Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• /HTRA1 and 2.7·10−23 m3 for CaP/PEI/HTRA1; m(NP) = 7.5·10−19 kg for CaP/CMC/HTRA1 and 8.3·10−20 kg for CaP/PEI/HTRA1. The concentration of molecules on the nanoparticles was calculated by subtraction of the protein concentration in the supernatant by quantitative UV–vis spectroscopy using a calibration

• dispersion in biological media containing proteins (like RPMI/10% FCS) is much better due to the formation of a protein corona [48][49]. The concentration of the labelled proteins was determined by UV–vis spectroscopy (Table 2). Table 3 gives the quantification data of the functionalized nanoparticles, where

• inhibitors. Mock: Untreated cells. Endocytosis inhibitors used in the cell culture experiments. Characterization of protein-loaded nanoparticles by dynamic light scattering (DLS) and UV–vis spectroscopy (all data recorded in pure water). The particles functionalized with carboxymethylcellulose (CMC) were

Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies

• Xuefeng Wang,
• Zhong Mei,
• Yanyan Wang and
• Liang Tang

Beilstein J. Nanotechnol. 2017, 8, 372–380, doi:10.3762/bjnano.8.39

• at room temperature with stirring, which was then kept for 2 h. The excess reagents were removed by centrifugation and characterized by UV–vis spectroscopy and TEM. Measurement of conjugated anti-IgG by ELISA Standard ELISA was employed with human IgG as the antigen source to quantify the

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

• Maryam Daneshpour,
• Kobra Omidfar and
• Hossein Ghanbarian

Beilstein J. Nanotechnol. 2016, 7, 2023–2036, doi:10.3762/bjnano.7.193

• (Zetasizer nano-ZS, Malvern, England). The NPs were also analyzed using UV–vis spectroscopy (Lambda 950 UV–vis spectrophotometer, Perkin Elmer, USA) at about 520 nm after each preparation step. The magnetic behavior of gold–magnetic NPs was investigated via vibrating sample magnetometer (VSM) (MAG-3110

Functionalized platinum nanoparticles with surface charge trigged by pH: synthesis, characterization and stability studies

• Giovanna Testa,
• Laura Fontana,
• Iole Venditti and
• Ilaria Fratoddi

Beilstein J. Nanotechnol. 2016, 7, 1822–1828, doi:10.3762/bjnano.7.175

• (see Figure 1), suggesting that the band structure of the Pt nanoparticles is formed [34]. After careful purification by centrifugation, the colloidal product was characterized by DLS, UV–vis spectroscopy, FTIR, and ζ-potential measurements. A FESEM study was carried out on selected samples. In Figure

• bond on the PtNP surface. The DLS characterization demonstrated the long-term stability of the colloidal system up to three months and the possibility to tune the aggregation phenomenon of the PtNPs by varying the pH due to modulation of the metal surface charge. Experimental Instrumentation UV–vis

• spectroscopy was performed in H2O solutions using quartz cells with a Varian Cary 100 Scan UV–vis spectrophotometer. The diameter and the diameter distribution of MNPs in H2O solution were investigated at 25 ± 0.2 °C (pH 7 and pH 2) by means of dynamic light scattering (DLS) technique by using a Malvern Nano

Effective intercalation of zein into Na-montmorillonite: role of the protein components and use of the developed biointerfaces

• Ana C. S. Alcântara,
• Margarita Darder,
• Pilar Aranda and
• Eduardo Ruiz-Hitzky

Beilstein J. Nanotechnol. 2016, 7, 1772–1782, doi:10.3762/bjnano.7.170

• biohybrids based on EXT show a lower amount of adsorbed protein than those prepared from PCT. This fact may be related to the adsorption of zein oligomers present in the PCT fraction, as shown by SDS-PAGE (Figure 1B). Adsorption of the components from EXT on MMT is confirmed from analysis by UV–vis

• spectroscopy of the supernatant separated after formation of the EXT–MMT biohybrids (Supporting Information File 1, Figure S6), which shows clearly the decrease of intensity in the bands ascribed to protein and carotenoid components with respect to those in the EXT phase. The X-ray diffractograms of the EXT

Hydrophilic silver nanoparticles with tunable optical properties: application for the detection of heavy metals in water

