 1.1100

Despite the low affinities and potencies of the furan derivatives, we undertook further investigations. As discussed in the Introduction, the key residues for the binding of 1 are almost entirely separate from the morphinan binding pocket. Although it seems likely that the two pockets overlap, it is possible that they are separate, or allotopic [32]. If so, 1 could bind simultaneously with morphinans and act as an allosteric modulator. Indeed, it has been reported that 1 is a low-affinity allosteric modulator of µ-OR [33]. Since it is possible for allosteric ligands to bind, and to modulate signaling, without displacing orthosteric ligands such as diprenorphine [34], binding assays can give false negative results.

We therefore tested whether the furan derivatives of 1 that failed to displace [3H]diprenorphine from κ-OR nonetheless modulated signaling. Given our interest in the development of κ-opioid antagonists [35], we tested for negative modulation. To maximize the physiological relevance of the assays, we used the endogenous agonist dynorphin A. In addition to 25, we retested a number of derivatives of 1 we had previously found to exhibit negligible affinity for κ-OR; these featured modifications at C-12 [5], C-4 [36] and C-1 [31] (Figure 6).

[1860-5397-9-328-6]
Figure 6: Known low-affinity derivatives of 1 screened in addition to 25 for negative allosteric modulation of dynorphin A at κ-OR in the [35S]GTPγS assay. No compound caused detectable antagonism at 3 μM.

In a primary screen at 3 μM, none of the compounds tested caused detectable antagonism of dynorphin A (10 nM) in the [35S]GTPγS assay (Supporting Information File 1, Table S1). By comparison, the positive control, JDTic, abolished response to dynorphin A. These results reveal no evidence of allosteric modulation, but do not exclude the possibility [34].

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Discussion

While the affinities of all these furan derivatives were low, they may nonetheless provide starting points for the development of bivalent derivatives. Installation of a leaving group on hydroxymethyl compound 4, for instance, would permit coupling with diverse N-dealkylated opiate scaffolds. Alternative attachment points would also be worth exploring, such as the C-17 lactone, which tolerates reduction and alkylation [1]. As noted in the Introduction, there is compelling evidence that 1 binds adjacent to Asp1383.32, but the involvement of the furan ring is based on modeling rather than direct evidence, and is thus highly speculative. A subsequent model for the binding of 1 derivative RB-64 places the C-18 methyl ester rather than the furan ring close to Asp1383.32 [7]. In our previous studies, however, substitution of amine groups for the C-18 methyl ester abolished affinity for κ-OR [36]. Indeed, all modifications of this group tested to date greatly reduce affinity, in most cases abolishing binding altogether [1]. C-18 is thus not a promising attachment point. At the opposite extreme, the C-2 alkoxymethyl ether series is highly tolerant to alkyl chain extension. Compound 6 and the synthetic route used may prove useful for the attachment of very long linkers, as used with other scaffolds to bridge receptor dimers [21].

Rather than linking pharmacophores, another approach to bivalent ligands is to design hybrid structures, or merged multiple ligands [13]. As noted above, the HPP moiety of JDTic interacts with many of the key residues for binding of 1, and shares a very similar substructure, suggesting that the binding pose of 1 may be similar. If so, converting the lactone to a cyclic amine may provide the desired H-bond to Asp1383.32 without the need for additional functional groups.

Our results provide no evidence of negative allosteric modulation of κ-OR by low-affinity derivatives of 1, and the mutagenesis results discussed in the Introduction suggest some overlap with other opioids. However, given the report that 1 is an allosteric modulator of μ-OR [33], high-affinity μ-selective derivatives such as herkinorin [1] also represent potential scaffolds for bivalent and possibly bitopic (orthosteric–allosteric) derivatives [12].

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Conclusion

In conclusion, compelling evidence suggests that 1 binds to an allotopic pocket of κ-OR, leaving the classical opiate binding site largely unoccupied. This suggests the possibility of creating bivalent derivatives by linking these scaffolds, with the potential for dramatic increases in potency and altered functional selectivity. A suitable attachment point and linker remain to be determined. Our preliminary modifications at C-2, C-15 and C-16 yielded no potent derivatives, but may prove useful as intermediates in further exploration of this concept.

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Author Contributions

T.A.M. designed and prepared the compounds, wrote the manuscript and prepared the figures. W.X. and L.Y.L.C. performed and analyzed the bioassays. D.M.H. obtained the crystal structure. B.M.C. supervised and coordinated the project.

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Competing Interests

B.M.C., L.Y.L.C., and T.A.M. hold US patents on the preparation and therapeutic use of salvinorin A derivatives (7,629,475 and 8,492,564).

