Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• least 24 h, subsequently washed with potassium phosphate buffer and post-fixed with 1% osmium tetroxide in sodium cacodylate buffer, washed with maleate buffer, and stained en bloc with 0.5% uranyl acetate in maleate buffer. Afterwards, the cells were dehydrated in ascending ethanol series, and embedded