DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

Nathalie B. F. Almeida, Thiago A. S. L. Sousa, Viviane C. F. Santos, Camila M. S. Lacerda, Thais G. Silva, Rafaella F. Q. Grenfell, Flavio Plentz and Antero S. R. Andrade

Beilstein J. Nanotechnol. 2022, 13, 873–881. https://doi.org/10.3762/bjnano.13.78

Supporting Information

Optical microscopy image of our typical CVD graphene device, schematic illustration of the resulting ZIKV60-functionalized graphene devices and experimental setup used in the electrical characterization for ZIKV NS1 protein detection, flowchart outlining the execution of CE-SELEX for ZIKV NS1 protein aptamers selection, secondary structure of ZIKV60 aptamer, transfer curves of four distinct graphene devices prior and subsequent to functionalization with ZIKV60 aptamer, transfer curves of six individual graphene devices functionalized with ZIKV60 aptamer before and after sequential addition of ZIKV NS1 protein ranging from 0.01 to 1000 pg/mL, graphene resistances at VG = 0.2 V of seven different devices for the following ZIKV NS1 protein concentrations: 0.01, 0.1, 1, 10, 100, and 1000 pg/mL, percentage changes in graphene resistance at VG = 0.2 V for the following ZIKV NS1 protein concentrations: 0.01, 0.1, 1, 10, 100, and 1000 pg/mL.

Supporting Information File 1: Additional experimental data.
Format: PDF Size: 1.1 MB Download

Presentation Graphic

Picture with graphical abstract, title and authors for social media postings and presentations.

Format: PNG Size: 11.8 MB Download
Back To Article

Latest Articles

Recent advances in green carbon dots (2015–2022): synthesis, metal ion sensing, and biological applications

  1. Aisha Kanwal,
  2. Naheed Bibi,
  3. Sajjad Hyder,
  4. Arif Muhammad,
  5. Hao Ren,
  6. Jiangtao Liu and
  7. Zhongli Lei
  • Review
  • Published 05 Oct 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 1068–1107, doi:10.3762/bjnano.13.93

Biomimetic chitosan with biocomposite nanomaterials for bone tissue repair and regeneration

  1. Se-Kwon Kim,
  2. Sesha Subramanian Murugan,
  3. Pandurang Appana Dalavi,
  4. Sebanti Gupta,
  5. Sukumaran Anil,
  6. Gi Hun Seong and
  7. Jayachandran Venkatesan
  • Review
  • Published 29 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 1051–1067, doi:10.3762/bjnano.13.92

  • Full Research Paper
  • Published 28 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 1038–1050, doi:10.3762/bjnano.13.91

  • Full Research Paper
  • Published 27 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 1030–1037, doi:10.3762/bjnano.13.90

Electrocatalytic oxygen reduction activity of AgCoCu oxides on reduced graphene oxide in alkaline media

  1. Iyyappan Madakannu,
  2. Indrajit Patil,
  3. Bhalchandra Kakade and
  4. Kasibhatta Kumara Ramanatha Datta
  • Full Research Paper
  • Published 26 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 1020–1029, doi:10.3762/bjnano.13.89

  • Full Research Paper
  • Published 23 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 1011–1019, doi:10.3762/bjnano.13.88

Effects of focused electron beam irradiation parameters on direct nanostructure formation on Ag surfaces

  1. Jānis Sniķeris,
  2. Vjačeslavs Gerbreders,
  3. Andrejs Bulanovs and
  4. Ēriks Sļedevskis
  • Full Research Paper
  • Published 22 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 1004–1010, doi:10.3762/bjnano.13.87

  • Full Research Paper
  • Published 21 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 986–1003, doi:10.3762/bjnano.13.86

Theoretical investigations of oxygen vacancy effects in nickel-doped zirconia from ab initio XANES spectroscopy at the oxygen K-edge

  1. Dick Hartmann Douma,
  2. Lodvert Tchibota Poaty,
  3. Alessio Lamperti,
  4. Stéphane Kenmoe,
  5. Abdulrafiu Tunde Raji,
  6. Alberto Debernardi and
  7. Bernard M’Passi-Mabiala
  • Full Research Paper
  • Published 15 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 975–985, doi:10.3762/bjnano.13.85

Interaction between honeybee mandibles and propolis

  1. Leonie Saccardi,
  2. Franz Brümmer,
  3. Jonas Schiebl,
  4. Oliver Schwarz,
  5. Alexander Kovalev and
  6. Stanislav Gorb
  • Full Research Paper
  • Published 14 Sep 2022
Graphical Abstract

Beilstein J. Nanotechnol. 2022, 13, 958–974, doi:10.3762/bjnano.13.84

Latest Thematic Issues

Other Beilstein-Institut Open Science Activities

Keep Informed

RSS Feed

Subscribe to our Latest Articles RSS Feed.

Subscribe

Follow the Beilstein-Institut

LinkedIn

Twitter: @BeilsteinInst