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Supporting Information

Supporting Information File 1: Synthetic procedures and 1H and 13C NMR spectra of all new compounds; HMQC and HMBC spectra from Figure 5; supporting Figure S1 and Table S1.
Format: PDF   Size: 2.7 MB   Download

Supporting Information File 2: 1H and 13C NMR spectra of all new compounds in jcamp-dx format.
Format: ZIP   Size: 2.0 MB   Download

Supporting Information File 3: X-ray crystal structure of 2.
Format: CIF   Size: 39.0 KB   Download

Supporting Information File 4: Structure factors for 2.
Format: HKL   Size: 387.8 KB   Download

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Acknowledgements

Funding for this work was provided by the National Institute of Drug Abuse (P30 DA13429 to LYLC). Cécile Béguin suggested compounds for the negative modulation assay.

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References

  1. Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
  2. Hooker, J. M.; Munro, T. A.; Béguin, C.; Alexoff, D.; Shea, C.; Xu, Y.; Cohen, B. M. Neuropharmacology 2009, 57, 386–391. doi:10.1016/j.neuropharm.2009.06.044
    Return to citation in text: [1]
  3. Peet, M. M.; Baker, L. E. Behav. Pharmacol. 2011, 22, 450–457. doi:10.1097/FBP.0b013e328349fc1b
    Return to citation in text: [1]
  4. Lamb, K.; Tidgewell, K.; Simpson, D. S.; Bohn, L. M.; Prisinzano, T. E. Drug Alcohol Depend. 2012, 121, 181–188. doi:10.1016/j.drugalcdep.2011.10.026
    Return to citation in text: [1]
  5. Béguin, C.; Duncan, K. K.; Munro, T. A.; Ho, D. M.; Xu, W.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. 2009, 17, 1370–1380. doi:10.1016/j.bmc.2008.12.012
    Return to citation in text: [1] [2] [3]
  6. Béguin, C.; Potuzak, J.; Xu, W.; Liu-Chen, L.-Y.; Streicher, J. M.; Groer, C. E.; Bohn, L. M.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. Lett. 2012, 22, 1023–1026. doi:10.1016/j.bmcl.2011.11.128
    Return to citation in text: [1] [2]
  7. Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
  8. Kane, B. E.; McCurdy, C. R.; Ferguson, D. M. J. Med. Chem. 2008, 51, 1824–1830. doi:10.1021/jm701040v
    Return to citation in text: [1] [2] [3] [4] [5] [6]
  9. Vardy, E.; Mosier, P. D.; Frankowski, K. J.; Wu, H.; Katritch, V.; Westkaemper, R. B.; Aubé, J.; Stevens, R. C.; Roth, B. L. J. Biol. Chem. 2013, 288, 34470–34483. doi:10.1074/jbc.M113.515668
    Return to citation in text: [1] [2] [3] [4] [5]
  10. Granier, S.; Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Weis, W. I.; Kobilka, B. K. Nature 2012, 485, 400–404. doi:10.1038/nature11111
    Return to citation in text: [1] [2] [3] [4] [5]
  11. Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Mathiesen, J. M.; Sunahara, R. K.; Pardo, L.; Weis, W. I.; Kobilka, B. K.; Granier, S. Nature 2012, 485, 321–326. doi:10.1038/nature10954
    Return to citation in text: [1] [2] [3] [4]
  12. Valant, C.; Lane, J. R.; Sexton, P. M.; Christopoulos, A. Annu. Rev. Pharmacol. Toxicol. 2012, 52, 153–178. doi:10.1146/annurev-pharmtox-010611-134514
    Return to citation in text: [1] [2] [3]
  13. Morphy, R. J. Historical Strategies for Lead Generation. In Designing Multi-Target Drugs; Morphy, R. J.; Harris, C. J., Eds.; RSC: Cambridge, 2012; pp 111–129. doi:10.1039/9781849734912-00111
    Return to citation in text: [1] [2] [3]
  14. Béguin, C.; Cohen, B. M. Medicinal Chemistry of Kappa Opioid Receptor Antagonists. In Opiate Receptors and Antagonists: from Bench to Clinic; Dean, R. L.; Bilsky, E. J.; Negus, S. S., Eds.; Humana Press: New York, 2009; pp 99–118. doi:10.1007/978-1-59745-197-0_6
