Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension

• Khosro Adibkia,
• Ali Ehsani,
• Asma Jodaei,
• Ezzatollah Fathi,
• Raheleh Farahzadi and
• Mohammad Barzegar-Jalali

Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62

• -well plates were cultured in the cardiomyogenic differentiation medium for 14 days. The medium was refreshed twice weekly. Cardiomyogenic differentiation was confirmed by ICC. Immunofluorescence staining BM-MSCs were seeded in 8-well slide containing cardiomyogenic differentiation medium for 14 days

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

• Barbora Svitkova,
• Vlasta Zavisova,
• Veronika Nemethova,
• Martina Koneracka,
• Miroslava Kretova,
• Filip Razga,
• Monika Ursinyova and
• Alena Gabelova

Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22

• ). Single-cell clones were selected and amplified by dilution cloning in 6-well plates. Immunofluorescence staining Cells were grown on glass coverslips in 48-well plates overnight before incubation with Alexa Fluor 594–Transferrin conjugate (25 µg/mL) or cholera Toxin B subunit–FITC conjugate (5 mg/mL) at

Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior

• Yan Liu,
• Li Li,
• Xing Chen,
• Ying Wang,
• Meng-Nan Liu,
• Jin Yan,
• Liang Cao,
• Lu Wang and
• Zuo-Bin Wang

Beilstein J. Nanotechnol. 2019, 10, 2329–2337, doi:10.3762/bjnano.10.223

• are revealed by AFAM. Our results show that AFAM is capable of imaging surface elastic deformations. By immunofluorescence experiments, we find that the L929 cells significantly elongate on the patterned stiffness substrate, whereas the elasticity of the pattern has only little effect on the spreading

• measured by AFAM at room temperature. Immunofluorescence and image analysis After 48 h, the L929 cells were fixed with 4% (w/v) paraformaldehyde solution for 15 min. Subsequently, the cells were washed three times with PBS. The cytoskeletons of the L929 cells were stained green by phalloidin (Alexa Fluor

Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

• Aniela Bittner,
• Angélique D. Ducray,
• Hans Rudolf Widmer,
• Michael H. Stoffel and
• Meike Mevissen

Beilstein J. Nanotechnol. 2019, 10, 941–954, doi:10.3762/bjnano.10.95

• Figure 4B. Neither Si- nor Au-NPs led to differences in activation or expression of NF-κB. Both forms could be detected for this protein (Figure 4C). Expression of tight-junction proteins in rBCEC4 cells Immunofluorescence staining and TEM were used to demonstrate the expression of important BBB

• possible effect of NP exposure on TJ formation and established TJs was investigated using immunofluorescence staining for ZO-1 (Figure 5D–G). rBCEC4 cells were exposed to PLLA-NPs at a concentration of [24.9 µg/mL] at various time points during and after monolayer and barrier formation. No differences in

• signal intensity or continuity of ZO-1 between control cells and any of the conditions of PLLA exposure were detected. PCL-NP exposure did not elicit changes in immunofluorescence staining of ZO-1 either (data not shown). No variations in protein levels of ZO-1 and TJ protein claudin 3 were observed

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

• Reika Makita,
• Tsukasa Akasaka,
• Seiichi Tamagawa,
• Yasuhiro Yoshida,
• Saori Miyata,
• Hirofumi Miyaji and
• Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

• with a width or diameters of 500 nm. Immunofluorescence staining after 1 day of culturing Immunofluorescence images of Saos-2 cells on the gelatin patterns are shown in Figure 6. Vinculin (green), F-actin (red), and nuclei (blue) of the cells were stained after 1 day of culturing. The cells were

• . Immunofluorescence staining after 7 days of culturing Immunofluorescence images of Saos-2 cells on the gelatin patterns after 7 days of culturing are shown in Figure 9. The images of vinculin (green), F-actin (red), and nuclei (blue) were quite similar to the images obtained after 1 day of culturing (Figure 6

• attached to the upper surface surrounding the hole were also observed with immunofluorescence microscopy (Figure 9d and Figure 9e). It therefore appears that Saos-2 cells prefer the upper surface of gelatin holes rather than the interior of the hole. The shape of the cell body and the number of filopodia

Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

• Jennifer E. Francis,
• David Mason and
• Raphaël Lévy

Beilstein J. Nanotechnol. 2017, 8, 1238–1249, doi:10.3762/bjnano.8.125

• targets in the cytosol and could not reach the nucleus, even after extensive cell permeabilisation. Keywords: imaging; immunofluorescence; microscopy; nanoparticles; quantum dots; Introduction Quantum dots (Qdots) are nanometre-sized semiconductor nanocrystals that typically consist of a metallic core

• ligands (such as poly(ethylene glycol) (PEG) [3][4]) or they are encapsulated in amphiphilic polymers [5]. Antibodies that recognise specific biological targets can then be conjugated to these Qdots for use in immunofluorescence. Conventional immunocytochemistry (ICC) protocols involve the chemical

• of some of the few Qdot conjugates commercially available for immunofluorescence (Table S1 in Supporting Information File 1). While all tested antigens could be labelled using antibodies conjugated to fluorescent dyes (Dye-Abs) (e.g. Alexa Fluor 488), only tubulin and extracellular fibronectin could

Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• , arrays were washed with PBS containing 10−4 DHT to maintain the AR stability. Binding of ARbiot was detected via indirect immunofluorescence. First samples were blocked with fetal calf serum (FCS) for 1 h at room temperature (RT), then washed with PBS containing 10−4 DHT and loaded for 1 h with anti-AR

• immunofluorescence (FITC channel). Binding of STV-Cy3 served as an optical reference to easily localize the lipid arrays on the sample. Fluorescence signal after antibody binding on ARbiot coated arrays is homogenous, indicating an even distribution of the receptor bound to STV-biotin arrays embedded in the HEMA

• against washing steps, while still being accessible for protein and antibody binding. Even complex receptor protein structures of ARbiot remain intact upon binding to the lipid arrays and are still accessible for the indirect immunofluorescence detection. The described model system and its demonstrated

Low temperature co-fired ceramic packaging of CMOS capacitive sensor chip towards cell viability monitoring

• Niina Halonen,
• Joni Kilpijärvi,
• Maciej Sobocinski,
• Timir Datta-Chaudhuri,
• Antti Hassinen,
• Someshekar B. Prakash,
• Peter Möller,
• Pamela Abshire,
• Sakari Kellokumpu and
• Anita Lloyd Spetz

Beilstein J. Nanotechnol. 2016, 7, 1871–1877, doi:10.3762/bjnano.7.179

• cells grow on top of the chip. (d–f) The cells grow on top of LTCC. In (a) and (d), the blue color indicates the cell nuclei stained with a DNA binding dye, Hoechst 33342. In (b) and (e), immunofluorescence staining was performed with anti-α-tubulin antibody and Alexa 488 secondary antibody. The green

Pulmonary surfactant augments cytotoxicity of silica nanoparticles: Studies on an in vitro air–blood barrier model

• Jennifer Y. Kasper,
• Lisa Feiden,
• Maria I. Hermanns,
• Christoph Bantz,
• Michael Maskos,
• Ronald E. Unger and
• C. James Kirkpatrick

Beilstein J. Nanotechnol. 2015, 6, 517–528, doi:10.3762/bjnano.6.54

• spectrometer (Ex/Em wavelength 494/518 nm). Inflammatory responses: As described in our previous studies [9][10][11] the supernatants were taken to determine IL-8 release via ELISA (DuoSet R&D, DY208) following the manufacturer’s recommendations. Immunofluorescence (IF): Cells were fixed with paraformaldehyde

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

• Anja Ostrowski,
• Daniel Nordmeyer,
• Alexander Boreham,
• Cornelia Holzhausen,
• Lars Mundhenk,
• Christina Graf,
• Martina C. Meinke,
• Annika Vogt,
• Sabrina Hadam,
• Jürgen Lademann,
• Eckart Rühl,
• Ulrike Alexiev and
• Achim D. Gruber

Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25

• ]. As a consequence, NP can only be detected when they are closely clustered in aggregates or densely packed, for example, in phagocytic vacuoles. Pathologists routinely employ methods of fluorescent labeling for immunofluorescence, i.e., antibody-based identification of specific cell types, cell

