Antibacterial activity of a berberine nanoformulation

Hue Thi Nguyen, Tuyet Nhung Pham, Anh-Tuan Le, Nguyen Thanh Thuy, Tran Quang Huy and Thuy Thi Thu Nguyen

Beilstein J. Nanotechnol. 2022, 13, 641–652. https://doi.org/10.3762/bjnano.13.56

Supporting Information

Supporting Information features the raw DLS measurement data.

Supporting Information File 1: Raw DLS data.
Format: PDF	Size: 504.0 KB	Download

Cite the Following Article

Antibacterial activity of a berberine nanoformulation

Hue Thi Nguyen, Tuyet Nhung Pham, Anh-Tuan Le, Nguyen Thanh Thuy, Tran Quang Huy and Thuy Thi Thu Nguyen

Beilstein J. Nanotechnol. 2022, 13, 641–652. https://doi.org/10.3762/bjnano.13.56

How to Cite

Nguyen, H. T.; Pham, T. N.; Le, A.-T.; Thuy, N. T.; Huy, T. Q.; Nguyen, T. T. T. Beilstein J. Nanotechnol. 2022, 13, 641–652. doi:10.3762/bjnano.13.56

Download Citation

Citation data can be downloaded as file using the "Download" button or used for copy/paste from the text window below.
Citation data in RIS format can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Zotero.

File format:

RIS

BibTeX

Include:

Citation for the article above

Citation and complete reference list for the article above

Download

TY  - JOUR
A1  - Nguyen, Hue Thi
A1  - Pham, Tuyet Nhung
A1  - Le, Anh-Tuan
A1  - Thuy, Nguyen Thanh
A1  - Huy, Tran Quang
A1  - Nguyen, Thuy Thi Thu
T1  - Antibacterial activity of a berberine nanoformulation
JF  - Beilstein Journal of Nanotechnology
PY  - 2022///
VL  - 13
SP  - 641
EP  - 652
SN  - 2190-4286
DO  - 10.3762/bjnano.13.56
PB  - Beilstein-Institut
JA  - Beilstein J. Nanotechnol.
UR  - https://doi.org/10.3762/bjnano.13.56
KW  - antibacterial activity
KW  - antisolvent precipitation (ASP)
KW  - berberine nanoparticles (BBR NPs)
KW  - glycerol
KW  - solubility
N2  - This study describes the preparation of berberine (BBR) in nanoformulation to enhance its solubility and increase its antibacterial effectiveness against hospital-acquired infections. BBR nanoparticles (BBR NPs) were formed by antisolvent precipitation (ASP) using glycerol as a safe organic solvent. UV–vis absorption spectra demonstrated that the solubility of BBR NPs was greatly enhanced compared to that of pure BBR. Glycerol played a role as a stabilizer for BBR NPs through the formation of hydrogen bonds between glycerol and BBR NPs. The prepared BBR NPs have a narrow size distribution with an average diameter of 156 nm at a concentration of 2.0 mg/mL, measured by dynamic light scattering. After nanoformulation, the concentration of BBR NPs could reach up to 5.0 mg/mL, which is much higher than the saturation concentration without treatment. Results show a strongly enhanced antibacterial activity of BBR NPs compared with that of pure BBR at the same concentration. The minimum bactericidal concentration of BBR NPs against methicillin-resistant Staphylococcus aureus and Escherichia coli O157:H7 was found to be 2.0 and 5.0 mg/mL, respectively. Transmission electron microscopy showed that BBR NPs surrounded the bacterial cells and severely damaged the cell walls. Therefore, BBR NPs prepared by ASP appear to be a potential candidate for the treatment of bacterial pathogens.
ER  -

Presentation Graphic

Picture with graphical abstract, title and authors for social media postings and presentations.
Format: PNG	Size: 11.6 MB	Download

Citations to This Article

Up to 20 of the most recent references are displayed here.