    Return to citation in text: [1]
  15. Melief, E. J.; Miyatake, M.; Carroll, F. I.; Béguin, C.; Carlezon, W. A., Jr.; Cohen, B. M.; Grimwood, S.; Mitch, C. H.; Rorick-Kehn, L.; Chavkin, C. Mol. Pharmacol. 2011, 80, 920–929. doi:10.1124/mol.111.074195
    Return to citation in text: [1]
  16. Thompson, A. A.; Liu, W.; Chun, E.; Katritch, V.; Wu, H.; Vardy, E.; Huang, X.-P.; Trapella, C.; Guerrini, R.; Calo, G.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 395–399. doi:10.1038/nature11085
    Return to citation in text: [1]
  17. Venkatakrishnan, A. J.; Deupi, X.; Lebon, G.; Tate, C. G.; Schertler, G. F.; Babu, M. M. Nature 2013, 494, 185–194. doi:10.1038/nature11896
    Return to citation in text: [1]
  18. Kane, B. E. Molecular recognition of G-protein coupled receptor ligands: Insights into salvinorin A and xanomeline. Ph.D. Thesis, University of Minnesota, 2006.
    http://gradworks.umi.com/32/38/3238362.html
    Return to citation in text: [1] [2]
  19. Zimmerman, D. M.; Leander, J. D.; Cantrell, B. E.; Reel, J. K.; Snoddy, J.; Mendelsohn, L. G.; Johnson, B. G.; Mitch, C. H. J. Med. Chem. 1993, 36, 2833–2841. doi:10.1021/jm00072a001
    Return to citation in text: [1] [2]
  20. Merz, H.; Langbein, A.; Stockhaus, K.; Walther, G.; Wick, H. Adv. Biochem. Psychopharmacol. 1974, 8, 91–107.
    Return to citation in text: [1] [2] [3]
  21. Hiller, C.; Kühhorn, J.; Gmeiner, P. J. Med. Chem. 2013, 56, 6542–6559. doi:10.1021/jm4004335
    Return to citation in text: [1] [2]
  22. Munro, T. A.; Ho, D. M.; Cohen, B. M. Acta Crystallogr., Sect. E: Struct. Rep. Online 2012, 68, o3225–o3226. doi:10.1107/S1600536812043449
    Return to citation in text: [1]
  23. Wang, Y.; Chen, Y.; Xu, W.; Lee, D. Y. W.; Ma, Z.; Rawls, S. M.; Cowan, A.; Liu-Chen, L. Y. J. Pharmacol. Exp. Ther. 2008, 324, 1073–1083. doi:10.1124/jpet.107.132142
    Return to citation in text: [1] [2]
  24. Munro, T. A.; Duncan, K. K.; Xu, W.; Wang, Y.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M.; Béguin, C. Bioorg. Med. Chem. 2008, 16, 1279–1286. doi:10.1016/j.bmc.2007.10.067
    Return to citation in text: [1] [2] [3] [4]
  25. Chernov, S. V.; Shul'ts, E. E.; Shakirov, M. M.; Tolstikov, G. A. Russ. J. Org. Chem. 2000, 36, 1455–1464.
    Return to citation in text: [1]
  26. Roman, G. Mini-Rev. Org. Chem. 2013, 10, 27–39. doi:10.2174/1570193x11310010003
    Return to citation in text: [1]
  27. Iovel, I.; Goldberg, Yu.; Shymanska, M. J. Mol. Catal. 1989, 57, 91–103. doi:10.1016/0304-5102(89)80129-4
    Return to citation in text: [1]
  28. Pevzner, L. M.; Ignat'ev, V. M. J. Org. Chem. USSR 1987, 23, 1169–1170.
    Return to citation in text: [1]
  29. Harding, W. W.; Schmidt, M.; Tidgewell, K.; Kannan, P.; Holden, K. G.; Dersch, C. M.; Rothman, R. B.; Prisinzano, T. E. Bioorg. Med. Chem. Lett. 2006, 16, 3170–3174. doi:10.1016/j.bmcl.2006.03.062
    Return to citation in text: [1]
  30. Ortega, A.; Blount, J. F.; Manchand, P. S. J. Chem. Soc., Perkin Trans. 1 1982, 2505–2508. doi:10.1039/P19820002505
    Return to citation in text: [1]
  31. Munro, T. A. The Chemistry of Salvia divinorum. Ph.D. Thesis, University of Melbourne, Australia, 2006.
    http://pqdtopen.proquest.com/#abstract?dispub=3304556
    Return to citation in text: [1] [2]
  32. Neubig, R. R.; Spedding, M.; Kenakin, T.; Christopoulos, A. Pharmacol. Rev. 2003, 55, 597–606. doi:10.1124/pr.55.4.4
    Return to citation in text: [1]
