In vitro toxicity and bioimaging studies of gold nanorods formulations coated with biofunctional thiol-PEG molecules and Pluronic block copolymers

• Tianxun Gong,
• Douglas Goh,
• Malini Olivo and
• Ken-Tye Yong

Beilstein J. Nanotechnol. 2014, 5, 546–553, doi:10.3762/bjnano.5.64

• in the overall cell viability. We also demonstrate the use of the functionalized gold nanorods as scattering probes for dark-field imaging of cancer cells thereby demonstrating their biocompatibility. Our results offer a unique solution for the future development of safe scattering color probes for

• , transmission electron microscopy (TEM), cell viability assay, dynamic light scattering (DLS), and dark-field imaging microscopy. The non-specific uptake of these AuNRs by cells was also studied under dark-field microscopy. Our work demonstrates that the coating of AuNRs surfaces with PEG-SH or PEO–PPO–PEO

• molecules significantly improved the colloidal and optical stability of the gold nanoformulation. No aggregation is found even a few weeks after the preparation. More importantly, the cell viability and dark-field imaging studies indicate that the AuNRs functionalized with PEG-SH or PEO–PPO–PEO molecules

Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating

• Tingjun Lei,
• Alicia Fernandez-Fernandez,
• Romila Manchanda,
• Yen-Chih Huang and
• Anthony J. McGoron

Beilstein J. Nanotechnol. 2014, 5, 313–322, doi:10.3762/bjnano.5.35

• themselves. Cytotoxicity assessment Cell viability after five different treatments (laser only, void PGMD NPs w/ laser, IR820-PGMD NPs, IR820-PGMD NPs w/ laser, incubator HT w/ IR820-PGMD NPs) was measured with the Sulforhodamine B colorimetric (SRB) assay 24 h post-treatment, as previously described in our

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

• Nadine Haberl,
• Stephanie Hirn,
• Alexander Wenk,
• Jörg Diendorf,
• Matthias Epple,
• Blair D. Johnston,
• Fritz Krombach,
• Wolfgang G. Kreyling and
• Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

• -dependent decrease in cell viability. In addition, a proinflammatory response was shown by increased levels of tumor necrosis factor-α (TNF-α), macrophage inflammatory protein-2 (MIP-2), and interleukin-1β (IL-1β) [18]. Furthermore, AgNP caused damage to mitochondria and an increased production of reactive

Magnetic-Fe/Fe₃O₄-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages

• Hongwang Wang,
• Tej B. Shrestha,
• Matthew T. Basel,
• Raj K. Dani,
• Gwi-Moon Seo,
• Sivasai Balivada,
• Marla M. Pyle,
• Heidy Prock,
• Olga B. Koper,
• Prem S. Thapa,
• David Moore,
• Ping Li,
• Viktor Chikan,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2012, 3, 444–455, doi:10.3762/bjnano.3.51

• . To assay the cell viability, MTT reagent solution 1:10 (v/v, reagent solution/cell medium) was added to the cells and incubated at 37 °C for 4 h. After incubation, the MTT solution in buffer (1:1, medium/buffer) was added to the medium, incubated overnight, and the absorbance at 550 nm and 690 nm, as

• spectra of MNP-SN38 and free SN38 released from MNP. Toxicity of MNP-SN38 on double stable Mo/Ma after 24 h of loading; the MTT assay was performed for cell viability, and cell viability of 100% is considered in the case of the control group. Double-stable Mo/Ma loaded with MNP-SN38 320 g/mL(medium). a