Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

• Wicem Argoubi,
• Maroua Saadaoui,
• Sami Ben Aoun and
• Noureddine Raouafi

Beilstein J. Nanotechnol. 2015, 6, 1840–1852, doi:10.3762/bjnano.6.187

• , produced in goat), bovine serum albumin (98%), ascorbic acid, dopamine, H2O2, silica gel 60 mesh and F254 nm fluorescent silica-coated aluminum TLC plates were purchased from Sigma-Aldrich (Germany) and used as received without further purification. All solvents used were of analytical grade and were used

Probing fibronectin–antibody interactions using AFM force spectroscopy and lateral force microscopy

• Andrzej J. Kulik,
• Małgorzata Lekka,
• Kyumin Lee,
• Grazyna Pyka-Fościak and
• Wieslaw Nowak

Beilstein J. Nanotechnol. 2015, 6, 1164–1175, doi:10.3762/bjnano.6.118

• for a similar type of interaction between an antigen and an antibody (i.e., bovine serum albumin and its monoclonal antibody) [35]. Our experiments do not allow for determination of which region (module) of FN interacts with Mab. As the bond dissociation is a nonequilibrium, dynamic process

Influence of gold, silver and gold–silver alloy nanoparticles on germ cell function and embryo development

• Ulrike Taylor,
• Daniela Tiedemann,
• Christoph Rehbock,
• Wilfried A. Kues,
• Stephan Barcikowski and
• Detlef Rath

Beilstein J. Nanotechnol. 2015, 6, 651–664, doi:10.3762/bjnano.6.66

• ) and oligonucleotide conjugated AuNP (diameter 7.3 nm, 94 biomolecules per particle, Zeta potential −32 mV) which were tested by using bovine sperm, as well as bovine serum albumin (BSA) coated gold (diameter 6–20 nm), silver (diameter 11 nm; AgNP) and various gold silver alloy nanoparticles (silver

• concentration was 10 µg/mL and all particles were conjugated with bovine serum albumin. Reproduced with permission from [50]. Copyright 2014 Royal Society of Chemistry. Representative laser scanning microscope images of porcine cumulus–oocyte complexes after 46 h co-incubation during in vitro maturation. (A

• ) conjugated to bovine serum albumin (BSA) as measured by disc centrifugation. xc has been calculated by log-normal fitting. (B) Oocyte maturation rates after exposure to AgNP in situ or ex situ bioconjugated to BSA. *p < 0.05. Blastocyst development rates after microinjection of nanoparticles into 2-cell

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• . The silica-coated magnetic and fluorescent NPs were then conjugated with bovine serum albumin (BSA) by using N-hydroxysuccinimide (NHS) as cross linker. The synthesized NPs were then characterized by XRD, IR, TEM, absorption and fluorescence spectroscopy. The XRD diffraction peaks indicate the cubic

The fate of a designed protein corona on nanoparticles in vitro and in vivo

• Denise Bargheer,
• Julius Nielsen,
• Gabriella Gébel,
• Markus Heine,
• Sunhild C. Salmen,
• Roland Stauber,
• Horst Weller,
• Joerg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 36–46, doi:10.3762/bjnano.6.5

• nm) is much smaller than the adsorbent. If the nanoparticle and protein are in the same size, the Langmuir adsorption formalism seems not to be valid. This was discussed by Liu et al. using small CeO2-particles and bovine serum albumin with similar size of 7 nm [36]. The smaller surface free energy

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• the presence of the proteins bovine serum albumin (BSA) and casein the fluorescence intensities were discernibly decreased, no matter of which size the NPs were. We speculate that a protein coating produced by passive adsorption of the proteins to the NPs is responsible for this observed reduction in

Biopolymer colloids for controlling and templating inorganic synthesis

• Laura C. Preiss,
• Katharina Landfester and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2014, 5, 2129–2138, doi:10.3762/bjnano.5.222

• synthetic polymers (see Section 4 in [60] for a review), but only a limited number of works are found for biopolymers. Li et al. [61] prepared cross-linked chitosan microspheres and immobilized bovine serum albumin covalently on their surface. On the resulting particles, silica was formed by a sol–gel

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

Imaging the intracellular degradation of biodegradable polymer nanoparticles

• Anne-Kathrin Barthel,
• Martin Dass,
• Melanie Dröge,
• Jens-Michael Cramer,
• Daniela Baumann,
• Markus Urban,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2014, 5, 1905–1917, doi:10.3762/bjnano.5.201

• bovine serum albumin (BSA), which is a main component of FCS that is added to the cell medium. The results showed that BSA significantly increased the exocytosis of the tested PLGA nanoparticles [28]. Given that we used FCS in the cell medium, the high exocytosis might be responsible for the significant

In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics

• Wolfgang G. Kreyling,
• Stefanie Fertsch-Gapp,
• Martin Schäffler,
• Blair D. Johnston,
• Nadine Haberl,
• Christian Pfeiffer,
• Jörg Diendorf,
• Carsten Schleh,
• Stephanie Hirn,
• Manuela Semmler-Behnke,
• Matthias Epple and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180

• proteins. This plethora of corona proteins will mediate the NP navigation within body fluids and across membranes and provides a plausible interpretation of the very many sites of retention and accumulation of NP in various organs and tissues of the organism. Bovine serum albumin (BSA) depletion in the