  33. Rothman, R. B.; Murphy, D. L.; Xu, H.; Godin, J. A.; Dersch, C. M.; Partilla, J. S.; Tidgewell, K.; Schmidt, M.; Prisinzano, T. E. J. Pharmacol. Exp. Ther. 2007, 320, 801–810. doi:10.1124/jpet.106.113167
    Return to citation in text: [1] [2]
  34. May, L. T.; Leach, K.; Sexton, P. M.; Christopoulos, A. Annu. Rev. Pharmacol. Toxicol. 2007, 47, 1–51. doi:10.1146/annurev.pharmtox.47.120505.105159
    Return to citation in text: [1] [2]
  35. Knoll, A. T.; Carlezon, W. A., Jr. Brain Res. 2010, 1314, 56–73. doi:10.1016/j.brainres.2009.09.074
    Return to citation in text: [1]
  36. Béguin, C.; Richards, M. R.; Li, J.-G.; Wang, Y.; Xu, W.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. Lett. 2006, 16, 4679–4685. doi:10.1016/j.bmcl.2006.05.093
    Return to citation in text: [1] [2]
Top
Figure 1: Binding affinities of salvinorin A (1) and furan derivatives for κ-OR [5]. Move  Close
Figure 2: Crystal structure of κ-OR in complex with JDTic compared to naltrindole’s binding pose in δ-OR. A: Cross-eyed stereoview of the crystal structure of κ-OR (PDB 4DJH). All residues known to be required for high-affinity binding of 1 (mutation reproducibly reduces affinity ≥10-fold [7-9]) are shown with thick bonds and water-accessible surfaces. JDTic is shown in green with the hydroxyphenylpiperidine substructure (HPP) in light blue. Ionic H-bonds from JDTic to Asp1383.32 are shown in red. The binding pose of naltrindole (NTI, pink) to δ-OR is also superimposed (PDB 4EJ4). B: Structural similarities: atoms in 1 common to the HPP substructure of JDTic are shown in light blue; superimposable portions of naltrindole and JDTic are shown in pink. Move  Close
Figure 3: Previously reported N-furanylalkyl opioid antagonists [19,20]. Move  Close
Figure 4: Crystal structure of 2 with 50% probability thermal displacement ellipsoids (cross-eyed stereoview). For clarity, only one of the two observed orientations for the disordered dimethylamino group is shown. For the other orientation and partially-occupied ethanol solvate, see Supporting Information File 1, Figure S1. For coordinates, see Supporting Information File 3 or CCDC 970639. Move  Close
Figure 5: Key 1H–13C HMBC correlations. Move  Close
Figure 6: Known low-affinity derivatives of 1 screened in addition to 25 for negative allosteric modulation of dynorphin A at κ-OR in the [35S]GTPγS assay. No compound caused detectable antagonism at 3 μM. Move  Close
Scheme 1: Syntheses of heteromethylated derivatives of 1. b.r.s.m. = based on recovered starting material. Move  Close
Scheme 2: Synthesis of (2-hydroxyethoxy)methyl ether 6. Move  Close
Table 1: Binding affinity, potency and maximal response at κ-OR. Move  Close
 Ki ± SEMaEC50 ± SEMbEmaxc
 RnMnM%
[Graphic 1][Graphic 2]13.0d7.6 ± 0.3106
[Graphic 3]2>1,000  
[Graphic 4]3>1,000  
[Graphic 5]4110 ± 10345 ± 47103
[Graphic 6]5>1,000  
[Graphic 7] 612.7 ± 1.3  
 U50,488H 1.2 ± 0.26.8 ± 1.1100
20.Merz, H.; Langbein, A.; Stockhaus, K.; Walther, G.; Wick, H. Adv. Biochem. Psychopharmacol. 1974, 8, 91–107.
Go to reference 20
24.Munro, T. A.; Duncan, K. K.; Xu, W.; Wang, Y.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M.; Béguin, C. Bioorg. Med. Chem. 2008, 16, 1279–1286. doi:10.1016/j.bmc.2007.10.067
Go to reference 24
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
Go to reference 1
21.Hiller, C.; Kühhorn, J.; Gmeiner, P. J. Med. Chem. 2013, 56, 6542–6559. doi:10.1021/jm4004335
Go to reference 21
22.Munro, T. A.; Ho, D. M.; Cohen, B. M. Acta Crystallogr., Sect. E: Struct. Rep. Online 2012, 68, o3225–o3226. doi:10.1107/S1600536812043449
