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Search for "RNA" in Full Text gives 61 result(s) in Beilstein Journal of Nanotechnology.
Modification of a single-molecule AFM probe with highly defined surface functionality
Beilstein J. Nanotechnol. 2014, 5, 2122–2128, doi:10.3762/bjnano.5.221
- double-stranded RNA and DNA molecules [12]. Despite all the success in these studies, one significant challenge remains in AFM-based SMFS, that is to attach only one or a few molecules to the AFM probes. In previous studies, the most common method depended on non-specific adhesions [13][14], which
Effect of channel length on the electrical response of carbon nanotube field-effect transistors to deoxyribonucleic acid hybridization
Beilstein J. Nanotechnol. 2014, 5, 2081–2091, doi:10.3762/bjnano.5.217
- variation trend in response to the newly defined parameters. Keywords: biosensor; carbon nanotubes; channel length; field-effect transistor; hybridization; mobility; nucleic acid; Introduction Detection of nucleic acids such as deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) is an important issue in
The cell-type specific uptake of polymer-coated or micelle-embedded QDs and SPIOs does not provoke an acute pro-inflammatory response in the liver
Beilstein J. Nanotechnol. 2014, 5, 1432–1440, doi:10.3762/bjnano.5.155
- RNA was isolated and cDNA was prepared according to the manufacturer’s instructions (Applied Biosystems). Real-time quantitative RT-PCR was performed by using assay-on-demand primer/probe sets supplied by Applied Biosystems (assay IDs are available upon request). Relative expression was calculated by
Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches
Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149
- could be observed when simultaneously exposed with Ag NPs. Ag NPs were not found to interfere with the ELISA assay (data not shown). Gene expression of pro-inflammatory and oxidative stress markers As described in [44], the total RNA content of the triple cell co-cultures was collected 4 and 24 h after
- RNAprotect cell reagent (Qiagen AG, Hombrechtikon, Switzerland) and stored at 4 °C until further processing. Cells were detached by vortexing and lysed by centrifuging with QIAshredder columns (Qiagen). Total RNA was isolated by using the RNeasy plus kit (Qiagen) according to the manufacturer’s guidelines
- and the RNA concentration was determined by a NanoDrop 2000 (Thermo Scientific, Witec AG, Littau, Switzerland). Reverse transcription (incubation 1 h at 37 °C) was carried out with the Omniscript Reverse Transcription Kit (Qiagen) in 10 µL volume with 0.25 µg RNA/reaction, by using a master mix
Molecular biology approaches in bioadhesion research
Beilstein J. Nanotechnol. 2014, 5, 983–993, doi:10.3762/bjnano.5.112
- hybridization screen, and (4) functional analyses of selected genes by using RNA interference knock-down. Furthermore, latest developments in genome-editing are presented as new tools to study gene function. By using this iterative multi-technologies approach, the identification, isolation, expression and
- expression; in situ hybridization; RNA interference; transcriptome; Introduction The capability of an organism to attach to a surface, either temporarily or permanently, is referred to as “bioadhesion”. Bioadhesion occurs in many living organisms that have designed ways to adhere to a range of surfaces [1
- isolation of genes, and the study of their expression and function (Figure 1). Methods in the field have advanced tremendously in recent years, largely due to the recent advances in DNA and RNA sequencing, and protein analysis. These technologies allow research objectives to move from the analyses of single
Injection of ligand-free gold and silver nanoparticles into murine embryos does not impact pre-implantation development
Beilstein J. Nanotechnol. 2014, 5, 677–688, doi:10.3762/bjnano.5.80
- , AgNP injected, sham injected, handling control. Blastocysts were pooled in groups of ten. Per treatment group, 7 pools were examined. Pools were lysed in 40 µL of lysis-binding buffer, then 1 pg of rabbit globin mRNA (BRL, Gaithersburg, MD) was added as an external standard. Poly(A)+-RNA was prepared
Apertureless scanning near-field optical microscopy of sparsely labeled tobacco mosaic viruses and the intermediate filament desmin
Beilstein J. Nanotechnol. 2013, 4, 510–516, doi:10.3762/bjnano.4.60
- and might give insight in the patho-mechanism. The tobacco mosaic virus (TMV) is a tubular shaped plant virus with a length of 300 nm and a diameter of 18 nm. It is composed of 2130 identical capsid protein subunits helically arranged on a single RNA strand thereby enclosing an inner longitudinal
Size variation of infrared vibrational spectra from molecules to hydrogenated diamond nanocrystals: a density functional theory study
Beilstein J. Nanotechnol. 2013, 4, 262–268, doi:10.3762/bjnano.4.28
- sometimes used in solid-state physics and nanocrystals [21][24]. It is also used in large molecules, such as RNA [25][26]. Conclusion As concluding remarks, we can note that the present theory can adequately reproduce many of the experimental data of infrared vibrational frequencies. This includes the 330
Electrospinning preparation and electrical and biological properties of ferrocene/poly(vinylpyrrolidone) composite nanofibers
Beilstein J. Nanotechnol. 2013, 4, 189–197, doi:10.3762/bjnano.4.19
- , there are a series of chemical reactions between Fc and the enzymes, DNA and RNA in the cell due to the penetration of Fc, leading to cell death [32]. The Fc conveys many unique properties such as low toxicity, stability, lipophilicity, aromaticity and easy access to one-electron oxidation potential
FTIR nanobiosensors for Escherichia coli detection
Beilstein J. Nanotechnol. 2012, 3, 485–492, doi:10.3762/bjnano.3.55
- 1402–1457 cm−1, bands due to carbohydrates, glycoproteins, lipids and their characteristic C–O–H in-plane bending peaks and C(CH3)2 symmetric stretching were present. Finally, in the range 900–1100 cm−1, peaks due to the DNA/RNA backbone and phosphate groups of nucleic acids due to the symmetric and
Distinction of nucleobases – a tip-enhanced Raman approach
Beilstein J. Nanotechnol. 2011, 2, 628–637, doi:10.3762/bjnano.2.66
- the respective nucleobases is possible, and this eventually led to successful TERS measurements on a single RNA strand of a cytosine homopolymer [19]. The dependency of the electromagnetic field enhancement of TERS on the composition of the substrate, amongst other parameters, was shown in three
- , and hence in this case the effect relies on an isolated tip, resulting in a much smaller enhancement [20][21]. Prerequisites for a metal substrate suitable for TERS experiments on single stranded DNA or RNA are an almost atomic flatness of the surface and, as the used TERS setup is operated in back
- performing TERS experiments on native DNA [14]. In contrast, TERS measurements on calf thymus DNA presented here clearly show that specific contributions of all four nucleobases can be determined. Hence, with the chosen samples poly(A), poly(U) and calf thymus DNA typical molecules covering DNA, RNA and
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