PLGA nanoparticles as a platform for vitamin D-based cancer therapy

• Maria J. Ramalho,
• Joana A. Loureiro,
• Bárbara Gomes,
• Manuela F. Frasco,
• Manuel A. N. Coelho and
• M. Carmo Pereira

Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135

• cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 μM for the S2-013 cells (91%) and for A549 cells (70%) comparared

• cell growth, cell cycle arrest and morphological changes. Results Nanoparticle physicochemical properties PLGA NPs were prepared by a single emulsion solvent evaporation method and stabilized with Pluronic®F127. The obtained results for mean the diameter, polydispersity index (PDI) and zeta potential

• morphological features are consistent with senescence phenomena. Cell growth inhibition by calcitriol-loaded NPs The in vitro cytotoxic effects on three different human cell lines, hTERT-HPNE, S2-013 and A549, after treatment with calcitriol entrapped into the PLGA NPs were assessed relative to free calcitriol

Novel ZnO:Ag nanocomposites induce significant oxidative stress in human fibroblast malignant melanoma (Ht144) cells

• Syeda Arooj,
• Samina Nazir,
• Akhtar Nadhman,
• Nafees Ahmad,
• Bakhtiar Muhammad,
• Ishaq Ahmad,
• Kehkashan Mazhar and
• Rashda Abbasi

Beilstein J. Nanotechnol. 2015, 6, 570–582, doi:10.3762/bjnano.6.59

• generated and their IC50 values were calculated. The IC50 value for the nanoparticles represents the concentration that inhibits 50% of cell growth. The experiments were performed twice with triplicates for each sample. Detection of singlet oxygen by chemical trapping 1,3-Diphenylisobenzofuran (DPBF) was

Filling of carbon nanotubes and nanofibres

• Reece D. Gately and
• Marc in het Panhuis

Beilstein J. Nanotechnol. 2015, 6, 508–516, doi:10.3762/bjnano.6.53

• developing a scalable method for producing larger quantities of nanowires has been undertaken. As TCNSs have shown promise in the field of tissue engineering, with further development, this method may be used to produce channels for cell growth. Filled with appropriate drugs and medium, TCNSs may provide a

Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants

• Anna Maria Pappa,
• Varvara Karagkiozaki,
• Silke Krol,
• Spyros Kassavetis,
• Dimitris Konstantinou,
• Charalampos Pitsalidis,
• Lazaros Tzounis,
• Nikos Pliatsikas and
• Stergios Logothetidis

Beilstein J. Nanotechnol. 2015, 6, 254–262, doi:10.3762/bjnano.6.24

• and its effect on cell attachment and growth was evaluated. The conditions of the surface modification were properly adjusted in order to define those conditions of the treatment that result in surfaces favorable for cell growth, while maintaining morphological integrity and mechanical behavior

• , Germany) was used to evaluate cell viability. The cells used in this study were mouse fibroblasts L929 and were kindly offered from the Department of Biochemistry of the Aristotle University of Thessaloniki. In a similar manner as described in [32], the cell growth was stopped after 1 day, 3 days and 1

Functionalization of α-synuclein fibrils

• Simona Povilonienė,
• Vida Časaitė,
• Virginijus Bukauskas,
• Arūnas Šetkus,
• Juozas Staniulis and
• Rolandas Meškys

Beilstein J. Nanotechnol. 2015, 6, 124–133, doi:10.3762/bjnano.6.12

• containing ampicillin (50 μg/mL). The cells were grown at 37 °C until the OD600 reached 0.8; the protein expression was induced by the addition of 0.2 mM IPTG, and cell growth continued at 30 °C for 18 h. The biomass was collected by centrifugation at 4000g and resuspended in 50 mM Tris-HCl buffer (pH 8.0

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• Planck Institute for Biology of Ageing, Joseph-Stelzmann-Straße 9b, 50931 Cologne, Germany 10.3762/bjnano.5.257 Abstract In this work, we study epithelial cell growth on substrates decorated with gold nanorods that are functionalized either with a positively charged cytotoxic surfactant or with a

• biocompatible polymer exhibiting one of two different end groups, resulting in a neutral or negative surface charge of the particle. Upon observation of cell growth for three days by live cell imaging using optical dark field microscopy, it was found that all particles supported cell adhesion while no directed

• cell migration and no significant particle internalization occurred. Concerning cell adhesion and spreading as compared to cell growth on bare substrates after 3 days of incubation, a reduction by 45% and 95%, respectively, for the surfactant particle coating was observed, whereas the amino-terminated