23.Wang, Y.; Chen, Y.; Xu, W.; Lee, D. Y. W.; Ma, Z.; Rawls, S. M.; Cowan, A.; Liu-Chen, L. Y. J. Pharmacol. Exp. Ther. 2008, 324, 1073–1083. doi:10.1124/jpet.107.132142
Go to references 22,23
27.Iovel, I.; Goldberg, Yu.; Shymanska, M. J. Mol. Catal. 1989, 57, 91–103. doi:10.1016/0304-5102(89)80129-4
Go to reference 27
28.Pevzner, L. M.; Ignat'ev, V. M. J. Org. Chem. USSR 1987, 23, 1169–1170.
Go to reference 28
25.Chernov, S. V.; Shul'ts, E. E.; Shakirov, M. M.; Tolstikov, G. A. Russ. J. Org. Chem. 2000, 36, 1455–1464.
Go to reference 25
26.Roman, G. Mini-Rev. Org. Chem. 2013, 10, 27–39. doi:10.2174/1570193x11310010003
Go to reference 26
20.Merz, H.; Langbein, A.; Stockhaus, K.; Walther, G.; Wick, H. Adv. Biochem. Psychopharmacol. 1974, 8, 91–107.
Go to reference 20
19.Zimmerman, D. M.; Leander, J. D.; Cantrell, B. E.; Reel, J. K.; Snoddy, J.; Mendelsohn, L. G.; Johnson, B. G.; Mitch, C. H. J. Med. Chem. 1993, 36, 2833–2841. doi:10.1021/jm00072a001
20.Merz, H.; Langbein, A.; Stockhaus, K.; Walther, G.; Wick, H. Adv. Biochem. Psychopharmacol. 1974, 8, 91–107.
Go to references 19,20
5.Béguin, C.; Duncan, K. K.; Munro, T. A.; Ho, D. M.; Xu, W.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. 2009, 17, 1370–1380. doi:10.1016/j.bmc.2008.12.012
Go to reference 5
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
8.Kane, B. E.; McCurdy, C. R.; Ferguson, D. M. J. Med. Chem. 2008, 51, 1824–1830. doi:10.1021/jm701040v
9.Vardy, E.; Mosier, P. D.; Frankowski, K. J.; Wu, H.; Katritch, V.; Westkaemper, R. B.; Aubé, J.; Stevens, R. C.; Roth, B. L. J. Biol. Chem. 2013, 288, 34470–34483. doi:10.1074/jbc.M113.515668
Go to references 1,7-9
5.Béguin, C.; Duncan, K. K.; Munro, T. A.; Ho, D. M.; Xu, W.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. 2009, 17, 1370–1380. doi:10.1016/j.bmc.2008.12.012
Go to reference 5
6.Béguin, C.; Potuzak, J.; Xu, W.; Liu-Chen, L.-Y.; Streicher, J. M.; Groer, C. E.; Bohn, L. M.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. Lett. 2012, 22, 1023–1026. doi:10.1016/j.bmcl.2011.11.128
Go to reference 6
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
Go to reference 1
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
Go to reference 1
2.Hooker, J. M.; Munro, T. A.; Béguin, C.; Alexoff, D.; Shea, C.; Xu, Y.; Cohen, B. M. Neuropharmacology 2009, 57, 386–391. doi:10.1016/j.neuropharm.2009.06.044
3.Peet, M. M.; Baker, L. E. Behav. Pharmacol. 2011, 22, 450–457. doi:10.1097/FBP.0b013e328349fc1b
Go to references 2,3
4.Lamb, K.; Tidgewell, K.; Simpson, D. S.; Bohn, L. M.; Prisinzano, T. E. Drug Alcohol Depend. 2012, 121, 181–188. doi:10.1016/j.drugalcdep.2011.10.026
Go to reference 4
16.Thompson, A. A.; Liu, W.; Chun, E.; Katritch, V.; Wu, H.; Vardy, E.; Huang, X.-P.; Trapella, C.; Guerrini, R.; Calo, G.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 395–399. doi:10.1038/nature11085
Go to reference 16
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
Go to reference 1
11.Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Mathiesen, J. M.; Sunahara, R. K.; Pardo, L.; Weis, W. I.; Kobilka, B. K.; Granier, S. Nature 2012, 485, 321–326. doi:10.1038/nature10954
Go to reference 11
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
8.Kane, B. E.; McCurdy, C. R.; Ferguson, D. M. J. Med. Chem. 2008, 51, 1824–1830. doi:10.1021/jm701040v
9.Vardy, E.; Mosier, P. D.; Frankowski, K. J.; Wu, H.; Katritch, V.; Westkaemper, R. B.; Aubé, J.; Stevens, R. C.; Roth, B. L. J. Biol. Chem. 2013, 288, 34470–34483. doi:10.1074/jbc.M113.515668
Go to references 7-9
