The cell-type specific uptake of polymer-coated or micelle-embedded QDs and SPIOs does not provoke an acute pro-inflammatory response in the liver

• Markus Heine,
• Alexander Bartelt,
• Oliver T. Bruns,
• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Ludger Scheja,
• Christian Waurisch,
• Alexander Eychmüller,
• Rudolph Reimer,
• Horst Weller,
• Peter Nielsen and
• Joerg Heeren

Beilstein J. Nanotechnol. 2014, 5, 1432–1440, doi:10.3762/bjnano.5.155

• employing various cell culture systems described toxic effects of QDs [3][4]. Iron-containing superparamagnetic iron oxide nanocrystals (SPIOs) used for magnetic resonance imaging (MRI) have a relative good reputation given that iron is an essential trace element and it can be assumed that iron from

• degraded SPIOs is transferred to the body iron stores. Nevertheless, iron-induced acute toxic reactions, probably related to the generation of reactive oxygen species, have been described in vitro after uptake of large amount of various SPIOs [5]. However, cell culture studies like the ones described above

Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii

• Sören B. Gutekunst,
• Carsten Grabosch,
• Alexander Kovalev,
• Stanislav N. Gorb and
• Christine Selhuber-Unkel

Beilstein J. Nanotechnol. 2014, 5, 1393–1398, doi:10.3762/bjnano.5.152

• culture flasks in order to avoid an encystment of A. castellanii trophozoites. Adhesion experiments The PDMS substrates were washed with PYG 712 medium before use. A. castellanii were detached from the cell culture substrate by cautiously hitting the culture flask. The acanthamoebae were counted by a

• (SO4)2·6H2O (AppliChem, Darmstadt, Germany), 10.0 mL 0.25 M Na2HPO4·7H2O (Roth, Karlsruhe, Germany), 10.0 mL 0.25 M KH2PO4 (Roth, Karlsruhe, Germany), 50.0 mL 2 M glucose (Sigma–Aldrich Chemie GmbH, Steinheim, Germany)). In this axenic culture, the PYG 712 medium was regularly exchanged in the cell

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

• Dominic Docter,
• Christoph Bantz,
• Dana Westmeier,
• Hajo J. Galla,
• Qiangbin Wang,
• James C. Kirkpatrick,
• Peter Nielsen,
• Michael Maskos and
• Roland H. Stauber

Beilstein J. Nanotechnol. 2014, 5, 1380–1392, doi:10.3762/bjnano.5.151

• methods. First, the size, spherical shape and homogeneity of the ASP were visualized by transmission electron microscopy (TEM) (Figure 1). Next, we examined the stability of the ASP dispersions in water, salt-containing buffer (buffer A), and cell culture medium (DMEM) with or without the addition of 10

• cell culture medium. Hence, the tested concentrations of ions, carbohydrates and supplements, such as vitamins, did not significantly affect the ASP profiles. Albeit other (bio)molecules besides proteins are known or at least discussed to adsorb to NP [7][40], their impact on the physico-chemical

• (103.5 mM NaCl, 5.3 mM KCl, 5.6 mM Na2HPO4, 1.4 mM KH2PO4, 23.8 mM NaHCO3, pH 7.4), DMEM with or without 10% FCS, and the measurements were conducted at 25 °C by using 0.6 mg/mL ASP. Cell culture The colonic carcinoma cell line Caco-2 and the colorectal adenocarcinoma cell line HT-29 were obtained from

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• inside the cells or attached to the cell surface. Due to the cultivation of the cells on cell culture inserts, the pores of the insert membranes become visible. Compared to unexposed cells, large NP aggregates can be seen as dark spots in Ag NP-treated cell cultures. TEM was used to resolve the Ag NPs

• with 2.7 ± 1.3 fold (p = 0.056) relative to the negative control. LDH levels for cells exposed under submerged conditions were determined in the upper compartment of the transwell insert as well as in the lower compartment (Figure 4B). Due to differences in the amount of cell culture medium used in the

• (data not shown). Cytokine/chemokine secretion As described in [44], the release of the pro-inflammatory markers TNF-α and IL-8 was measured 4 and 24 h after exposure by enzyme linked immunosorbent assay (ELISA) to characterize the pro-inflammatory response of the cell culture lung model (Figure 5

PEGylated versus non-PEGylated magnetic nanoparticles as camptothecin delivery system

• Paula M. Castillo,
• Mario de la Mata,
• Maria F. Casula,
• José A. Sánchez-Alcázar and
• Ana P. Zaderenko

Beilstein J. Nanotechnol. 2014, 5, 1312–1319, doi:10.3762/bjnano.5.144

• cell culture loaded with: CPT (left); USM[CPT] formulation (middle); USM-PEG[CPT] formulation (right) and details of apoptotic nuclei (inserted). C: Percentage of apoptotic cell nuclei in H460 cell cultures: From left to right, control, with bare USM, with PEGylated USM, with CPT, with USM[CPT], USM

Nanocavity crossbar arrays for parallel electrochemical sensing on a chip

• Enno Kätelhön,
• Dirk Mayer,
• Marko Banzet,
• Andreas Offenhäusser and
• Bernhard Wolfrum

Beilstein J. Nanotechnol. 2014, 5, 1137–1143, doi:10.3762/bjnano.5.124

• operated in a parallel readout for high-throughput applications. The redox cycling response during electrochemical imaging using parallel data acquisition is demonstrated and different modes of operation for its future use in mapping neurochemical events in cell culture are discussed. Results and

Nanodiamond-DGEA peptide conjugates for enhanced delivery of doxorubicin to prostate cancer

