Hierarchically patterned polyurethane microgrooves featuring nanopillars or nanoholes for neurite elongation and alignment

• Lester Uy Vinzons,
• Guo-Chung Dong and
• Shu-Ping Lin

Beilstein J. Nanotechnol. 2023, 14, 1157–1168, doi:10.3762/bjnano.14.96

• had a rounded bottom. The space between the pillars and holes were around 860 nm and 330 nm, respectively. The wettability of a surface is a good predictor of protein adsorption and bioactivity [20]. For the extracellular matrix protein laminin, good adsorption and cell growth have been found on

• increase in CA on the nanopillar and nanohole substrates may be due to either a Wenzel- or a Cassie-type of wetting [22]. To improve wetting on the substrates, we treated our samples with mild O2 plasma before laminin incubation. After plasma treatment, all samples became hydrophilic (CA < 80°), with the

• nanopillar substrate having the smallest CAs (CA ≈ 30°). Based on confocal fluorescence microscopy of immunostained samples (Figure 1J–L), laminin successfully adsorbed onto the O2 plasma-treated PU samples. There was good laminin coverage on all of the samples, even on the nanostructures, as indicated by

Bioselectivity of silk protein-based materials and their bio-inspired applications

• Hendrik Bargel,
• Vanessa T. Trossmann,
• Christoph Sommer and
• Thomas Scheibel

Beilstein J. Nanotechnol. 2022, 13, 902–921, doi:10.3762/bjnano.13.81

• bioadhesiveness of a material is the tripeptide RGD, naturally occurring in various ECM proteins including fibronectin, vitronectin, laminin, and collagen [34][35][37][38][39][40][41]. This frequently used peptide has been shown to increase the bioadhesiveness of biomaterials by interacting with cellular integrin

• peptides to promote cellular interactions with biomaterials are the pentapeptides IKVAV (Ile–Lys–Val–Ala–Val) and YIGSR (Tyr–Ile–Gly–Ser–Arg), which are naturally present in laminin and are used for increasing bioadhesiveness and cellular interactions with biomaterials [23][33][34][35][41][46][47][48

• fact that the proteins in the ECM (such as fibronectin, vitronectin, collagen, and laminin) are adsorbed in a denatured state, and their orientation becomes unsuitable for cell binding [66]. Cells (e.g., osteoblasts) are more likely to attach to more hydrophilic surfaces and exhibit the strongest

Engineered titania nanomaterials in advanced clinical applications

• Padmavati Sahare,
• Paulina Govea Alvarez,
• Juan Manual Sanchez Yanez,
• Gabriel Luna-Bárcenas,
• Samik Chakraborty,
• Sujay Paul and
• Miriam Estevez

Beilstein J. Nanotechnol. 2022, 13, 201–218, doi:10.3762/bjnano.13.15

• affinities for the nanoparticle surface or the corona. Recently, Zhongru Gou et al. investigated the amount and type of cell adhesion-related proteins (such as fibronectin, vitronectin, and laminin) from serum adsorbed on titanium nanotube arrays. Their findings suggest that all the abovementioned proteins

Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

• Varsha Sharma and
• Anandhakumar Sundaramurthy

Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41

• in multilayer films such as PGA/PLL, PAH/PAAm and hyaluronic acid (HA)/chitosan when one of the polymeric pair is grafted with an arginine–glycine–aspartic acid (RGD) peptide sequence [104][105][106]. Similarly, the PLL/PGA film showed better cell attachment when plain PGA was replaced with a laminin

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

• Alexandra M. Greiner,
• Adria Sales,
• Hao Chen,
• Sarah A. Biela,
• Dieter Kaufmann and
• Ralf Kemkemer

Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155

• as fibronectin, laminin, collagen and vitronectin [151][152][153][154]. One has to keep in mind, that in most in vitro and particularly in in vivo environments plenty of different proteins are getting in contact with the surface and alter the initial coating, thus making it difficult to maintain

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

• Igor M. Pongrac,
• Marina Dobrivojević,
• Lada Brkić Ahmed,
• Michal Babič,
• Miroslav Šlouf,
• Daniel Horák and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84