Functionalized polystyrene nanoparticles as a platform for studying bio–nano interactions

• Cornelia Loos,
• Tatiana Syrovets,
• Anna Musyanovych,
• Volker Mailänder,
• Katharina Landfester,
• G. Ulrich Nienhaus and
• Thomas Simmet

Beilstein J. Nanotechnol. 2014, 5, 2403–2412, doi:10.3762/bjnano.5.250

• effects on cell growth [4]. Nanoparticles Nanoparticles, limited in size to 100 nm in either two or three dimensions [18], fill the gap between molecules and bulk material and between biomolecules and cells. The atoms located at the surface of a nanoparticle have less neighbors than atoms of a bulk

• rapamycin (mTOR), a key kinase controlling cell growth and proliferation and implicated in many human diseases including cancer and diabetes [65]. Thus, the integrity of membranes of acidic lysosomal compartments are important for the activation of mTOR [65]. We could show that PS-NH2 inhibits, whereas PS

Effect of silver nanoparticles on human mesenchymal stem cell differentiation

• Christina Sengstock,
• Jörg Diendorf,
• Matthias Epple,
• Thomas A. Schildhauer and
• Manfred Köller

Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214

• phosphatase [50]. Metal ions are generally assumed to have a significant effect on the activation of redox-sensitive transcription factors such as NF-κB or AP-1 [51]. These transcription factors are involved in inflammatory responses and are important for processes such as differentiation and cell growth [52

Carbon-based smart nanomaterials in biomedicine and neuroengineering

• Antonina M. Monaco and
• Michele Giugliano

Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196

• substrates for cell growth, by virtue of their peerless optical, electrical, thermal, and mechanical properties. In this review we discuss the state-of-the-art in the applications of nanomaterials in biological and biomedical fields, with a particular emphasis on neuroengineering. Keywords: carbon nanotubes

• -regulated for hNSCs grown on graphene. Akhavan et al. [145] studied the differentiation of hNSCs on GO nanogrids deposited on a substrate made of TiO2 nanoparticles over SiO2, which made them photosensitive. The authors observed an increase in cell growth and alignment along the geometrical pattern of the

Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells

• Claudia Strobel,
• Martin Förster and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2014, 5, 1795–1807, doi:10.3762/bjnano.5.190

• Chemie GmbH, Germany)); or endothelial cell growth medium (Ready-to-use, PromoCell GmbH, Germany; supplemented with SupplementMix, PromoCell GmbH, Germany; FBS 2% (v/v))). Cell culture experiments The experiments were performed with immortalized human microvascular endothelial cells (HMEC-1; Centers for

• , Germany), GlutaMAXTM I 100X (1% (v/v), Life Technologies GmbH, Germany), hydrocortisone (1 µg/mL; Sigma-Aldrich Chemie GmbH, Germany) and epidermal growth factor (10 ng/mL; Life Technologies GmbH, Germany). HUVEC were cultivated in endothelial cell growth medium (Ready-to-use, PromoCell GmbH, Germany

Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy

• Adriano A. Torrano and
• Christoph Bräuchle

Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173

• 1.1 × 104 cells·cm−2. HeLa and HUVEC cells were incubated with silica nanoparticles as described before [4]. HMEC-1 cells were incubated with ceria nanoparticles in humidified 5% CO2 atmosphere at 37 °C. The 10 µg·mL−1 solution of ceria nanoparticles was prepared in the same cell medium used for cell

• growth. Before addition to cells, the solution was vortexed for 10 s, treated in an ultrasonic bath for 10 min and vortexed again for 10 s. After the incubation time, and just before measurements, the cell membrane was stained with a solution of 10 µg·mL−1 wheat germ agglutinin, Alexa Fluor® 488 (Life

Growth and structural discrimination of cortical neurons on randomly oriented and vertically aligned dense carbon nanotube networks

• Christoph Nick,
• Sandeep Yadav,
• Ravi Joshi,
• Christiane Thielemann and
• Jörg J. Schneider

Beilstein J. Nanotechnol. 2014, 5, 1575–1579, doi:10.3762/bjnano.5.169

• poly-D-lysine) prior to cell cultivation was performed. The cell growth behaviour in vitro was studied by scanning electron microscopy (SEM) on pristine vertically aligned CNT arrays and compared to growth of cortical neurons on islands of randomly oriented CNTs under the same conditions. The cells had

