The distribution and degradation of radiolabeled superparamagnetic iron oxide nanoparticles and quantum dots in mice

• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Markus Heine,
• Christian Waurisch,
• Gordon M. Stachowski,
• Stephen G. Hickey,
• Alexander Eychmüller,
• Jörg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 111–123, doi:10.3762/bjnano.6.11

• normal diet of 260 µg Zn [35][36][37]. Size-exclusion chromatography (SEC) Similar to that previously described in [24], SEC was performed using a Superose-6 10/300 GL column (Amersham Bioscience, Munich, Germany) with buffer (10 mM tris(hydroxymethyl)aminomethane, 0.15 mM NaCl, 10 mM EDTA) at a flow

The fate of a designed protein corona on nanoparticles in vitro and in vivo

• Denise Bargheer,
• Julius Nielsen,
• Gabriella Gébel,
• Markus Heine,
• Sunhild C. Salmen,
• Roland Stauber,
• Horst Weller,
• Joerg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 36–46, doi:10.3762/bjnano.6.5

• (hydroxymethyl)aminomethane, pH 8.0; 0.15 mM NaCl, 10 mM EDTA) at a flow rate of 0.5 mL/min. Under UV 280 nm detection, fractions of 0.5 mL were collected and 125I and 59Fe were measured using a γ-counter. The column was calibrated by injecting a sample of human plasma and DLS-measurements were performed in

• injection the torso was opened and blood was removed from the right ventricle using an EDTA-coated syringe. The right atrium was opened and PBS containing 10 units of heparin was perfused via the left ventricle for 3 min. Then, the organs (spleen, kidney, liver, etc.) were harvested, weighted and the

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• agent ethylenediaminetetraacetic acid (EDTA, 2 mL) for 10 min in the incubator. Then, EDTA was removed and cells were detached from the substrate by incubation with trypsin/EDTA (1 mL) for 10 min in the incubator. Trypsination was stopped by addition of medium (10 mL), which was removed afterward by

The gut wall provides an effective barrier against nanoparticle uptake

• Heike Sinnecker,
• Thorsten Krause,
• Sabine Koelling,
• Ingmar Lautenschläger and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2092–2101, doi:10.3762/bjnano.5.218

• harvested by first flushing the gut with air and then gently squeezing the tissue with moist cotton pads. Mucus was dissolved in a lysis buffer (100 mM Tris-Cl, pH 8.0–8.5, 200 mM NaCl, 0.2% SDS, 5 mM EDTA, 100 µg/mL proteinase K) for 15 to 30 min, and the fluorescence of the solubilized effluate was

Effect of silver nanoparticles on human mesenchymal stem cell differentiation

• Christina Sengstock,
• Jörg Diendorf,
• Matthias Epple,
• Thomas A. Schildhauer and
• Manfred Köller

Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214

• Technologies) and detached from the culture flasks by the addition of 0.2 mL·cm−2 0.25% trypsin/0.05% ethylenediaminetetraacetic acid (EDTA, Sigma-Aldrich, Taufkirchen, Germany) for 5 min at 37 °C. Subsequently, the hMSCs were collected and washed twice with RPMI1640/10% FCS. Determination of cellular Ag-NP

Rapid degradation of zinc oxide nanoparticles by phosphate ions

• Rudolf Herrmann,
• F. Javier García-García and
• Armin Reller

Beilstein J. Nanotechnol. 2014, 5, 2007–2015, doi:10.3762/bjnano.5.209

• centrifuged again. This washing was repeated two more times. The final product was redispersed in 1.5 mL of ethanol, and 0.1 mL were used for TEM measurements. From the remaining solution the ethanol was evaporated and the residue was dissolved in 10 mL of 0.01 N HCl and 10 mL of an aqueous solution of EDTA

Real-time monitoring of calcium carbonate and cationic peptide deposition on carboxylate-SAM using a microfluidic SAW biosensor

• Anna Pohl and
• Ingrid M. Weiss

Beilstein J. Nanotechnol. 2014, 5, 1823–1835, doi:10.3762/bjnano.5.193

• 8.2 and pH 9.0 were performed analogous on the same biosensor chip using separate channels. The flow rate was 40 µL/min and the running buffer was the respective Gly–Gly buffer. Between the injections of the peptides EDTA was injected. Volumes of 200 µL were injected if not otherwise indicated. The

• . Ethylendiamintetraacetate (EDTA) solution For regenerating the sensor chip after exposure to calcium carbonate, 1 mM and 10 mM EDTA solutions were prepared from a 0.5 M EDTA stock solution, pH 8.0 (146 g/L). Glycerol solution For determining viscosity effects, a 5% glycerol solution was prepared in aqueous buffer. Phase

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

• Dagmar A. Kuhn,
• Dimitri Vanhecke,
• Benjamin Michen,
• Fabian Blank,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

