DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

Nathalie B. F. Almeida, Thiago A. S. L. Sousa, Viviane C. F. Santos, Camila M. S. Lacerda, Thais G. Silva, Rafaella F. Q. Grenfell, Flavio Plentz and Antero S. R. Andrade

Beilstein J. Nanotechnol. 2022, 13, 873–881. https://doi.org/10.3762/bjnano.13.78

Supporting Information

Optical microscopy image of our typical CVD graphene device, schematic illustration of the resulting ZIKV60-functionalized graphene devices and experimental setup used in the electrical characterization for ZIKV NS1 protein detection, flowchart outlining the execution of CE-SELEX for ZIKV NS1 protein aptamers selection, secondary structure of ZIKV60 aptamer, transfer curves of four distinct graphene devices prior and subsequent to functionalization with ZIKV60 aptamer, transfer curves of six individual graphene devices functionalized with ZIKV60 aptamer before and after sequential addition of ZIKV NS1 protein ranging from 0.01 to 1000 pg/mL, graphene resistances at VG = 0.2 V of seven different devices for the following ZIKV NS1 protein concentrations: 0.01, 0.1, 1, 10, 100, and 1000 pg/mL, percentage changes in graphene resistance at VG = 0.2 V for the following ZIKV NS1 protein concentrations: 0.01, 0.1, 1, 10, 100, and 1000 pg/mL.

Supporting Information File 1: Additional experimental data.
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