18.Kane, B. E. Molecular recognition of G-protein coupled receptor ligands: Insights into salvinorin A and xanomeline. Ph.D. Thesis, University of Minnesota, 2006.
http://gradworks.umi.com/32/38/3238362.html
Go to reference 18
13.Morphy, R. J. Historical Strategies for Lead Generation. In Designing Multi-Target Drugs; Morphy, R. J.; Harris, C. J., Eds.; RSC: Cambridge, 2012; pp 111–129. doi:10.1039/9781849734912-00111
Go to reference 13
8.Kane, B. E.; McCurdy, C. R.; Ferguson, D. M. J. Med. Chem. 2008, 51, 1824–1830. doi:10.1021/jm701040v
18.Kane, B. E. Molecular recognition of G-protein coupled receptor ligands: Insights into salvinorin A and xanomeline. Ph.D. Thesis, University of Minnesota, 2006.
http://gradworks.umi.com/32/38/3238362.html
Go to references 8,18
19.Zimmerman, D. M.; Leander, J. D.; Cantrell, B. E.; Reel, J. K.; Snoddy, J.; Mendelsohn, L. G.; Johnson, B. G.; Mitch, C. H. J. Med. Chem. 1993, 36, 2833–2841. doi:10.1021/jm00072a001
20.Merz, H.; Langbein, A.; Stockhaus, K.; Walther, G.; Wick, H. Adv. Biochem. Psychopharmacol. 1974, 8, 91–107.
Go to references 19,20
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
Go to reference 1
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
Go to reference 7
19.Zimmerman, D. M.; Leander, J. D.; Cantrell, B. E.; Reel, J. K.; Snoddy, J.; Mendelsohn, L. G.; Johnson, B. G.; Mitch, C. H. J. Med. Chem. 1993, 36, 2833–2841. doi:10.1021/jm00072a001
Go to reference 19
10.Granier, S.; Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Weis, W. I.; Kobilka, B. K. Nature 2012, 485, 400–404. doi:10.1038/nature11111
11.Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Mathiesen, J. M.; Sunahara, R. K.; Pardo, L.; Weis, W. I.; Kobilka, B. K.; Granier, S. Nature 2012, 485, 321–326. doi:10.1038/nature10954
Go to references 10,11
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
Go to reference 1
12.Valant, C.; Lane, J. R.; Sexton, P. M.; Christopoulos, A. Annu. Rev. Pharmacol. Toxicol. 2012, 52, 153–178. doi:10.1146/annurev-pharmtox-010611-134514
Go to reference 12
5.Béguin, C.; Duncan, K. K.; Munro, T. A.; Ho, D. M.; Xu, W.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. 2009, 17, 1370–1380. doi:10.1016/j.bmc.2008.12.012
Go to reference 5
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
8.Kane, B. E.; McCurdy, C. R.; Ferguson, D. M. J. Med. Chem. 2008, 51, 1824–1830. doi:10.1021/jm701040v
9.Vardy, E.; Mosier, P. D.; Frankowski, K. J.; Wu, H.; Katritch, V.; Westkaemper, R. B.; Aubé, J.; Stevens, R. C.; Roth, B. L. J. Biol. Chem. 2013, 288, 34470–34483. doi:10.1074/jbc.M113.515668
Go to references 7-9
33.Rothman, R. B.; Murphy, D. L.; Xu, H.; Godin, J. A.; Dersch, C. M.; Partilla, J. S.; Tidgewell, K.; Schmidt, M.; Prisinzano, T. E. J. Pharmacol. Exp. Ther. 2007, 320, 801–810. doi:10.1124/jpet.106.113167
Go to reference 33
17.Venkatakrishnan, A. J.; Deupi, X.; Lebon, G.; Tate, C. G.; Schertler, G. F.; Babu, M. M. Nature 2013, 494, 185–194. doi:10.1038/nature11896
Go to reference 17
12.Valant, C.; Lane, J. R.; Sexton, P. M.; Christopoulos, A. Annu. Rev. Pharmacol. Toxicol. 2012, 52, 153–178. doi:10.1146/annurev-pharmtox-010611-134514
Go to reference 12
13.Morphy, R. J. Historical Strategies for Lead Generation. In Designing Multi-Target Drugs; Morphy, R. J.; Harris, C. J., Eds.; RSC: Cambridge, 2012; pp 111–129. doi:10.1039/9781849734912-00111
Go to reference 13
12.Valant, C.; Lane, J. R.; Sexton, P. M.; Christopoulos, A. Annu. Rev. Pharmacol. Toxicol. 2012, 52, 153–178. doi:10.1146/annurev-pharmtox-010611-134514