• Paolo Prosposito,
• Federico Mochi,
• Erica Ciotta,
• Mauro Casalboni,
• Fabio De Matteis,
• Iole Venditti,
• Laura Fontana,
• Giovanna Testa and
• Ilaria Fratoddi

Beilstein J. Nanotechnol. 2016, 7, 1654–1661, doi:10.3762/bjnano.7.157

• one (synthesis a) the procedure was developed in analogy with previous work [22] and is reported in Scheme 1. This step leads to the hydrophilic character of silver nanoparticles. At the end of the synthesis and purification, the reaction product was analyzed by UV–vis spectroscopy to confirm the

• nanoparticles with an average diameter of 2.5 ± 0.3 nm were synthetized with a narrow distribution range and negative charge on the surface. These particles were fully characterized by means of different techniques, such as STM, DLS and UV–vis spectroscopy. The SPR signal of the AgNP-3MPS sample in water

Manufacturing and investigation of physical properties of polyacrylonitrile nanofibre composites with SiO₂, TiO₂ and Bi₂O₃ nanoparticles

• Tomasz Tański,
• Wiktor Matysiak and
• Barbara Hajduk

Beilstein J. Nanotechnol. 2016, 7, 1141–1155, doi:10.3762/bjnano.7.106

• properties of the obtained composite nanofibres with the participation of fibrous layers are scrutinized by means of ellipsometry and UV–vis spectroscopy. Experimental In order to prepare the spinning solution, the following were used: polyacrylonitrile (PAN purity 99%, Mw = 150 000 g/mol). The reinforcing

• fibrous mats, determined using ellipsometry studies and the UV–vis analysis. Each of the used methods showed that the energy gap width increased with increasing mass concentration of the reinforcing phase (Table 4). With the UV–vis spectroscopy measurements, the smallest energy gap width was recorded for

Sandwich-like layer-by-layer assembly of gold nanoparticles with tunable SERS properties

• Zhicheng Liu,
• Lu Bai,
• Guizhe Zhao and
• Yaqing Liu

Beilstein J. Nanotechnol. 2016, 7, 1028–1032, doi:10.3762/bjnano.7.95

• -prepared substrate and left to dry in the air. The as-synthesized Au NP solution was characterized by UV–visible (UV–vis) spectroscopy (Cary 5000). Field emission scanning electron microscopy (FE-SEM, Hitachi S-4800) and transmission electron microscopy (TEM, JEOL JEM 1011) were used to image the LbL thin

Facile synthesis of water-soluble carbon nano-onions under alkaline conditions

• Gaber Hashem Gaber Ahmed,
• Rosana Badía Laíño,
• Josefa Angela García Calzón and
• Marta Elena Díaz García

Beilstein J. Nanotechnol. 2016, 7, 758–766, doi:10.3762/bjnano.7.67

• leaves (acer saccharum), under the same temperature and alkaline conditions did not produce carbon onions. XRD, FTIR, HRTEM, UV–vis spectroscopy, and photoluminescence analyses were performed to characterize the as-synthesized carbon nanomaterials. Preliminary tests demonstrate a capability of the

Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform

• M. Fátima Barroso,
• M. Alejandra Luna,
• Juan S. Flores Tabares,
• Cristina Delerue-Matos,
• N. Mariano Correa,
• Fernando Moyano and
• Patricia G. Molina

Beilstein J. Nanotechnol. 2016, 7, 655–663, doi:10.3762/bjnano.7.58

• order to covalently bind the AuNPs to the bilayer and to immobilize the vesicle on the Au electrode. This nanosystem was characterized by using UV–vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS) and cyclic voltammetry (CV). The results show that the vesicles were

• sensible to the conditions under which they are formed. As the irradiation time modifies the nanoparticle morphology we decided to irradiate for longer time, in this case, 13 min. Some techniques, such as UV–vis spectroscopy and TEM were used to perform the morphological characterization of the AuNPs

• measurements can be statistically significant for all the systems investigated. The algorithm used was CONTIN and the DLS experiments shown that the polydispersities of the LUVs were less than 5%. UV–vis spectroscopy In order to study the formation of AuNPs, UV–vis studies were performed by using Shimadzu 2401

Bacteriorhodopsin–ZnO hybrid as a potential sensing element for low-temperature detection of ethanol vapour

