Beilstein J. Nanotechnol.2019,10, 967–974, doi:10.3762/bjnano.10.97
exponential decrease of the signal as it goes into the cladding. This means that biorecognition events must occur as close to the PBG structure surface as possible in order to obtain the maximum sensing response. Within this context, the PBG biosensor has been biofunctionalized with half-antibodies specific
to bovine serum albumin (BSA) using a UV-induced immobilization procedure. The use of half-antibodies allows one to reduce the thickness of the biorecognition volume down to ca. 2.5 nm, thus leading to a higher interaction with the evanescent field, as well as a proper orientation of their binding
sites towards the target sample. Then, the biofunctionalized PBG biosensor has been used to perform a direct and real-time detection of the target BSA antigen.
Keywords: evanescent field; half-antibodies; light-assisted immobilization; photonic bandgap sensor; SNOM characterization; Introduction
The
PDF
Figure 1:
(a) Optical microscopy image of the fabricated photonic chip. Each of the PBG sensors groups are ma...