Use of nanosystems to improve the anticancer effects of curcumin

• Andrea M. Araya-Sibaja,
• Norma J. Salazar-López,
• Krissia Wilhelm Romero,
• José R. Vega-Baudrit,
• J. Abraham Domínguez-Avila,
• Carlos A. Velázquez Contreras,
• Ramón E. Robles-Zepeda,
• Mirtha Navarro-Hoyos and
• Gustavo A. González-Aguilar

Beilstein J. Nanotechnol. 2021, 12, 1047–1062, doi:10.3762/bjnano.12.78

• molecular targeting properties [40]. An LPHN was co-loaded with CUR and with docetaxel (82% and 90% average EE, respectively) and assayed in mice bearing PC3 (human prostate cancer) tumor xenografts [87]. The results showed higher cytotoxicity and a synergistic effect, which resulted in tumor growth

Hybrid Au@alendronate nanoparticles as dual chemo-photothermal agent for combined cancer treatment

• Anouchka Plan Sangnier,
• Romain Aufaure,
• Laurence Motte,
• Claire Wilhelm,
• Erwann Guenin and
• Yoann Lalatonne

Beilstein J. Nanotechnol. 2018, 9, 2947–2952, doi:10.3762/bjnano.9.273

• under irradiation within the first biological window (650–900 nm). The Au@alendronate nanoplatform thus provided a combined antitumor activity through drug delivery and photothermal therapy. Au@alendronate NPs inhibited in vitro the proliferation of prostate cancer cells (PC3) in a dose-dependent manner

• physiological pH values and their photothermal properties within the NIR first biological window allowed us to further consider their study in a biological environment. Au@alendronate NPs antitumor activity PC3 human prostate adenocarcinoma cells were selected to explore the potential of Au@alendronate NPs as

• antitumor agents [9]. PC3 cells were first treated both with free alendronate and with Au@alendronate NPs (at various extracellular alendronate concentrations from 1 nM up to 0.1 M) for 48 h. Metabolic activity (Figure 3) was determined by Alamar Blue assay (see Supporting Information File 1). With this

Surface-enhanced Raman spectroscopy of cell lysates mixed with silver nanoparticles for tumor classification

• Mohamed Hassoun,
• Iwan W.Schie,
• Tatiana Tolstik,
• Sarmiza E. Stanca,
• Christoph Krafft and
• Juergen Popp

Beilstein J. Nanotechnol. 2017, 8, 1183–1190, doi:10.3762/bjnano.8.120

• signals from 2800 to 3000 cm−1. The most pronounced spectral features were (i) positive bands near 660, 900 and 2900 cm−1 in PC2 loadings, (ii) a derivative-like feature at 660 cm−1 and negative bands near 723 and 1339 cm−1 in PC3 loadings, and (iii) negative band near 660 and derivative-like feature near

• are plotted in Figure 5. Based on four PCs the main variations between the four cell lines were explained, and cells could be differentiated. Negative PC1 scores separated the spectra of the MCF-7 cell line from the spectra of the other cell lines having positive PC1 score values. PC2, PC3 and PC4

Multiwalled carbon nanotube hybrids as MRI contrast agents

• Nikodem Kuźnik and
• Mateusz M. Tomczyk

Beilstein J. Nanotechnol. 2016, 7, 1086–1103, doi:10.3762/bjnano.7.102

• according to their magnetic moment (and the content of iron), so the researchers were able to separate mechanically the most iron-enriched hybrids from the rest of the product. Another example comes from Lamanna's research, where enhanced uptake of CNTs by PC3 tumor cells directly above a neodymium magnet

• period, is consistent with the abovementioned toxicity studies. Cytotoxicity and hemolysis Cytotoxicity of the nanohybrids was studied on various cell types (HeLa, HEK 293, human prostate cancer cells PC3, fibroblasts and others) and a general conclusion is the dose-dependent trend. However, the

Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces

• Miao Yu,
• Nico Strohmeyer,
• Jinghe Wang,
• Daniel J. Müller and
• Jonne Helenius

Beilstein J. Nanotechnol. 2015, 6, 157–166, doi:10.3762/bjnano.6.15

• allowed for experiments that previously were not feasible. Since the masks are economical and versatile, they can aid in the improvement of various assays. Keywords: atomic force microscopy; cell adhesion; collagen I; fibroblasts; fibronectin; HeLa; laminin; MDCK; PC3; single cell assay; single cell

• -specific cell adhesion to clean glass is higher than the adhesion of cells to ConA-coated cantilevers. In order to attach cells to ConA-coated cantilevers, it was necessary to passivate glass surfaces onto which cells were pipetted. We found that both HeLa and PC3 cells also strongly adhere to PDMS (Figure

•  4). Therefore, it was necessary to passivate the PDMS surfaces. BSA is commonly used to block non-specific adhesion of cells to different surfaces [38]. To address non-specific cell adhesion, we coated glass and PDMS wells with BSA. To our surprise, the adhesion of PC3 cells was higher for BSA when

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• nanoparticle infiltration into a tissue lesion or by insertion of nanoparticle patterned implants. This can potentially influence cell migration, which has implications in wound healing [13]. Recently, a study by Yang et al. tracked the migration behavior of prostate carcinoma cells (PC3, epithelial) and human

• optical dark field microscopy. No pronounced cell migration away from the initial adhesion area or nanoparticle removal from the substrate was observed in contrast to previous reports on 3T3 fibroblast cells [15] or prostate carcinoma (PC3) and human dermal fibroblast (HDF) cells [14]. However, in this

Nanodiamond-DGEA peptide conjugates for enhanced delivery of doxorubicin to prostate cancer

• Amanee D Salaam,
• Patrick Hwang,
• Roberus McIntosh,
• Hadiyah N Green,
• Ho-Wook Jun and
• Derrick Dean

Beilstein J. Nanotechnol. 2014, 5, 937–945, doi:10.3762/bjnano.5.107

• tissues, the ND-DGEA conjugates were designed to distinguish between cells that overexpress α2β1 integrin, bone metastatic prostate cancers cells (PC3), and cells that do not, human mesenchymal stem cells (hMSC). Utilizing the ND-DGEA+DOX system, the efficacy of 1 µg/mL and 2 µg/mL DOX doses increased

• collagens, laminins, E-cadherin, and matrix metalloproteinase 1 [31]. α2β1 integrins have been proven to be up-regulated in bone metastatic prostate cancer cells [32][33]. Particularly, PC3 human bone metastatic prostate cancer cell lines have the highest expression of α2β1 integrins when compared to other

• metastatic cell lines CWR-22 and LNCaP [31]. The over-expression of α2β1 integrins in PC3 can be harnessed as a target for a drug delivery platform. The toxicity of DOX can be decreased by increasing the interaction between the drug and cancer cells. Since α2β1 integrins are over-expressed in bone metastatic