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Search for "quantification" in Full Text gives 249 result(s) in Beilstein Journal of Nanotechnology. Showing first 200.
Surface-enhanced infrared absorption studies towards a new optical biosensor
Beilstein J. Nanotechnol. 2016, 7, 1736–1742, doi:10.3762/bjnano.7.166
- -enhanced infrared absorption (SEIRA); Introduction Optical biosensors play an important role in the detection and quantification of analytes. Among others, some applications include point-of-care testing (POCT), the monitoring of blood, urine, sudor and respiratory air, and the search for metabolites and
- different measurement techniques in order to gain more information. One idea is to operate a Mach–Zehnder interferometer (MZI) in the mid-infrared, thus combining the interferometric information (quantification) with the characteristic fingerprint (identification) of the attached analyte [6]. An example of
- to the mid-infrared regime, the sensitivity to changes in film thickness is reduced compared to the visible wavelength regime, as the mid-IR wavelength regime is larger by a factor of 12–35 as compared to the visible region. Therefore, IR is less suited for detection and quantification of small
Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
Beilstein J. Nanotechnol. 2016, 7, 1532–1542, doi:10.3762/bjnano.7.147
- animals was only raised by 2.4 °C could be due to the dispersion of heat by the blood flow through those parts of the animal not within the focus of the EA. After hyperthermia, the rats were sacrificed and tumor and liver tissue samples were obtained for quantification of iron content and for pathologic
The effect of dry shear aligning of nanotube thin films on the photovoltaic performance of carbon nanotube–silicon solar cells
Beilstein J. Nanotechnol. 2016, 7, 1486–1491, doi:10.3762/bjnano.7.141
- are many (n,m) species in the distribution, with the M11 region also displaying several components. The data is presented in Figure 2a on the familiar absorbance scale but a simple conversion yields the T550 value commonly used in the quantification of transparent films’ performance. The isotropic
An ellipsometric approach towards the description of inhomogeneous polymer-based Langmuir layers
Beilstein J. Nanotechnol. 2016, 7, 1156–1165, doi:10.3762/bjnano.7.107
- several hundred micrometers. The conversion into thickness maps allows the additional quantification of observed morphologies of certain regions within one map. This helps to investigate and describe inhomogeneous Langmuir layers. Such applications are definitely not limited to polymer-based Langmuir
Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode
Beilstein J. Nanotechnol. 2016, 7, 1104–1112, doi:10.3762/bjnano.7.103
- phosphotungstic blue is relative to the number of aromatic phenolic groups and is used for their quantification, using gallic acid as a standard. An aliquot of 20 μL of the raw juice, 1.58 mL of water and 100 μL of Folin–Ciocalteu reagent was mixed. After waiting 8 min, 300 μL of a solution of sodium carbonate
Advanced atomic force microscopy techniques III
Beilstein J. Nanotechnol. 2016, 7, 1052–1054, doi:10.3762/bjnano.7.98
- and compared with theoretical predictions [12][13], and local charges within single molecules can be measured [14][15], however, the quantification is still under intense discussion. Chemical reactions are triggered and imaged by the AFM tip [16][17] and of course the technique is not limited to
Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84
- was used. The quantification of cell labeling by cytometry was considered superior to Prussian blue image quantification due to the possible adherence of stain both to the cells and to the coated dish surface. By using flow cytometry and measuring the increase of the side scattered light (SSC) of the
Novel roles for well-known players: from tobacco mosaic virus pests to enzymatically active assemblies
Beilstein J. Nanotechnol. 2016, 7, 613–629, doi:10.3762/bjnano.7.54
- enabled a colorimetric detection of catalytic activity and the quantification of glucose [152][166][167]. Specifically, GOx catalyzes glucose oxidation to D-glucono-1,5-lactone, thereby producing hydrogen peroxide [168]. This is a substrate for HRP, which reduces it to water, and, as a side reaction, can
Nanoscale effects in the characterization of viscoelastic materials with atomic force microscopy: coupling of a quasi-three-dimensional standard linear solid model with in-plane surface interactions
Beilstein J. Nanotechnol. 2016, 7, 554–571, doi:10.3762/bjnano.7.49