• Amanee D Salaam,
• Patrick Hwang,
• Roberus McIntosh,
• Hadiyah N Green,
• Ho-Wook Jun and
• Derrick Dean

Beilstein J. Nanotechnol. 2014, 5, 937–945, doi:10.3762/bjnano.5.107

• ) was used to measure the zeta potential and hydrodynamic size of the ND before and after modification with DGEA and DOX; samples were prepared at a concentration of 200 µg/mL. Cell culture Human bone metastatic prostate cancer cells (PC3) and mesenchymal stem cells (hMSC) were acquired from American

Antimicrobial properties of CuO nanorods and multi-armed nanoparticles against B. anthracis vegetative cells and endospores

• Pratibha Pandey,
• Merwyn S. Packiyaraj,
• Himangini Nigam,
• Gauri S. Agarwal,
• Beer Singh and
• Manoj K. Patra

Beilstein J. Nanotechnol. 2014, 5, 789–800, doi:10.3762/bjnano.5.91

• fraction in B. anthracis vegetative cell culture in most of the batches with the exception of few. The nanoparticles probably kill the cells by damaging the cell membrane mechanically, leading to leakage of cellular content. SEM observations discussed in later paragraphs support this probability. The broad

• micrographs of a) B. anthracis control cells at 5000× the arrows show occasional spores population present in the cell culture. b) B. anthracis control spores formed in G-media after seven days of incubation at 10000× magnification. Antibacterial activity of multi-armed copper oxide NPs (P5) against B

In vitro toxicity and bioimaging studies of gold nanorods formulations coated with biofunctional thiol-PEG molecules and Pluronic block copolymers

• Tianxun Gong,
• Douglas Goh,
• Malini Olivo and
• Ken-Tye Yong

Beilstein J. Nanotechnol. 2014, 5, 546–553, doi:10.3762/bjnano.5.64

• in water. Concentrations of the AuNRs solutions were fixed at an optical density of 1.5 for our studies. Cell culture and cell viability: As described in [27], oral squamous cell carcinoma (OSCC) cell line was cultured in DMEM containing 10% FBS with Pen Strep. All cultures were kept at 37 °C with 5

Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating

• Tingjun Lei,
• Alicia Fernandez-Fernandez,
• Romila Manchanda,
• Yen-Chih Huang and
• Anthony J. McGoron

Beilstein J. Nanotechnol. 2014, 5, 313–322, doi:10.3762/bjnano.5.35

• IR820 can be used in lieu of ICG in imaging and hyperthermia applications. Three-minute laser exposure (power at 1440 J/cm2) with 5 µM IR820 or ICG can elevate the temperature of cell culture media from 37 °C to 42 °C or from 37 °C to 46 °C, respectively [9]. Despite the fact that IR820 has a lower

• by using a cell culture incubator. The results indicated that the promotion of HSP70 was minimized during rapid heating. As mentioned before, ROS can activate the expression of HSP70. The inhibition of HSP70 during rapid-rate and low thermal dose heating could possibly mean the abolishment of ROS

• was evaluated by using a Cary WinUV spectrophotometer (Varian/Agilent Technologies, Switzerland). In vitro studies of NPs Cancer cells MES-SA, Dx5, and SKOV3 were purchased from American Type Culture Collection (Manassas, VA) along with McCoy’s 5A medium and fetal bovine serum. Cell culture supplies

FTIR nanobiosensors for Escherichia coli detection

• Stefania Mura,
• Gianfranco Greppi,
• Maria Laura Marongiu,
• Pier Paolo Roggero,
• Sandeep P. Ravindranath,
• Lisa J. Mauer,
• Nicoletta Schibeci,
• Francesco Perria,
• Massimo Piccinini,
• Plinio Innocenzi and
• Joseph Irudayaraj

Beilstein J. Nanotechnol. 2012, 3, 485–492, doi:10.3762/bjnano.3.55

• were used without further purification. Titanium tetrachloride (TiCl4, >98%), anhydrous ethanol (EtOH, >99.9%), bidistilled water, acetone (>99.8%), and toluene (>99.5%), were purchased from Carlo Erba (Italy). Pluronic F-127 (cell culture test), (3-aminopropyl)triethoxysilane (APTES, >98

Magnetic-Fe/Fe₃O₄-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages

• Hongwang Wang,
• Tej B. Shrestha,
• Matthew T. Basel,
• Raj K. Dani,
• Gwi-Moon Seo,
• Sivasai Balivada,
• Marla M. Pyle,
• Heidy Prock,
• Olga B. Koper,
• Prem S. Thapa,
• David Moore,
• Ping Li,
• Viktor Chikan,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2012, 3, 444–455, doi:10.3762/bjnano.3.51

• . To measure the temperature change, a fiber optic probe (Neoptix, Quebec, Canada) was used. Cell culture RAW264.7 cells (mouse monocyte/macrophage-like cells, Mo/Ma) were cloned with the rabbit carboxylesterase (InCE) gene with Tet-On system and made double stable. Generation of the double-stable

Fabrication of multi-parametric platforms based on nanocone arrays for determination of cellular response

• Lindarti Purwaningsih,
• Tobias Schoen,
• Tobias Wolfram,
• Claudia Pacholski and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2011, 2, 545–551, doi:10.3762/bjnano.2.58

• with gold tips can later be coated with different molecules such as proteins or antibodies. Cell culture and cell adhesion experiments: Cell culture maintenance for SHSY-5Y neuroblastoma cells was performed as described previously [24]. The substrates for cell adhesion experiments were passivated with