• Saurav Kumar,
• Sudeshna Bagchi,
• Senthil Prasad,
• Anupma Sharma,
• Ritesh Kumar,
• Rishemjit Kaur,
• Jagvir Singh and
• Amol P. Bhondekar

Beilstein J. Nanotechnol. 2016, 7, 501–510, doi:10.3762/bjnano.7.44

• nonhybrid structures, as is evident from Figure 2c,d. The structural and optical properties of ZnO-TF, ZnO-NR and their hybrid structures were assessed using UV–vis spectroscopy, photoluminescence spectroscopy (PL), Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR). Figure 3 shows the

Hemolysin coregulated protein 1 as a molecular gluing unit for the assembly of nanoparticle hybrid structures

• Tuan Anh Pham,
• Andreas Schreiber,
• Elena V. Sturm (née Rosseeva),
• Stefan Schiller and
• Helmut Cölfen

Beilstein J. Nanotechnol. 2016, 7, 351–363, doi:10.3762/bjnano.7.32

• gluing unit for the assembly of often linear, hybrid structures of plasmonic gold (Au NP), magnetite (Fe3O4 NP), and cobalt ferrite nanoparticles (CoFe2O4 NP). Furthermore, the assembly of Au NPs into linear structures using Hcp1_cys3 is investigated by UV–vis spectroscopy, TEM and cryo-TEM. One key

• nanomaterials. Furthermore, kinetic investigation of the formation of such 1D Au NP structures and the utilization of this structure, for example, as a SERS template and catalyst are also of great interest. The formation kinetics of Au NP networks triggered by Hcp1_cys3 is investigated using UV–vis spectroscopy

• chains, resulting in three protein-based NP hybrid structures on the meso- and micro-scale. The Au NP Hcp1_cys3 assemblies show the formation of short chains in the nanometer range which grow to longer branched chains in UV–vis spectroscopy and TEM/cryo-TEM kinetic investigations. Finally, a network

Time-dependent growth of crystalline Au⁰-nanoparticles in cyanobacteria as self-reproducing bioreactors: 2. Anabaena cylindrica

• Liz M. Rösken,
• Felix Cappel,
• Susanne Körsten,
• Christian B. Fischer,
• Andreas Schönleber,
• Sander van Smaalen,
• Stefan Geimer,
• Christian Beresko,
• Georg Ankerhold and
• Stefan Wehner

Beilstein J. Nanotechnol. 2016, 7, 312–327, doi:10.3762/bjnano.7.30

• electron microscope. These images reveal the spatial distribution of the nanoparticles inside the cells as it was in the moment when their biosynthesis was stopped. Laser-induced breakdown spectroscopy In recent years UV–vis spectroscopy has often been used for the in situ observation of nanoparticle or

• nanocluster development [46][47][48] of various metals, e.g., palladium, gold and silver [46]. Although UV–vis spectroscopy based methods have been developed to determine the size of nanoparticles resp. nanoclusters in solution [47][48]. UV–vis spectroscopy was also tested for the type of system used here in

Single-molecule mechanics of protein-labelled DNA handles

• Vivek S. Jadhav,
• Dorothea Brüggemann,
• Florian Wruck and
• Martin Hegner

Beilstein J. Nanotechnol. 2016, 7, 138–148, doi:10.3762/bjnano.7.16

• kb DNA ladder (NEB & Promega). We utilized UV–vis spectroscopy (Nanodrop spectrophotometer ND-1000, Fisher Scientific, Dublin, Ireland) to measure the concentration of the DHs and PDH constructs. These measurements were also indicative for the presence of protein after 90 min of incubation and the

Impact of ultrasonic dispersion on the photocatalytic activity of titania aggregates

• Hoai Nga Le,
• Frank Babick,
• Klaus Kühn,
• Minh Tan Nguyen,
• Michael Stintz and
• Gianaurelio Cuniberti

Beilstein J. Nanotechnol. 2015, 6, 2423–2430, doi:10.3762/bjnano.6.250

• , the transmittance of the 100× diluted samples from the original 1 g/L suspensions were measured. In order to measure the amount of MB remaining in the solution for the discoloration tests, we measured the absorbance in the supernatant suspensions through 10 mm optical path length by UV–vis

• spectroscopy. The MB concentrations were calibrated from the absorbance at λ = 664 nm [19] (the calibration curve can be found in Figure S2 of Supporting Information File 1) and are plotted as a function of irradiation time. Results and Discussion Verification of the kinetic model In essence, an increase of