- . Regarding the quantification of elastic sample behaviors within periodic dynamic measurements, it is important to point out the two frequency limits for which the response of the 1D SLS model is purely elastic (see Figure 2). At zero frequency (or infinite deformation time scale) the complex modulus is
Hydration of magnesia cubes: a helium ion microscopy study
Beilstein J. Nanotechnol. 2016, 7, 302–309, doi:10.3762/bjnano.7.28
- (d–f) 4 days in the HIM chamber (chamber pressure p < 3·10−7 mbar). Significant volume increase, rounding of the edges, and partial fusion of the particles was observed (see arrows in micrographs). (HIM SE images digitally magnified from Figure 1.) Quantification of morphological changes: Curves
Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy
Beilstein J. Nanotechnol. 2016, 7, 220–227, doi:10.3762/bjnano.7.20
- to the electrode surface. Conclusion: We demonstrate here two platforms based on different sensing strategies as sensitive and selective tools to discriminate mismatches. Our assays are ready for parallelization and can be used in the detection and quantification of single nucleotide mismatches in
3D solid supported inter-polyelectrolyte complexes obtained by the alternate deposition of poly(diallyldimethylammonium chloride) and poly(sodium 4-styrenesulfonate)
Beilstein J. Nanotechnol. 2016, 7, 197–208, doi:10.3762/bjnano.7.18
- temperature [13]. In the last years, a lot of theoretical and experimental research effort has been spent to understand the different growth mechanisms that appear during the alternate deposition of the layers, the quantification of the adsorbed amount of material in each adsorption cycle, as well as the
pH-Triggered release from surface-modified poly(lactic-co-glycolic acid) nanoparticles
Beilstein J. Nanotechnol. 2015, 6, 2504–2512, doi:10.3762/bjnano.6.260
- commonly applied to detect anionic polymers [29] or dyes [30]. The obtained release data reveal valuable information about the pH-tunability of PAA in the complexed state within a polyelectrolyte multilayer. The quantification of released PDADMAC is a direct evidence of PEM decomposition and marks their
- . Typical broad polymer resonances are observed, where two characteristic signals for PDADMAC occur at chemical shifts of 3.15 and 3.25 ppm in a region where all other polymers show now signal, which makes a quantification of PDADMAC possible using 1H spectra. Quantification of PEI or PAA is difficult due
- to the comparatively lower signal intensities and some spectral overlap. The reference spectrum of resorcinol (data not shown) shows only chemical shifts higher than 4.8 ppm, which do not interfere with any polymer signal. For quantification, the integral of the resorcinol signal at a chemical shift
A simple and efficient quasi 3-dimensional viscoelastic model and software for simulation of tapping-mode atomic force microscopy
Beilstein J. Nanotechnol. 2015, 6, 2233–2241, doi:10.3762/bjnano.6.229
- quantification of tip–sample dissipation in atomic force microscopy (AFM) has been an ongoing subject of interest since the early days of the technique [1][2]. A significant percentage of the surfaces characterized with AFM exhibit rate-dependent deformation processes that result in dissipative tip–sample
Au nanoparticle-based sensor for apomorphine detection in plasma
Beilstein J. Nanotechnol. 2015, 6, 2224–2232, doi:10.3762/bjnano.6.228
- plasma To prove the applicability of the proposed method to the detection and quantification of APO in blood samples, a few experiments were performed on unfiltered blood plasma with different APO concentrations. These challenging conditions were chosen to minimize sample preparation, while still
Selective porous gates made from colloidal silica nanoparticles
Beilstein J. Nanotechnol. 2015, 6, 2105–2112, doi:10.3762/bjnano.6.215
- . Quantification was performed with external calibration curves (R2 ≥ 0.9999 for MB and R2 ≥ 0.9998 for RNAse, see Figures S1 and S2 in Supporting Information File 1). Additionally, some tests were performed applying an external stimulus (i.e., electric field) by using a battery of 9 V connected with two graphite
Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles
Beilstein J. Nanotechnol. 2015, 6, 1840–1852, doi:10.3762/bjnano.6.187
- recognition sites of the immunosensor. The plot of potential variation vs the amount of Ag gives a straight line (R2 = 0.97871) (Figure 4d) which confirmed the reproducibility of the measurements [35]. The limit of detection (LoD) and the limit of quantification (LoQ) were calculated using the formulae LoD
