Beilstein J. Nanotechnol.2011,2, 374–383, doi:10.3762/bjnano.2.43
, namely serum albumin, apolipoprotein A-I and apolipoprotein E4, onto polymer-coated, fluorescently labeled FePt nanoparticles (~12 nm diameter) carrying negatively charged carboxyl groups on their surface. For all three proteins, a step-wise increase in hydrodynamic radius with protein concentration was
correlation spectroscopy; human serum albumin; nanoparticle; protein adsorption; Introduction
Recent years have seen enormous advances in the field of nanotechnology. A huge variety of nanoparticles (NPs), defined as objects with all three spatial dimensions in the range of 1–100 nm, has been developed, with
understand the structural and dynamic properties of the protein corona at the molecular level. Recently, we have used quantitative fluorescence microscopy, especially fluorescence correlation spectroscopy (FCS), to study protein adsorption of human serum albumin (HSA) on polymer-coated FePt NPs with an
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Figure 1:
Fluorescence intensity correlation curves of NPs dissolved in buffer solutions of (a, b) HSA, (c, d...
Beilstein J. Nanotechnol.2010,1, 142–154, doi:10.3762/bjnano.1.17
bovine serum albumin (BSA) protein as a control did not elicit any change in T2 (Figure 5b). More recently, MRSw biosensors, capable of detecting soluble tumor biomarker proteins (such as CA-125, VEGF, and α-fetoprotein) were described, and used for parallel detection of multiple markers in blood samples
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Figure 1:
DMR assay configurations with magnetic nanoparticles (MNPs). (a) Magnetic relaxation switching (MRS...