• , Switzerland) and 1% penicillin/streptomycin (Gibco, Luzern, Switzerland) and kept at 37 °C and 5% CO2. The A549 epithelial cells were split twice per week using trypsin (0.05% trypsin-EDTA, GIBCO, Switzerland). J774A.1 cells were sub-cultured using the scraping method, resuspended in the medium and finally

Donor–acceptor graphene-based hybrid materials facilitating photo-induced electron-transfer reactions

• Anastasios Stergiou,
• Georgia Pagona and
• Nikos Tagmatarchis

Beilstein J. Nanotechnol. 2014, 5, 1580–1589, doi:10.3762/bjnano.5.170

• EDTA and re-complexes after fresh addition of Fe(II) (Scheme 2). Furthermore, Fe–tpy–GO was tested as a catalyst for the oxygen reduction reaction (ORR) and found to be durable against carbon monoxide poisoning and to exhibit a higher fuel selectivity compared with commercially available Pt/C electro

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• regenerating the binding capacity of the artificial membranes by dissociating the integrin–ECM ligand complexes with ethylenediaminetetraacetate (EDTA). They concluded that integrin-functionalised membranes are well qualified as sensing devices for the detection of sensible ligand–receptor interactions. 2.2

Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe₂O₃ nano-sized particles and assessment of their effects on glutamate transport

• Tatiana Borisova,
• Natalia Krisanova,
• Arsenii Borуsov,
• Roman Sivko,
• Ludmila Ostapchenko,
• Michal Babic and
• Daniel Horak

Beilstein J. Nanotechnol. 2014, 5, 778–788, doi:10.3762/bjnano.5.90

• animals were removed and homogenized rapidly in ice-cold 0.32 M sucrose, 5 mM HEPES–NaOH, pH 7.4, and 0.2 mM EDTA. The synaptosomes were prepared by differential and Ficoll-400 density gradient centrifugation of rat brain homogenate [31] with slight modifications [30]. All manipulations were performed at

Biocalcite, a multifunctional inorganic polymer: Building block for calcareous sponge spicules and bioseed for the synthesis of calcium phosphate-based bone

• Xiaohong Wang,
• Heinz C. Schröder and
• Werner E. G. Müller

Beilstein J. Nanotechnol. 2014, 5, 610–621, doi:10.3762/bjnano.5.72

• specific activity of about 5,000 units/mg, was added at a concentration of 35 W-A units (10 μg)/500 μL of CaCl2 to the assays. The formation of calcium carbonate was determined quantitatively on the basis of the consumption of free Ca2+ ions using the EDTA titration procedure [31]. The assays either

Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating

• Tingjun Lei,
• Alicia Fernandez-Fernandez,
• Romila Manchanda,
• Yen-Chih Huang and
• Anthony J. McGoron

Beilstein J. Nanotechnol. 2014, 5, 313–322, doi:10.3762/bjnano.5.35

• -Aldrich (St. Louis, MI): Malic acid, 1,12-dodecanedioic acid (DDA), dimethylsulfoxide (DMSO > 99.9%, reagent grade), pluronic F-127, Dulbecco phosphate-buffered saline (DPBS), phosphate buffered saline (PBS), IR820, penicillin-streptomycin solution, tetrahydrofuran (THF) and trypsin-EDTA. Glycerol was

Ultramicrosensors based on transition metal hexacyanoferrates for scanning electrochemical microscopy

• Maria A. Komkova,
• Angelika Holzinger,
• Andreas Hartmann,
• Alexei R. Khokhlov,
• Christine Kranz,
• Arkady A. Karyakin and
• Oleg G. Voronin

Beilstein J. Nanotechnol. 2013, 4, 649–654, doi:10.3762/bjnano.4.72

• achieved. However, the stability of PB in neutral aqueous solutions is not sufficient in respect to the long-term continuous monitoring of high levels of H2O2 [20]. Moreover, different iron complexing agents (e.g., EDTA) are known to solubilize PB. This problem is particularly severe for PB-modified UME

Forming nanoparticles of water-soluble ionic molecules and embedding them into polymer and glass substrates

• Stella Kiel,
• Olga Grinberg,
• Nina Perkas,
• Jerome Charmet,
• Herbert Kepner and
• Aharon Gedanken

Beilstein J. Nanotechnol. 2012, 3, 267–276, doi:10.3762/bjnano.3.30

• complexometric EDTA reagent [15]. The structure of the deposited nanoparticles was characterized by XRD on a Bruker D8 diffractometer (with Cu Kα = 1.5418 Å radiation). The size of the particles in the working solution was controlled by DLS measurements using a Coulter particle analyzer (Malvern Zetasizer). The

• sonication, volumetric titration according to Mohrs method (Cl−) [14] and complexometric titration with EDTA reagent (Cu2+) [15] were performed. The titrations were carried out for the two types of solutions: The solutions that were sonicated without the immersed glass slides and the solutions that were