Analysis of soil bacteria susceptibility to manufactured nanoparticles via data visualization
Beilstein J. Nanotechnol. 2015, 6, 1635–1651, doi:10.3762/bjnano.6.166
- LRA biplots and simplified NMDSs with quantification provided by the distance correlation between MNP impacts summarized at different taxonomic levels. The present study demonstrates that visual exploration could potentially assist in knowledge discovery and interpretation of data on soil bacterial
The eNanoMapper database for nanomaterial safety information
Beilstein J. Nanotechnol. 2015, 6, 1609–1634, doi:10.3762/bjnano.6.165
- association of a 105-member library of surface-modified gold nanoparticles (see Figure 3). 785 distinct serum proteins were identified by LC-MS/MS, from which 129 were suitable for relative quantification. The fingerprint of serum proteins was defined by the relative abundance of each protein on a
Peptide-equipped tobacco mosaic virus templates for selective and controllable biomineral deposition
Beilstein J. Nanotechnol. 2015, 6, 1399–1412, doi:10.3762/bjnano.6.145
- shown in Table 3, the normalized intensities of two silica-derived fragments obtained with SIMS allow for a rough but reasonable quantification of the conversion of TEOS to silica induced by bare and KD10-functionalized TMV particles. While all negative controls not exposed to TEOS show negligible
PLGA nanoparticles as a platform for vitamin D-based cancer therapy
Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135
- performed in propidium iodide (PI)-stained S2-013, hTERT-HPNE and A549 cells after treatment with free calcitriol and calcitriol-loaded PLGA NPs at 1.2 µM for 72 h. PI counterstaining was used for DNA quantification. The differences in the DNA content between the cell population allowed the cell cycle
- TEM visualization [42]. Vitamin loading capacity (LC) and the encapsulation efficiency (EE) of PLGA NPs were further indirectly determined. For the quantification of the free vitamin, the NP suspension was centrifuged (14500 rpm, 30 min), and the supernatant analyzed. This step was conducted before
Scanning reflection ion microscopy in a helium ion microscope
Beilstein J. Nanotechnol. 2015, 6, 1125–1137, doi:10.3762/bjnano.6.114
- composition. A simple geometrical analysis of the reflection process was performed together with a Monte Carlo simulation of the angular dependence of the reflected ion yield. An interpretation of the RIM image formation and a quantification of the height of the surface steps were performed. The minimum
Simulation tool for assessing the release and environmental distribution of nanomaterials
Beilstein J. Nanotechnol. 2015, 6, 938–951, doi:10.3762/bjnano.6.97
- . In such analysis, the environmental entry, movement, and distribution of contaminants are described by a set of mathematical expressions. Specifically, MCMs require mechanistic quantification of intermedia transport rates (e.g., dry and wet deposition, sedimentation, dissolution) and rates of
Entropy effects in the collective dynamic behavior of alkyl monolayers tethered to Si(111)
Beilstein J. Nanotechnol. 2015, 6, 583–594, doi:10.3762/bjnano.6.60
- -alkanes (44 meV) [57]. However, an accurate quantification of the number of gauche defects would require an estimate of the dipole moments for all non-centrosymmetric methylene conformations, which will depend on details of the trans–gauche sequences. Vibrational spectroscopy and molecular dynamics
Pulmonary surfactant augments cytotoxicity of silica nanoparticles: Studies on an in vitro air–blood barrier model
Beilstein J. Nanotechnol. 2015, 6, 517–528, doi:10.3762/bjnano.6.54
- quantification method for determination of the number of viable cells is cell staining with crystal violet (CV, purchased from Merck, 1407) [19]. Crystal violet (N-hexamethyl pararosaniline) is a monochromatic dye which stains cell nuclei. After fixation of NP-exposed cells they were incubated with 50 μL/96er
- three aSNPs after an incubation time of 4 h in serum-free medium. An approximate quantification of cellular uptake via fluorescence intensity measurement of the images could not be conducted due to the variable fluorescence intensity of the aSNP labeling itself. Comparing all three aSNPs using same
- approximate uptake quantification via fluorescence intensity measurements was not suitable due to the different fluorescence-labeling intensities of the aSNPs themselves. The decrease of MTS conversion at lower concentrations of aSNP–COOH remains unexplained at the moment. The fact that in this study no cell
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