Scholarly Works

Jiao, M.; Kong, W.; Liu, W.; Dong, Z.; Yang, J.; Wei, Z.; Lu, X.; Wei, Y.; Zhuang, J. Boosting the antibacterial potency of natural products through nanotechnologies. International journal of pharmaceutics 2025, 674, 125437. doi:10.1016/j.ijpharm.2025.125437
Nguyen, T. H.; Pham, H. T.; Nguyen, K. K. T.; Ngo, L. H.; Mai, A. N. T.; Lam, T. H. A.; Phan, N. T. K.; Pham, D. T.; Hoang, D. T.; Nguyen, T. D.; Truong, L. T. X. Fabrication and evaluation of BerNPs regarding the growth and development of Streptococcus mutans. Beilstein journal of nanotechnology 2025, 16, 308–315. doi:10.3762/bjnano.16.23
Pacyga, K.; Pacyga, P.; Szuba, E.; Viscardi, S.; Topola, E.; Duda-Madej, A. Nanotechnology Meets Phytotherapy: A Cutting-Edge Approach to Treat Bacterial Infections. International journal of molecular sciences 2025, 26, 1254. doi:10.3390/ijms26031254
Zhou, F.; Gu, X.; Wang, W.; Lin, M.; Wang, L. Advancements in MRSA treatment: the role of berberine in enhancing antibiotic therapy. BMC microbiology 2024, 24, 540. doi:10.1186/s12866-024-03692-9
Yi, L.; Bai, Y.; Chen, X.; Wang, W.; Zhang, C.; Shang, Z.; Zhang, Z.; Li, J.; Cao, M.; Zhu, Z.; Zhang, J. Synergistic Effects and Mechanisms of Action of Rutin with Conventional Antibiotics Against Escherichia coli. International journal of molecular sciences 2024, 25, 13684. doi:10.3390/ijms252413684
Wang, X.; Wei, Z.; Wu, Z.; Li, Y.; Miao, C.; Cao, Z. Thermosensitive Injectable Dual Drug-Loaded Chitosan-Based Hydrogels for Treating Bacterial Endometritis. ACS biomaterials science & engineering 2024, 10, 7516–7526. doi:10.1021/acsbiomaterials.4c01729
Li, Z.; Lan, J.; Liu, L.; Wang, Y.; Chen, L.; Zeng, R.; Gu, D.; Hu, R.; Zhang, T.; Ding, Y. Versatile Thermo-Sensitive liposomes with HSP inhibition and Anti-Inflammation for synergistic Chemo-Photothermal to inhibit breast cancer metastasis. International journal of pharmaceutics 2024, 664, 124583. doi:10.1016/j.ijpharm.2024.124583
Mary, S. A. A.; Stella, S. Bimetallic magnetic nanoparticles dispersed in glycerol: An efficient hydrophilic catalyst for the reduction of 4‐nitrophenol. Applied Organometallic Chemistry 2024, 38. doi:10.1002/aoc.7604
Mehra, M.; Sheorain, J.; Bakshi, J.; Thakur, R.; Grewal, S.; Dhingra, D.; Kumari, S. Synthesis and evaluation of berberine loaded chitosan nanocarrier for enhanced in-vitro antioxidant and anti-inflammatory potential. Carbohydrate Polymer Technologies and Applications 2024, 7, 100474. doi:10.1016/j.carpta.2024.100474
Le, L. T.; Nguyen, H. T.; Nguyen, L. T.; Tran, H. Q.; Nguyen, T. T. T. Berberine-loaded polylactic acid nanofiber scaffold as a drug delivery system: The relationship between chemical characteristics, drug-release behavior, and antibacterial efficiency. Beilstein journal of nanotechnology 2024, 15, 71–82. doi:10.3762/bjnano.15.7
Nguyen, H. T.; Pham, T. N.; Le, L. T.; Nguyen, T. K.; Le, A.-T.; Huy, T. Q.; Thu Nguyen, T. T. Complexes of Ag and ZnO nanoparticles with BBR for enhancement of gastrointestinal antibacterial activity through the impacts of size and composition. RSC advances 2023, 13, 6027–6037. doi:10.1039/d3ra00053b
AbouAitah, K.; Ahn, G.; Ahn, J.-Y.; Kim, B. S. A nano-delivery system combining natural agents and phototherapy application enhances the combating of drug-resistant bacteria. Chemical Engineering Journal 514, 163305. doi:10.1016/j.cej.2025.163305

Explore citations to this article at

Back To Article

Other Beilstein-Institut Open Science Activities

aromatic	the word “aromatic”
aromatic aldehyde	the word “aromatic” OR “aldehyde”
+aromatic +aldehyde	both words “aromatic” AND “aldehyde”
+aromatic -aldehyde	the word “aromatic” but NOT “aldehyde”
“aromatic aldehyde”	the exact phrase “aromatic aldehyde”
benz*	words which begin with “benz”, such as “benzene” or “benzyl”
benz*yl	words that begin with “benz” and end with “yl”, such as “benzyl” or “benzoyl”
benzyl~	words that are close to the word “benzyl”, such as “benzoyl” (i.e., fuzzy search)