Beilstein J. Nanotechnol.2011,2, 311–317, doi:10.3762/bjnano.2.36
, either in the stage of synthesis [9], or in a stage of separation of nanotube bundles, e.g., in a prolonged and vigorous ultrasonication (with frequent nanotube cutting as an accompanying process) [10], or (2) via chemical modification of the nanotube surface [11]. A simple chemical treatment of CNTs can
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Figure 1:
SEM micrographs (A–C): A side (A) and a top view (B) of the HACNT film grown on a quartz substrate;...
Beilstein J. Nanotechnol.2010,1, 135–141, doi:10.3762/bjnano.1.16
long-time ultrasonication (30 min) used.
Electrochemical behavior of L-proDH on MWCNTs–modified GC electrode
The electrochemical properties of GC/MWCNTs were first investigated by cyclic voltammetry with K3Fe(CN)6 as the probe. Typical reversible cyclic voltammograms were observed for both the bare and
electrode, a platinum wire as the counter electrode and Ag/AgCl (3 M NaCl) as the reference electrode. The GC electrodes were finely polished by 1.0 μm and 0.05 μm alumina, and cleaned by ultrasonication in Milli-Q water for 30 s before modification.
Enzyme assay
Recombinant dye-linked L-proDH, from
calculated to be 1.8 units mg−1.
Preparation of MWCNTs and L-proDH modified electrodes
A MWCNTs solution was prepared by dispersing 1.0 mg of MWCNTs in 1.0 mL ethanol followed by ultrasonication for 30 min. A 10 μL aliquot of the MWCNTs dispersion solution was dropped onto the top of the pre-treated GC
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Figure 1:
SEM image of MWCNTs-modified GC electrode.