Go to reference 12
36.Béguin, C.; Richards, M. R.; Li, J.-G.; Wang, Y.; Xu, W.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. Lett. 2006, 16, 4679–4685. doi:10.1016/j.bmcl.2006.05.093
Go to reference 36
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
Go to reference 7
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
Go to reference 1
10.Granier, S.; Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Weis, W. I.; Kobilka, B. K. Nature 2012, 485, 400–404. doi:10.1038/nature11111
Go to reference 10
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
Go to reference 1
10.Granier, S.; Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Weis, W. I.; Kobilka, B. K. Nature 2012, 485, 400–404. doi:10.1038/nature11111
Go to reference 10
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
Go to reference 7
11.Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Mathiesen, J. M.; Sunahara, R. K.; Pardo, L.; Weis, W. I.; Kobilka, B. K.; Granier, S. Nature 2012, 485, 321–326. doi:10.1038/nature10954
Go to reference 11
15.Melief, E. J.; Miyatake, M.; Carroll, F. I.; Béguin, C.; Carlezon, W. A., Jr.; Cohen, B. M.; Grimwood, S.; Mitch, C. H.; Rorick-Kehn, L.; Chavkin, C. Mol. Pharmacol. 2011, 80, 920–929. doi:10.1124/mol.111.074195
Go to reference 15
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
Go to reference 7
21.Hiller, C.; Kühhorn, J.; Gmeiner, P. J. Med. Chem. 2013, 56, 6542–6559. doi:10.1021/jm4004335
Go to reference 21
14.Béguin, C.; Cohen, B. M. Medicinal Chemistry of Kappa Opioid Receptor Antagonists. In Opiate Receptors and Antagonists: from Bench to Clinic; Dean, R. L.; Bilsky, E. J.; Negus, S. S., Eds.; Humana Press: New York, 2009; pp 99–118. doi:10.1007/978-1-59745-197-0_6
Go to reference 14
13.Morphy, R. J. Historical Strategies for Lead Generation. In Designing Multi-Target Drugs; Morphy, R. J.; Harris, C. J., Eds.; RSC: Cambridge, 2012; pp 111–129. doi:10.1039/9781849734912-00111
Go to reference 13
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
Go to reference 7
31.Munro, T. A. The Chemistry of Salvia divinorum. Ph.D. Thesis, University of Melbourne, Australia, 2006.
http://pqdtopen.proquest.com/#abstract?dispub=3304556
Go to reference 31
34.May, L. T.; Leach, K.; Sexton, P. M.; Christopoulos, A. Annu. Rev. Pharmacol. Toxicol. 2007, 47, 1–51. doi:10.1146/annurev.pharmtox.47.120505.105159
Go to reference 34
5.Béguin, C.; Duncan, K. K.; Munro, T. A.; Ho, D. M.; Xu, W.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. 2009, 17, 1370–1380. doi:10.1016/j.bmc.2008.12.012
Go to reference 5
36.Béguin, C.; Richards, M. R.; Li, J.-G.; Wang, Y.; Xu, W.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. Lett. 2006, 16, 4679–4685. doi:10.1016/j.bmcl.2006.05.093
Go to reference 36
24.Munro, T. A.; Duncan, K. K.; Xu, W.; Wang, Y.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M.; Béguin, C. Bioorg. Med. Chem. 2008, 16, 1279–1286. doi:10.1016/j.bmc.2007.10.067
Go to reference 24
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
8.Kane, B. E.; McCurdy, C. R.; Ferguson, D. M. J. Med. Chem. 2008, 51, 1824–1830. doi:10.1021/jm701040v
9.Vardy, E.; Mosier, P. D.; Frankowski, K. J.; Wu, H.; Katritch, V.; Westkaemper, R. B.; Aubé, J.; Stevens, R. C.; Roth, B. L. J. Biol. Chem. 2013, 288, 34470–34483. doi:10.1074/jbc.M113.515668
Go to references 7-9
23.Wang, Y.; Chen, Y.; Xu, W.; Lee, D. Y. W.; Ma, Z.; Rawls, S. M.; Cowan, A.; Liu-Chen, L. Y. J. Pharmacol. Exp. Ther. 2008, 324, 1073–1083. doi:10.1124/jpet.107.132142
