Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells

• Michal Babič,
• Daniel Horák,
• Lyubov L. Lukash,
• Tetiana A. Ruban,
• Yurii N. Kolomiets,
• Svitlana P. Shpylova and
• Oksana A. Grypych

Beilstein J. Nanotechnol. 2014, 5, 1732–1737, doi:10.3762/bjnano.5.183

• the modified particles were characterized by transmission electron microscopy and dynamic light scattering with regard to morphology, particle size and polydispersity. In vitro survival of human stem cells was then investigated by using the methyl thiazolyl tetrazolium (MTT) assay, which showed that D

• -mannose- and poly(N,N-dimethylacrylamide)-coated γ-Fe2O3 particles exhibit much lower level of cytotoxicity than the non-coated γ-Fe2O3. Keywords: maghemite; magnetic; MTT assay; nanoparticles; stem cells; Introduction One of the most important applications of nanoparticles in biomedicine is the direct

• zero remanent magnetization of the particles, the risk of formation of aggregates in physiological liquids is reduced. MTT assay In order to achieve an efficient cell labeling, the response of intracellular γ-Fe2O3 content on the metabolism of the cells should be taken into account because the

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• OVA > BGG > LYS > BSA > BHG > HIS, OVA > BGG > LYS > BHG > BSA > HIS and OVA > BGG > BSA > BHG > LYS > HIS for the HCT116, HeLa and SCC-7 cell lines, respectively (Table S3, Supporting Information File 1). The MTT assay on the blank proteins showed cell viabilities greater than 80% (Figure S7

• cytotoxicity studies. The reactions were also carried out in the absence of AgNO3, keeping all of the other conditions the same as above. Cell culture and cell viability test (MTT assay) In a similar manner to the methods described in [61], Mouse embryonic fibroblasts (NIH-3T3), human colorectal cancer cells

• and (F) BGG. Scale bar: 20 nm. Variation in the zeta potential of AuNPs prepared by using different proteins as a function of the pH in aqueous medium. Cell viability studies by using MTT assay on (A) NIH-3T3, (B) HCT116, (C) HeLa and (D) SCC-7 cells treated with AuNPs prepared by using different

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

• Dominic Docter,
• Christoph Bantz,
• Dana Westmeier,
• Hajo J. Galla,
• Qiangbin Wang,
• James C. Kirkpatrick,
• Peter Nielsen,
• Michael Maskos and
• Roland H. Stauber

Beilstein J. Nanotechnol. 2014, 5, 1380–1392, doi:10.3762/bjnano.5.151

• MTT assay (Figure 6). Similar to the lung surfactant [32], also epithelial cell of the GI tract are covered by an additional biobarrier, i.e., by mucous matrices [33]. To investigate the impact of mucus associated to GI tract cells on the observed effects, we included the mucus-secreting colorectal

• 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as described in [54]. Briefly, following NP exposure, cells were incubated with MTT (400 μg/mL; 965 μM; Life Technologies, Carlsbad, USA) for 4 h. The MTT was removed, the cells were washed with PBS and solubilized in dimethyl

• by using the MTT assay. Data are depicted as percentage compared to untreated control cells, which was set to 100% vitality. Results are shown as means ± SD (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001. Automated microscopy to analyze the impact of exposure to ASP30 on the cell viability. Caco-2

Magnetic-Fe/Fe₃O₄-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages

• Hongwang Wang,
• Tej B. Shrestha,
• Matthew T. Basel,
• Raj K. Dani,
• Gwi-Moon Seo,
• Sivasai Balivada,
• Marla M. Pyle,
• Heidy Prock,
• Olga B. Koper,
• Prem S. Thapa,
• David Moore,
• Ping Li,
• Viktor Chikan,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2012, 3, 444–455, doi:10.3762/bjnano.3.51

• of the nanoparticles in the cells and compared to the side scatter of control cells. 10,000 cells were counted and analyzed. This procedure was repeated three times. Data were analyzed by using Cytosoft software (Guava Easycyte Plus System, Millipore Corporation, MA). MTT Assay The MTT assay [46] was

• . Different concentrations of nanoparticles were taken up by double-stable Mo/Ma cells over 24 h; the nanoparticle-concentration ranged from 0 to 320 μg/mL MNP-SN38 in fresh medium. After 24 h, the inhibition of cell proliferation was measured by using the MTT assay (Figure 4). We found only 20% of inhibition

• of nanoparticles contained 0.427 mg of iron, indicating that this amount of iron would be high enough for alternating magnetic field hyperthermia in combination with chemotherapy [54]. The MTT assay indicated that 8 pg of iron can be easily loaded in each cell (20% inhibition of cell proliferation