• reported ability to tailor the hydrophilicity and hydrophobicity of such 3D aligned CNT structures over a wide range from superhydrophilic to superhydrophobic [29] the directional cell growth on such structures should be possible and would thus allow understanding these observed preferences from a surface

• chemistry viewpoint in future work. In conclusion, pristine randomly and vertically aligned CNTs architectures were studied with respect their use as substrates for neuron cell growth. Both CNT architectures are unique hierarchical structures to direct and control neural cell growth. To the best of our

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

• Christoph Rehbock,
• Jurij Jakobi,
• Lisa Gamrad,
• Selina van der Meer,
• Daniela Tiedemann,
• Ulrike Taylor,
• Wilfried Kues,
• Detlef Rath and
• Stephan Barcikowski

Beilstein J. Nanotechnol. 2014, 5, 1523–1541, doi:10.3762/bjnano.5.165

• nanoscopic wear debris [6][7][8] which have been reported to accumulate in lymph nodes, bone marrow, liver and spleen [9]. In that context toxicological effects, including impaired DNA replication and cell growth as well as inflammatory responses, are meant to originate from release of toxic heavy metal ions

The softening of human bladder cancer cells happens at an early stage of the malignancy process

• Jorge R. Ramos,
• Joanna Pabijan,
• Ricardo Garcia and
• Malgorzata Lekka

Beilstein J. Nanotechnol. 2014, 5, 447–457, doi:10.3762/bjnano.5.52

• , cancer progression changes its role by using it as a tool to alter cell growth, stiffness, movement and invasiveness [26]. The actin cytoskeleton serves as a scaffold for signaling, as a connection to the extracellular environment, and as a mechanosensor. However, there is no general evidence that

Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating

• Tingjun Lei,
• Alicia Fernandez-Fernandez,
• Romila Manchanda,
• Yen-Chih Huang and
• Anthony J. McGoron

Beilstein J. Nanotechnol. 2014, 5, 313–322, doi:10.3762/bjnano.5.35

• significantly impact cell growth. It is also noteworthy that NP concentrations equivalent to 5 µM IR820 had a slight cell growth inhibition effect on MES-SA cells. This is in line with our previous observations on the cytotoxicity effects of free IR820 on MES-SA, and seems to be related to the fact that drug

• publications [24][42]. The effect of each treatment on cell growth was normalized to the growth of the control group, which did not receive any treatment. Cell-based assays for the detection of ROS, HIF-1 and VEGF expression Study of ROS expression Intracellular ROS level was measured by using the fluorescent

Growth behaviour and mechanical properties of PLL/HA multilayer films studied by AFM

• Cagri Üzüm,
• Johannes Hellwig,
• Narayanan Madaboosi,
• Dmitry Volodkin and
• Regine von Klitzing

Beilstein J. Nanotechnol. 2012, 3, 778–788, doi:10.3762/bjnano.3.87

• ]. Engler et al. reported a similar spreading behaviour for smooth-muscle cells [3]. An enhanced cell growth was observed also for cross-linked chitosan/hyaluronan multilayer films, as compared to the native ones [8]. These reports suggest that polyelectrolyte multilayer films are ideal matrices for bio

Colloidal lithography for fabricating patterned polymer-brush microstructures

• Tao Chen,
• Debby P. Chang,
• Rainer Jordan and
• Stefan Zauscher

Beilstein J. Nanotechnol. 2012, 3, 397–403, doi:10.3762/bjnano.3.46

• brushes [13] are of increasing importance especially for array-based platforms because of their ability to modify surface properties and their potential applications in surface-based technologies, such as protein-resistant coatings, switchable sensors, substrates for cell-growth control, and for the

• array-based platforms and applications in surface-based technologies, such as protein-resistant coatings, switchable sensors, substrates for cell-growth control, and for separation of biological molecules. We note that for convenience and proof-of-concept of our approach, we used PS microspheres to

Approaches to nanostructure control and functionalizations of polymer@silica hybrid nanograss generated by biomimetic silica mineralization on a self-assembled polyamine layer

• Jian-Jun Yuan and
• Ren-Hua Jin

Beilstein J. Nanotechnol. 2011, 2, 760–773, doi:10.3762/bjnano.2.84

• , nanostructured thin films on substrates with tunable nanostructure and surface morphology are of great importance for various applications, such as photoelectronics [1], high-efficiency sensing and bioanalysis [2][3], protein adsorption [4], cell growth [5], surface wettability control [6] and liquid