Beilstein J. Nanotechnol.2021,12, 1–23, doi:10.3762/bjnano.12.1
oligomerisation of the 33-mer peptide gliadin [74]. The HIM data helped the authors to “show a plausible pathway of 33-mer peptide protofilaments formation” via the contact of square-like oligomers and the formation of protofilaments by “longitutinal association of matured rod-like oligomers.”
Imaging animals and
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Figure 1:
Annual (bars) and accumulated (blue line) numbers of publications on bio-imaging using the helium-i...
Beilstein J. Nanotechnol.2017,8, 244–253, doi:10.3762/bjnano.8.27
(adsorption, directional, covalent and a combination thereof). As a result, AuNP probes prepared by direct adsorption were the most effective method. AuNPs probes were then used to detect gliadin, one of the main components of wheat gluten, the protein composite that causes celiac disease. With this optimized
approach, our data showed a sensitivity increase of at least five times and a lower detection limit with respect to a standard ELISA of at least three times. Additionally, the assay time was remarkably decreased.
Keywords: allergen; ELISA enhancement; functionalization; gliadin; gold nanoparticle
, the strategy which demonstrated better sensitivity was used for detection of gliadin from wheat gluten, one of the main proteins of wheat gluten [24]. Gluten refers to a group of proteins contained in wheat, barley and rye and is thought to be the cause of celiac disease (CD). CD is an autoimmune
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Figure 1:
Schematic representation of the four different functionalization methods explored in this work. (a)...