24.Munro, T. A.; Duncan, K. K.; Xu, W.; Wang, Y.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M.; Béguin, C. Bioorg. Med. Chem. 2008, 16, 1279–1286. doi:10.1016/j.bmc.2007.10.067
Go to references 23,24
10.Granier, S.; Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Weis, W. I.; Kobilka, B. K. Nature 2012, 485, 400–404. doi:10.1038/nature11111
Go to reference 10
6.Béguin, C.; Potuzak, J.; Xu, W.; Liu-Chen, L.-Y.; Streicher, J. M.; Groer, C. E.; Bohn, L. M.; Carlezon, W. A., Jr.; Cohen, B. M. Bioorg. Med. Chem. Lett. 2012, 22, 1023–1026. doi:10.1016/j.bmcl.2011.11.128
Go to reference 6
11.Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Mathiesen, J. M.; Sunahara, R. K.; Pardo, L.; Weis, W. I.; Kobilka, B. K.; Granier, S. Nature 2012, 485, 321–326. doi:10.1038/nature10954
Go to reference 11
32.Neubig, R. R.; Spedding, M.; Kenakin, T.; Christopoulos, A. Pharmacol. Rev. 2003, 55, 597–606. doi:10.1124/pr.55.4.4
Go to reference 32
10.Granier, S.; Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Weis, W. I.; Kobilka, B. K. Nature 2012, 485, 400–404. doi:10.1038/nature11111
Go to reference 10
33.Rothman, R. B.; Murphy, D. L.; Xu, H.; Godin, J. A.; Dersch, C. M.; Partilla, J. S.; Tidgewell, K.; Schmidt, M.; Prisinzano, T. E. J. Pharmacol. Exp. Ther. 2007, 320, 801–810. doi:10.1124/jpet.106.113167
Go to reference 33
34.May, L. T.; Leach, K.; Sexton, P. M.; Christopoulos, A. Annu. Rev. Pharmacol. Toxicol. 2007, 47, 1–51. doi:10.1146/annurev.pharmtox.47.120505.105159
Go to reference 34
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
8.Kane, B. E.; McCurdy, C. R.; Ferguson, D. M. J. Med. Chem. 2008, 51, 1824–1830. doi:10.1021/jm701040v
9.Vardy, E.; Mosier, P. D.; Frankowski, K. J.; Wu, H.; Katritch, V.; Westkaemper, R. B.; Aubé, J.; Stevens, R. C.; Roth, B. L. J. Biol. Chem. 2013, 288, 34470–34483. doi:10.1074/jbc.M113.515668
Go to references 7-9
35.Knoll, A. T.; Carlezon, W. A., Jr. Brain Res. 2010, 1314, 56–73. doi:10.1016/j.brainres.2009.09.074
Go to reference 35
1.Cunningham, C. W.; Rothman, R. B.; Prisinzano, T. E. Pharmacol. Rev. 2011, 63, 316–347. doi:10.1124/pr.110.003244
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
8.Kane, B. E.; McCurdy, C. R.; Ferguson, D. M. J. Med. Chem. 2008, 51, 1824–1830. doi:10.1021/jm701040v
9.Vardy, E.; Mosier, P. D.; Frankowski, K. J.; Wu, H.; Katritch, V.; Westkaemper, R. B.; Aubé, J.; Stevens, R. C.; Roth, B. L. J. Biol. Chem. 2013, 288, 34470–34483. doi:10.1074/jbc.M113.515668
Go to references 1,7-9
7.Wu, H.; Wacker, D.; Mileni, M.; Katritch, V.; Han, G. W.; Vardy, E.; Liu, W.; Thompson, A. A.; Huang, X.-P.; Carroll, F. I.; Mascarella, S. W.; Westkaemper, R. B.; Mosier, P. D.; Roth, B. L.; Cherezov, V.; Stevens, R. C. Nature 2012, 485, 327–332. doi:10.1038/nature10939
Go to reference 7
29.Harding, W. W.; Schmidt, M.; Tidgewell, K.; Kannan, P.; Holden, K. G.; Dersch, C. M.; Rothman, R. B.; Prisinzano, T. E. Bioorg. Med. Chem. Lett. 2006, 16, 3170–3174. doi:10.1016/j.bmcl.2006.03.062
Go to reference 29
24.Munro, T. A.; Duncan, K. K.; Xu, W.; Wang, Y.; Liu-Chen, L.-Y.; Carlezon, W. A., Jr.; Cohen, B. M.; Béguin, C. Bioorg. Med. Chem. 2008, 16, 1279–1286. doi:10.1016/j.bmc.2007.10.067
Go to reference 24
30.Ortega, A.; Blount, J. F.; Manchand, P. S. J. Chem. Soc., Perkin Trans. 1 1982, 2505–2508. doi:10.1039/P19820002505
31.Munro, T. A. The Chemistry of Salvia divinorum. Ph.D. Thesis, University of Melbourne, Australia, 2006.
http://pqdtopen.proquest.com/#abstract?dispub=3304556
Go to references 30,31
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