Targeting the vector of arboviruses Aedes aegypti with nanoemulsions based on essential oils: a review with focus on larvicidal and repellent properties

• Laryssa Ferreira do Nascimento Silva,
• Douglas Dourado,
• Thayse Silva Medeiros,
• Mariana Alice Gonzaga Gabú,
• Maria Cecilia Queiroga dos Santos,
• Daiane Rodrigues dos Santos,
• Mylena Lemos dos Santos,
• Gabriel Bezerra Faierstein,
• Rosângela Maria Rodrigues Barbosa and
• Fabio Rocha Formiga

Beilstein J. Nanotechnol. 2025, 16, 1894–1913, doi:10.3762/bjnano.16.132

• through a low-energy method, using polysorbate 20 as a surfactant. The formulation presented an average droplet size ranging from 244.6 to 280.4 nm, a polydispersity index of less than 0.25, and a negative zeta potential (−15.7 to −18.6 mV), maintaining stability for 14 days. In the bioassays, fourth

• by phase inversion, using polysorbate 80 and sorbitan monooleate 20 as surfactants. The NE presented an average size of 105.3 nm (±1.36) and a polydispersity index of 0.263 (± 0.004). The larvicidal bioassays against third-stage Aedes aegypti larvae produced an LC50 of 61.4 µg/mL at 48 hours and 26.8

• using a low-energy method with sorbitan monooleate 80 and polysorbate 20. The NE presented an average droplet size of 74.5 nm (±1.939) and a polydispersity index of 0.271 (±0.007), remaining physicochemically stable for up to 180 days. Larvicidal bioassays against third-stage Aedes aegypti larvae

Chitosan nanocomposite containing rotenoids: an alternative bioinsecticidal approach for the management of Aedes aegypti

• Maria A. A. Bertonceli,
• Vitor D. C. Cristo,
• Ivo J. Vieira,
• Francisco J. A. Lemos,
• Arnoldo R. Façanha,
• Raimundo Braz-Filho,
• Gustavo V. T. Batista,
• Luis G. M. Basso,
• Sérgio H. Seabra,
• Thalya S. R. Nogueira,
• Felipe F. Moreira,
• Arícia L. E. M. Assis,
• Antônia E. A. Oliveira and
• Kátia V. S. Fernandes

Beilstein J. Nanotechnol. 2025, 16, 1197–1208, doi:10.3762/bjnano.16.88

• with smaller size, improved polydispersity index, and enhanced stability, evidenced by a higher zeta potential. FTIR analysis confirmed rotenoid incorporation into the nanocomposite and suggested hydrogen bonding or potential covalent interaction with chitosan functional groups. Bioassays demonstrated

• the literature that larvicidal bioassays are effective tests for identifying new insecticides against this vector mosquito, as the larval stage is the longest and most vulnerable immature phase of the insect. In addition, this species is synanthropic, which means that it is adapted to live close to

• (WHO) for larvicidal bioassays, which applies the cumulative normal distribution model. In contrast, the bioassay conducted with the nanocomposite produced by the conventional ionic gelation method (CS/TPP–rot, Figure 5A) showed an LC50 of 157.02 ppm and an LC90 of 246.52 ppm, a value notably higher

Green synthesis of silver nanoparticles derived from algae and their larvicidal properties to control Aedes aegypti

• Matheus Alves Siqueira de Assunção,
• Douglas Dourado,
• Daiane Rodrigues dos Santos,
• Gabriel Bezerra Faierstein,
• Mara Elga Medeiros Braga,
• Severino Alves Junior,
• Rosângela Maria Rodrigues Barbosa,
• Herminio José Cipriano de Sousa and
• Fábio Rocha Formiga

Beilstein J. Nanotechnol. 2024, 15, 1566–1575, doi:10.3762/bjnano.15.123

• nanoparticles in controlling vector mosquitoes [62]. AgNPs synthesized from seaweed have been investigated as a vector control strategy based on their larvicidal properties. Table 2 summarizes data from bioassays with AgNPs synthesized from different species of seaweed against A. aegypti larvae. The mechanism

Development and characterization of potential larvicidal nanoemulsions against Aedes aegypti