Green synthesis, characterization and catalytic activity of natural bentonite-supported copper nanoparticles for the solvent-free synthesis of 1-substituted 1H-1,2,3,4-tetrazoles and reduction of 4-nitrophenol

• Akbar Rostami-Vartooni,
• Mohammad Alizadeh and
• Mojtaba Bagherzadeh

Beilstein J. Nanotechnol. 2015, 6, 2300–2309, doi:10.3762/bjnano.6.236

• concentration of 4-NP was monitored at given intervals by using UV–vis spectroscopy and the results are shown in Figure 9. The original absorption peak of 4-NP is centered at about 320 nm and shifts to 400 nm after addition of the NaBH4 solution. This is due to the formation of p-nitrophenolate ions under

Selective porous gates made from colloidal silica nanoparticles

• Roberto Nisticò,
• Paola Avetta,
• Paola Calza,
• Debora Fabbri,
• Giuliana Magnacca and
• Dominique Scalarone

Beilstein J. Nanotechnol. 2015, 6, 2105–2112, doi:10.3762/bjnano.6.215

• range 1–2 nm, probably due to intraparticle voids generated from the elimination of poly(ethylene-oxide) moieties during the calcination step [41]. Transport testing of the functional coating UV–vis spectroscopy was used to study the diffusion of two positively charged chemical probes (i.e., MB and

• Aquamarijn Micro Filtration BV (Zutphen, Netherlands), having on top a Si3N4 membrane (5 mm × 5 mm × 0.6 mm) with macropores arranged in a hexagonal setting and diameter of 5 μm. Supporting Information Supporting Information File 144: External calibration curves performed by UV–vis spectroscopy

A facile method for the preparation of bifunctional Mn:ZnS/ZnS/Fe₃O₄ magnetic and fluorescent nanocrystals

• Houcine Labiadh,
• Tahar Ben Chaabane,
• Romain Sibille,
• Lavinia Balan and
• Raphaël Schneider

Beilstein J. Nanotechnol. 2015, 6, 1743–1751, doi:10.3762/bjnano.6.178

• heterostructures were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), transmission electron microscopy (TEM), UV–vis spectroscopy and photoluminescence (PL) spectroscopy. The PL spectra of Mn:ZnS/ZnS/Fe3O4 quantum dots (QDs) showed marked visible emission around 584 nm related to the 4T1

Synthesis, characterization and in vitro biocompatibility study of Au/TMC/Fe₃O₄ nanocomposites as a promising, nontoxic system for biomedical applications

• Hanieh Shirazi,
• Maryam Daneshpour,
• Soheila Kashanian and
• Kobra Omidfar

Beilstein J. Nanotechnol. 2015, 6, 1677–1689, doi:10.3762/bjnano.6.170

• ) TMC, (c) chitosan/Fe3O4, (d) TMC/Fe3O4, (e) Au/chitosan/Fe3O4, and (f) Au/TMC/Fe3O4. UV–vis spectroscopy of Au nanoparticles as a function of (a) Au nanoparticle concentration and (b) reaction time. TEM images of (a) Au/TMC/Fe3O4 and (c) Au/chitosan/Fe3O4 with respective particle size distributions (b

Synthesis, characterization and in vitro effects of 7 nm alloyed silver–gold nanoparticles

• Simon Ristig,
• Svitlana Chernousova,
• Wolfgang Meyer-Zaika and
• Matthias Epple

Beilstein J. Nanotechnol. 2015, 6, 1212–1220, doi:10.3762/bjnano.6.124

• important factor for subsequent in vitro experiments) was carried out by multiple ultracentrifugation steps. Characterization by transmission electron microscopy (TEM), differential centrifugal sedimentation (DCS), dynamic light scattering (DLS), UV–vis spectroscopy and atomic absorption spectroscopy (AAS

• scattering was monitored at a fixed angle of 173° in backward scattering mode. The primary data were derived from the correlation function of the scattered intensity as a number-weighed size distribution. UV–vis spectroscopy was performed with a Varian Cary 300 instrument. Suprasil® cuvettes with a sample

• /Au nanoparticles and pure Ag and Au nanoparticles. Overview of the absorption maxima in UV–vis spectroscopy of PVP-functionalized Ag/Au nanoparticles as function of the experimentally determined silver molar content (Table 2). The viability of HeLa cells after incubation with alloyed nanoparticles