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• ]. Biomolecules have been reported to interact with gold salts and reduce them into metallic gold, acting both as a reductant and stabilizer [12][17][18][19][20][21]. Proteins, such as bovine serum albumin, silk fibroin protein, chicken egg white lysozyme, α-amylase, green fluorescent protein and apoferritin have

• mg/mL and 0.5 mM, respectively. These conditions did not result in the formation of AuNPs. The addition of Ag ions to a mixture containing Au ions has been reported to form AuNPs in the presence of bovine serum albumin (BSA) [33]. Hence, we added AgNO3 at a ratio of 5:1 between the Au and Ag ions and

• histone (HIS), chicken egg white lysozyme (LYS), ovalbumin (OVA), bovine serum albumin (BSA), bovine hemoglobin (BHG), bovine gamma globulin (BGG), glucose oxidase (Aspergillus niger) (GOX), and trypsin (porcine pancreas, TRY), used for the experiments were obtained from Sigma (USA) as a lyophilized

Antimicrobial nanospheres thin coatings prepared by advanced pulsed laser technique

• Alina Maria Holban,
• Valentina Grumezescu,
• Alexandru Mihai Grumezescu,
• Bogdan Ştefan Vasile,
• Roxana Truşcă,
• Rodica Cristescu,
• Gabriel Socol and
• Florin Iordache

Beilstein J. Nanotechnol. 2014, 5, 872–880, doi:10.3762/bjnano.5.99

• polymeric microspheres. Thus, Socol et al., [43], firstly reported the novel deposition of PLGA–PVA, PLGA–PVA–BSA (bovine serum albumin) and PLGA–PVA–CS microspheres by matrix assisted pulsed laser evaporation (MAPLE) technique. SEM images of thin coatings reveal homogeneous and spherical-shaped particles

• in the micrometric range. The average diameter of PLGA–PVA, PLGA–PVA–BSA (bovine serum albumin) and PLGA–PVA–CS particles ranged from 180 to 250 nm. Grumezescu et al., [34], reported the MAPLE fabrication of PLA–PVA–UA microsphere thin coatings. These thin coatings possessed a homogeneous shape and

Porous polymer coatings as substrates for the formation of high-fidelity micropatterns by quill-like pens

• Michael Hirtz,
• Marcus Lyon,
• Wenqian Feng,
• Andrea E. Holmes,
• Harald Fuchs and
• Pavel A. Levkin

Beilstein J. Nanotechnol. 2013, 4, 377–384, doi:10.3762/bjnano.4.44

• micrometres. The bromophenol blue arrays on HEMA support were used to detect the presence of bovine serum albumin (BSA). In the presence of BSA, the fluorescence spectrum observed from the bromophenol blue microarray exhibited a significant red shift of the maximum emission wavelength. Our results show that

• samples [12][13]. After preparing 10 × 10 spot microarrays using bromophenol blue solution as shown in the inset of Figure 6b, the patterns were either incubated with a 0.5 µL droplet of an analyte solution containing bovine serum albumin (BSA) or a clear solution to give a negative control. Fluorescence

Macromolecular shape and interactions in layer-by-layer assemblies within cylindrical nanopores

• Thomas D. Lazzara,
• K. H. Aaron Lau,
• Wolfgang Knoll,
• Andreas Janshoff and
• Claudia Steinem

Beilstein J. Nanotechnol. 2012, 3, 475–484, doi:10.3762/bjnano.3.54

• molecular recognition of biotinylated-bovine serum albumin (b-BSA) by avidin. Avidin has four biotin-binding sites, whereas the b-BSA used has 13 biotin molecules per protein on average. Avidin with a mass of MW = 66–69 kDa, and which is positively charged at neutral pH, was first adsorbed onto the

• Calbiochem (purity 12.9 units/mg). Biotinylated-bovine serum albumin (b-BSA) with 13 mol biotin/mol albumin, poly(sodium 4-styrenesulfonate) (PSS) (Mw = 70 kDa), poly(allylamine hydrochloride) PAH (Mw = 50–65 kDa), CuCl2, NaCl, and 16-mercaptohexadecanoic acid (90%) were purchased from Sigma Aldrich (St

• serum albumin was compared with that of linear polyelectrolytes (linear-PEs), both species being of similar molecular weight. LbL deposition within the cylindrical AAO geometry for different pore diameters (d0 = 25–80 nm) for the various macromolecular species, showed that the multilayer film growth was

Analysis of fluid flow around a beating artificial cilium

• Mojca Vilfan,
• Gašper Kokot,
• Andrej Vilfan,
• Natan Osterman,
• Blaž Kavčič,
• Igor Poberaj and
• Dušan Babič

Beilstein J. Nanotechnol. 2012, 3, 163–171, doi:10.3762/bjnano.3.16

• diameter 55 nm [24]) in water. To prevent aggregation of the beads, we coated them with BSA (bovine serum albumin), 10 mg/mL, for 4 h in an ultrasonic bath. One end of the assembled chain was attached to the surface through prefabricated ferromagnetic-nickel anchoring sites. The nickel dots were

Magnetic nanoparticles for biomedical NMR-based diagnostics

• Huilin Shao,
• Tae-Jong Yoon,
• Monty Liong,
• Ralph Weissleder and
• Hakho Lee

Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17

• bovine serum albumin (BSA) protein as a control did not elicit any change in T2 (Figure 5b). More recently, MRSw biosensors, capable of detecting soluble tumor biomarker proteins (such as CA-125, VEGF, and α-fetoprotein) were described, and used for parallel detection of multiple markers in blood samples