Fusion of purple membranes triggered by immobilization on carbon nanomembranes

• René Riedel,
• Natalie Frese,
• Fang Yang,
• Martin Wortmann,
• Raphael Dalpke,
• Daniel Rhinow,
• Norbert Hampp and
• Armin Gölzhäuser

Beilstein J. Nanotechnol. 2021, 12, 93–101, doi:10.3762/bjnano.12.8

• electron irradiation-induced cross-linking of a self-assembled monolayer (SAM) of 4′-nitro-1,1′-biphenyl-4-thiol (NBPT) and second, purple membrane (PM) containing genetically modified bacteriorhodopsin (BR) carrying a C-terminal His-tag. The NBPT-CNM was further modified to carry nitrilotriacetic acid

• PM on a solid support. The resulting hybrid membrane has a potential application as a light-driven two-dimensional proton-pumping membrane, for instance, for light-driven seawater desalination as envisioned soon after the discovery of PM. Keywords: bacteriorhodopsin; carbon nanomembrane

• protein bacteriorhodopsin (BR) and lipid molecules [3], can be separated from the cytoplasmic membrane of Halobacterium salinarum and have been intensively studied [4][5][6]. Due to its robustness, PM quickly became one of the best-characterized natural membranes [7]. It is a two-dimensional crystal with

Materials nanoarchitectonics at two-dimensional liquid interfaces

• Katsuhiko Ariga,
• Michio Matsumoto,
• Taizo Mori and
• Lok Kumar Shrestha

Beilstein J. Nanotechnol. 2019, 10, 1559–1587, doi:10.3762/bjnano.10.153

Bacteriorhodopsin–ZnO hybrid as a potential sensing element for low-temperature detection of ethanol vapour

• Saurav Kumar,
• Sudeshna Bagchi,
• Senthil Prasad,
• Anupma Sharma,
• Ritesh Kumar,
• Rishemjit Kaur,
• Jagvir Singh and
• Amol P. Bhondekar

Beilstein J. Nanotechnol. 2016, 7, 501–510, doi:10.3762/bjnano.7.44

• - Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India, Research Services, University of Alberta, Edmonton, AB, Canada T6G2E1 10.3762/bjnano.7.44 Abstract Zinc oxide (ZnO) and bacteriorhodopsin (bR) hybrid nanostructures were fabricated by immobilizing bR on ZnO thin films and ZnO nanorods

• , nanomaterial–biomolecule hybrid gas sensors. Keywords: amphipol; bacteriorhodopsin; bio-hybrid; gas sensing; ITO; ZnO nanostructure; Introduction Nanomaterial–biomolecule conjugates have emerged into one of the most rapidly developing and sought after areas in modern biomolecular device fabrication and

• retention of functional properties ex vivo, and a lack of immobilization techniques to prevent denaturation [4][8]. Interestingly, the protein bacteriorhodopsin (bR) has been proven to have significant stability against thermal, chemical and photochemical degradation [11][12][13]. Also, bR maintains its

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• reviewed by P. Walde et al. [47]. Several membrane proteins have already been incorporated into GUVs over the past years. Girard and co-workers first reconstituted Ca2+-ATPase from the sarcoplasmic reticulum and the H+ pump bacteriorhodopsin into liposomes of 0.1 to 0.2 μm in size [48]. The liposomes were

• proteins like bacteriorhodopsin and the ferrichrome transport protein FhuA into GUVs with lipid mixtures representative of cellular plasma membranes [51]. Reconstitution was either performed with proteins solubilised in detergent micelles, with proteoliposomes or purified native membranes. This method