From lithium to sodium: cell chemistry of room temperature sodium–air and sodium–sulfur batteries

• Philipp Adelhelm,
• Pascal Hartmann,
• Conrad L. Bender,
• Martin Busche,
• Christine Eufinger and
• Juergen Janek

Beilstein J. Nanotechnol. 2015, 6, 1016–1055, doi:10.3762/bjnano.6.105

Morphology control of zinc oxide films via polysaccharide-mediated, low temperature, chemical bath deposition

• Florian Waltz,
• Hans-Christoph Schwarz,
• Andreas M. Schneider,
• Stefanie Eiden and
• Peter Behrens

Beilstein J. Nanotechnol. 2015, 6, 799–808, doi:10.3762/bjnano.6.83

• CBD steps, HYA was added at different time intervals in order to optimize the quality of the resulting films. The properties of the films were studied by means of field emitting scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), UV–vis spectroscopy and electrical conductivity measurements

Tm-doped TiO₂ and Tm₂Ti₂O₇ pyrochlore nanoparticles: enhancing the photocatalytic activity of rutile with a pyrochlore phase

• Desiré M. De los Santos,
• Javier Navas,
• Teresa Aguilar,
• Antonio Sánchez-Coronilla,
• Concha Fernández-Lorenzo,
• Rodrigo Alcántara,
• Jose Carlos Piñero,
• Ginesa Blanco and
• Joaquín Martín-Calleja

Beilstein J. Nanotechnol. 2015, 6, 605–616, doi:10.3762/bjnano.6.62

• spectroscopy and UV–vis spectroscopy. For the samples annealed at 773 and 973 K, anatase phase TiO2 was obtained, predominantly internally doped with Tm3+. ICP–AES showed that a doping concentration of up to 5.8 atom % was obtained without reducing the crystallinity of the samples. The presence of Tm3+ was

• confirmed by X-ray photoelectron spectroscopy and UV–vis spectroscopy: the incorporation of Tm3+ was confirmed by the generation of new absorption bands that could be assigned to Tm3+ transitions. Furthermore, when the samples were annealed at 1173 K, a pyrochlore phase (Tm2Ti2O7) mixed with TiO2 was

• study the optical properties of the samples and to determine the band gap energy, UV–vis spectroscopy in diffuse reflectance (DR–UV–vis) mode was used. The custom-built system was composed of: (i) an integrating sphere (Spectra Tech), (ii) a spectrometer (USB2000+, Ocean Optics), and (iii) a xenon lamp

Novel ZnO:Ag nanocomposites induce significant oxidative stress in human fibroblast malignant melanoma (Ht144) cells

• Syeda Arooj,
• Samina Nazir,
• Akhtar Nadhman,
• Nafees Ahmad,
• Bakhtiar Muhammad,
• Ishaq Ahmad,
• Kehkashan Mazhar and
• Rashda Abbasi

Beilstein J. Nanotechnol. 2015, 6, 570–582, doi:10.3762/bjnano.6.59

• performing assays, we dialyzed our ZnO:Ag nanocomposite suspensions with Spectra Por 6 (mw cutoff 25000) Spectrum® dialysis membrane for 4 h against deionized water. Characterization of nanocomposite The nanocomposites were characterized by SEM, XRD, Rutherford back scattering, UV–vis spectroscopy and DRS

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• was then amine-functionalized through APS treatment for bioconjugation with FITC-IgG by using glutaraldehyde as linker. The characterization of the prepared NPs was carried out through XRD, UV–vis spectroscopy and PL emission spectra, VSM, TEM and SEM. The fluorescence spectra of mercaptosuccinic-acid

• –vis spectroscopy. The decrease in the absorbance band at 400 nm suggested the successful photogeneration of singlet oxygen. The biocompatibility of these nanocomposites was studied by incubating these nanocomposites with human hepatocyte cells (QSG-7701) for 24 h. The results indicated that even after

• 2.5 nm. When the hybrid nanocomposite material was excited at 488 nm, it exhibited only one emission peak at 520 nm corresponding to fluorescein. The effectiveness of these nanocomposites to generate singlet oxygen (1O2) upon light irradiation was studied using 1,3-diphenylisobenzofuran (DPBF) by UV