• Jonatas L. Duarte,
• Leonardo Delello Di Filippo,
• Anna Eliza Maciel de Faria Mota Oliveira,
• Rafael Miguel Sábio,
• Gabriel Davi Marena,
• Tais Maria Bauab,
• Cristiane Duque,
• Vincent Corbel and
• Marlus Chorilli

Beilstein J. Nanotechnol. 2024, 15, 104–114, doi:10.3762/bjnano.15.10

• terpenes, as evidenced by the lower IC50 values of free terpenes compared to the nanoemulsions containing monoterpenes. The in vivo acute toxicity assessment in G. mellonella larvae indicated that the nanoemulsions exhibit a good toxicological profile. Finally, bioassays showed that terpene nanoemulsions

Plant growth regulation by seed coating with films of alginate and auxin-intercalated layered double hydroxides

• Vander A. de Castro,
• Valber G. O. Duarte,
• Danúbia A. C. Nobre,
• Geraldo H. Silva,
• Vera R. L. Constantino,
• Frederico G. Pinto,
• Willian R. Macedo and
• Jairo Tronto

Beilstein J. Nanotechnol. 2020, 11, 1082–1091, doi:10.3762/bjnano.11.93

• auxin 1-naphthalenoacetic acid (ZnAl-NAA-LDH), (ii) the coating of bean seeds (Phaseolus vulgaris L.) with composite films produced from mixtures of alginate polymer and ZnAl-NAA-LDH, and (iii) the evaluation of the plant response by bioassays. The hybrid ZnAl-NAA-LDH was characterized by a set of

• analytical techniques, including powder X-ray diffraction, thermogravimetric analysis coupled to differential scanning calorimetry and mass spectrometry, specific surface area measurement, and scanning electron microscopy. Bioassays were performed with the seeds coated with the composite film to assess the

• , fresh root matter and shoot length of plants. Thus, films produced from a mixture of alginate and the hybrid material containing the growth regulator intercalated into LDH can be a viable alternative to enhance plant development, which can be included in seed management. Keywords: bioassays

Small protein sequences can induce cellular uptake of complex nanohybrids

• Jan-Philip Merkl,
• Malak Safi,
• Christian Schmidtke,
• Fadi Aldeek,
• Johannes Ostermann,
• Tatiana Domitrovic,
• Sebastian Gärtner,
• John E. Johnson,
• Horst Weller and
• Hedi Mattoussi

Beilstein J. Nanotechnol. 2019, 10, 2477–2482, doi:10.3762/bjnano.10.238

• fluorescent, or magnetic and fluorescent nanoparticles have attracted much attention for the development of bioassays, their use as cellular labelling platforms has been less explored [2][3]. A few demonstrations describing the use of such hybrid nanostructures in cell labelling have been recently reported

Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina

• Marco Salerno,
• Amirreza Shayganpour,
• Barbara Salis and
• Silvia Dante

Beilstein J. Nanotechnol. 2017, 8, 74–81, doi:10.3762/bjnano.8.8

• confirmed on quartz-Au by QCM-D technique. The present results point to the possible future use of the tAPA–Au surfaces as disposable substrates for assessing the cell functionality in biosensors/bioassays. SEM images (20,000× magnification, scale bar 1 µm) of tAPA substrates (thickness ≈500 nm), a) as

NanoE-Tox: New and in-depth database concerning ecotoxicity of nanomaterials

• Katre Juganson,
• Angela Ivask,
• Irina Blinova,
• Monika Mortimer and
• Anne Kahru

Beilstein J. Nanotechnol. 2015, 6, 1788–1804, doi:10.3762/bjnano.6.183

• ethically questionable when bioassays with vertebrates are conducted. Therefore, alternative methods, e.g., models for predicting toxicity mechanisms of ENMs based on their physico-chemical properties (e.g., quantitative (nano)structure-activity relationships, QSARs/QNARs), should be developed. While the

Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• investigated with Human IL-8 HTRF® Assays (Cisbio Bioassays, Codolet, France) using the cell-supernatant protocol as recommended by the manufacturer. In brief, 0.5 × 106 cells were seeded in 12-well plates and incubated with 300 µg/mL nanoparticles for 24 h in basal medium. Afterwards, the cells were washed 3