Beilstein J. Nanotechnol.2017,8, 715–722, doi:10.3762/bjnano.8.75
arrays we selected lipid–protein pairs applied in previous settings: Biotin-Cap-PE and streptavidin labeled with Cy3 dye (STV-Cy3) as a simple protein model; and DNP-cap-PE with anti-DNP IgE as a model for allergen/antibody recognition. These interactions are well-characterized for biomimetic lipid
over the whole arrays, indicating an even distribution of biotin headgroups embedded in the nanoporous HEMA-EDMA (Figure 2).
To assess the accessibility of lipid based allergen arrays on nanoporous HEM-EDMA A substrates to antibodies, we employed the established lipid/antibody pair DNP-cap-PE and anti
-DNP IgE antibody. Previously, DNP-lipid recognition by a specific antibody was tested on glass supports [17], while on HEMA-EDMA polymer antibody binding was tested on covalently bound DNP-azide ink [10]. Here, allergen arrays were prepared by using 10 mol % admixture of DNP-cap-PE into DOPC lipid. As
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Figure 1:
Phospholipid array on nanoporous HEMA-EDMA polymer. a) Phospholipid microcontact spotting on porous...
Beilstein J. Nanotechnol.2017,8, 244–253, doi:10.3762/bjnano.8.27
approach, our data showed a sensitivity increase of at least five times and a lower detection limit with respect to a standard ELISA of at least three times. Additionally, the assay time was remarkably decreased.
Keywords: allergen; ELISA enhancement; functionalization; gliadin; gold nanoparticle
antibodies and HRP on AuNPs. Many of the allergen determinations by ELISA use this strategy, but more research on this is necessary to confirm this.
Conclusion
In summary, our main objective at the start of the work was to elucidate whether a covalent loading or directional binding of biomolecules on AuNPs
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Figure 1:
Schematic representation of the four different functionalization methods explored in this work. (a)...