Study of the reusability and stability of nylon nanofibres as an antibody immobilisation surface

• Inés Peraile,
• Matilde Gil-García,
• Laura González-López,
• Nushin A. Dabbagh-Escalante,
• Juan C. Cabria-Ramos and
• Paloma Lorenzo-Lozano

Beilstein J. Nanotechnol. 2024, 15, 83–94, doi:10.3762/bjnano.15.8

• need to reduce the risk of pandemic contamination. Early, reliable, and accurate diagnosis is therefore essential for health and food safety [4][5]. In this context, immunodetection seems to be a very good option [6]. There are many applications of immunoassay devices in health, food industry, and

• clinical applications. Immunoassay devices have been used not only for the detection of bacteria and viruses [7], but also for the measurement of drugs [8] and hormones [9], or for the determination of glucose in urine [10]. The specificity of antigen–antibody binding and how the antibody is attached to

Metal-organic framework-based nanomaterials as opto-electrochemical sensors for the detection of antibiotics and hormones: A review

• Akeem Adeyemi Oladipo,
• Saba Derakhshan Oskouei and
• Mustafa Gazi

Beilstein J. Nanotechnol. 2023, 14, 631–673, doi:10.3762/bjnano.14.52

• , persistence, ecological, and health risks associated with them. However, to detect these emerging contaminants, analytical techniques that are sensitive and selective enough must be developed due to their incredibly low concentrations [2][6][7][8][9]. Colourimetry, chromatography, enzyme-linked immunoassay

• , multistep separation procedure. A fluorescence polarisation immunoassay, which does not require a separation step, is one development that has been made to get around this restriction [22]. Furthermore, chromatographic techniques such as thin-layer chromatography (TLC), liquid chromatography-coupled mass

Studies of probe tip materials by atomic force microscopy: a review

• Ke Xu and
• Yuzhe Liu

Beilstein J. Nanotechnol. 2022, 13, 1256–1267, doi:10.3762/bjnano.13.104

• colloidal gold probes, and subjected to an immunoassay. The activity of the colloidal gold probe was determined by immunofiltration. The results showed that the colloidal gold probe was highly specific when applied to the immunodiafiltration method, and positive samples could be determined by direct color

Selective detection of complex gas mixtures using point contacts: concept, method and tools

• Alexander P. Pospelov,
• Victor I. Belan,
• Dmytro O. Harbuz,
• Volodymyr L. Vakula,
• Lyudmila V. Kamarchuk,
• Yuliya V. Volkova and
• Gennadii V. Kamarchuk

Beilstein J. Nanotechnol. 2020, 11, 1631–1643, doi:10.3762/bjnano.11.146

• contributes to the development of new noninvasive tests for the detection of these hormones. The concentration of serotonin in the blood was determined by the fluorometric method [55][56] whereas the concentration of cortisol in the blood serum was determined via the enzyme-linked immunoassay using the

Luminescent gold nanoclusters for bioimaging applications

• Nonappa

Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42

• ; biosensing; gold nanoclusters; immunoassay; luminescence; self-assembly; theranostics; Introduction Imaging methods play a central role in understanding the structural and functional biological processes of biomolecules, cells, tissues, organs, and even entire living organisms [1][2]. The importance of

• a fluorescent gold nanocluster immunoassay (AuNCIA) for early and sensitive detection of human immune deficiency virus (HIV) infection in vitro and HIV-infected patient samples [85]. For this study, glutathione-capped AuNCs were coupled with streptavidin (Au-SA) using EDS/NHS coupling. The strong

• noncovalent interaction between streptavidin and biotin was exploited. To achieve the immunoassay, an antibody–antigen–antibody sandwich approach was utilized (Figure 3). The substrates were first coated with capture antibodies that will interact strongly with HIV-1 p24 antigen, a target viral protein

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

• Gennady L. Burygin,
• Polina I. Abronina,
• Nikita M. Podvalnyy,
• Sergey A. Staroverov,
• Leonid O. Kononov and
• Lev A. Dykman

Beilstein J. Nanotechnol. 2020, 11, 480–493, doi:10.3762/bjnano.11.39

• immunoassay (titrated twice in the microtiter plate) as follows. An aliquot (1 μL) of solution of each antigen (1 or 2) in the double dilutions (initial concentration, 1 mg·mL−1) was spotted onto a Westran S polyvinylidene fluoride (PVDF) membrane (Whatman), and the membrane was incubated in a dry-air

Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

• Camila A. Proença,
• Tayane A. Freitas,
• Thaísa A. Baldo,
• Elsa M. Materón,
• Flávio M. Shimizu,
• Gabriella R. Ferreira,
• Frederico L. F. Soares,
• Ronaldo C. Faria and
• Osvaldo N. Oliveira Jr.

Beilstein J. Nanotechnol. 2019, 10, 2171–2181, doi:10.3762/bjnano.10.210

• ]. PSA and interleukin 6 (IL-6) were measured with a microfluidic electrochemical immunoassay system, in which commercial magnetic particles were conjugated with secondary antibodies and horseradish peroxidase (HRP) [26]. These immunomagnetic nanoparticle-based microfluidic sensors with screen-printed

• washed with 400 μL of 0.5% BSA and 20 mol·L−1 of PBS buffer pH 7.4. It was then resuspended to 125 μL. In the fourth and last step, the bioconjugate complex was used to form the sandwich-type immunosensor, with 125 μL of the final solution used in the immunoassay to fill a 100 μL sample loop. This was

Nanoantenna-assisted plasmonic enhancement of IR absorption of vibrational modes of organic molecules

• Alexander G. Milekhin,
• Olga Cherkasova,
• Sergei A. Kuznetsov,
• Ilya A. Milekhin,
• Ekatherina E. Rodyakina,
• Alexander V. Latyshev,
• Sreetama Banerjee,
• Georgeta Salvan and
• Dietrich R. T. Zahn

Beilstein J. Nanotechnol. 2017, 8, 975–981, doi:10.3762/bjnano.8.99

• and in several tissues and regulates blood glucose levels. Cortisol production disorders lead to the development of diabetes, Cushing's syndrome and other pathologies [36]. Therefore, the determination of the cortisol concentration in blood and tissue is important. The immunoassay methods are

Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA)

• Paula Ciaurriz,
• Fátima Fernández,
• Edurne Tellechea,
• Jose F. Moran and
• Aaron C. Asensio

Beilstein J. Nanotechnol. 2017, 8, 244–253, doi:10.3762/bjnano.8.27

• single recognition reaction [6][8]. Luo and co-workers showed better sensitivities and shortened times for the detection of C-reactive proteins by using a quantum-dot-labeled immunoassay [13]. Accordingly, an improvement in sensitivity of 5,000 times for the detection of the ataxia telangiectasia mutated

Formation of substrate-based gold nanocage chains through dealloying with nitric acid

• Ziren Yan,
• Ying Wu and
• Junwei Di

Beilstein J. Nanotechnol. 2015, 6, 1362–1368, doi:10.3762/bjnano.6.140

• to their solid counterparts because of the high surface area, low density, and near-infrared localized surface plasmon resonance (LSPR). All these make Au NC an attractive material for various applications in optical [4][5] and electrochemical sensing [6], immunoassay [7], drug release [8], surface