• maintain their neural progenitor phenotype as well as their neural differentiation potential. In order to confirm the progenitor identity of NSCs labeled with PLL-γ-Fe2O3 or nanomag®-D-spio nanoparticles, NSCs were grown on PDL/laminin-coated glass slides for 24 h, labeled with nanoparticles for 48 h and

• left to proliferate for additional 48 h in fresh media with FGF and EGF. In order to address their differentiation potential, NSCs were grown on PDL/laminin-coated glass slides for 24 h, labeled for 48 h with PLL-γ-Fe2O3 or nanomag®D-spio nanoparticles and allowed to differentiate in fresh media

Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces

• Miao Yu,
• Nico Strohmeyer,
• Jinghe Wang,
• Daniel J. Müller and
• Jonne Helenius

Beilstein J. Nanotechnol. 2015, 6, 157–166, doi:10.3762/bjnano.6.15

• matrix proteins, fibronectin, collagen I and laminin 332, was examined. The adhesion of each cell line to different matrix proteins was found to be distinct; no two cell lines adhered equally to each of the proteins. The PDMS masks improved the throughput limitation of single-cell force spectroscopy and

• allowed for experiments that previously were not feasible. Since the masks are economical and versatile, they can aid in the improvement of various assays. Keywords: atomic force microscopy; cell adhesion; collagen I; fibroblasts; fibronectin; HeLa; laminin; MDCK; PC3; single cell assay; single cell

• efficiency. We implemented the masks to characterize the adhesion of different cell lines to collagen I, fibronectin and laminin 332 and found that the cell lines had unique adhesion profiles, which likely reflect differences in the CAMs they expressed. Results PDMS masks for single-cell force spectroscopy

Carbon-based smart nanomaterials in biomedicine and neuroengineering

• Antonina M. Monaco and
• Michele Giugliano

Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196

• al. [125] were among the first to report on the suitability of ND as a substrate material. By micro-contact printing, they deposited laminin on NDs to form patterned microstructures, on which they then observed the ordered growth of primary cortical neurons. Neuronal adhesion and cell excitability

• been studied by Park et al. [144]; the results showed not only an improved differentiation but also an enhanced cell adhesion and neurites formation compared to control conditions. Moreover, the expression of laminin-related receptors and of genes involved in the calcium signalling pathway was up

Growth and structural discrimination of cortical neurons on randomly oriented and vertically aligned dense carbon nanotube networks

• Christoph Nick,
• Sandeep Yadav,
• Ravi Joshi,
• Christiane Thielemann and
• Jörg J. Schneider

Beilstein J. Nanotechnol. 2014, 5, 1575–1579, doi:10.3762/bjnano.5.169

• 500,000 cells/mm2 onto each substrate and incubated in a humidified atmosphere for 4 h. Finally, petri dishes were filled with approximately 3 mL culture medium. Half the medium was replaced twice a week and completely replaced every second week. In the cell culture protocol no coating (e.g., laminin or

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• , for instance fibronectin, vitronectin, fibrinogen or laminin, contain the tripeptide sequence arginine-glycine-aspartic acid (RGD), which is specifically recognised by most integrins [3][7][8]. The affinity as well as the specificity of integrins to bind to their ligands in the ECM can also be

Fabrication of multi-parametric platforms based on nanocone arrays for determination of cellular response

• Lindarti Purwaningsih,
• Tobias Schoen,
• Tobias Wolfram,
• Claudia Pacholski and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2011, 2, 545–551, doi:10.3762/bjnano.2.58

• on large areas. Gold nanoparticles on top of the nanostructures allow for spatially resolved functionalization with a variety of biomolecules through simple thiol chemistry. A 3,3'-dithiobis(sulfosuccinimidylpropionate) (DTSSP) linker molecule was used to immobilize laminin, an extracellular matrix

• , multidomain, trimeric glycoprotein, on the differently structured substrates. Laminin is known to support neural cell adhesion, proliferation, and differentiation. The cell adhesion activity of human neuroblastoma cells (SHSY-5Y) was investigated on these substrates and correlated with topographical features

• three different diblock copolymer chain lengths, before (first row) and after surface functionalization with DTSSP and laminin (second row). In general, the contact angles and therefore the hydrophobicities of the three fabricated nanostructures are quite similar before functionalization (~60