Beilstein J. Nanotechnol.2011,2, 374–383, doi:10.3762/bjnano.2.43
scale of diffusion, τD. Based on the well-known spatial extension of the observation volume, the diffusioncoefficient, D, and, by using the Stokes–Einstein equation (see Experimental), the hydrodynamic radius of the fluorescent particle, RH, can be calculated. Consequently, a NP size increase due to
experiments and accounted for in the determination of the diffusioncoefficient, D, according to the Stokes–Einstein relation,
with hydrodynamic radius RH, Boltzmann constant kB, temperature T, and viscosity η. Three independent series of measurements were taken and averaged.
Data analysis
In conventional FCS
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Figure 1:
Fluorescence intensity correlation curves of NPs dissolved in buffer solutions of (a, b) HSA, (c, d...