Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

• Konstantin Paliienko,
• Artem Pastukhov,
• Michal Babič,
• Daniel Horák,
• Olga Vasylchenko and
• Tatiana Borisova

Beilstein J. Nanotechnol. 2020, 11, 1381–1393, doi:10.3762/bjnano.11.122

• on the Care and Use of Laboratory Animals 86/609/EEC) and the experimental protocols were approved by the Animal Care and Use Committee of the Palladin Institute of Biochemistry (Protocol from 19/09-2016). Ten animals were used in experiments analyzing ʟ-[14C]glutamate uptake, its extracellular level

• µg/mL changed the fluorescence intensity of rhodamine 6G, which was associated with a decrease in transparency of the analyzed solution. In the fluorescence experiments, a γ-Fe2O3/synaptosomal protein ratio similar to that used in the experiments regarding uptake and extracellular level of ʟ-[14C

• in uptake and an increase in the extracellular level of glutamate in nerve cells. It should be noted that different concentrations of γ-Fe2O3 nanoparticles were used in the experiments. Nanoparticles within a concentration range of 0.75–3 mg/mL were applied in ʟ-[14C]glutamate adsorption experiments

Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe₂O₃ nano-sized particles and assessment of their effects on glutamate transport

• Tatiana Borisova,
• Natalia Krisanova,
• Arsenii Borуsov,
• Roman Sivko,
• Ludmila Ostapchenko,
• Michal Babic and
• Daniel Horak

Beilstein J. Nanotechnol. 2014, 5, 778–788, doi:10.3762/bjnano.5.90

• characteristics of the glutamatergic neurotransmission were analysed. Using radiolabeled L-[14C]glutamate, it was shown that D-mannose-coated γ-Fe2O3 nanoparticles did not affect high-affinity Na+-dependent uptake, tonic release and the extracellular level of L-[14C]glutamate in isolated rat brain nerve terminals

• field using D-mannose-coated γ-Fe2O3 nanoparticles, while the key characteristics of glutamatergic neurotransmission are not affected. In other words, functionally active synaptosomes labeled with D-mannose-coated γ-Fe2O3 nanoparticles were obtained. Keywords: extracellular level; γ-Fe2O3; glutamate

• normal brain functioning. Impaired glutamate homeostasis causes neuronal dysfunction and contributes to the pathogenesis of major neurological disorders. For normal brain functioning, a low extracellular level of glutamate should be maintained between episodes of exocytotic release, thereby preventing