The distribution and degradation of radiolabeled superparamagnetic iron oxide nanoparticles and quantum dots in mice

• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Markus Heine,
• Christian Waurisch,
• Gordon M. Stachowski,
• Stephen G. Hickey,
• Alexander Eychmüller,
• Jörg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 111–123, doi:10.3762/bjnano.6.11

• function by expressing several types of scavenger receptors and that Kupffer cells (KCs) belong to the family of macrophages and form part of the reticuloendothelial system. Thus, the sections were analyzed by immunofluorescence and stained for hepatic endothelial cells and Kupffer cells, which are known

• or the cells were allowed to rest for another 24 h before fixing for immunofluorescence studies. Fluorescent immunostaining For immunostaining, the cryosection slides were blocked with 1% BSA in PGS (PBS, 0.5 mg/mL saponin, 5 mg/mL glycine) for 1 h at room temperature. After blocking, the slides were

• incubated with the respective primary antibodies at 4 °C overnight. Afterwards, the slides were washed three times with PGS followed by incubation with secondary antibodies for 2 h at room temperature. For in vitro immunofluorescence studies, cells were likewise blocked with 1% BSA in PGS for 1 h at room

Localized surface plasmon resonances in nanostructures to enhance nonlinear vibrational spectroscopies: towards an astonishing molecular sensitivity

• Dan Lis and
• Francesca Cecchet

Beilstein J. Nanotechnol. 2014, 5, 2275–2292, doi:10.3762/bjnano.5.237

• , which often reduces image contrast in common immunofluorescence images. These properties promoted SE-CARS and CARS microscopy as a new tool for biomedical analysis. Still in 2011, Namboodiri et al. investigated the SERS and SE-CARS contribution from a mixture of pyridine molecules and silver colloids

Carbon-based smart nanomaterials in biomedicine and neuroengineering

• Antonina M. Monaco and
• Michele Giugliano

Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196

• . The presence of these intimate connections was then proved, by means of transmission electron microscopy (TEM), high resolution scanning electron microscopy (HRSEM) and immunofluorescence confocal laser scanning (CLS) microscopy, also conducted by Sorkin et al. [120], who suggested a correlation

The cell-type specific uptake of polymer-coated or micelle-embedded QDs and SPIOs does not provoke an acute pro-inflammatory response in the liver

• Markus Heine,
• Alexander Bartelt,
• Oliver T. Bruns,
• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Ludger Scheja,
• Christian Waurisch,
• Alexander Eychmüller,
• Rudolph Reimer,
• Horst Weller,
• Peter Nielsen and
• Joerg Heeren

Beilstein J. Nanotechnol. 2014, 5, 1432–1440, doi:10.3762/bjnano.5.155

• amphiphilic polymer poly(maleic anhydride-alt-1-octadecene) (PMAOD). The goal of the current study is to investigate the uptake processes as well as pro-inflammatory responses in the liver after the injection of these encapsulated nanocrystals. By immunofluorescence and electron microscopy studies using wild

The softening of human bladder cancer cells happens at an early stage of the malignancy process

• Jorge R. Ramos,
• Joanna Pabijan,
• Ricardo Garcia and
• Malgorzata Lekka

Beilstein J. Nanotechnol. 2014, 5, 447–457, doi:10.3762/bjnano.5.52

• microtubules (MT) in AFM measurements remains open. Pelling et al. performed immunofluorescence and AFM studies in order to determine the influence of the MT on the cell membrane in response to serum conditions and nocodazole [17]. Their results show that the stiffness depends on the interplay between

Atomic force microscopy recognition of protein A on Staphylococcus aureus cell surfaces by labelling with IgG–Au conjugates

• Elena B. Tatlybaeva,
• Hike N. Nikiyan,
• Alexey S. Vasilchenko and
• Dmitri G. Deryabin

Beilstein J. Nanotechnol. 2013, 4, 743–749, doi:10.3762/bjnano.4.84

• protein A (SpA) to bind in the Fc region of IgG. At the same time, the observed result was comparable to the immunolabelling methodology based on the affinity of SpA for IgG, which is applicable to either immunofluorescence observation using light microscopy or immunogold detection with electron