Influence of surface chemical properties on the toxicity of engineered zinc oxide nanoparticles to embryonic zebrafish

Zitao Zhou, Jino Son, Bryan Harper, Zheng Zhou and Stacey Harper

Beilstein J. Nanotechnol. 2015, 6, 1568–1579. doi:10.3762/bjnano.6.160

Supporting Information

Supporting Information File 1: Zebrafish malformation and behavioral data. The 19 sub-lethal endpoints are developmental progression (DP), spontaneous movement (SP), notochord (N), yolk sac edema (Y), axis (A), eye (E), snout (Sn), jaw (J), otic (O), heart (H), brain (B), somite (So), pectoral fin (PF), caudal fin (CF), pigment (P), circulation (C), trunk (T), swim bladder (SB), and touch response (TR).
Format: XLSX Size: 20.0 KB Download
Supporting Information File 2: Fisher’s exact test p-value. The 19 sub-lethal endpoints are developmental progression (DP), spontaneous movement (SP), notochord (N), yolk sac edema (Y), axis (A), eye (E), snout (Sn), jaw (J), otic (O), heart (H), brain (B), somite (So), pectoral fin (PF), caudal fin (CF), pigment (P), circulation (C), trunk (T), swim bladder (SB), and touch response (TR). Included are three mortality (M) endpoints at 24 and 120 hours post fertilization after the exposure to ZnO NP and the sum of two M.
Format: XLSX Size: 64.0 KB Download
Supporting Information File 3: Cluster analysis of converted data using Euclidean distance to partition into A) 3, B) 4, C) 5, D) 6 clusters.
Format: PNG Size: 249.3 KB Download
Supporting Information File 4: Kriging estimations of zebrafish mortality data at A) 0.016 ppm, B) 0.08 ppm, C) 0.4 ppm, D) 2 ppm, E) 10 ppm, F) 50 ppm.
Format: PNG Size: 829.5 KB Download
Supporting Information File 5: Embryonic zebrafish mortality at 24 and 120 hours post fertilization after ZnO NP exposure.
Format: XLSX Size: 56.7 KB Download
Supporting Information File 6: XRD analysis of three different ZnO NPs.
Format: PNG Size: 101.1 KB Download
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